• Asian Pacific Journal of Cancer Prevention
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• 제13권5호
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• pp.2409-2414
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• 2012

Objective: Traditional Chinese herbal medicines have a very long history. Rosa roxburghii Tratt and Fagopyrum cymosum are two examples of plants which are reputed to have benefits in improving immune responses, enhancing digestive ability and demonstrating anti-aging effects. Some evidence indicates that herbal medicine soups containing extracts from the two in combination have efficacy in treating malignant tumors. However, the underlying mechanisms are far from well understood. The present study was therefore undertaken to evaluate anticancer effects and explore molecular mechanisms in vitro. Methods: Proliferation and apoptosis were assessed with three carcinoma cell lines (human esophageal squamous carcinoma CaEs-17, human gastric carcinoma SGC-7901 and pulmonary carcinoma A549) by MTT assay and flow cytometry, respectively, after exposure to extract from Rosa roxburghii Tratt (CL) and extract from Fagopyrum cymosum (FR). $IC_{30}$ of CL and FR were obtained by MTT assay. Tumor cells were divided into four groups : control with no exposure to CL or FR; CL with $IC_{30}$ CL; FR with $IC_{30}$ FR; CL+FR group with 1/2 ($IC_{30}$ CL + $IC_{30}$ FR). RT-PCR and Western blot analysis were used to detect the expression of Ki-67, Bax and Bcl-2 at mRNA and protein levels. Results: Compared with the CL or FR groups, the combination of CL+FR showed significant inhibition of cell growth and increase in apoptosis; the mRNA and protein expression levels of Ki-67 and Bcl-2 in CL+FR group were all greatly decreased, while the expression of Bax was markedly increased. Conclusions: These results indicate that the synergistic antitumor effects of combination of CL and FR are related to inhibition of proliferation and induction of apoptosis.

• 한국자원식물학회지
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• 제11권3호
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• pp.335-344
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• 1998

Percicarp anatomy of Fagophrum was examined on the basis of 12 species and two subspecies to contribute to a better understanding of specific phylogenetic relationship within genus. Examined species have a similar mature pericarp structure, but differences among the species are found with respect to whether or not sclerotic cells are present, and what kind of is the sclerotic cell shape in theexocarp. By the comparisons with pericarp anatomical structure, they are classified into three groups. First clade is composed of F. esculentum , F. esculentum ssp. ancestralis and F. homotropicum ; second clade is consisted of F. tataricum, F. tataricum ssp. potanini and Fl cymosum ; third clade is composed of f. callianthum,F. capillatum, F. gracilipes, F. leptopodum, F.lineare, F. pleioramosum, F.statice and F.urophyllum. The phylogey based on pericarp characters was considerably consistent with ones proposed by previous authors. It also suggested that pericarp characters are useful informatino for deduceing phylogenetic phylogenetic realtionship within geneus Fagopyrum. On the other hand, morphological character evolution indicated that there are tow synapomorphies in genus. There,it was suggested that these species having selfing and homostylous characters are evolved form heterostylous and ourcrossing species.

• 한국자원식물학회지
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• 제18권2호
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• pp.246-250
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• 2005

아시아 지역에서 수집한 야생 쓴메밀의 종자 및 식물체 내의 루틴함량을 조사하였다. 쓴메밀은 종자 및 식물 전체부위에서 숙근메밀이나 단메밀보다 높은 루틴 함량을 나타냈다. 특히, 꽃은 숙근메밀의 2.2배, 단메밀의 9.5배, 줄기는 각각 3.1배와 24.9배, 종자는 3.2와 65배나 높았다. 3종에 대한 식물체 부위별 루틴함량은 모두 꽃>잎>종자>줄기>뿌리의 순으로 높았다. 종피색에 따른 루틴함량은 암회색>검은색>갈색, 회갈색 순의 경향을 나타냈으며, 종자의 형태는 Slender>Notched>Round 순으로 루틴함량이 높았다. 재배종은 야생종에 비해 루틴함량이 잎은 1.3배, 줄기가 1.4배, 종자가 1.2배의 높은 경향을 보였다. 부탄지역 수집품종은 잎, 줄기, 종자의 루틴함량이 다른 지역보다 높은 경향을 보였고, 슬로베니아 및 파키스탄지역 수집품종은 잎, 줄기에서, 인도 및 네팔지역 수집품종은 종자에서 가장 낮은 루틴함량의 경향을 보였다.