• Bulletin of the Korean Chemical Society
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• 제24권8호
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• pp.1163-1168
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• 2003

The structures of molecular species of galactolipids, such as monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG), isolated from wheat flour have been investigated using negative-ion electrospray ionization (ESI) mass spectrometry interfaced with high performance liquid chromatography (HPLC). According to the result of HPLC analysis, MGDG and DGDG were found to consist of mixtures of five and four molecular species, respectively. The galactolipids have been also analyzed to determine their fatty acid compositions, using HPLC/ESI-MS combined with in-source (or cone voltage) fragmentation. HPLC/ ESI-MS is very useful for one-step analysis of mixtures of galactolipids with a small sample quantity. Especially, the carboxylate anions produced in in-source fragmentations of the negative-ion of each component separated by HPLC provide valuable information on the composition of its fatty acyl chains.

• BMB Reports
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• 제44권8호
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• pp.517-522
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• 2011

Mitochondrial dynamics not only involves mitochondrial morphology but also mitochondrial biogenesis, mitochondrial distribution, and cell death. To identify specific regulators to mitochondria dynamics, we screened a chemical library and identified niclosamide as a potent inducer of mitochondria fission. Niclosamide promoted mitochondrial fragmentation but this was blocked by down-regulation of Drp1. Niclosamide treatment resulted in the disruption of mitochondria membrane potential and reduction of ATP levels. Moreover, niclosamide led to apoptotic cell death by caspase-3 activation. Interestingly, niclosamide also increased autophagic activity. Inhibition of autophagy suppressed niclosamide-induced cell death. Therefore, our findings suggest that niclosamide induces mitochondria fragmentation and may contribute to apoptotic and autophagic cell death.

• 화약ㆍ발파
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• 제17권2호
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• pp.19-35
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• 1999

Rock fragmentation with plasma blasting technique has advantageous properties in contrast to the conventional blasting method controlling of flying rocks and ground vibrations, when residents are complaining or surrounding structures stay in protection from blasting operations. The experiences show in urban construction works that the plasma blasting is the most possible method to prevent damages and minimize adverse environmental impacts. The fragmentation energy level is evaluated by numerical simulation using PFC for various drill hole patterns and tested accordingly to get the feasibility. The energy output of plasma blasting system has been improved to a level of 1 MJ, which can break a 2-3 ㎥ granite boulder or 1.5m height bench face. Measurements are carried out to get the ground vibration level and propagation equation, so that control of the blasting operations can be performed more precisely and safely.

• 화약ㆍ발파
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• 제23권3호
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• pp.19-25
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• 2005

본 연구에서는 이완식 발파공법과 건교부 제시 발파공법을 적용한 현장에서 지반진동과 소음을 측정, 분석하고 비교하였다. 건교부 방식 발파에 비해 이완식 발파공법이 다소간 진동감쇠 효과가 있었다 발파매트를 사용한 이완식 발파시험 견과가 건교부 방식 발파에 비하여 소음이 감쇠되는 것으로 나타났으며, 파쇄입도는 다소 떨어졌다.

• 약학회지
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• 제46권1호
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• pp.69-74
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• 2002

The effects of genistein on murine thymocytes for inducing apoptotic cell death and phagocytic activity of peritoneal macrophage were studied in vitro. Addition of genistein (10 and 50$\mu$M) to cultured thymocytes from BALB/c mice definitely promoted DNA fragmentation. Also, cytofluorometric analysis of these cells demonstrated a reduction in mitochondrial transmembrane potential ($\Delta$Ψm). But, repeated administration of genistein (1 mg/mouse/day) to mice for 7 days did not cause any detectable DNA fragmentation. Genistein decreased lucigenin chemiluminescence and engulfment of fluorescein-conjugated E. coli particles in peritoneal macrophage. These results suggest that genistein induce an apoptosis of thymocyte via reduction in $\Delta$Ψm and decrease phagocytic activity of peritoneal macrophage in vitro.

• 천문학회보
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• 제41권2호
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• pp.46.4-47
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• 2016

About 50% of stars reside in binary or multiple systems. However, the formation mechanism of the multiplicity is poorly understood. Theoretical studies suggest two main mechanisms for the multiplicity: turbulent fragmentation and disk fragmentation. We can testify which mechanism is more plausible by measuring the separation between companions or the alignment of stellar spins. Here we present our ALMA Cycle 2 observational results of a proto-binary system, IRAS 04191+1523, which consists of two Class I sources. We detected disks around both Class I sources, which are located in a common dense filamentary structure traced by $C^{18}O$ J=2-1. Two protostellar disks are separated by ~900 AU and their rotational axes are almost perpendicular, which strongly support that this binary system formed by the turbulent fragmentation.

• 한국환경성돌연변이발암원학회지
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• 제19권1호
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• pp.34-38
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• 1999

Fumonisin B1 is a secondary metabolite of Fusarium moniliforme, a contaminant of corn and corn product. Fumonisin B1 has been shown to be responsible for major toxicological effects of the fungus in rats, horses, and pigs. Fumonisin B1 induced λ DNA fragmentation, which was increased with incubation time, reducing agent NADPH and metal ion (Cu2+). The DNA damage was inhibited by dimethyl sulfoxide (DMSO) or mannitol as radical scavenger for free radicals. DNA fragmentation, induced by fumonisin B1 in the presence of 1 mM NADPH and 0.1 mM CuCl2, was inhibited by 100 mM DMSO. By the in vitro reaction of fumonisin B1 with supercoiled plasmid pBR322 DNA, plasmid DNA was relaxed, eventually linearized in the agarose gel electrphoresis. From rifampicin sensitive E. coli CSH138 in bacterial mutagenesis system, the rifampicin resistant E. coli mutants were obtained by fumonisin B1. These results suggest that fumonisin B1 may be a possible environmental mutagen in bacterial mutagen assay system.

• Toxicological Research
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• 제13권3호
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• pp.187-191
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• 1997

Cell death induced by polychlorinated biphenyls (PCBs), environmental toxicant, was investigated in mouse splenocytes. The fragmentation of intact DNA, a parameter of apoptotic cell death, was evaluated qualitatively by agarose gel electrophoresis analysis and quantitatively by diphenylamine reaction method. PCBs induced apoptotic cell death of splenocytes in a dose- and time- dependent manner. The effect of serum on the apoptotic cell death induced by PCBs was also investigated. The DNA fragmentation induced by PCB treatment in serum-free medium was clearly inhibited by an addition of serum to the culture medium. The decrease of DNA fragmentation due to serum addition was accompanied with the increase of cell viability.

• Environmental Analysis Health and Toxicology
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• 제19권3호
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• pp.251-259
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• 2004

Xenoestrogens are chemicals with diverse structure that mimic estrogen. Bisphenol A, a monomer of polycarbonate and epoxy resins, has been detected in canned food and human saliva. Bisphenol A stimulate cell proliferation and induce expression of estrogen -response genes in vitro. The purpose of the this study was to evaluate cell proliferation of bisphenol A in the presence of a rat liver 59 mix contaning cytochrome P450 enzymes and Cu (II). The fragmentation of intact DNA, a parameter of apoptotic cell death, was evaluated quantitatively by diphenylamine reaction method. Bisphenol A induced apoptotic cell death in a dose-dependent manner The effect of radical scavenger on the apoptotic cell death induced bisphenol A was investigated. The DNA fragmentation induced by bisphenol A was significantly inhibited by addition of radical scavenger to the culture medium. This indicated that elevated oxidative stress caused by imbalance between the production and removal of free radicals occurred in cells. Taken together, these results suggest that free radical reacts with Cu (II) leading oxidative stress.

• 화약ㆍ발파
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• 제17권1호
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• pp.19-26
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• 1999

Rock fragmentation with plasma blasting technique has advantageous properties in contrast to the conventional blasting method in controlling of flying rocks and ground vibrations when residents are complaining or surrounding structures stay in protection from blasting operations. The experiences show in urban construction works that the plasma blasting is the most possible method to prevent damages and minimize adverse environmental impacts. The fragmentation energy level is evaluated by numerical simulation using PFC for various drill hole pattern and tested accordingly to get the feasibility. The energy output of plasma blasting system has been improved to a level of 1 MJ, which can break a $2-3m^3$ granite boulder or 1.5m height bench face. Measurements are carried out to get the ground vibration level and propagation equation. So that the control of the blasting operations can be performed more precisely and safely.