• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.97-97
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• 2017

Plant genomes include heterochromatic loci that consist of repetitive sequences and transposable elements. LTR retrotransposon is the major class of transposons in advanced plants in terms of proportion in plant genome. The elements contribute not only to genome size but also to genome stability and gene expression. A number of cases have been reported transposon insertions near genic regions affect crop traits such as fruit pigments, stress tolerance, and yields. Functional LTR retrotransposons produce extrachromosomal DNA from genomic RNA by reverse transcription that takes place within virus-like-particles (VLPs). DECREASED DNA METHYLATION 1 (DDM1) plays important roles in maintaining DNA methylation of heterochromatin affecting all sequence contexts, CG, CHG, and CHH. Previous studies showed that ddm1 mutant exhibits massive transcription of retrotransposons in Arabidopsis, but only few of them were able to create new insertions into the genome. RNA-dependent RNA POLYMERASE 6 (RDR6) is known to function in restricting accumulation of transposon RNA by processing the transcripts into 21-22 nt epigenetically activated small interfering RNA (easiRNA). We purified VLPs and sequence cDNA to identify functional LTR retrotransposons in Arabidopsis ddm1 and ddm1rdr6 plants. Over 20 LTR copia and gypsy families were detected in ddm1 and ddm1rdr6 sequencing libraries and most of them were not reported for mobility. In ddm1rdr6, short fragments of ATHILA gypsy elements were detected. It suggests easiRNAs might regulate reverse transcription steps. The highest enriched element among transposon loci was previously characterized EVADE element. It has been reported that active EVADE element is more efficiently silenced through female germline than male germline. By genetic analyses, we found ddm1 and rdr6 mutation affect maternal silencing of active EVADE elements. DDM1-GFP protein accumulated in megaspore mother cell but was not found in mature egg cell. The fusion protein was also found in early embryo and maternal DDM1-GFP allele was more dominantly expressed in the embryo. We observed localization of DDM1-GFP in Arabidopsis and DDM1-YFP in maize and found the proteins accumulated in dividing zone of root tips. Currently we are looking at cell cycle dependency of DDM1 expression using maize system. Among 10 AGO proteins in Arabidopsis, AGO9 is specifically expressed in egg cell and shoot meristematic cells. In addition, mutation of AGO9 and RDR6 caused failure in maternal silencing, implying 21-22 nt easiRNA pathway is important for retrotransposon silencing in female gametophyte or/and early embryo. On the other hand, canonical 24 nt sRNA-directed DNA methylation (RdDM) pathways did not contribute to maternal silencing as confirmed by this study. Heat-activated LTR retrotransposon, ONSEN, was not silenced by DDM1 but the silencing mechanisms require RdDM pathways in somatic cells. We will propose distinct mechanisms of LTR retrotransposons in germline and somatic stages.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.10
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• pp.6135-6140
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• 2013

Background: Breast cancer is a common malignant tumor which affects health of women and multidrug resistance (MDR) is one of the main factors leading to failure of chemotherapy. This study was conducted to establish paclitaxel-resistant breast cancer cell line and nude mice models to explore underlying mechanisms of MDR. Methods: The breast cancer drug-sensitive cell line MCF-7 (MCF-7/S) was exposed in stepwise escalating paclitaxel (TAX) to induce a resistant cell line MCF-7/TAX. Cell sensitivity to drugs and growth curves were measured by MTT assay. Changes of cell morphology and ultrastructure were examined by optical and electron microscopy. The cell cycle distribution was determined by flow cytometry. Furthermore, expression of proteins related to breast cancer occurrence and MDR was tested by immunocytochemistry. In Vivo, nude mice were injected with MCF-7/S and MCF-7/TAX cells and weights and tumor sizes were observed after paclitaxel treatment. In addition, proteins involved breast cancer and MDR were detected by immunohistochemistry. Results: Compared to MCF-7/S, MCF-7/TAX cells had a higher resistance to paclitaxel, cross-resistance and prolonged doubling time. Moreover, MCF-7/TAX showed obvious alterations of ultrastructure. Estrogen receptor (ER) expression was low in drug resistant cells and tumors while expression of human epidermal growth factor receptor 2 (HER2) and Ki-67 was up-regulated. P-glycoprotein (P-gp), lung resistance-related protein (LRP) and glutathione-S-transferase-${\pi}$ (GST-${\pi}$) involved in the MDR phenotype of resistant cells and tumors were all overexpressed. Conclusion: The underlying MDR mechanism of breast cancer may involve increased expression of P-gp, LRP and GST-${\pi}$.

• The Transactions of the Korea Information Processing Society
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• v.6 no.8
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• pp.2233-2244
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• 1999

It is essential to guarantee high reliability of embedded real-time systems since the failure of such systems may result in large financial damage or threaten human life. Though many researches have devoted to fault tolerant mechanisms, most of them are object-level fault tolerant mechanisms that can detect errors occurred in a single object and treat the errors in object-level. As embedded real-time systems become more complex and larger, there exist faults that cannot be detected by or tolerated with object-level fault tolerance. Hence, system-level fault tolerance is needed. System-level fault tolerance examines the status of a system whether the system is normal or not by analyzing the status of objects. When an error is detected it should be capable of locating the fault and performing an appropriate recovery and reconfiguration action. In this paper, we propose RobustRTO(Robust Real-Time Object) that provides object-level fault tolerance capability and RMO(Region Monitor real-time Object) that offers system-level fault tolerance capability. Then we show how highly dependable fault tolerant systems can be modeled by RobustRTO and RMO. The model is presented based on real-time objects.

• Journal of Trauma and Injury
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• v.25 no.4
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• pp.247-253
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• 2012

Purpose: Hemorrhage is a main cause of death in trauma patients. The goal of this study is to describe the characteristics of trauma patients with massive bleeding and to evaluate the prognostic factors concerning their survival. Methods: This study was performed retrospectively and included trauma patients with massive bleeding who had been treated from March 2007 to August 2012. The inclusion criterion was patients who received more than 10 U of packed red blood cells within the first 24 hours after visiting the emergency department. Based on their medical records, we collected data in terms of demographic findings, mechanisms of injury, initial clinical and laboratory findings, methods for hemostasis (emergency surgery and/or angioembolization), transfusion, injury severity score (ISS), revised trauma score (RTS) and trauma and injury severity score (TRISS). We used the Mann-Whitney U test and Fisher's exact test to compare the variables between the patients that survived and those that did not. We performed a logistic regression analysis with the significant variables from the univariate test. Results: Thirty-two(32) patients were enrolled. The main mechanisms of injury were falls and motor vehicle accidents. The mean transfusion amount of packed red blood cells (PRBC) was 17.4 U. The mean elapsed time for the first hemostasis (surgery or embolization) was 3.5 hours. The initial technical success rates were 83.3%(15/18) in angioembolization and 66.7%(8/12) in surgery. The overall mortality rate was 34.4%(11/32). The causes of death were bleeding, brain swelling and multiple organ failure. The ISS(25.5 vs 46.3, p=0.000), TRISS(73.6 vs 45.1, p=0.034) and base excess(<-12 mmol/L, p=0.020) were significantly different between the patients who survived and those who did not. Conclusion: The ISS was a prognostic factor for trauma patients with massive bleeding.

• Journal of the Korean Geotechnical Society
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• v.36 no.11
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• pp.35-49
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• 2020

The lateral bearing capacity of the micropile depends on the installed conditions such as number, installation angle and spacing of the pile. Existing research on micropile has been limited to the evaluation of vertical bearing characteristics and suggestion of effective installation methods, and there are few studies on failure mechanisms such as failure mode. And most of the studies on the lateral bearing capacity of micropile are also on the 1-row micropile. Therefore, in this study, a model test was performed to evaluate the behavior and lateral bearing characteristics of a 2-row micropile when the installed conditions such as the installation length, angle, and spacing of the pile were different. As a result of the model test, when the installation angle is θ > 0° (Not cross installation), the lateral bearing capacity of 2-row micropile depends on the spacing of the piles, and the installation angle θ = +30° was the most effective for increasing the bearing capacity. In addition, when the installation angle is θ < 0° (Overlap installation), it depends on the spacing and angle of the pile, and the condition of installation angle θ = -15° was found to be the most effective for increasing the bearing capacity.

• Journal of the Korean Society for Nondestructive Testing
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• v.29 no.5
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• pp.485-492
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• 2009

The thermal fatigue crack(TFC) is one of the life-limiting mechanisms at the nuclear power plant operating conditions. In order to evaluate the structural integrity, various non-destructive test methods such as radiographic test, ultrasonic test and eddy current are used in the industrial field. However, these methods have restrictions that defect detection is possible after the crack growth. For this reason, acoustic emission testing(AET) is becoming one of powerful inspection methods, because AET has an advantage that possible to monitor the structure continuously. Generally, every mechanism that affects the integrity of the structure or equipment is a source of acoustic emission signal. Therefore the noise filtering is one of the major works to the almost AET researchers. In this study, acoustic emission signal was collected from the pipes which were in the successive thermal fatigue cycles. The data were filtered based on the results from previous experiments. Through the data analysis, the signal characteristics to distinguish the effective signal from the noises for the TFC were proven as the waveform difference. The experiment results provide preliminary information for the acoustic emission technique to the continuous monitoring of the structure failure detection.

• Journal of Energy Engineering
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• v.27 no.2
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• pp.1-13
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• 2018

The proportion of natural gas-fueled power generation is expanding due to the change of domestic energy policy pursuing reduction of dust and increasing clean energy consumption. Natural gas fuels used for the combined-cycle power plants and the district-heating power plants are operated at high temperature and high pressure in the fuel supply system. Accidents due to leakage of the gas such as fire and explosion should be prevented by applying risk management techniques. In this study, risk assessment was performed on the natural gas fuel supply system of a combined power plant based on the API RP 581 RBI code. For the application of the API RBI code, lines and segments of the evaluation target system were identified. Operational data and input information were analyzed for the calculations of probability of failure and consequence of failure. The results of the risk assessment were analyzed over time from the initial installation time. In the code-based evaluation, the gas fuel supply system was mainly affected by thinning, external damage, and mechanical fatigue damage mechanisms. As the operating time passes, the risk is expected to increase due to the external damage caused by the CUI(Corrosion Under Insulation).

• Restorative Dentistry and Endodontics
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• v.33 no.6
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• pp.560-569
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• 2008

Microorganism survived in the root canal after root canal cleaning and shaping procedure is a main cause of root canal treatment failure. There are several mechanisms for the bacteria to survive in the root canal after chemomechanical preparation and root canal irrigation. Bacteria organized as biofilm has been suggested as an etiology of persistent periapical lesion. Recent studies were focus on removal of Enterococcus faecalis biofilm due to the report that the persistence of this bacteria after root canal treatment may be associated with its ability to form biofilm. Several investigations demonstrated that current root canal treatment protocol including use of NaOCl, EDTA and Chlorhexidine as irrigants is quite effective in eliminating E. faecalis biofilm. However, this microorganism still can survive in inaccessible areas of root canal system and evade host immune response, suppress immune activity and produce biofilm. Up to date, there is no possible clinical method to completely get rid of bacteria from the root canal. Once the root canal treatment failure occurred, and conventional treatment incorporating current therapeutic protocol has failed, periapical surgery or extraction should be considered rather than prolong the in effected retreatment procedure.

• Journal of the Institute of Electronics and Information Engineers
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• v.51 no.10
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• pp.34-40
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• 2014

With the economical data service delivery, the packet transport network (PTN) technologies have emerged as an important player in the next-generation transport networks. As the PTN continues to make such considerable progress, it is being challenged by network providers who need rapid and reliable recovery capabilities to guarantee the availability of their services. This paper introduces several fault detection mechanisms for a client signal failure in packet transport networks and proposes a reliable transmission method of IP flows from routers using the combination of Ethernet services. Based on the first fault detection methods, client signal fault is detected within tens or hundreds of milliseconds. It enables the client network devices to perform their own recovery processes within one second. The second mechanism enables failed Ethernet services to be bypassed via other Ethernet services over disjoint paths, so as to contribute on reducing packet loss of IP traffic.

• Composites Research
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• v.29 no.2
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• pp.45-52
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• 2016

An understanding of the failure mechanisms of the adhesive layer is decisive in interpreting the performance of a particular adhesive joint because the delamination is one of the most common failure modes of the laminated composites such as the fiber metal laminates. The interface between different materials, which is the case between the metal and the composite layers in this study, can be loaded through a combination of fracture modes. All loads can be decomposed into peel stresses, perpendicular to the interface, and two in-plane shear stresses, leading to three basic fracture mode I, II and III. To determine the load causing the delamination growth, the energy release rate should be identified in corresponding criterion involving the critical energy release rate ($G_C$) of the material. The critical energy release rate based on these three modes will be $G_{IC}$, $G_{IIC}$ and $G_{IIIC}$. In this study, to evaluate the fracture behaviors in the fracture mode I and II of the adhesive layer in fiber metal laminates, the double cantilever beam and the end-notched flexure tests were performed using the reference adhesive joints. Furthermore, it is confirmed that the experimental results of the adhesive fracture toughness can be applied by the comparison with the finite element analysis using cohesive zone model.