• Journal of Animal Science and Technology
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• 제66권1호
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• pp.156-166
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• 2024

Pigs are genetically, anatomically, and physiologically similar to humans. Recently, pigs are in the spotlight as a suitable source animal for xenotransplantation. However, to use pigs as source animals, pigs should be raised in designated pathogen-free facilities. There is abundant data from embryo transfer (ET) experiments using farm pigs as surrogates, but data on ET experiments using minipigs are scarce. Eighty minipigs were used for ET experiments and after transplantation, the implantation and delivery rates were investigated. It was also confirmed whether the pregnancy rate could be increased by changing the condition or surgical method of the surrogate. In the case of minipigs that gave birth, the size of the fetal sac on the 28th day of ET was also measured. The factors that can affect the pregnancy rate such as estrus synchronization program, ovulation status at the time of ET, the number of repeated ET surgeries, and the ET sites, were changed, and the differences on the pregnancy rate were observed. However there were no significant differences in pregnancy rate in minipigs. The diameter of the implanted fetal sac on the 28th day after ET in the minipigs whose delivery was confirmed was calculated to be 4.7 ± 0.5 cm. In conclusion, there were no significant differences in pregnancy rate of minipigs in the comparative experiment on various factors affecting the pregnancy rate. However, additional experiments and analyses are needed due to the large individual differences of the minipigs.

• 한국수정란이식학회지
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• 제23권3호
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• pp.167-175
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• 2008

This study was performed to investigate the result that in-vivo or in-vitro embryos of Hanwoo cows were transferred to Holstein cows. Seventeen Hanwoo cows were used as donors for production of in-vivo embryos and fresh hanwoo in-vivo embryos were transferred to 1,150 Holsteins. And 2 embryos were transferred to 188 Holstein recipients to produce twin calves. Diagnosis on pregnancy was performed by rectal palpation at $60\sim90$ days after transfer. The pregnancy rate of Holstein recipients was 55.8% after transfer with Hanwoo in-vivo embryos and 38.2% after transfer with Hanwoo in-vitro embryos. The delivery rate of pregnant Holstein recipients was 88.4% after transfer with Hanwoo in-vivo embryos and 75.6% after transfer with Hanwoo in-vitro embryos. The rate of delivery of Holstein recipients transferred with two Hanwoo embryos was 36.2% and the rate of twin production was 25.9%. The rate of twin production by embryo transfer with in-vivo embryos was 30.4%, whereas the fate with in-vitro embryos was 15.6%. The pregnancy rate according to the grade of corpus luteum of Holstein recipients transferred with Hanwoo in-vitro embryos was 41.5 and 36.0% for A and B grade, respectively. The pregnancy rate according to the transfer in site in the uterine lumen of recipients was 40.9 and 32.7% for anterior and middle site, respectively. The pregnancy rate according to day of embryo transfer after estrus of recipients was 45.5, 38.8 and 39.7% for day 6, day 7 and day 8, respectively. There was difference of pregnancy rate according embryo transfer technician ($30.5\sim45.8%$) individual dairy farm ($21.1\sim51.0%$). These results are supposed to indicate that the rate of pregnancy after transfer with Hanwoo embryos to Holstein recipients was similar to that within the same breed, and consequently that this method would be beneficial to enhance the productivity in Hanwoo reproduction.

• 한국임상수의학회지
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• 제26권4호
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• pp.376-378
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• 2009

2007년 8월 26일 생인 7개월된 숫송아지에게 음경전위술을 실시했다. 음경의 방향을 바꿔 정상적인 교미를 불가능하게 함으로써 경산우와 처녀우군에 도입이 되면 후각을 이용하여 발정을 탐지하게 되고 수의사에게 인공수정 혹은 수정란이식의 최적시간을 제공하게 될 것이다. 본 보고는 어린 숫송아지에게 실시되는 음경전위술법을 설명한 것이다.

• Journal of Dairy Science and Biotechnology
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• 제39권1호
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• pp.1-8
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• 2021

The surroundings of livestock farms, including dairy farms, are known to be a major source of development and transmission of antibiotic-resistant bacteria. To control antibioticresistant bacteria in the livestock breeding environment, farms have installed livestock wastewater treatment facilities to treat wastewater before discharging the final effluent in nearby rivers or streams. These facilities have been known to serve as hotspots for inter-bacterial antibiotic-resistance gene transfer and extensively antibiotic-resistant bacteria, owing to the accumulation of various antibiotic-resistant bacteria from the livestock breeding environment. This review discusses antibiotic usage in livestock farming, including dairy farms, livestock wastewater treatment plants as hotspots for antibiotic resistant bacteria, and nonenteric gram-negative bacteria from wastewater treatment plants, and previous findings in literature.

• 대한임베디드공학회논문지
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• 제18권1호
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• pp.19-24
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• 2023

In traditional fish farming way, the workers have to observe all of the pools every time and every day to feed at the right timing. This method causes tremendous stress on workers and wastes time. To solve this problem, we implemented an automatic detection system for feeding time using deep neural network. The detection system consists of two steps: classification of the presence or absence of feed and checking DO (Dissolved Oxygen) of the pool. For the classification, the pretrained ResNet18 model and transfer learning with custom dataset are used. DO is obtained from the DO sensor in the pool through HTTP in real time. For better accuracy, the next step, checking DO proceeds when the result of the classification is absence of feed several times in a row. DO is checked if it is higher than a DO reference value that is set by the workers. These actions are performed automatically in the UI programs developed with LabVIEW.

• 한국전자통신학회논문지
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• 제17권5호
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• pp.1003-1012
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• 2022

풍력발전시스템의 터빈과 하부구조에 전달되는 수평방향의 에너지 전달은 시스템의 안전성 유지 측면에서 매우 중요한 요소이지만 지진, 태풍과 같은 대규모 연안재해에 취약할 수 밖에 없다. 연안 또는 먼 해상에 구축되는 풍력발전시스템은 연안재해에 취약한 지역에 설치 시 보다 견고한 설계가 요구되기 때문에 초기 투자비용의 증가로 사업의 경제성 측면에서 매우 불리하다. 본 연구에서는 연안재해의 리스크를 저감한다는 관점에서 풍력발전단지의 최적 부지를 선정하기 위해 GIS 기법을 사용하였다. 우리나라 서해와 남해의 지진 현황, 서해와 남해에 영향을 미치거나 통과하는 태풍의 이동경로와 강도도 복합적으로 분석하였다. 이에 연안재해 위험이 가장 낮은 최적의 해상풍력단지 부지를 선정했고, 향후 해당지역 해상풍력 프로젝트의 기초연구자료로 활용하고자 한다.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.128-128
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• 2003

The aims of this study are 1) to test oocytes and embryos collected from in-vivo and in-vitro to achieving the valuable protocol by culturing, vitrifying and thawing of oocytes/embryos, and 2) to transfer them to recipient, and finally have resulted in pregnancies from recipient females after surgical or nonsurgical transfer. In vitro maturation and fertilization were performed according to the procedures of Funahashi et al. Fertilized oocytes were cultured in glucose-free NCSU 23 supplemented with 5 mM sodium pyruvate, 0.5 mM sodium lactate and 4 mg/ml bovine serum albumin for 2 days at 39$^{\circ}C$, and 10% fetal bovine serum was added to the culture medium thereafter. Embryos were treated with 7.5$\square$g/ml cytochalasin-B for 30 min, centrifuged at 13,000 ${\times}$ g for 13 min and then exposed sequentially to an ethylene glycol (EG) vitrification solution, aspirated into OPSs, and plunged/thawed into/from liquid nitrogen. In vivo embryos were surgically collected from three donors after Al. Forty-six embryos (18, 9 and 19 embryos, respectively) were washed 3 times in mPBS+10%FBS, followed treatments : cultured, centrifuged, vitrified, recovered and transferred to recipients as in vitro prepared embryos. Three recipients received surgically 34(control), 188 and 184 embryos (derived from abattoir), respectively. Another three recipients were received nonsurgically 150, 100 and 150 embryos, respectively. All recipient sows exhibited delayed returns to estrus. To our knowledge, these results suggest that required an improved techniques, more vigorous embryos preparation and cleaner uterous condition(use gilt).

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1210-1210
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• 2001

On farm analysis of protein, moisture and oil in cereals and oil seeds is quickly being adopted by Australian farmers. The benefits of being able to measure protein and oil in grains and oil seeds are several : $\square$ Optimize crop payments $\square$ Monitor effects of fertilization $\square$ Blend on farm to meet market requirements $\square$ Off farm marketing - sell crop with load by load analysis However farmers are not NIR spectroscopists and the process of calibrating instruments has to the duty of the supplier. With the potential number of On Farm analyser being in the thousands, then the task of calibrating each instrument would be impossible, let alone the problems encountered with updating calibrations from season to season. As such, NIR technology Australia has developed a mechanism for \ulcorner\ulcorner\ulcorner their range of Cropscan 2000G NIR analysers so that a single calibration can be transferred from the master instrument to every slave instrument. Whole grain analysis has been developed over the last 10 years using Near Infrared Transmission through a sample of grain with a pathlength varying from 5-30mm. A continuous spectrum from 800-1100nm is the optimal wavelength coverage fro these applications and a grating based spectrophotometer has proven to provide the best means of producing this spectrum. The most important aspect of standardizing NIB instruments is to duplicate the spectral information. The task is to align spectrum from the slave instruments to the master instrument in terms of wavelength positioning and then to adjust the spectral response at each wavelength in order that the slave instruments mimic the master instrument. The Cropscan 2000G and 2000B Whole Grain Analyser use flat field spectrographs to produce a spectrum from 720-1100nm and a silicon photodiode array detector to collect the spectrum at approximately 10nm intervals. The concave holographic gratings used in the flat field spectrographs are produced by a process of photo lithography. As such each grating is an exact replica of the original. To align wavelengths in these instruments, NIR wheat sample scanned on the master and the slave instruments provides three check points in the spectrum to make a more exact alignment. Once the wavelengths are matched then many samples of wheat, approximately 10, exhibiting absorbances from 2 to 4.5 Abu, are scanned on the master and then on each slave. Using a simple linear regression technique, a slope and bias adjustment is made for each pixel of the detector. This process corrects the spectral response at each wavelength so that the slave instruments produce the same spectra as the master instrument. It is important to use as broad a range of absorbances in the samples so that a good slope and bias estimate can be calculated. These Slope and Bias (S'||'&'||'B) factors are then downloaded into the slave instruments. Calibrations developed on the master instrument can then be downloaded onto the slave instruments and perform similarly to the master instrument. The data shown in this paper illustrates the process of calculating these S'||'&'||'B factors and the transfer of calibrations for wheat, barley and sorghum between several instruments.

• 한국수정란이식학회지
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• 제29권1호
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• pp.29-33
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• 2014

재래가축의 유전자원(FAnGH)의 유전학적 및 표현형적인 특성과 다양성에 대한 정확한 정보는 가축의 현재와 미래의 지속 가능한 이용 및 보존에 중요한 역할을 한다. 본 논문은 과제에 참가한 각 나라별로 목적, 구조, 기능, 내용, 이용성 그리고 미래의 전망 등을 국제축산연구소의 각국별 재래가축 유전자원 정보시스템(DAGRIS)이라는 데이터베이스에 기술하였다. 이 DAGRIS는 전자 데이터베이스로 개발도상국의 연구자, 정책 담당자, 개발 실행자, 교육자, 학생 그리고 농업인들의 요구에 부합되게 설계되었다. 또한 각국의 선발된 가축유전자원(FAnGR)의 기원, 분포, 다양성, 현재 사용도 및 상태 등에 관한 과거와 현재의 연구 결과로부터 논문으로 게재되거나, 문헌 등을 쉽게 접근하기 좋게 개발되었으며, 영어와 불어 사용자를 위하여 영어와 불어로 c-DAGRIS의 국가별 모듈을 개발하고 사용될 수 있게 만들었다.

• Asian-Australasian Journal of Animal Sciences
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• 제17권2호
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• pp.168-173
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• 2004

The ability of frozen-thawed fetal skin was examined to generate viable cell lines for nuclear transfer. Fetal skin frozen at -20$-20^{\circ}C$, $-30^{\circ}C$ or $-80^{\circ}C$ in the presence of 5% DMSO used as tissue explants to generate somatic cells. The resultant confluent cells were then used as donors for nuclear transfer (NT). Of the bovine NT embryos reconstracted from the somatic cells, 62.3%, 76.6% to 65% showed cleavage 70.5%, 81.9% to 78.5% reached the stage of morula formation and 39.7%, 43.2% or 47.6% reached the blastocyst stage. There was no significant difference in development when the NT embryos were compared with those reconstracted from fresh somatic cell derieved skin tissues (72%, 75.3%, and 45.2%, for cleavage, and development to morula and blastocyst stage, respectively). NT embryos were then placed in a portable $CO_2$ incubator and carried to China from Japan by air. After reaching to farm, two NT embryos were transferred to each of 5 recipients. We obtained 2 NT calves which birth weights is 30kg and 36kg female, and gestation periods is 281 and 284 days, respectively. There were no observation any abnormality from those calves. The results indicated that cell lines derieved from bovine fetal skin cryopreserved by a simple method could be used as donors in nuclear transfer using the portable $CO_2$ incubator.