• 대한간호학회지
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• 제4권1호
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• pp.22-37
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• 1974

Through the examination of hospitalized patient's records, this study purports to determine. the extent to which nursing record behaviors meet general expectations held for nursing records and the differences in nursing record behavior in relation to different nursing categories, period of recording and hospitals with different management patterns. Nursing record behaviors of 802 patient's records in four hospitals in Seoul were examined. by use of a check list, which was prepared by the author as an instrument for the study. Data obtained from this examination were processed into percentage values for percentage. test and chi-square test in order to determine their significance. Results are as follows; 1. Records pertaining to treatment ranked highest among all care categories in their extent of coverage, averaging 65.6 percent. 2. Of the treatment category records, records of medication led others at 94.3 percent. followed by records of test and collection of specimens at 59.9 percent. diet at 58.8 percent and treatment at 41.0 percent. 3. Records in the category of physical assessment and care averaged 44.1 percent, the second highest next to treatment category records, 4. Of the records in the category of physical assessment and care, records in vital signs. placed first at 98.9 percent, followed by sleep at 76.2 percent, body weight at 74.7 percent, symptoms and signs at 69.3 percent, rest at 44.5 percent, hygiene at 39.7 percent, activities and participation at 16.9 percent, positions at 10.3 percent, level of consciousness at 9.8 percent and physiological dysfunction at 1.1 percent in that order. 5. Records in the category of psychological assessment and care averaged 3.2 percent, the lowest of the -three major categories. 6. Of the records in the category of psychological assessment and care, records on emotional responses ranked top at 10.5 percent, followed by self-concern at 2.1 percent, adjustment at 2.0 percent, family, occupational and social relations at 0.7 percent and preferences. and interest at 0.5 percent in that order. 7. Records in relation to the category of specific conditions were found in 9.1 percent of the total records. 8. Of the records in the category of specific conditions, consultation and transfer records, stood first at 25.0 percent, followed by precautionary measurements at 1.4 percent and isolation at 0.9 percent 9. A great difference in nursing record behavior was observed between the first week of hospitalization and the last week, with the first week's recordings much higher than the last week in the categories of treatment and specific conditions (p<0.01). and of physical assessment and care (p <0.05). 10. A big difference was also observed among the hospitals (p<0.01). 11. A big difference was also observed between the government-run hospitals and the private hospitals in the categories of physical assessment and care and specific conditions in the first week of hospitalization (P<0.05l), and in the category of psychological assessment and care in the last week (P<0.05). 12. Between the hospitals established with foreign aid and the other hospitals, the difference in nursing record behavior was significant only in the category of physical assessment and care both in the first week and the last week (P<0.01). 13. The average nursing record behavior in all care categories stood at 45.1 percent in the extent of its coverage in relation to the general expectations.

• 한국언론정보학보
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• 제32권
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• pp.247-293
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• 2006

이 연구는 근대 의약품 광고에 드러난 신체 담론에 관한 연구이다. 최근 근대 형성기에 대한 미시적 접근은 일상사의 영역연구에서 두드러지는데, 일상적인 영역이야말로 사람들의 구체적인 경험이기 때문이다. 특히 근대 광고에는 당시 생활세계를 구성했던 대중문화, 소비문화, 성, 가족, 의 식 주, 질병 등의 구체적인 영역이 고스란히 담겨있다. 근대 이후 일상생활의 재조직화에서 신체는 이전까지 상속되고 신분적 질서에 의해 규정되었던 전근대적인 신체와 달리 어떤 규칙과 가치 속에서 자신의 신체를 자기 스스로 만들고 조립하여야 하는 근대적인 신체로 변화했다. 이 연구는 의약품 광고를 통해서 그 당시 사람들이 자신의 신체를 어떻게 인식하는지를 분석하고자 했고 분석방법으로 푸코의 담론형성이론을 활용하였다. 분석결과, 근대 초기의 의약품 광고 각각의 텍스트를 가로지르는 몇 가지 공통의 규칙, 즉 담론-장치를 찾을 수 있었고, 그 규칙성 속에서 의약품 광고가 근대적 아픔의 주체를 구성하는 과정을 살필 수 있었다. 근대 의약품 광고에 투영된 '임상의학적 시선의 내면화'와 '자기감시'라는 규율 권력적 요소는 '근대적 아픔의 주체' 형성에 기여했고, 사회 권력과 접합된 방식으로 표상되기도 했다.

• 대한한방내과학회지
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• 제37권1호
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• pp.65-76
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• 2016

Objectives: The purpose of this study was to identify the inhibitory effects of Hwangheuk-san (HHS), a Korean multi-herb formula comprising four medicinal herbs, on cell migration and invasion, two critical cellular processes that are often deregulated during metastasis, using the human bladder cancer 5637 cell line.Methods: Cell viability, motility, and invasion were assessed by 3-(4,5-dimethyl-2 thiazolyl)-2,5-diphnyl-2H-tetrazolium bromide (MTT), wound healing migration, and Transwell assays, respectively. Gene expression was detected by Western blot analysis. In addition, the activities of matrix metalloproteinases (MMPs) and the values for transepithelial electrical resistance (TER) were analyzed using a Gelatinase Activity Assay Kit and an Epithelial Tissue Voltohmmeter, respectively.Results: Our data indicated that within the concentration range that was not cytotoxic, HHS effectively inhibited the cell motility and invasiveness of 5637 cells. HHS markedly decreased the expression and activity of MMP-2 and MMP-9, which was associated with unregulation of tissue inhibitors of metalloproteinase (TIMP)-1 and TIMP-2. Further investigation revealed that phosphorylation of phosphatidylinositol 3-kinase (PI3K) and AKT was decreased in HHS-treated 5637 cells, and a PI3K/AKT inhibitor synergistically reduced the inhibition of migration and invasion and also inactivated MMP-2 and MMP-9. Moreover, HHS increased the tightening of tight junctions (TJs), which was demonstrated by an increase in the TER, and reduced the expression the levels of claudin family members (claudin-3 and -4), which are major components involved in the tightening of TJs.Conclusions: The present findings demonstrated that HHS attenuated the migration and invasion of bladder cancer 5637 cells by modulating the activity of the PI3K/Akt signaling pathway and also through TJ tightening.

• Biomolecules & Therapeutics
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• 제22권4호
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• pp.347-354
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• 2014

Larrea nitida is a plant that belongs to the Zygophyllaceae family and is widely used in South America to treat inflammatory diseases, tumors and menstrual pain. However, its pharmacological activity remains unclear. In this study we evaluated the property of selective estrogen receptor modulator (SERM) of Larrea nitida extracts (LNE) as a phytoestrogen that can mimic, modulate or disrupt the actions of endogenous estrogens, depending on the tissue and relative amount of other SERMs. To investigate the property of SERM of LNE, we performed MCF-7 cell proliferation assays, estrogen response element (ERE)-luciferase reporter gene assay, human estrogen receptor (hER) binding assays and in vivo uterotrophic assay. To gain insight into the active principles, we performed a bioassay-guided analysis of LNE employing solvents of various polarities and using classical column chromatography, which yielded 16 fractions (LNs). LNE showed high binding affinities for $hER{\alpha}$ and $hER{\beta}$ with $IC_{50}$ values of $1.20{\times}10^{-7}$ g/ml and $1.00{\times}10^{-7}$ g/ml, respectively. LNE induced $17{\beta}$-estradiol (E2)-induced MCF-7 cell proliferation, however, it reduced the proliferation in the presence of E2. Furthermore, LNE had an atrophic effect in the uterus of immature rats through reducing the expression level of progesterone receptor (PR) proteins. LN08 and LN10 had more potent affinities for binding on $hER{\alpha}$ and ${\beta}$ than other fractions. Our results indicate that LNE had higher binding affinities for $hER{\beta}$ than $hER{\alpha}$, and showed SERM properties in MCF-7 breast cancer cells and the rat uterus. LNE may be useful for the treatment of estrogen-related conditions, such as female cancers and menopause.

• 생태와환경
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• 제36권1호통권102호
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• pp.29-37
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• 2003

부산의 대표적인 자연형 계곡이 있는 범어사에서 도시하천인 온천천을 거쳐 수영강 합류부에 분포하는 저서성 대형무척추동물을 계절별로 조사하였다. 전 조사지점에서 층 4문 5강 10목 19과 23종이 조사되었다. 범어사에서는 하루살이류 (Ephemeroptera), 강도래류(Ple-coptera)등 다양한 분류군이 출현한 반면에 나머지 조사지에서 빈모류 (Oligochaeta)와 깔따구류 (Chirono-midae)가 우점하였다. 두 우점분류군의 출현시기는 7월12월과 3월은 빈모류군이 우점을 차지하였으나 9월은 깔따구류가 우점을 차지하여 차이를 보였다. 생물학적 지수인 TBI (Trent Biotic Index), BS (Biotic Score), BMWP (Biological Monitoring Working Party)를 조사하였는데 전체적으로 범어사 지점에서 하류로 가면서 각 지수들이 낮아지는 경향이 나타났다. 전체적인 지수 분포는 TBI 1-8, BMWP 1-93 그리고 CBI는 9-387의 분포를 보였다. 비지도 학습법인 코호넨 신경망을 통하여 지점별 저서생물 출현의 유형화가 잘 반영되어 표출되었다. 또한 하천의 지속적인 관리를 위해 생물학적 지수를 회귀신경망을 통하여 예측하였는데 전체적으로 각 지수의 예측은 실제치와 잘 일치하여 나타났다(TBI, BMWP와 CBI의 상관계수(correlation coefficient)는 각각 0.957, 0.979와 0.967).

• 한국패류학회지
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• 제2권1호
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• pp.13-25
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• 1986

One of the most medically important snail species of Korea is Semisulcospira spp., a member of the freshwater prosobranch family pleuroceridae. The parasites that these snails transmit to humans are mainly Paragonimus westermani and Metagonimus yokogawoi. On the other hand, Semisulcospira snails are edible as a foodstuff in Korea. The present study was first aimed to detect out residual pesticides in the snails collected at the endemic and non-endemic areas of paragonimiasis in Korea and to make relationship to the detectability of larval trematodes shed from the snails. In addition, water specimens from the habitats where the snails were collected were sampled and analysed to elucidate environmental conditions of the habitats. This malaco-ecological survey was done at 7 endemic and 3 non-endemic areas of paragonimiasis for about more than 2 years, May, 1984 through October, 1986. All the water smaples both from the endemic and non-endemic areas of paragonimiasis showed normal ranges of the criteria of upper freshwater streams, with lower B.O.D. (0.4~2.0 ppm) and higher D.O. values (9.5~11.0 ppm) than those in the large river systems Infection rate of digenetic trematodes in total snails collected was 6.7%, but no Paragonimus cercaria was detected out at all even in the endemic areas of paragonimiasis. The ecological factors affecting Paragonimus larvae in the snails were also discussed. A total of 9 chlorinated hydrocarbon pesticieds was targeted to detect out from the tissues of snails. Out of 9 pesticides, aldrin, beta-BHC, p, p-DDT, dieldrin, DDE, lindane, heptachlor and heptachlor epoxide were eluted from the snails. The highest levels of the pesticides were mostly detected out in the snails collected from the Donghae-Chun area of Haman and the Kangwha area of Kyongki-Do, where the lowest infection rates of trematode cercariae (0~0.5%) were revealed. The possibilities that the residual pesticides in the freshwater animals including Semisulcospira snails might be transferable to humans were also discussed.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.36-36
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• 2017

Out of twenty common protein amino acids, there are many kinds of non protein amino acids (NPAAs) that exist as secondary metabolites and exert ecological functions in plants. Mimosine (Mim), one of those NPAAs derived from L. leucocephala acts as an iron chelator and reversely block mammalian cell cycle at G1/S phases. Cysteine (Cys) is decisive for protein and glutathione that acts as an indispensable sulfur grantor for methionine and many other sulfur-containing secondary products. Cys biosynthesis includes consecutive two steps using two enzymes-serine acetyl transferase (SAT) and O-acetylserine (thiol)lyase (OASTL) and appeared in plant cytosol, chloroplast, and mitochondria. In the first step, the acetylation of the ${\beta}$-hydroxyl of L-serine by acetyl-CoA in the existence of SAT and finally, OASTL triggers ${\alpha}$, ${\beta}$-elimination of acetate from OAS and bind $H_2S$ to catalyze the synthesis of Cys. Mimosine synthase, one of the isozymes of the OASTLs, is able to synthesize Mim with 3-hydroxy-4-pyridone (3H4P) instead of $H_2S$ for Cys in the last step. Thus, the aim of this study was to clone and characterize the cytosolic (Cy) OASTL gene from L. leucocephala, express the recombinant OASTL in Escherichia coli, purify it, do enzyme kinetic analysis, perform docking dynamics simulation analysis between the receptor and the ligands and compare its performance between Cys and Mim synthesis. Cy-OASTL was obtained through both directional degenerate primers corresponding to conserved amino acid region among plant Cys synthase family and the purified protein was 34.3KDa. After cleaving the GST-tag, Cy-OASTL was observed to form mimosine with 3H4P and OAS. The optimum Cys and Mim reaction pH and temperature were 7.5 and $40^{\circ}C$, and 8.0 and $35^{\circ}C$ respectively. Michaelis constant (Km) values of OAS from Cys were higher than the OAS from Mim. Inter fragment interaction energy (IFIE) of substrate OAS-Cy-OASTL complex model showed that Lys, Thr81, Thr77 and Gln150 demonstrated higher attraction force for Cys but 3H4P-mimosine synthase-OAS intermediate complex showed that Gly230, Tyr227, Ala231, Gly228 and Gly232 might provide higher attraction energy for the Mim. It may be concluded that Cy-OASTL demonstrates a dual role in biosynthesis both Cys and Mim and extending the knowledge on the biochemical regulatory mechanism of mimosine and cysteine.

• 한국토양비료학회지
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• 제16권1호
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• pp.14-19
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• 1983

포화투수계수(飽和透水係数) 측정방법(測定方法)을 상호비교(相互比較)하여 투수계수(透水係数)의 대양조사(大量調査)에 적합(摘合)한 실용적(実用的)인 측정방법(測定方法)을 선발(選拔)코자 본량(本良) 사양토(砂壤土)에서 침투계법(浸透計法), 역(逆)오가혈법(穴法) 및 코아시료법(試料法)을 비교(比較)한 결과(結果)는 다음과 같다. 1. 침투계법(浸透計法)과 역(逆)오가혈법간(穴法間)에는 고도(高度)의 유의성(有意性)이 있는 회귀관계(回歸関係)가 성립(成立)되었으나 코아시료법(試料法)은 다른 두 방법(方法)에 대해서 전혀 상관(相関)을 나타내지 않았다. 2. 침투계법(浸透計法)에 의(依)해 측정(測定)된 투수계수(透水係数)는 약(約) 80~100분(分) 경과 후 측정(測定)된 한계침투속도(限界浸透速度)와 동일(同一)하였다. 3. 역(逆)오가혈법(穴法)은 5~6회(回) 반복(反覆) 측정(測定)한 값으로부터 안정(安定)된 투수계수(透水係数)를 얻었다. 4. 코아시료(試料)에 의한 침투계수(透水係数) 측정(測定)은 3시간(時間) 이상(以上) 투수(透水)시킨 뒤 얻은 침투계수(透水係数)가 비교적(比較的) 안정(安定)된 값을 나타내었다. 5. 역(逆)오가혈법(穴法)은 측정방법(測定方法)이 간단하고 실용성(実用性)이 높으며 특(特)히 조립질토양(粗粒質土壤)일수록 신뢰도가 높았다.

• 한국가정과교육학회지
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• 제25권1호
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• pp.1-14
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• 2013

본 연구의 목적은 고등학생의 성장환경에 따라 진로의사결정에 차이가 있는가를 알아보는 것이다. 고등학생 600명을 대상으로 설문 조사를 실시하여 539부를 최종분석에 사용하여 성장환경 변인에 따라 군집분석을 실시하여 2개의 집단으로 구분하였다. 군집 A와 B는 성장환경에서 독립-성취 지향 요인을 제외하고 나머지 하위 요인의 점수에서 유의한 차이가 있어 성장환경의 특성이 서로 다른 집단임을 검증하였다. A group는 B group보다 정치적 사회적 지적 문화적인 활동에 관심을 갖고, 사회적 활동과 여가활동에 적극 참여하며 윤리적 도덕적 종교적 문제와 가치를 강조한다. 진로의사결정의 합리적 유형 점수는 A group보다 B group이 높고, 의존적 유형 점수는 A group이 B group보다 높다. 직업정보수집은 A group이 B group보다 점수가 높고, 목표설정과 자기평가 요인에서는 B group이 A group보다 점수가 높다. 성장환경의 특성에 따라 진로의사결정 유형과 자기효능감 점수는 차이가 있으므로 가정에서는 자녀에게 긍정적인 성장환경을 제공하는 것에 관심을 갖고 다양한 자극과 풍부한 경험이 제공되는 성장환경을 조성해 줌으로써 자녀들이 자신의 적성에 맞는 진로를 탐색하고 결정하는데 도움을 주어야 할 것이다.

• 한국가정과교육학회지
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• 제25권3호
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• pp.61-77
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• 2013

본 연구의 목적은 교과서 평가기준에 반영된 교과 관점의 중요성을 탐색하고, 현재 실과(기술 가정) 교육과정에 제시된 관점과 교과서 평가기준과의 관련성과 더불어 향후 교과서 평가기준을 방향을 설정하는 것이었다. 따라서 본 연구의 내용은 실과(기술 가정) 교과서 평가기준을 분석하고, 교과서 평가기준에서의 교과 관점의 의미를 탐색하며, 2007 개정 교육과정 및 2011 개정 교육과정에 지속적으로 제시된 교과 관점을 도출하고, 교육과정과 교과서 평가기준에서 강조하고 있는 관점의 관련성을 분석하며, 마지막으로 향후 교과서 평가기준 개발 방향을 제시하는 것을 포함하고 있다. 교과서 평가기준은 교과서 개발을 위한 교과관점을 제공하고, 교과의 주요 개념과 가치를 전달하며, 교사교육의 방향을 제시해 줄 수 있다는 점에서 중요한 의미를 가진다. 본 연구에서 교육과정 분석을 통해 도출된 교과 관점들은 관리역량, 생활역량, 실천적 경험, 개인과 가족을 이해하기 위한 통합역량, 생활문제해결, 그리고 진로 및 생애설계이다. 향후 국가 수준의 교육과정에서 제시된 교과 관점들에 터하여 교과서 평가기준의 항목들을 개발할 필요가 있으며, 학습 자료 평가기준뿐만 아니라, 교수 학습 방법, 교사 활동 평가 등 다양한 평가 틀에 교과 관점들이 활용될 수 있을 것으로 기대한다.