• 한국콘텐츠학회논문지
• /
• 제19권3호
• /
• pp.500-509
• /
• 2019

본 연구는 체질푸드가 간호사의 자연치유력증진에 있어서 스트레스 감소와 NK세포 활성도에 미치는 영향에 대해 밝히고자 하였으며, 이를 통해 체질푸드가 인간의 건강 예방과 치유에 효과적인지를 고찰하였다. 연구는 경기도 한 종합병원에서 현재 1년 이상 근무 경력이 있는 간호사 22명을 대상으로 30일 동안 진행되었다. 본 연구에서는 스트레스 측정을 위해 유비오맥파측정기(uBioClip v70)를 사용하였고 NK세포 활성도에 대해서는 혈액검사를 통해 진행하였다. 수집된 자료처리는 통계분석 프로그램인 SPSS 21을 통해 대응표본 t검증으로 진행하였으며, 황제내경과 장상학에 근거한 체질분류 후 참여자의 체질에 알맞은 체질 푸드를 제공하여 실시하였다. 연구 결과, 체질푸드의 스트레스 감소에 미치는 영향은 유의한 정(+)의 효과를 나타내었고, NK세포 활성도에 대해서 체질푸드가 효과적인 것으로 나타났다. 본 연구는 의의는 다음과 같다. 첫째, 현대인의 건강을 위협하는 주 요소인 스트레스에 대해 체질푸드를 통한 자연치유력 강화가 스트레스에 대한 저항력을 높여줌으로써 질병에 대한 예방 효과가 있다는 점이다. 둘째, 질병의 예방과 치료에 있어서 면역세포 활성도는 매우 중요한데, 체질푸드를 통한 NK세포 활성도를 높여줌으로써 인체의 자연치유력을 증대시킬 수 있다는 점이다.

• Yonsei Medical Journal
• /
• 제59권9호
• /
• pp.1096-1106
• /
• 2018

Purpose: Alzheimer's disease (AD) is the sixth most common cause of death in the United States. MicroRNAs have been identified as vital players in neurodegenerative diseases, including AD. microRNA-128 (miR-128) has been shown to be dysregulated in AD. This study aimed to explore the roles and molecular mechanisms of miR-128 in AD progression. Materials and Methods: Expression patterns of miR-128 and peroxisome proliferator-activated receptor gamma ($PPAR-{\gamma}$) messenger RNA in clinical samples and cells were measured using RT-qPCR assay. $PPAR-{\gamma}$ protein levels were determined by Western blot assay. Cell viability was determined by MTT assay. Cell apoptotic rate was detected by flow cytometry via double-staining of Annexin V-FITC/PI. Caspase 3 and $NF-{\kappa}B$ activity was determined by a Caspase 3 Activity Assay Kit or $NF-{\kappa}B$ p65 Transcription Factor Assay Kit, respectively. Bioinformatics prediction and luciferase reporter assay were used to investigate interactions between miR-128 and $PPAR-{\gamma}$ 3'UTR. Results: MiR-128 expression was upregulated and $PPAR-{\gamma}$ expression was downregulated in plasma from AD patients and $amyloid-{\beta}$ $(A{\beta})-treated$ primary mouse cortical neurons (MCN) and Neuro2a (N2a) cells. Inhibition of miR-128 decreased $A{\beta}-mediated$ cytotoxicity through inactivation of $NF-{\kappa}B$ in MCN and N2a cells. Moreover, $PPAR-{\gamma}$ was a target of miR-128. $PPAR-{\gamma}$ upregulation attenuated $A{\beta}-mediated$ cytotoxicity by inactivating $NF-{\kappa}B$ in MCN and N2a cells. Furthermore, $PPAR-{\gamma}$ downregulation was able to abolish the effect of anti-miR-128 on cytotoxicity and $NF-{\kappa}B$ activity in MCN and N2a cells. Conclusion: MiR-128 inhibitor decreased $A{\beta}-mediated$ cytotoxicity by upregulating $PPAR-{\gamma}$ via inactivation of $NF-{\kappa}B$ in MCN and N2a cells, providing a new potential target in AD treatment.

• 자원ㆍ환경경제연구
• /
• 제30권2호
• /
• pp.237-268
• /
• 2021

에너지전환 정책의 가속화로 변동성 재생에너지가 가파르게 증가하면서 계통수용비용이 빠르게 상승하고 있다. 변동성 재생에너지 증가는 기존 전통적 발전자원의 이용률을 하락시켜서 전력공급에 비효율성을 가중시키는데 이에 대한 해결책으로 수요자원이 주목받고 있다. 본 연구에서는 수요자원 중 큰 잠재력을 가지고 있는 전기차 수요가 재생발전에 대한 유연성 자원으로 활용될 경우 전력공급비용을 얼마나 경감시킬 수 있는지 9차 전력수급계획을 반영하여 분석하였다. 분석모형으로 재생발전의 확률적 특성을 사실적으로 반영할 수 있는 확률적 전력시스템 최적화 모형을 적용해서 재생에너지가 유발하는 비용과 전기차 수요자원의 편익을 분석하였다. 분석결과 계시별 요금제보다 가상발전소 기반의 직접제어방식이 편익이 더 높고, 발전구성에서 재생에너지의 비중이 높아질수록 편익이 더 높아지는 것으로 나타났다. 전기차 수요자원의 구현비용인 중개사업자 수수료와 배터리마모비용을 고려한 순편익 추정결과, 충방전이 가능한 가상발전소 방식의 경우 월평균 운행비용의 67~85% 수준으로 나타났다. 이러한 수요자원 순편익이 소비자에게 효과적으로 분배되는 요금체계가 적용될 경우 시장참여유인이 높을 것으로 추정된다.

• 정보관리학회지
• /
• 제38권4호
• /
• pp.113-128
• /
• 2021

본 연구는 코로나바이러스감염증-19(이하 코로나바이러스) 백신에 대한 사회적 의견을 파악하기 위해 트위터에서 작성된 백신 관련 게시물들을 분석하였다. 2020년 3월 16일부터 2021 3월 15일까지 1년간 트위터에서 작성된 코로나바이러스 백신 이름을 키워드로 포함한 45,413개의 게시물을 수집하여 분석하였다. 데이터 수집을 위해 활용된 코로나바이러스 백신 키워드는 총 12개이며, 수집된 게시물 수순으로 '화이자', '아스트라제네카', '모더나', '얀센', '노바백스', '시노팜', '시노백', '스푸트니크', '바라트', '캔시노', '추마코프', '벡토르'이다. 수집된 게시물들은 수기와 자동화된 방법을 동시 활용하여 키워드 분석, 감성 분석, 및 토픽모델링을 통하여 백신들에 대한 의견을 탐색하였다. 연구결과에 따르면 전반적으로 백신에 대한 부정적인 반응이 많았으며, 백신 접종 후유증에 대한 불안 및 백신의 효능에 대한 불신이 백신들에 대한 부정적인 주요 요소로 파악되었다. 이와는 반대로, 백신 접종에 따른 코로나바이러스 확산 억제에 대한 기대감이 백신에 대한 긍정적인 사회적 요소인 것을 확인할 수 있었다. 본 연구는 기존의 선행연구들이 뉴스 등 대중매체 데이터를 통해 코로나바이러스 백신에 대한 사회적 분위기를 파악하고자 했던 것과 달리, 소셜 미디어 데이터 수집 및 이를 활용한 키워드 분석, 감성 분석, 토픽 모델링 등의 여러 분석방법들을 사용하여 대중들의 의견을 파악하는 것으로 학술적 의의를 지닌다. 또한, 본 연구의 결과는 백신에 대한 사회적 분위기를 반영한 백신 접종 권장 정책 수립 기여라는 실질적 함의를 시사한다.

• Nuclear Engineering and Technology
• /
• 제54권2호
• /
• pp.479-485
• /
• 2022

At the Korea Institute of Radiological and Medical Sciences, physical human phantoms were developed to evaluate various radiation protection quantities, based on the mesh-type reference computational phantoms of the International Commission on Radiological Protection. The physical human phantoms were fabricated such that a radiophotoluminescent glass dosimeter (RPLGD) with a Tin filter, namely GD-352M, could be inserted into them. A Tin filter is used to eliminate the overestimated signals in low-energy photons below 100 keV. The measurement uncertainty of the RPLGD reader system based on GD-352M should be analyzed for obtaining reliable protection quantities before using it for practical applications. Generally, the measurement uncertainty of RPLGD systems without Tin filters is analyzed for quality assurance of radiotherapy units using a high-energy photon beam. However, in this study, the measurement uncertainty of GD-352M was analyzed for evaluating the protection quantities. The measurement uncertainty factors in the RPLGD include the reference irradiation, regression curve, reproducibility, uniformity, energy dependence, and angular dependence, as described by the International Organization for Standardization (ISO). These factors were calculated using the Guide to the Expression of Uncertainty in Measurement method, applying ISO/ASTM standards 51261(2013), 51707(2015), and SS-ISO 22127(2019). The measurement uncertainties of the RPLGD reader system with a coverage factor of k = 2 were calculated to be 9.26% from 0.005 to 1 Gy and 8.16% from 1 to 10 Gy. A blind test was conducted to validate the RPLGD reader system, which demonstrated that the readout doses included blind doses of 0.1, 1, 2, and 5 Gy. Overall, the E_n values were considered satisfactory.

• International Journal of Computer Science & Network Security
• /
• 제22권10호
• /
• pp.183-190
• /
• 2022

The article analyzes the features of the formation of a person's value attitude to the worldview by means of information technologies. The present considers it necessary to form a person's value attitude to the perception of the world by means of information technologies. The explosive development of information and telecommunications technologies has become a determining factor in the development of modern society, which is called the information or Global Information Society. It is not yet fully formed, and we are all participants in the development of the Global Information Society. The article considers the basics of a harmonious worldview of a person, which is the basis for the formation of outlook ideas, views, knowledge, beliefs about the surrounding world, which determine the place and role and motivate actions in relation to the surrounding reality through the prism of value orientations. Worldview is considered as an integrity of relatively stable schemes, behaviors, feelings, thinking, vision of the surrounding world, inherent in an individual child, ethno-cultural and socio-cultural groups. The concept of "worldview" as a component of the multi-level structure of the individual's outlook is defined. The features that characterize a person's perception of the world are revealed. The main educational value of information technologies in the formation of a person's value attitude to the perception of the world is highlighted, which consists in the fact that they allow you to create an immeasurable brighter multi-sensory interactive learning environment with almost unlimited potential opportunities that fall at the disposal of both the teacher and the student. The trend of forming a person's value attitude to the perception of the world is clearly developing in the direction of mixed learning as a process that creates a comfortable information educational environment, communication systems that provide all the necessary educational information. The approach to student development by means of the educational environment and the formation, while in the person of a value attitude to the perception of the world by means of Information Technologies, has many pedagogical advantages, which is considered in the article.

• Journal of Animal Science and Technology
• /
• 제64권3호
• /
• pp.481-499
• /
• 2022

This study aims to determine the effects of D-methionine (D-Met) isomer and the methionine precursor 2-hydroxy-4-methylthiobutanoic acid i (HMBi) supplementation on milk protein synthesis on immortalized bovine mammary epithelial cell (MAC-T). MAC-T cells were seeded using 10-cm dishes and cultured in Dulbecco's modified Eagle's medium/F12 (DMEM/F12) basic medium. The basic medium of DMEM/F12 was replaced with the lactogenic DMEM/ F12 differentiation medium when 90% of MAC-T cells reached confluency. The best dosage at 0.6 mM of D-Met and HMBi and incubation time at 72 h were used uniformly for all treatments. Each treatment was replicated six times wherein treatments were randomly assigned in a 6-well plate. Cell, medium, and total protein were determined using a bicinchoninic acid protein assay kit. Genes, proteomics and metabolomics analyses were also done to determine the mechanism of the milk protein synthesis pathway. Data were analyzed by two-way analysis of variance (ANOVA) with supplement type and plate as fixed effects. The least significant difference test was used to evaluate the differences among treatments. The HMBi treatment group had the highest beta-casein and S6 kinase beta-1 (S6K1) mRNA gene expression levels. HMBi and D-Met treatments have higher gene expressions compared to the control group. In terms of medium protein content, HMBi had a higher medium protein quantity than the control although not significantly different from the D-Met group. HMBi supplementation stimulated the production of eukaryotic translation initiation factor 3 subunit protein essential for protein translation initiation resulting in higher medium protein synthesis in the HMBi group than in the control group. The protein pathway analysis results showed that the D-Met group stimulated fructose-galactose metabolism, glycolysis pathway, phosphoinositide 3 kinase, and pyruvate metabolism. The HMBi group stimulated the pentose phosphate and glycolysis pathways. Metabolite analysis revealed that the D-Met treatment group increased seven metabolites and decreased uridine monophosphate (UMP) production. HMBi supplementation increased the production of three metabolites and decreased UMP and N-acetyl-L-glutamate production. Taken together, D-Met and HMBi supplementation are effective in stimulating milk protein synthesis in MAC-T cells by genes, proteins, and metabolites stimulation linked to milk protein synthesis.

• Fisheries and Aquatic Sciences
• /
• 제26권3호
• /
• pp.204-215
• /
• 2023

The thermolabile haemolysin (tlh) of Vibrio parahaemolyticus (Vptlh) from V. parahaemolyticus is a multiple-function enzyme, initially describes as a haemolytic factor activated by lecithin and phospholipase A2 enzymatic activity (Shinoda, 1991; Vazquez-Morado, 2021; Yanagase et al., 1970). Until now, the tlh structure has hypothesized including N-terminal and C-terminal domain, but what domain of the Vptlh structure does the haemolytic activity has not been refined yet. In this study, a 450-bp VpTLH nucleotide sequence of the entire Vptlh gene encoded the C-terminal domain cloned firstly to examine its responsibility in the activity of the Vptlh. The C-terminal domain fused with a 6-His-tag named the His-tag-VpC-terminal domain was expressed successfully in soluble form in the BL21 (DE3) PlysS cell. Remarkably, both expression and purification results confirmed a high agreement in the molecular weight of the His-tag-VpC-terminal domain was 47 kDa. This work showed the His-tag-VpC-terminal domain lysed the erythrocyte membranes in the blood agar and the phosphate buffered saline (0.9%) media without adding the lecithin substrate of the phospholipase enzyme. Haemolysis occurred at all tested diluted concentrations of His-tag-VpC-terminal domain (p < 0.05), providing evidence for the independent haemolytic activity of the His-tag-VpC-terminal domain. The content of 100 ㎍ of the His-tag-VpC-terminal domain brought the highest haemolytic activity of 80% compared to that in the three remaining contents. Significantly, the His-tag-VpC-terminal domain demonstrated not to involve the phospholipase activity in Luria-Bertani agar supplemented with 1% (vol/vol) egg yolk emulsion. All results proved the vital responsibility of the His-tag-VpC-terminal domain in causing the haemolytic activity without the required activation by the phospholipase enzyme. Raw extracts of Phellinus igniarus and Phellinus pipi at 10^-1 mg/mL inhibited the haemolytic activity of the His-tag-VpC-terminal domain from 67.7% to 87.42%, respectively. Hence applying the His-tag-VpC-terminal domain as a simple biological material to evaluate quickly potential derivatives against the Vptlh in vivo conditions will accessible and more advantageous than using the whole of the Vptlh.

• Animal Bioscience
• /
• 제36권8호
• /
• pp.1228-1240
• /
• 2023

Objective: This experiment was conducted to evaluate the effects of different levels of dietary crude protein (CP) on growth performance, blood profiles, diarrhea incidence, nutrient digestibility, and odor emission in weaning pigs. Methods: A total of 240 weaning ([Yorkshire×Landrace]×Duroc) pigs (8.25±0.050 kg body weight [BW]) were assigned to six treatments based on sex and initial BW, with five replicates of eight pigs per pen in a randomized complete block design. Experimental diets with different crude protein levels for early and late weaning phases were as follows: i) CP16, corn-soybean-based diet containing 16%/15% CP; ii) CP17, corn-soybean-based diet containing 17%/16% CP; iii) CP18, corn-soybean-based diet containing 18%/17% CP; iv) CP19, corn-soybean-based diet containing 19%/18% CP; v) CP20, corn-soybean-based diet containing 20%/19% CP; and vi) CP21, corn-soybean-based diet containing 21%/20% CP. Results: In the early weaning period, average daily feed intake increased when the dietary CP level decreased (linear, p<0.05). During the entire experimental period, average daily gain and the gain to feed ratio decreased when the dietary CP level increased (linear, p<0.01). Additionally, a decrease in dietary CP level resulted in a linear increase in final BW (linear, p<0.05). In the early and late weaning periods, blood urea nitrogen (BUN) decreased when the dietary CP level decreased (linear, p<0.01). There were no significant differences in creatinine, glucose, total protein, triglyceride or insulin-like factor-1 levels over the experimental period. The concentrations of immunoglobulin A (IgA) and IgG were not significantly affected by dietary CP levels during the experimental period. In the early weaning period, fecal and urine N decreased when the dietary CP level decreased (linear, p<0.01). No differences in nutrient digestibility among the treatments during the early weaning period were found. Throughout the whole experimental period, when the dietary CP level decreased in the weaning pig diet, the diarrhea incidence decreased linearly (linear, p<0.01). Throughout the whole experimental period, when the dietary CP level decreased in the weaning pig diet, ammonia, amines and hydrogen sulfide decreased linearly (linear, p<0.01). Conclusion: Reducing dietary CP could decrease diarrhea incidence, the concentration of BUN in serum and odor emission in manure. Furthermore, it could improve N excretion in feces and urine and growth performance in weaning pigs.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2022년도 추계학술대회
• /
• pp.202-202
• /
• 2022

Recently days, soybean production in paddy field is increasing, from 4,422 ha in 2016 to 10,658 ha in 2021 in Korea. It is easy for Phytophthora stem and root rot (PSR) occurring in paddy field condition, when it is poorly drained soils with a high clay content, and temporary flooding and ponding. Therefore PSR resistant soybean cultivar is required. The objective of this study is to identify QTL region and candidate genes relating to PSR resistance of the race in main soybean cultivation area in Korea. 210 soybean materials including cultivars and germplasm were used for inoculation and genome-wide association study (GWAS). Inoculation was conducted using stem-scar method with 2 replications in 2-year for the race 3053 from Kimje and 3617 from Andong. 210 materials were genotyped with Soya SNP 180K chip, and structure analysis and association mapping were conducted with QTLMAX V2. The results of inoculation showed that survival ratio ranged from 0% to 96.7% and mean 9.7% for 3053 and ranged from 0% to 100% and mean 7.6% for 3617. Structure analysis showed linkage disequillibrium (LD) was decayed below r²=0.5 at 335kb of SNP distance. Significant SNPs (LOD>7.0) were identified in Chr 1, 2, 3, 4, 5, 11, 14, 15 for 3053 and Chr 1, 2, 3, 7, 10, 14 for 3617. Especially, LD blocks (AX-90455181;15,056,628bp~AX-90475572;15,298,872bp) in Chr 2 for 3053 and 3067 were duplicated. 29 genes were identified on these genetic regions including Glyma.02gl47000 relating to ribosome recycling factor and defense response to fungus in Soybase.