• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.2
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• pp.335-341
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• 2000

In order to search for blood anticoagulant substance from herbal extracts, we extracted with cold water (Fr. I), methanol (Fr. II) and hot water (Fr. III) from 93 commercially available herbs. The anticoagulant activity of herbal extracts was examined through the intrinsic pathway by activated partial thromboplastin time (APTT), the extriansic pathway by prothrombin time (PT) and the common pathway by thrombin time (TT). Onion, garlic, clove, fenugreek, mugwort, thyme and sage had on anticoagulant activity with intrinsic pathway and mint, italian seasoning, rosemary, turmeric, tarragon and wasabie had on anticoagulant activity with common pathway. Nobody had on anticogulant activity with extrinsic pathway. Among commerical herbs tested, clove cultivated in holland was selected because it showed the most potent anticoagulant activity among the samples investigated.

• Archives of Pharmacal Research
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• v.20 no.1
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• pp.13-16
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• 1997

The cytotoxicity of animal venoms (snakes, insects and marine animals) was measured against SNU-1 (stomach cancer cells) by dye uptake assay (MTT method). And also L-amino acid oxidase (AAO) activity of the venoms was compared. Among them, the venom from Ophiophagus hannah (king cobra) showed a strong AAO activity as well as a high potent cytotoxicity. Cytotoxic protein having a AAO was then partially purified by HPLC-GPC and two fractions (Fr. I and Fr. II) were collected. The $IC_{50}$ values of Fr. I and Fr. II were 0.19 ${\mu}g/ml$ and 1.36 ${\mu}g/ml$, respectively. The results suggested that the cytotoxicity of king cobra venom may be due to its AAO activity.

• The Bulletin of The Korean Astronomical Society
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• v.46 no.2
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• pp.39.2-39.2
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• 2021

To investigate the acceleration of ultra-high energy cosmic rays (UHECRs) in relativistic jets of FR-II galaxies, we simulate high-power jets with jet powers of Q~10^46erg/s in a stratified galaxy cluster halo using a state-of-art relativistic hydrodynamic (RHD) code we have recently developed. With the simulated jet-induced flow profiles, we then perform Monte-Carlo simulations, where the transport of high-energy particles is followed assuming large-angle scatterings in the flow-rest frame. We estimate the energy gains and acceleration times in the acceleration processes by shocks, shear, and turbulence. We present the results and discuss implications on the acceleration of UHECRs in FR II radio jets.

• Journal of Ginseng Research
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• v.40 no.3
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• pp.237-244
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• 2016

Background: Ginsenoside Rg3 and arginine-fructose (Arg-Fru) are known as the hypotensive compounds of Panax ginseng; however, their efficacy on antihypertension has not been reported yet to our best knowledge. Thus, hypotensive components-enriched fraction of red ginseng (HCEF-RG) was prepared from fine root concentrate (FR) and their antihypertensive effects were investigated in spontaneously hypertensive rats (SHR). Methods: Male SHRs were divided into six groups: control (Wistar Kyoto, SHR); FR 500; FR 1,000; HCEF-RG 500; and HCEF-RG 1,000; samples (mg/kg body weight) were orally administered every day for 8 wk. Blood pressure was monitored at 1 wk, 2 wk, 3 wk, 4 wk, 6 wk, and 8 wk by tail cuff method. At 8 wk after samples administration, mice were killed for the measurement of renin activity (RA), angiotensin-I converting enzyme inhibition, angiotensin II, and nitric oxide (NO) levels in plasma. Results: HCEF-RG with four-fold more Rg3 and 24-fold more Arg-Fru contents was successfully prepared from reacted mixtures of FR and persimmon vinegar (12 times against FR, v/v) at $80^{\circ}C$ for 18 h. Both FR 1,000 and HCEF-RG 1,000 showed lowered systolic blood pressure than SHR control group and HCEF-RG 1,000 group exhibited a significant decrease in diastolic blood pressure. RA was significantly lowered in all treated groups, while angiotensin II did not affect by FR and HCEF-RG treatment. However, angiotensin-I converting enzyme inhibition and NO in FR 1,000 and HCEF-RG 1,000 were significantly increased compared with SHR control group. Conclusion: HCEF-RG is more effective and useful for alleviating hypertension than FR, implying the health benefit of Rg3 and Arg-Fru.

• Journal of Microbiology and Biotechnology
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• v.18 no.3
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• pp.512-519
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• 2008

Three different polysaccharide-peptide complexes (PPC, named as Fr-I, Fr-II, and Fr-III) were produced by submerged mycelial culture of an entomopathogenic fungus Cordyceps sphecocephala, and their anticancer activities were investigated in human hepatocarcinoma (HepG2) and neuroblastoma (SK-N-SH) cells. The highest inhibitory effects of PPC on both HepG2 and SK-N-SH cells were achieved with Fr-I, whereas Fr-III with low molecular mass showed lower inhibition effects. Interestingly, the inhibitory effects of the three fractions were increased after protease digestion, suggesting that the inhibitory effects resulted mainly from the carbohydrate moiety, at least in the case of Fr-II and Fr-III, of PPC. The results of DNA fragmentation in PPC-induced apoptotic cells were confirmed by both DNA ladder assay and comet assay. Our investigation also showed that PPC-induced apoptosis of both cancer cells was associated with intracellular events including DNA fragmentation, activation of caspase-3, and modulation of Bcl-2 and Bax. We conclude that PPC has potential as a novel therapeutic agent for the treatment of both HepG2 and SK-N-SH cancer cells without any cytotoxicity against normal cells.

• Journal of the Korean Home Economics Association
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• v.32 no.1
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• pp.59-74
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• 1994

Den Ausgangspunkt fr die Untersuchungen der vorliegenden Studie(II) bildeten die Auswirkungen des Rollenwandels auf das elterliche Verhalten und die Beziehung der Eltern zueinander. Im Rahmen dieser Studie beschrnkt sich die Untersuchung auf eine bestimmte Phase des Familienlebens nmlich den Ubergang zur Elternschaft und fr diesen Bereich wurden Angaben zu Arveiten im Haushalt mit Zufriedenheit. Beurteilt wurden Ubergangseffekte im Vergleich von Vernderungen bei Erst-und Zweiteltern die im Zusammenhang mit dem Baby aufgetreten sind. Disses Vorgehen basierte auf der Annahme da$\beta$ bei Ersteltern strkere Effekte auftreten als bei Zweiteltern, Die Ergebnisse basieren auf 130 sdkoreanischen Erst-bzw. Zweiteltern die whrend der Schwangerschaft(ca 6 Monat) erstmals befragt wurden und auf vervliebenen 89 Elternpaaren die sich an einer zweiten Befragung drei Monate nach der Geburt des Babys beteiligten. Die Ergebnisse zur Aufteilung von Haushaltsarbeiten haben fr die Gesamtstichprobe gezeigt da$\beta$in der Zeit vor und nach der Geburt das Kindes die Arbeiten im Haushalt meistens von den Mttern bernommen wurden. Beim Vergleich von Erst-und Zweiteltern wurden Unterschiede in der Aufteilung der Aebeiten im Huashalt gefunden. Diese Unterschiede besthen insbesondere darin da$\beta$ die Zweitmtter(vor der Geburt des zweites Kindes) noch mohr Haushaltsarbeiten die Erstmtter( der Geburt des zweites Kindes) bernehmen. Bezglich der Zufriedenheit mit der Aufteilung der Arbeiten im Haushalt wurden bei den Vtern Unterschiede drei Monate nach der Geburt des Kindes gefunden. Nach der Geburt des Kindes sind die meisten Zweitvter zufriedener mit ihrer Rolle im Haushalt als die Erstvter nach der Geburt ihres Kindes.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.10
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• pp.1545-1549
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• 2003

Broiler chickens were subjected to (i) pre-treatment for 24 h with 1,200 ppm L-ascorbic acid in their drinking water on day 42 (AA), (ii) 60% feed restriction on days 4, 5 and 6 (FR), (iii) combination of AA and FR (AAFR), or (iv) neither treatment (control). On day 43, the birds were crated and transported in an open truck for either 60 min or 120 min. Heterophil/lymphocyte ratios (HLR) were determined prior to ($T_0$) and 20 h following the 60 min ($T_60$) and 120 min ($T_120$) journeys. Prior to transportation, all treatment groups had similar HLR. Following $T_60$, the AAFR chicks had smaller increase in HLR than their control, AA and FR counterparts. The AA, FR and AAFR birds exhibited lower HLR than controls following $T_120$. The means HLR of AA, FR and AAFR did not differ significantly. Irrespective of journey time, AA and AAFR birds had shorter tonic immobility duration than their FR and control counterparts. The tonic immobility duration of all birds increased with journey duration.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.253-253
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• 1994

영지와 구름버섯의 원형질체 융합 균주 F-1의 배양 균사체로 부터 열수추출한 분획 Fr.I을 DEAE-cellulose ion chromatography와 gel filtration chromatography를 통하여 분획 Fr.II, III, IV,로 분리 정제하였다. Sarcoma 180에 대한 종양억제율을 검사한 결과 Fr.IV는 68.73%로 가장 우수하였으며 동계 복수암에 대한 수명 연장 효과도 140 %의 유의적인 결과를 얻었다. 면역 관련 장기의 중량에 대한 영향을 실험한 결과 정상군에 비해 간, 비장 및 흉선의 중량을 증가시켰고, 마우스 암세포에 대한 직접적인 세포독성 작용을 보이지 않았으나 면역 실험을 실시한 결과, 마우스에서 용혈반 형성세포수를 1,36배 증가시켰으며 암이식군에서 감소된 T lymphocyte활성을 정상수준까지 회복시켰고 또, macrophage의 superoxide anion 분비를 2.25배 증가시켰다. 이로써 이 항암 성분은 면역세포를 활성화시켜 항암 효과를 나타냄을 의미한다. Fr.IV히 분자량은 7.9$\times$$10^4$ dalton이고 75,57%의 다당체와 4.47%의 단백질로 이루어졌으며 그 다당체는 주로 glucase, xylose와 mannose로 구성된 heteropolysaccharide이었으며 그 단백질은 Alanine과 Valine을 위시한 15종의 아미노산을 함유하고 있음이 확인되었다.

• YAKHAK HOEJI
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• v.55 no.3
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• pp.227-233
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• 2011

We previously reported the protein isolated from Coptidis Rhizoma (CRP), which has antifungal activity against a fungal pathogen, Candida albicans. In the current study, we investigated what portion in the CRP is responsible for the antifungal activity. For the investigation, the CRP was fractionated on a Shepadex G-50 column. Data resulting from the fractionation, seven fractions were obtained. Fractions (Fr.) I, II, and III eluted initially from the column showed no inhibitory effect on the growth of C. albicans, whereas Fr. IV, V, and VI eluted later revealed inhibition of the growth, and Fr. IV and VI showed potent antifungal activity by broth susceptibility analysis. However, Fr. VI was contained in the CRP more than Fr. IV, which led us to select the VI for the following experiments. In a murine model of a subcutaneous candidiasis caused by C. albicans, the Fr. VI displayed a therapeutic effect on nude mice pretreated with anti-neutrophil monoclonal antibody (RB68C5) and then infected subcutaneously with live C. albicans. At day 16, these mice were healed almost up to 78% of the infected area when compared to infected area of control nude mice that received diluent (Dulbecco's Phosphate-Buffered Saline; DPBS), instead of the Fr. VI (P<0.01). The Fr. VI blocked hyphal formation from blastoconidial form of C. albicans (P<0.01), which might prevent penetration of hyphae to the deeper site of skin and thus helps the healing. In the ionic strength test, the effect of Fr. was influenced by $Ca^{2+}$ ion just like other known antimicrobial peptides, but the influence was affected at an extremely high concentration such as 500 mM. Thus, such ion-concentration is considered to be meaningless in the clinical situation. Considering all data together, Coptidis Rhizoma is appeared to produce an antimicrobial peptide that has therapeutic effect on subcutaneous infection caused by C. albicans.

• Physical Therapy Korea
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• v.12 no.2
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• pp.58-72
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• 2005

The purposes of this study were to determine the effects of rehabilitation programs on functional performance of the lower extremities and whether additional therapeutic exercise with routine rehabilitative therapy improves functional performance more than just routine rehabilitative therapy by it self for inpatients who have suffered a stroke with below moderate severity within 3 to 6 months after the onset of the stroke. Fifty-eight subjects were divided into two groups. Group I was given routine rehabilitative therapy and group II was given additional therapeutic exercise along with the routine rehabilitative therapy. Each group received 6 weeks of rehabilitation. The timed get-up and go test (TUG), the Fugl-Meyer score (FMS), functional independence measure (FIM), functional reach (FR), gait velocity (GV), and the strength of knee extensor and flexor were selected to measure effect of rehabilitation programs. The main results were measured and analysed at baseline, 3 weeks, and 6 weeks after the start of the rehabilitation programs. The results revealed that all of functional performance of the lower extremities in 3 weeks after the start of the rehabilitation programs were significantly improved compared with before the rehabilitation programs in both groups. In 6 weeks, TUG, FIM, FR, GV, and the strength of knee extensor in group I, TUG, FMS, FIM, FR, GV, and the strength of knee extensor and flexor in group II were significantly improved compared with the results after 3 weeks. At 3 weeks after rehabilitation programs, group II made significantly greater gains in TUG, FR, GV, and the strength of the knee flexor compared to the group I. At 6 weeks, group II made significantly greater gains in TUG, FR, GV, and the strength of the knee extensor and flexor compared to the group I. In conclusion, rehabilitation programs for stroke patients within 3 to 6 months after stroke onset significantly contributed to improve functional performance of the lower extremities. It is desirable for improvement of functional performance of the lower extremities to perform additional exercise with routine rehabilitative therapy.