• Title/Summary/Keyword: Factorial experiment

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The Effects of Different Copper (Inorganic and Organic) and Energy (Tallow and Glycerol) Sources on Growth Performance, Nutrient Digestibility, and Fecal Excretion Profiles in Growing Pigs

  • Huang, Y.;Yoo, J.S.;Kim, H.J.;Wang, Y.;Chen, Y.J.;Cho, J.H.;Kim, I.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.5
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    • pp.573-579
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    • 2010
  • This study was conducted to determine the effects of different copper (inorganic and organic) and energy (tallow and glycerol) sources on growth performance, nutrient digestibility, gas emission, diarrhea incidence, and fecal copper concentration in growing pigs by using a 2${\times}$2 factorial design. In this trial, 96 pigs (63 d of age) were employed, with an average initial weight of 28.36${\pm}$1.14 kg. The dietary treatments were i) basal diet with 134 ppm copper (Korea recommendation) as $CuSO_4$+tallow; ii) basal diet with 134 ppm Cu as $CuSO_4$+glycerol; iii) basal diet with 134 ppm copper as CuMet+tallow; and iv) basal diet with 134 ppm copper as CuMet+ glycerol. Throughout the entire experimental period, no differences were noted among treatment groups with regard to the magnitude of improvement in ADG (average daily gain), ADFI (average daily feed intake) and G/F (gain:feed) ratios. The nitrogen (N) digestibility of pigs fed on diets containing organic copper was improved as compared with that observed in pigs fed on diets containing inorganic copper (p<0.05). An interaction of copper${\times}$energy was observed in the context of both nitrogen (p<0.05) and energy (p<0.01) digestibility. Ammonia emissions were significantly lower in the organic copper-added treatment groups than in the inorganic copperadded treatment groups (p<0.05). Mercaptan and hydrogen sulfide emissions were reduced via the addition of glycerol (p<0.05). No significant effects of copper or energy source, or their interaction, were observed in reference to diarrhea appearance and incidence throughout the entirety of the experimental period. The copper concentration in the feces was significantly lower in the organic copper source treatment group than was observed in the inorganic copper source treatment group (p<0.05). The results of this experiment show that organic copper substituted for inorganic copper in the diet results in a decreased fecal copper excretion, but exerts no effect on performance. The different energy (tallow and glycerol) sources interact with different copper sources and thus influence nutrient digestibility. Glycerol supplementation may reduce the concentrations of odorous sulfuric compounds with different Cu sources.

Effect of energy density and virginiamycin supplementation in diets on growth performance and digestive function of finishing steers

  • Navarrete, Juan D.;Montano, Martin F.;Raymundo, Constantino;Salinas-Chavira, Jaime;Torrentera, Noemi;Zinn, Richard A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.10
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    • pp.1396-1404
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    • 2017
  • Objective: This study was determined the influence of virginiamycin supplementation on growth-performance and characteristics of digestion of cattle with decreasing dietary net energy value of the diet for maintenance ($NE_m$) from 2.22 to 2.10 Mcal/kg. Methods: Eighty crossbred beef steers ($298.2{\pm}6.3kg$) were used in a 152-d performance evaluation consisting of a 28-d adaptation period followed by a 124-d growing-finishing period. During the 124-d period steers were fed either a lesser energy dense (LED, $2.10Mcal/kg\;NE_m$) or higher energy dense (HED, $2.22Mcal/kg\;NE_m$) diet. Diets were fed with or without 28 mg/kg (dry matter [DM] basis) virginiamycin in a $2{\times}2$ factorial arrangement. Four Holstein steers ($170.4{\pm}5.6kg$) with cannulas in the rumen (3.8 cm internal diameter) and proximal duodenum were used in $4{\times}4$ Latin square experiment to study treatment effects on characteristics of digestion. Results: Neither diet energy density nor virginiamycin affected average daily gain (p>0.10). As expected, dry matter intake and gain efficiency were greater (p<0.01) for LED- than for HED-fed steers. Virginiamycin did not affect estimated net energy value of the LED diet. Virginiamycin increased estimated NE of the HED diet. During daylight hours when the temperature humidity index averaged $81.3{\pm}2.7$, virginiamycin decreased (p<0.05) ruminal temperature. Virginiamycin did not influence (p>0.10) ruminal or total tract digestion. Ruminal (p = 0.02) and total tract digestion (p<0.01) of organic matter, and digestible energy (p<0.01) were greater for HED vs LED. Ruminal microbial efficiency was lower (p<0.01) for HED vs LED diets. Conclusion: The positive effect of virginiamycin on growth performance of cattle is due to increased efficiency of energy utilization, as effects of virginiamycin on characteristics of digestion were not appreciable. Under conditions of high ambient temperature virginiamycin may reduce body temperature.

Effects of processing, particle size and moisturizing of sorghum-based feeds on pellet quality and broiler production

  • da Silva, Patricia Garcia;Oliveira, Luana Martins Schaly;de Oliveira, Nayanne Rodrigues;de Moura, Fabio Ataides Junior;Silva, Maura Regina Sousa;Cordeiro, Deibity Alves;Minafra, Cibele Silva;dos Santo, Fabiana Ramos
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.1
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    • pp.98-105
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    • 2018
  • Objective: This study aimed to assess the effect of pelleted and expanded sorghum-based feeds prepared with different moisture levels and particle size of ingredients on metabolizable energy, ileal digestibility of amino acids and broiler performance. Methods: The experiment was performed with 720 male broiler chicks of the Cobb strain, with treatments of six replications, with 15 birds each; they were arranged in a completely randomized design and $2{\times}2{\times}2$ factorial scheme (pelleted or expanded feed processing, 0.8% or 1.6% moisture addition in the mixer, and particle size of 650 or 850 microns). Results: Higher pellet quality (pellets, % and pellet durability index [PDI]) was obtained in expanded diets and inclusion of 1.6% moisture. The particle size of 850 microns increased the PDI of final diet. All studied treatments had no significant effect on weight gain and broiler carcass and cut yields. Lower feed conversion occurred for birds fed pelleted feed at 42 d. The highest apparent metabolizable energy (AME) and apparent metabolizable energy corrected to zero nitrogen balance (AMEn) values of feed in the initial rearing phase (10 to 13 days) were observed in birds fed pelleted feed or for feed prepared with 1.6% moisture. The highest ileal digestibility coefficients of amino acids were obtained with the consumption of pelleted feed prepared with a particle size of 650 microns and 1.6% moisture. Conclusion: Pelleted feed prepared with a milling particle size of 650 microns and 1.6% moisture provided increased ileal digestibility of amino acids and AMEn in the starter period. However, the expanded feed improved pellet quality and feed conversion of broilers at 42 days of age. We conclude that factors such as moisture, particle size and processing affect the pellet quality, and therefore should be considered when attempting to optimize broiler performance.

Growth of the Juvenile 01ive Flounder (Paralichthys olivaceus) Fed the Diets at Different Feeding Frequencies (사료 공급 횟수가 넙치 치어의 성장에 미치는 영향)

  • LEE Sang-Min;SEO Chong-Hyun;CHO Young-Sik
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.1
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    • pp.18-21
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    • 1999
  • A feeding experiment was conducted to determine the effects of feeding frequency on growth and body composition in juvenile olive flounder (Paralichthys olivaceus). Triplicate groups of the 40 fish averaging 1.6 g were fed the 2 different commercial diets (D-1 and D-2) and moist pellet (MP) contained frozen horse mackerel and commercial binder meal at different feeding frequencies of 4 times daily, 3 times daily, 2 times daily, once a day or once in 2 days. Growth and feed intake of fish were affected by diets and feeding frequencies (P<0.05). Feed intake ($\%$ of body wt.) in the all diet groups was significantly decreased according to feeding frequency decreased (P<0.05). Weight gain of fish fed the each diet were increased according to feeding frequency increased, although no significant differences were observed between 3 times and 4 times daily (P>0,05). If the same feeding frequency, feed intake and growth of fish fed the moist pellet were lower (P<0.05) than those of commercial diets (D-1 or D-2), probably due to the relatively high moisture content or unbalanced nutrients. Whole body lipid content of fish fed the each diet at once in 2 days was the lowest among feeding frequencies. These results indicate that 3 times daily feeding regimen may be more effective than that of 4 times daily and moist pellet in this study will not be used as a practical type of diet for juvenile olive flounder weighing between 1,6 g and 4 g.

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Effects of varying CO2, Nutrient and Light Irradiance Levels on the Growth of Ulva australis at Germling, Juvenile, and Adult Stages (해수의 CO2와 영양염 농도 및 조도가 구멍갈파래(Ulva australis) 배아, 유엽과 성체의 생장에 미치는 영향)

  • Jeon, Da Vine;Na, Yeon Ju;Yu, Ok Hwan;Choi, Han Gil
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.48 no.1
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    • pp.96-103
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    • 2015
  • The effects of $CO_2$ concentration, nutrient levels, and irradiance on the growth of germlings and juveniles, and on the photosynthesis of adults were examined in a green tide alga, Ulva australis. We used a factorial experimental design with two $CO_2$ concentrations (380 and 750 ppm), two nutrient levels (control and PES medium), and two irradiance levels (50 and $100{\mu}mol$ photons $m^{-2}s^{-1}$). Germlings grew best ($664.15{\pm}61.45{\mu}m$ in length) under conditions of 750 ppm, PES, and $100{\mu}mol$ photons $m^{-2}s^{-1}$ after 10 days in culture. Relative growth rates (RGR) of the juveniles were greatest (4.41% $day^{-1}$) under conditions of 750 ppm, PES, and $50{\mu}mol$ photons $m^{-2}s^{-1}$ after 5 days in culture. Photosynthetic efficiency ($F_v/F_m$) of the adult discs was $0.73{\pm}0.05$ before the experiment and reached a maximum ($0.83{\pm}0.01$) under conditions of 750 ppm, control, and $50{\mu}mol$ photons $m^{-2}s^{-1}$ after 5 days in culture. Growth (germlings and juveniles) and photosynthesis (adult discs) of Ulva australis increased when $CO_2$ levels were 750 ppm. Additionally, the optimal irradiance for growth and photosynthesis differed among stages, wherein germlings grew best at $100{\mu}mol$ photons $m^{-2}s^{-1}$, juveniles grew best at $50{\mu}mol$ photons $m^{-2}s^{-1}$, and adults photosynthesized most at $50{\mu}mol$ photons $m^{-2}s^{-1}$. The performance of Ulva australis at all examined life stages was enhanced under the PES nutrient treatment. In conclusion, the physiological responses of U. australis to varying $CO_2$, nutrient, and irradiance levels differed slightly among life stages. However, growth and photosynthesis always increased with elevated $CO_2$ and nutrient concentrations. These results indicate that U. australis green tide blooms might occur more frequently in coastal areas if $CO_2$ and nutrient concentrations increase.

Yield and Chemical Composition of Cassava Foliage and Tuber Yield as Influenced by Harvesting Height and Cutting Interval

  • Khang, Duong Nguyen;Wiktorsson, Hans;Preston, Thomas R.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.7
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    • pp.1029-1035
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    • 2005
  • A 3${\times}$4 factorial field experiment with a complete randomised split-plot design with four replicates was conducted from June 2002 to March 2003 at the experimental farm of the Nong Lam University, Ho Chi Minh City, Vietnam, to determine effects of different harvesting heights (10, 30 and 50 cm above the ground) and cutting intervals (45, 60, 90 and 285 days) on yield of foliage and tubers, and chemical composition of the foliage. Cassava of the variety KM 94 grown in plots of 5 m${\times}$10 m at a planting distance of 30 cm${\times}$50 cm was hand-harvested according to respective treatments, starting 105 days after planting. Foliage from the control treatment (285 days) and all tubers were only harvested at the final harvest 285 days after planting. Dry matter and crude protein foliage yields increased in all treatments compared to the control. Mean foliage dry matter (DM) and crude protein (CP) yields were 4.57, 3.53, 2.49, and 0.64 tonnes DM $ha^{-1}$ and 939, 684, 495 and 123 kg CP $ha^{-1}$ with 45, 60, 90 and 285 day cutting intervals, respectively. At harvesting heights of 10, 30 and 50 cm the DM yields were 4.27, 3.67 and 2.65 tonnes $ha^{-1}$ and the CP yields were 810, 745 and 564 kg $ha^{-1}$, respectively. The leaf DM proportion was high, ranging from 47 to 65%. The proportion of leaf and petiole increased and the stem decreased with increasing harvesting heights and decreasing cutting intervals. Crude protein content in cassava foliage ranged from 17.7 to 22.6% and was affected by harvesting height and cutting interval. The ADF and NDF contents of foliage varied between 22.6 and 30.2%, and 34.2 and 41.2% of DM, respectively. The fresh tuber yield in the control treatment was 34.5 tonnes $ha^{-1}$. Cutting interval and harvesting height had significant negative effects on tuber yield. The most extreme effect was for the frequent foliage harvesting at 10 cm harvesting height, which reduced the tuber yield by 72%, while the 90 day cutting intervals and 50 cm harvesting height only reduced the yield by 7%. The mean fresh tuber yield decreased by 56, 45 and 27% in total when the foliage was harvested at 45, 60 and 90 day cutting intervals, respectively. It is concluded that the clear effects on quantity and quality of foliage and the effect on tuber yield allow alternative foliage harvesting principles depending on the need of fodder for animals, value of tubers and harvesting cost. An initial foliage harvest 105 days after planting and later harvests with 90 days intervals at 50 cm harvesting height increased the foliage DM and CP yield threefold, but showed only marginal negative effect on tuber yield.

A Study on Flow Distribution to Flocculation Basins Using DOE and RSA (실험계획법과 반응표면분석법을 적용한 응집지로의 유량분배에 관한 연구)

  • Kim, Seong-Jae;Kyung, Gyu-Sun;Jeong, Heui-Jung;Kim, Hyeong-Seop;Yang, Sa-Sun
    • Journal of Korean Society of Environmental Engineers
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    • v.35 no.12
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    • pp.918-928
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    • 2013
  • The inequitable flow distribution to flocculation basins is an important problem faced in many water treatment plants. This is caused by the structure of a distribution channel, the height differences of outlet weirs etc. But, a modified approach for the structures has no effectiveness to achieve flow equality. The aim of this study is to reduce the inequality by adopting optimized inlet valve opening (%) of the flocculation basins using DOE (Design of Experiments) and RSA (Response Surface Analysis). The inlet valve openings (%) and inflow distributions (%) of 6 paralleled basins were set as factors (X) and characteristics(Y) respectively. 2 level factorial experiments and RSA were conducted for optimization and regression analysis (Y = f(X) + Const.). Adopting the optimized inlet valve opening (%) at field, standard deviation of flow distribution (%) and effluent turbidity was declined from 3.80% to 0.42% and from 0.29 NTU to 0.03 NTU respectively.

Analysis of Broken Rice Separation Efficiency of a Laboratory Indented Cylinder Separator

  • Kim, Myoung Ho;Park, Seung Je
    • Journal of Biosystems Engineering
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    • v.38 no.2
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    • pp.95-102
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    • 2013
  • Purpose: Using a laboratory indented cylinder separator, broken rice separation experiments were conducted and the characteristics of the separation process were studied to provide information for developing a prototype indented cylinder broken rice separator. Methods: Rice (Ilmi variety) milled in a local RPC was used for the experiment. Rice kernels were classified into four groups according to their length l; whole kernels (I > 3.75 mm), semi-whole kernels (2.5 < I < 3.75 mm), broken kernels (1.75 < I < 2.5 mm), and foreign matters (I < 1.75 mm). A laboratory grain cleaner, Labofix '90 (Schmidt AG, Germany) was used for the experiments. Experiments were designed as a $4{\times}4$ factorial arrangement in randomized blocks with three replications. Cylinder rotational speeds (17, 34, 51, 68 rpm) and trough angles (15, 37.5, 60, $82.5^{\circ}$) were the two factors and feed rates (25, 50 kg/h), indent shapes (Us, $S_1$ type), and indent sizes (2.5, 3.75 mm) were treated as the blocks. Two 125 g samples and one 125 g sample were taken at the cylinder outlet and from the trough, respectively. The whole, semi-whole, and broken kernel weight ratio of the samples and feed was determined by a rice sizing device. From these weight ratios, purities, degrees of extraction and coefficient of separation efficiency were calculated. Results: Trough angle, cylinder speed, and their interaction on the coefficient of separation efficiency were statistically significant. Cylinder speed of 17, 34, and 51 rpm made the most effective separation when the trough angle was $15^{\circ}$ or $37.5^{\circ}$, $60^{\circ}$, and $82.5^{\circ}$, respectively. Maximum values of coefficient of separation efficiency were in the range of 60 to 70% except when the indent size was 2.5 mm and were recorded for the combinations of low cylinder speed (17 rpm) with medium trough angle ($37.5^{\circ}$ or $60^{\circ}$). Indent shape did not appear to make any noticeable difference in separation efficiency. Conclusions: Due to the interaction effect, the trough angle needs to be increased appropriately when an increase in cylinder speed is made if a rapid drop of effectiveness of separation should be avoided. In commercial applications, $S_1$ type indents are preferred because of their better manufacturability and easier maintenance. For successful separation of broken kernels, the indent size should be set slightly bigger than the actual sizes of broken kernels: an indent size of 3.0 mm for separating broken kernels shorter than 2.5 mm.

Heading Date of Wheat and Barley III. Factorial Analysis of Wheat Varieties on Earliness (맥류의 출수기에 관한 연구 III. 소맥품종의 조숙성 변이에 관한 요인분석)

  • 하용웅;맹돈재
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.29 no.3
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    • pp.254-260
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    • 1984
  • As a basic study for development of early wheats an experiment was carried out at four sites of Korea by use of 60 varieties. Analysis of variance for heading and maturing dates revealed that there were highly significant among varieties, but not significant among variety x location-interactions, thus indicating the stable response for earliness at all locations of each variety. Grain filling periods of each variety showed the stable response at all locations like those of heading and maturing dates, however, there showed significance among locations for early group, and among varieties for late group. High correlations were between growth habit and winter survival for early varieties, and between shortday length and grain filling period for late one. Interior factors most closely related to heading date were growth habit and winter survival, and winter survival showed highly negative correlation with heading date. It needs to breed the early wheats adaptable for southern and/or northern areas separatively. The earlier heading and maturing dates of wheats were, the shorter internodes of culm are, appearing the outstanding phenomenon in early wheats. Heading date appeared negative correlations with test weight and with number of culm before winter, but showed positive correlation with number of kernels/spike.

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Effects of Kaolin (Natural Ligneous Clay) Supplementation on Performance and Egg Quality in Laying Hens (고령토(천연목질점토)의 급여가 산란계의 산란성적과 계란품질에 미치는 영향)

  • Kim, Jin-Soo;Jo, Jong-Kwan;Yoon, Se-Young;Yun, Ku;Kwon, Il-Kyung;Chae, Byung-Jo
    • Journal of Animal Science and Technology
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    • v.53 no.2
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    • pp.133-138
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    • 2011
  • Two experiments were conducted to determine the effects of supplementing different levels of kaolin (Exp. 1) and effects of age of layers and levels of kaolin (Exp. 2) on the performance, egg and shell quality parameters of laying hens. In Exp.1, 64 laying hens of 58 wks age were randomly allotted to 4 treatments of 16 hens in each. Dietary treatments were basal diet supplemented with 0.0, 0.2, 0.4 and 0.6% kaolin. In Exp. 2 96 laying hens were divided into 6 groups of 16 hens each in a 2 ${\times}$ 3 factorial arrangement (two different ages, 30 and 58 wk and three levels of kaolin, 0.0, 0.2 and 0.4%). In both the experiment there were linear decreases in feed intake (P<0.001), improvement in FCR (P<0.05), and egg production (P<0.05), with increasing dietary kolin level. Supplementation of kaolin had no effect on the egg and shell quality parameters in both experiments. Thus, it is concluded that kaolin may improve performance, and 0.2% level showed the best laying performance.