• Proceedings of the KAIS Fall Conference
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• 2003.06a
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• pp.320-321
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• 2003

최근 천연물을 중심으로 한 학문이 발전하면서 천연물이 가지는 생리활성 물질에 대한 관심이 중대되고 있다. 또한 인공합성품의 일부가 안정성의 문제가 제기되면서 천연물의 이용분야는 더욱 확대되고 있다. 급속한 경제발전과 생활수준의 향상으로 식생활을 포함한 생활방식의 다양화로 인하여 과거 감염 위주의 질병이 감소하고 선진국형의 만성퇴행성 질환이 증가하는 추세이다. 혈소판은 혈전증(thrombosis)과 지혈증(haemstasis)에서 중요한 역할을 담당하고 있는 인자로서 현관 내 병적 이상으로 인한 과도한 혈전의 생성은 뇌 -심혈관계 질환의 중요한 유발인자로 작용하므로 뇌ㆍ심혈관계 질환이나 항고지혈증을 연구하는데 유용하게 이용되고 있다. 본 연구에서는 토끼 혈액에서 분리한 세정 혈소판 부유액을 이용하여 작약 MeOH 추출물에서 분리한 천연생리활성물질들을 대상으로 혈소판 응집억제활성에 대하여 연구하였다. 작약 MeOH 추출물의 혈소판 응집억제활성 측정에서 강한 혈소판 응집억제활성 작용을 보였다. 따라서 작약 MeOH 추출물을 크로마토그라피법을 이용하여 분리하였고, NMR을 이용한 분광학적 방법으로 지금까지의 분리한 자료와 비교ㆍ분석하였다. Monoterpene glycoside 계열의 성분들인 15개의 compound와 다수의 fraction들을 HPLC를 이용하여 분리하였으며, collagen으로 유도된 혈소판 응집억제활성측정 방법에서 뛰어난 응집억제활성을 보였다. 표준물질을 이용한 HPLC 분석과 ¹H-NMR 관련 자료의 검색을 통하여 최종적으로 compound 1b가 benzoyloxy-paeoniflorin(2.6％). compound 1d가 paeond(1.3％), compound 2c가 albiflorin(3.2％), compound 2e가 paeoniflorin(33.6％)임을 확인할 수 있었다. Compound 3a의 분석결과 benzoyloxypaeoniflorin과 구조적 유사성은 있으나 동일한 구조식으로 확인할 수 없었다. 그러나 collagen에서 응집억제활성이 90％ 이상으로 뛰어난 활성을 나타내므로 benzoyloxypaeoniflorin과 유사한 구조에서 benzoyl group이 다른 작용기로 치환되었거나 R₁ group이 다른 작용기로 치환된 형태로 추측하였다. Benzoyloxypaeoniflorin은 collagen＞thrombin＞U46616＞A.A(arachidonic acid)＞PAF의 순으로 활성을 보였다. 이는 paeoniflorin의 glycoside 5-carbon위치에 위치한 OH기 대신에 benzoyl기로 치환된 benzoyl 기가 혈소판 억제 산물로 작용한 것으로 추측했다. Paeoniflorin.은 U46619＞thrombin＞collagen＞A.A＞PAF순으로 억제를 보였다. Paeoniflorin이 collagen보다 thrombin에서 강한 억제를 보이는 것으로 Ca/sup 2＋/ chelate를 형성함으로 인해 calciu 대사를 저해하는 것으로 추축했다. Compound 3a는 U46619＞collagen＞A.A＞thrombin＞PAF순으로 억제율을 보이므로 이 화합물은 paeoniflorin의 benzoyl기에 OH기가 다른 치환기로 바뀌거나 paeoniflorin의 glycoside 5-carbon 위치의 OH기 대신에 다른 작용기로 치환된 것으로 추정하였다. 이러한 결과로 작약의 주성분인 paeoniflorin과 유사한 구조를 가진 다른 monoterpene glycoside 계열의 화합물들과 비교 분석하고 구조를 화인하고 이들 성분이 어떻게 혈소판 응집억제활성에 작용하는지를 연구하였다.

• Journal of Korean Society of Forest Science
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• v.102 no.3
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• pp.309-314
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• 2013

The concentration of terpene (18 species) was measured from spring to fall in ambient air and essential oil at three different forests located in Jeollanamdo province. Sampling was conducted at 4-hour-interval a day of each season. In the ambient air, ${\alpha}$-pinene, ${\beta}$-pinene and 2-carene were the most abundant compounds throughout the sampling periods and areas. The highest three-season average concentration of total terpene was 2,597 pptv at Jeamsan(Mt.) in Boseong which was predominantly populated by Pinaceae. The seasonal averages were observed to peak during spring with their max at Palyeongsan(Mt.) in Goheung and Jeamsan(Mt.) in Boseong, and during fall at Woodland in Jangheung. Most of terpenes had diurnal variations with higher concentrations during the daytime, and lower during the nighttime. However in essential oil, the highest annual average concentration of total terpene was $798{\mu}g/dry-g$ at Goheung which was predominantly populated by Chamaecyparis obtusa. Also, the component proportion ratio showed different distribution between ambient air and essential oil. From the results of this study, we suggest that Chamaecyparis obtusa species is more useful, and younger tree is more economical than older one, and spring is the best season for lumbering in order to use essential oil.

• Korean Journal of Food Science and Technology
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• v.50 no.1
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• pp.44-54
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• 2018

To maintain the best quality of kimchi during long-term storage, we developed a fermentation storage mode for the kimchi refrigerator. The optimal kimchi fermentation temperature was determined to be $6^{\circ}C$ with fermentation time of 4-7 days in winter and 3-5 days in spring and fall. Based on these results, the fermentation storage mode conditions were programmed to consist of a fermentation temperature of $6^{\circ}C$ and fermentation times of 111 h in winter and 58 h in spring/fall. When kimchi was stored under the developed fermentation storage mode conditions, the total acidity of kimchi was almost the same as that of the control kimchi (stored $-2-\;-1^{\circ}C$ for 12 weeks). However, the number of lactic acid bacteria (LAB) and Leuconostoc sp. in kimchi were higher ($10^1-10^2CFU/mL$) than those in the control kimchi during storage. In addition, kimchi fermented and stored under the fermentation storage mode clearly received higher scores for overall preference than the control kimchi.

• Economic and Environmental Geology
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• v.41 no.1
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• pp.15-32
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• 2008

The hydrochemical and isotopic (stable isotopes and noble gas isotopes) analyses for hot spring waters, cold groundwaters and surface water samples from the Seokmodo hot spring area of the Ganghwa province were carried out to characterize the hydrogeochemical characteristics of thermal waters and to interpret the source of thermal water and noble gases and the geochemical evolution of hot spring waters in the Seokmodo geothermal system. The hot spring waters and groundwaters show a weakly acidic condition with the pH values ranging from 6.42 to 6.77 and 6.01 to 7.71 respectively. The outflow temperature of the Seokmodo hot spring waters ranges from $43.3^{\circ}C\;to\;68.6^{\circ}C$. Relatively high values of the electrical conductivities which fall between 60,200 and $84,300{\mu}S/cm$ indicate that the hot spring waters were mixed with seawater in the subsurface geothermal system. The chemical compositions of the Seokmodo hot spring waters are characterized by Na-Ca-Cl water type. On the other hand, cold groundwaters and surface waters can be grouped into three types such as the Na(Ca)-$HCO_3$, Na(Ca)-$SO_4$ and Ca-$HCO_3$ types. The ${\delta}^{18}O\;and\;{\delta}D$ values of hot spring waters vary from -4.41 to -4.47%o and -32.0 to -33.5%o, respectively. Cold groundwaters range from -7.07 to -8.55%o in ${\delta}^{18}O$ and from -50.24 to -59.6%o in ${\delta}D$. The oxygen and hydrogen isotopic data indicate that the hot spring waters were originated from the local meteoric water source. The enrichments of heavy isotopes ($^{18}O\;and\;^2H$) in the Seokmodo hot spring waters imply that the thermal water was derived from the diffusion Bone between fresh and salt waters. The ${\delta}^{34}S$ values ranging from 23.1 to 23.5%o of dissolved sulfate are very close to the value of sea water sulfate of ${\delta}^{34}$S=20.2%o in this area, indicating the origin of sulfate in hot springs from sea water. The $^3H/^4He$ ratio of hot spring waters varies from $1.243{\times}10^{-6}\;to\;1.299{\times}10^{-6}cm^3STP/g$, which suggests that He gas in hot spring waters was partly originated from a mantle source. Argon isotopic ratio $(^{40}Ar/^{36}Ar=298{\times}10^{-6}cm^3STP/g)$ in hot spring waters corresponds to the atmospheric value.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.1
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• pp.1-6
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• 2008

Korditwo is a new orchardgrass(Dactylis glomerata L.) variety developed by the National Institute of Animal Science(NIAS) in 2005. To develope the new variety of orchardgrass, 5 superior clones, Dg8533, Dg9518, EDg9133, U8504 and U8519, were selected and polycrossed for seed production. That synthetic variety was designated newly as "Korditwo", and it's agronomic growth characteristics and forage productivity were examined at Suwon from 2001 to 2002, and regional yield trials were conducted in Suwon, Pyungchang, Youngju, Iksan, and Jeju from 2003 to 2005, respectively. "Korditwo" showed medium type growth habit in fall and spring and short in length of flag leaf and upper internode. Plant height of "Korditwo" was similar to that of standard variety, "Ambassador" and heading date was 4 days later than 14th May compared to Ambassador. Characteristics such as winter hardiness, lodging tolerance, disease resistance of "Korditwo" were stronger or better than those of Ambassador, specially in winter hardiness and lodging tolerance. "Korditwo" showed 10% higher dry matter yield(11.3 ton/ha) compared to Amabassador. Nutritive value was appeared to be similar in both varieties.

• Proceedings of the KAIS Fall Conference
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• 2010.11b
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• pp.915-918
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• 2010

12주간의 유산소 운동 및 복합성 운동이 중년여성의 신체 구성 및 혈중 지질에 미치는 영향을 규명하기 위해 2009년 3월부터 9월까지 일개 D광역시 소재 일개 S구 보건소 12주 운동프로그램에 참가한 40~50대 중년 여성 78명을 대상으로 비만그룹과 정상그룹으로 나누어 주3회, 회당 80분의 유산소 운동과 유산소운동/근력 운동을 병행한 복합성 운동을 목표 심박수 40~60%에서 실시하였다. 1. WHO 기준 적용 시, 정상군의 경우 운동의 유형에 상관없이 유의한 차이가 나타났다. 2. WHO 기준 적용 시, 비만군의 경우 유산소 운동의 근육량, 체지방량, 체지방률을 제외하고 운동의 유형에 상관없이 유의한 차이가 나타났다. 3. 아시아-태평양 기준 적용 시, 정상군의 경우 일부 항목에서만 유의한 차이가 나타났다. 4. 아시아-태평양 기준 적용 시, 비만군의 경우 유산소 운동의 근육량을 제외하고 운동의 유형에 상관 없이 유의한 차이가 나타났다. 상기 결론에서 볼 수 있듯이, 12주간의 운동 프로그램은 WHO 기준 적용 시 일부 항목을 제외하고 운동 유형에 상관없이 40~50대 중년여성의 신체조성 및 혈중지질에 긍정적 효과를 미치는 것으로 판단된다. 그러나 비만에 대해 더 엄격한 아시아 - 태평양 기준 적용 시 특히 정상 군에서 유의한 차이를 보이지 않는 항목이 많이 나타났는데 이것은 우리나라 비만기준을 아시아태평양기준에서 세계 기준으로 바꿀 필요가 있다는 주장을 뒷받침하고 있다. 세계보건기구 WHO는 2004년에 이미 우리나라에 세계비만기준을 따를 것을 권고했는데, 실제 위험성이 정확하게 평가돼야지 정확한 대책이나 관리가 나올 수 있기 때문이다. WHO 기준에 의거 시 질병의 발생 위험도가 낮은 경도비만이 엄격한 아시아-태평양 지역 기준 적용 시 비만으로 분류되어 불필요하게 체형에 대한 부정적인 이미지나 스트레스를 받을 가능성도 있다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.62-62
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• 2020

Ginseng is a shade-plant cultivated using shading facilities. However, at too low light levels, root growth is poor, and at high light levels, the destruction of chlorophyll reduces the photosynthesis efficiency due to leaf burn and early fall leaves. The ginseng has a lightsaturation point of 12,000~15,000 lux when grown at 15 to 20℃ and 9,500 lux at 25℃. This study was conducted to select the optimal light intensity of 3-year-old ginseng grown in blue-white film plastic house. The seeds were planted in the blue-white film plastic house with different light receiving rate (March 17, 2020). Between April and September, the average air temperature in the house was 20.4-20.7℃. Average soil temperature was 18.3℃-18.5℃. The chemical properties of the test soil was as follows. The pH level was 7.0-7.4, EC was 0.5-0.6 dS/m, OM was at the levels of 33.6-37.7 g/kg, P₂O₅ was 513.0-590.8 mg/kg, slightly higher than the allowable 400 mg/kg. The amount of light intensity, illuminance, and solar radiation in the blue-white film house was increased as the light-receiving rate increased and the amount of light intensity was found to be 9-14% compared to the open field, 8-13% illuminance and 9-14% solar irradiation respectively. The photosynthesis rate was the lowest at 3.1 µmolCO²/m²/s in the 9% light blue-white plastic house and 4.2 and 4.0 µmolCO²/m²/s in the 12% and 14% light blue-white plastic house, respectively. These results generally indicate that the photosynthesis of plants increases with the amount of light, but the ginseng has a lower light saturation point at high temperatures, and the higher the amount of light, the lower the photosynthetic efficiency. The SPAD (chlorophyll content) value decreased as the increase of light-receiving rate, and was the highest at 32.7 in 9% light blue-white plastic house. Ginseng germination started on April 11 and took 13-15 days to germinate. The overall germination rate was 82.9-85.8%. The plant height and length of stem were long in the 9% light-receiving plastic house. The diameter of stem was thick in the 12-14% light-receiving plastic house. In the 12% and 14% light-receiving plastic house, the length and diameter of taproot was long and thick, so the fresh weight of root per plant was 20 g or more, which was heavier than 16.9 g of the 9% light-receiving plastic house. The disease incidence (Alternaria blight, Gray mold and Damping-off etc.) rate were 0.9-2.7%. The incidence of Sclerotinia rot disease was 7.5-8.4%, and root rot was 0-20.0%. The incidence ratio of rusty root ginseng was 34.4-38.7% level, which was an increase from the previous year's 15% level.

• Journal of Mushroom
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• v.6 no.1
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• pp.13-19
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• 2008

This experiment was carried out to examine physiological and cultural characteristics of two strains ASI 19003, 'Poplar field-cap mushroom' Agrocybe cylindracea, and ASI 19016, 'Chaxingo' A. chaxingu, at the bottle cultivation which have very similar morphological characteristics in genus Agrocybe. There was significant difference between the physiological and cultural characteristics of ASI19003 and ASI19016. The optimal temperature for the hyphal growth was $28^{\circ}C$ in the strain ASI19003 and $30^{\circ}C$ in ASI19016. The optimal pH was not different in two strains and these strains grew well at pH 5.5~7.0. But the optimal pH in the submerged culture was 5.5 in ASI19003 and 5.0 in ASI19016. Especially, hyphal growth of the strain ASI19016 was very poor at pH 6.0~7.3. The optimal carbon source for the growth was lactose in the strain ASI19003 and fructose in ASI19016, and nitrate sources were asparagine, alanine, and glycine in the strain ASI19003, and ammonium tartrate, asparagine, glycine, and alanine in ASI19016, respectively. The periods of incubation and fruiting body formation in the bottle cultivation during the spring were 27 and 13 days in the strain ASI19003, 29 and 17 days in ASI19016. The yields of fruit body were 114 g per bottle (850 $m{\ell}$ volume) in the strain ASI19003 and 100 g in ASI19016. In the summer, the periods of hyphal incubation and fruiting body formation were 29 and 11 days in the strain ASI19003, 30 and 12 days in ASI19016. The color of the cap in the ASI19003 strain according to temperature increase during the fruit body development become more pale, but the strain ASI19016 kept dark color relative to ASI19003. The fruiting body formation of the strain ASI19016 was faster than that of ASI19003. Accordingly, the cultivation of A. cylindracea ASI19003 during the spring, fall and winter, and A. chaxingu ASI19016 during the summer can keep high quality and stable supply all year round of these mushrooms.

• Korean Journal of Environmental Biology
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• v.22 no.2
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• pp.247-258
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• 2004

Climatological, hydrological and planktonical research studies, measurements of primary production and photosynthetic efficiency from December 1976 to December 1978 have been carried out in two brackish lakes: Lake Etang de Berre and Lake Etang de Vaine located in the French Mediterranean coast, in the region of Carry-le-Rouet located on the north-west Mediterranean near Marseilles, and in fresh water inflows from 4 Rivers (Touloubre, Durance, Arc, Durancole) to Lake Etang de Berre. Physico-chemical parameters were measured for this study: water temperature, salinity, density, pH, alcalinity, dissolved oxygen (% saturation), phosphate, nitrate, nitrite, silicate etc. Diverse biological parameters were also studied: photosynthetic pigments, phaeopigments, specific composition and biomass of phytoplankton, primary pelagic production etc. Climatical factors were studied: air-temperature, solar-radiation, evaporation, direction (including strength) of winds, precipitation and freshwater volume of the four rivers. The changes in Lake ‘Etang de Berre’ ecosystem depend on the quality of the water in the Durance River, and on the effects of seawater near the entrance of the Caronte Canal. The water quality of the lake varies horizontally and vertically as a result of atmospheric phenomena, maritime currents and tides. The distribution of water temperatures is generally heterogeneous. Southeasterly winds and the Northeasterly Mistral wind are important in the origins of circulated and mixed water masses. These winds are both frequent and strong. They have, as a result, a great effect on the water environment of Lake Etang de Berre. In theory, the annual precipitation in this region is well over eight times the water mass of the lake. The water of the Durance River flows into Lake Etang de Berre through the EDF Canal, amounting to 90% of the precipitation. However, reduction of rainfall in dry seasons has a serious effect on the hydrological characteristics of the lake. The temperature in the winter is partially caused by the low temperature of fresh water, particularly that of the Durance River. The hydrological season of fresh and brackish water is about one month ahead of the hydrological season of sea water in its vicinity. The salinity of Lake Etang de Berre runs approximately 3$\textperthousand$, except at lower levels and near the entrance to the Caronte Canal. However, when the volume of the Durance River water is reduced in the summer and fall, the salinity rises to 15$\textperthousand$. In the lake, the ratio of fresh water to sea water is six to one (6:1). The large quantities of seston conveyed by rivers, particularly the Durance diversion, strongly reduce the transparency in the brackish waters. Although the amount of sunshine is also notable, transparency is slight because of the large amount of seston, carried chiefly by Tripton in the fresh water of the Durance River. Therefore, photosynthesis generally occurs only in the surface layer. The transparency progressively increases from freshwater to open seawater, as mineral particles sink to the bottom (about 1.7kg $m^{-2}a^{-1}$ on the average in brackish lakes). The concentration of dissolved oxygen and the rate of oxygen saturation in seawater (Carry-le-Rouet) ranged from 5.0 to 6.0 $m\ell$ㆍ.$1^{-1}$, and from 95 to 105%, respectively. The amount of dissolved oxygen in Etang de Berre oscillated between 2.9 and 268.3%. The monographs of phosphate, nitrate, nitrite and silicate were published as a part of a study on the ecology of phytoplankton in these environments. Horizontal and vertical distributions of these nutriments were studied in detail. The recent diversion of the Durance River into Lake Etang de Berre has effected a fundamental change in this formerly marine environment, which has had a great impact in its plankton populations. A total of 182 taxa were identified, including 111 Bacillariophyceae, 44 Chlorophyceae, and 15 Cyanophyceae. The most abundant species are small freshwater algae, mainly Chlorophyceae. The average density is about $10^{8}$ cells $1^{-1}$ in Lake Etang de Berre, and about double that amount in Lake Etang de Vaine. Differences in phytoplankton abundance and composition at the various stations or at various depths are slight. Cell biovolume V (equivalent to true biomass), plasma volume VP (‘useful’ biomass) and, simultaneously. the cell surface area S and S/V ratio through the measurement of cell dimensions were computed as the parameters of phytoplankton productivity and metabolism. Pigment concentrations are generally very high on account of phytoplankton blooms by Cyanophyceae, Chlorophyceae and Cryptophyceae. On the other hand, in freshwaters and marine waters, pigment concentrations are comparatively low and stable, showing slight annual variation. The variations of ATP concentration were closely related to those of chlorophyll a and phytoplankton blooms only in marine waters. The carbon uptake rates ranged between 38 and 1091 mg$Cm^{-2}d^{-1}$, with an average surface value of 256 mg; water-column carbon-uptake rates ranged between 240 and 2310 mg$Cm^{-2}d^{-1}$, with an average of 810, representing 290 mg$Cm^{-2}$, per year 45 000 tons per year of photosynthetized carbon for the whole lake. Gross photosynthetic production measured by the method of Ryther was studied over a 2-year period. The values obtained from marine water(Carry-le-Rouet) ranged from 23 to 2 337 mg$Cm^{-2}d^{-1}$, with a weighted average of 319, representing about 110 gCm$^{-2}$ per year. The values in brakish water (Etang de Berre) ranged from 14 to 1778 mg$Cm^{-2}d^{-1}$, with a weighted average of 682, representing 250 mg$Cm^{-2}$ per year and 38 400 tons per year of photosynthesized carbon for the whole lake.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.4
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• pp.668-678
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• 2004

The purpose of this study was to estimate seasonal variation of nutritional intake and quality in adults in longevity areas. Dietary survey was given to 469 subjects over age 20 living Bukjeju-gun, Yecheon-gun, and Sunchang-gun of Korea, using 24-hour recall method every 4 seasons over one-year period. The mean daily intakes (%RDA) of 4 seasons were 1313.3 ㎉ (72.0%) for energy, 47.3 g (82.2%) for protein, 20.2 g for fat, 228.0 g for carbohydrate, 12.8 g for dietary fiber. The differences were hardly significant among the seasons. Mean daily intakes of most of the vitamins and minerals were lower than RDA except vitamin C and folate, especially %RDAs of Ca, vitamin D, vitamin B$_2$, vitamin E were less than 60% of RDA. In general, nutrient intake were high in spring compared to other seasons except vitamin C which was high in fall and winter. Mean daily intakes of cholesterol were 151.7 mg and 124.3 mg in males and females, respectively. The differences was significant between the two sexes but not among the seasons. PUFA : MUFA : SFA ratio of the subject was 1.0 : 1.3 : 1.2. Average CPF ratio of energy intake was 72.7 : 14.4 : 12.9, and energy intake ratio from carbohydrate was low in spring, in contrast energy intake ratios from protein was significantly high in spring. Mean adequacy ratio (MAR), an index of overall nutritional quality was 0.64 for female and 0.71 for male. The indices of nutritional quality (INQ) were over 1 for most of nutrients except 0.73 of Ca, 0.87 of vitamin A, 0.69 of vitamin B$_2$, and 0.65 of vitamin E. Both MAR and INQ were significantly different among sex and seasons, values were higher in males than in females and were higher in spring with the exception of vitamin C. In conclusion, subjects in longevity areas did not consume enough nutrients quantitatively as well as qualitatively, especially Ca, vitamin A, vitamin B$_2$, and vitamin E. Also mean daily intakes of most of vitamins and minerals were insufficient in females, and were significantly different among season. Therefore we must consider a counterplan to augment nutrition intake for them. In addition, it seems to be essential to micro nutrients to the food composition database to estimate dietary intakes more accurately.