• Natural Product Sciences
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• 제27권4호
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• pp.274-279
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• 2021

The therapeutic effects of the leaves of Couroupita guianensis, a large tropical tree in the family of Lecythidaceae improving testosterone-induced Benign Prostatic Hyperplasia (BPH) were tested in vitro and in vivo. In BPH rats induced by castration and testosterone treatment, the prostate index was improved in groups administered with the extracts of C. guianensis extracted with 50%-, 100%-ethanol or boiling water, which was comparable with positive control, finasteride. The extract C. guianensis leaves showed significant inhibition on the expressions of type 2 5-alpha reductase (5αR) in RWPE-1 human prostatic epithelial cells, and effectively attenuated the expressions of androgen receptor, type 2 5αR and proliferating cell nuclear antigen in LNCap human prostatic adenocarcinoma cells. The leaves of C. guianensis that exerted evident suppression on BPH-related biomarkers in vitro and improvement of prostate index in vivo has a potential therapeutic use for the treatment of BPH.

• Journal of Preventive Medicine and Public Health
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• 제55권6호
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• pp.586-594
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• 2022

Objectives: This study investigated the effectiveness of the social marketing mix approach in increasing students' knowledge about smoking, promoting positive attitudes toward smoking cessation, and decreasing smoking behavior. Methods: This quantitative research study incorporated a quasi-experimental method with a pretest-posttest non-equivalent group design. Using the purposive sampling technique, 152 smoking students were selected as participants. They were divided into 2 equal groups, with 76 students in the control group and 76 in the intervention group. The data were collected using questionnaires and analyzed with the chi-square test, independent t-test, Wilcoxon signed-rank test, and Mann-Whitney U-test. Results: The social marketing mix intervention was effective in increasing the students' knowledge about smoking (p<0.001), improving their attitude toward smoking cessation (p<0.001), and reducing their smoking behavior (p=0.014). Conclusions: This approach should be implemented by local governments to reduce smoking behavior in the community, especially among teenagers, in addition to instituting a smoking ban and applying fines.

• Asian Pacific Journal of Cancer Prevention
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• 제15권14호
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• pp.5767-5772
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• 2014

Background and Aim: B7-H1, a co-inhibitory molecule of the B7 family, is found aberrantly expressed in ovarian cancer cells and infiltrating macrophage/dendritic-like cells, and plays a critical role in immune evasion by ovarian cancer. IL-12, an inducer of Th1 cell development, exerts immunomodulatory effects on ovarian cancer. However, whether IL-12 regulates B7-H1 expression in human ovarian cancer associated-macrophages has not been clarified. Therefore, we investigated the effects of IL-12 on the expression of B7-H1 in ovarian cancer-associated macrophages and possible mechanisms. Methods: PMA induced THP-1-derived macrophages or human monocyte-derived macrophages were treated with recombinant IL-12 (rIL-12) or infected with adenovirus carrying human IL-12 gene (Ad-IL-12-GFP) for 24 h, then cocultured with the SKOV3 ovarian cancer cell line for another 24 h. Macrophages were collected for real-time PCR and Western blot to detect the expression of B7-H1, and activation of the NF-${\kappa}B$ signaling pathway. Moreover, supernatants were collected to assay for IL-12, IFN-${\gamma}$ and IL-10 by ELISA. In addition, monocyte-derived macrophages treated with IFN-${\gamma}$ were cocultured with SKOV3 and determined for the expression of B7-H1. Furthermore, the expression of B7-H1 in monocyte-derived macrophages was also evaluated after blocking NF-${\kappa}B$ signaling. Results: The expression of B7-H1 was significantly upregulated in monocyte-derived macrophages treated with rIL-12 or Ad-IL-12-GFP compared with the control groups (p<0.05), accompanied by a remarkable upregulation of IFN-${\gamma}$ (p<0.05), a marked downregulation of IL-10 (p<0.05) and activation of NF-${\kappa}B$ signaling. However, the upregulation of B7-H1 was inhibited by blocking the NF-${\kappa}B$ signaling pathway (p<0.05). Expression of B7-H1 was also increased (p<0.05) in monocyte-derived macrophages treated with IFN-${\gamma}$ and cocultured with SKOV3. By contrast, the expression of B7-H1 in THP-1-derived macrophages was significantly decreased when treated in the same way as monocyte-derived macrophages (p<0.05), and IL-10 was also significantly decreased but IFN-${\gamma}$ was almost absent. Conclusions: IL-12 upregulates the expression of B7-H1 in monocyte-derived macrophages, which is possible though inducing the secretion of IFN-${\gamma}$ and further activating the NF-${\kappa}B$ signal pathway. However, IL-12 downregulates the expression of B7-H1 in THP-1-derived macrophages, associated with a lack of IFN-${\gamma}$ and inhibition of expression of IL-10.

• 한국노년학
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• 제38권1호
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• pp.243-259
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• 2018

본 연구는 노인의 일상생활수행능력(ADL)과 도구적 일상생활수행능력(IADL)이 우울감에 미치는 영향에 대한 지역사회 재가노인복지시설의 조절효과를 탐색하는 데에 그 목적이 있다. 이를 위해 고령화연구패널 5차(2014년)자료와 지역사회 재가노인복지시설 수에 대한 보건복지부 현황 자료(2014년)를 결합하여 다층분석을 실시하였다. 연구대상은 65세 이상 노인 4,139명이다. 종속변수는 우울감이며, 개인수준 독립변수는 ADL과 IADL이다. 지역수준의 조절변수는 시 도의 노인인구 1만 명당 재가노인복지시설의 수(재가노인복지시시설 비율)이다. 연구결과, ADL이 우울에 미치는 영향은 재가노인복지시설의 비율에 따라서 달라진다. 즉, 노인인구 1만 명당 재가노인복지시설의 수가 많은 지역에 거주하는 노인일수록 ADL이 우울에 미치는 영향력이 작은 것으로 나타났다. IADL에 관해서는 제한 수준이 높을수록 노인이 더 우울해지는 경향이 있었으나 IADL과 재가노인복지시설 비율의 상호작용 효과는 발견되지 않았다. 본 연구결과는 일상생활수행과 관련된 개입 서비스를 지역사회 재가노인복지시설을 통해 전달하는 것이 노인의 우울감 완화에 효과적일 수 있음을 시사한다. 특히 재가노인복지시설이 지역사회 노인 수요에 맞추어 충분히 제공될 때 복지제도의 효율성이 높아질 수 있음을 뒷받침한다.

• 호스피스학술지
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• 제1권1호
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• pp.46-69
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• 2001

Hospice Care is the best way to care for terminally ill patients and their family members. However most of them can not receive the appropriate hospice service because the Korean health delivery system is mainly be focussed on acutly ill patients. This study was carried out to clarify the situation of hospice in Korea and to develop a hospice care delivery system model which is appropriate in the Korean context. The theoretical framework of this study that hospice care delivery system is composed of hospice resources with personnel, facilities, etc., government and non-government hospice organization, hospice finances, hospice management and hospice delivery, was taken from the Health Delivery System of WHO(1984). Data was obtained through data analysis of litreature, interview, questionairs, visiting and Delphi Technique, from October 1998 to April 1999 involving 56 hospices, 1 hospice research center, 3 non-government hospice organizations, 20 experts who have had hospice experience for more than 3 years(mean is 9 years and 5 months) and officials or members of 3 non-government hospice organizations. There are 61 hospices in Korea. Even though hospice personnel have tried to study and to provide qualified hospice serices, there is nor any formal hospice linkage or network in Korea. This is the result of this survey made to clarify the situation of Korean hospice. Results of the study by Delphi Technique were as follows: 1.Hospice Resources: Key hospice personnel were found to be hospice coordinator, doctor, nurse, clergy, social worker, volunteers. Necessary qualifications for all personnel was that they conditions were resulted as have good health, receive hospice education and have communication skills. Education for hospice personnel is divided into (i)basic training and (ii)special education, e.g. palliative medicine course for hospice specialist or palliative care course in master degree for hospice nurse specialist. Hospice facilities could be developed by adding a living room, a space for family members, a prayer room, a church, an interview room, a kitchen, a dining room, a bath facility, a hall for music, art or work therapy, volunteers' room, garden, etc. to hospital facilities. 2.Hospice Organization: Whilst there are three non-government hospice organizations active at present, in the near future an hospice officer in the Health&Welfare Ministry plus a government Hospice body are necessary. However a non-government council to further integrate hospice development is also strongly recommended. 3.Hospice Finances: A New insurance standards, I.e. the charge for hospice care services, public information and tax reduction for donations were found suggested as methods to rise the hospice budget. 4.Hospice Management: Two divisions of hospice management/care were considered to be necessary in future. The role of the hospice officer in the Health & Welfare Ministry would be quality control of hospice teams and facilities involved/associated with hospice insurance standards. New non-government integrating councils role supporting the development of hospice care, not insurance covered. 5.Hospice delivery: Linkage&networking between hospice facilities and first, second, third level medical institutions are needed in order to provide varied and continous hospice care. Hospice Acts need to be established within the limits of medical law with regards to standards for professional staff members, educational programs, etc. The results of this study could be utilizes towards the development to two hospice care delivery system models, A and B. Model A is based on the hospital, especially the hospice unit, because in this setting is more easily available the new medical insurance for hospice care. Therefore a hospice team is organized in the hospital and may operate in the hospice unit and in the home hospice care service. After Model A is set up and operating, Model B will be the next stage, in which medical insurance cover will be extended to home hospice care service. This model(B) is also based on the hospital, but the focus of the hospital hospice unit will be moved to home hospice care which is connected by local physicians, national public health centers, community parties as like churches or volunteer groups. Model B will contribute to the care of terminally ill patients and their family members and also assist hospital administrators in cost-effectiveness.

• 한국산림과학회지
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• 제109권4호
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• pp.371-381
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• 2020

환경부는 2020년 야생생물 보호 및 관리에 관한 법률 시행령을 통해 멸종위기 야생생물 지정은 세계자연보전연맹(IUCN)이 제시한 지역적색 목록 지침을 준용하기로 정하였고, 적색목록 관속식물 377종을 지정하였다. 본 연구는 IUCN의 지역 적색 평가를 근간으로 평가불가(NA)를 제외한 103종을 선별하고, 국제적 수준의 적색목록 목록평가 가능한 고유종 10종을 제외한 이후 93종에 대한 재평가를 실시하였다. 본 분석에서 적용 불가 34종이 추가로 확인되어 59종에 대한 지역적색 평가를 적용하였다. 재평가 결과 취약이상의 분류군은 16종으로 CR(위급) 1종, EN(위기) 10종, VU(취약) 5종으로 평가되었고, NT(준위협) 4종과 LC(약관심) 30종, DD(정보부족) 9종을 판정하였다. 환경부 지역적색목록의 평가기준 B의 경우 정량적 분포자료나 지속적인 감속에 대한 자료없이 평가를 시도하여 데이터의 신뢰도가 없었으며 난과에 속한 멸종위기식물은 판정시 필요한 남획의 구체적인 증거나 자료확보가 미비하다. 현재 환경부의 적색목록에 대한 문제점은 객관적 기초 자료가 부재하고 중장기적인 개체군 크기의 증감에 대한 모니터링 활동이 없어 과학적 평가를 어렵게 하고 있다. 환경부는 법적으로 범주 및 평가에 대한 것을 지정하고 그 기준을 스스로 따르지 못하는 전문성의 결여가 큰 문제로 인식되며, 또한 종의 분포 및 상태에 대한 지리적 및 분류학적 편향성은 자료의 질과 양에 큰 영향을 주고 있다. 이런 문제를 극복하고 생물다양성 정책의 발전을 위해서는 객관적이고 합리적인 멸종위기종 생물 자료 관리가 필요하다.

• 벤처창업연구
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• 제9권6호
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• pp.109-115
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• 2014

중소벤처기업으로 시작한 온라인 게임 회사들의 국내 시장규모가 10조 원을 상회할 정도로 급성장하면서, 게임산업이 산업정책과 규제의 대상화되는 문제에 대해 관심이 집중되고 있다. 특히, 웹보드게임 서비스와 관련 규제를 강화해 온 정부는 영상물등급위, 게임물 관리위원회의 모바일 웹보드게임에 대한 행정처분, 문화체육관광부의 게임산업진흥법 시행령, 여성가족부가 주도한 청소년보호법 상의 셧다운제 등 여러 주무 부서를 통해 규제를 시행 중이다. 본 연구는 2013년 7월 이후 1년여 간의 게임트릭스 시계열 자료를 이용하여 2013년 이후 강화된 게임규제의 효과를 벡터오차수정 모형(VECM, Vector Error Correction Model)을 활용하여 실증 분석하였다. 이 과정에서 추정된 공적분 관계식을 통해 웹보드게임 업계의 장기 균형관계를 분석하는 한편, 오차수정모형의 추정 분석을 통해 웹보드게임 간 발생하는 충격의 파급효과와 예측 이용시간을 분석하였다. 분석 결과, 게임 규제가 강화된 2013년 이후 대표적인 웹보드게임의 이용시간은 2013년 시작점 대비, 1/3 ~ 1/4 수준으로 줄어든 것으로 예측되었다. 즉, 대표 웹보드게임 2종의 이용은 현저히 줄어들거나 이용되지 않을 가능성에 놓여있는 것이다. 추가적으로 웹보드게임의 게임산업 전반의 영향에 대한 논점을 제시하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제30권4호
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• pp.462-469
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• 2017

Objective: The aim of this study is to identify genomic regions or genes controlling growth traits in pigs. Methods: Using a panel of 54,148 single nucleotide polymorphisms (SNPs), we performed a genome-wide Association (GWA) study in 562 pure Yorshire pigs with four growth traits: average daily gain from 30 kg to 100 kg or 115 kg, and days to 100 kg or 115 kg. Fixed and random model Circulating Probability Unification method was used to identify the associations between 54,148 SNPs and these four traits. SNP annotations were performed through the Sus scrofa data set from Ensembl. Bioinformatics analysis, including gene ontology analysis, pathway analysis and network analysis, was used to identify the candidate genes. Results: We detected 6 significant and 12 suggestive SNPs, and identified 9 candidate genes in close proximity to them (suppressor of glucose by autophagy [SOGA1], R-Spondin 2 [RSPO2], mitogen activated protein kinase kinase 6 [MAP2K6], phospholipase C beta 1 [PLCB1], rho GTPASE activating protein 24 [ARHGAP24], cytoplasmic polyadenylation element binding protein 4 [CPEB4], GLI family zinc finger 2 [GLI2], neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adaptor 2 [NYAP2], and zinc finger protein multitype 2 [ZFPM2]). Gene ontology analysis and literature mining indicated that the candidate genes are involved in bone, muscle, fat, and lung development. Pathway analysis revealed that PLCB1 and MAP2K6 participate in the gonadotropin signaling pathway and suggests that these two genes contribute to growth at the onset of puberty. Conclusion: Our results provide new clues for understanding the genetic mechanisms underlying growth traits, and may help improve these traits in future breeding programs.

• 대한건축학회논문집:계획계
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• 제35권7호
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• pp.109-117
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• 2019

Recently, child safety map making education has attracted attention as a way to reduce crimes against children. In Korea, the Ministry of Gender Equality and Family organized a child safety map making education program in 2011. The program's manual was revised in 2013 and the Ministry implemented it as a project to promote the rights of women and children. Child safety map making education aims to raise a child's understanding of their neighborhood, to have voluntary control and normal consciousness as a local inhabitant, to be aware of wrong behavior and crime, and to be part of creating a safe urban environment. However, when compared to educational programs in other major developed countries, the child safety map making education program in Korea currently does not improve a child's awareness of their surroundings. In this workshop study, we proposed and ran a new program to improve children's awareness of their environment based on the active participation of children in the existing safety map educational program. The workshop was held for 4 weeks for 48 students from 5th and 6th grade at Osan Daeho Elementary School. We analyzed this new program's effects with the following results. First, an analysis of the effects of the program on children's recognition of safe and dangerous spaces revealed that their understanding of these spaces increased by 30.4% after the workshop. The safety-related factor in the mind map key concept increased from 0.94 to 4.94, indicating that the children's perception of neighborhood risk and safety factors improved. Second, the analysis of the effects of the program on the children's coping ability in dangerous situations showed that their understanding of how to deal with dangerous situations increased by 11.3%. The children's understanding of facilities they could ask for help, such as police boxes and child safety guard houses, improved by 17.9%. Third, analysis of the effects of child safety map making education on children's understanding of their neighborhood, their perception of responsibility in the neighborhood, and their neighborhood attachment showed that these levels of children's understanding of the neighborhood improved by 6.0% after the workshop.

• 한국방재안전학회논문집
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• 제15권3호
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• pp.57-66
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• 2022

고용노동부 공문(최근 건설업 사망사고 발생 현황 안내 및 안전관리 철저 당부, 2022.4.4.)에 따르면, 중대재해처벌법 시행(2022.1.27.) 이후 2022.3.31.까지 공사금액 50억원 이상 건설공사에서 17건의 사망사고가 발생하여 18명이 사망했다. 공사규모로 보면 50억~120억원 7건, 120억~800억원 5건, 800억원이상 5건이 발생했다. 사망사고 발생원인은 떨어짐 9건(50%), 맞음 5건(28%), 무너짐 2건(11%), 끼임 1건(5%), 익사 1건(5%)으로 조사됐다. 사망사고의 대부분을 차지하는 떨어짐과 맞음으로 인한 사망사고를 줄이기 위해서는 안전시설물을 강화할 필요가 있으며, 추락방지망·개구부·안전난간이 완벽하게 설치되면 대부분의 떨어짐과 맞음 사고는 예방 할 수 있다. 따라서 본 고에서는 소규모 공동주택인 도시형생활주택신축현장의 떨어짐과 맞음 사고를 예방하기 위해서 안전시설물 개선을 통한 특허기술 개발과 활용 사례를 제시하고자 한다.