• Journal of Dental Anesthesia and Pain Medicine
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• 제16권1호
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• pp.39-47
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• 2016

Background: Oxidative stress occurs during the aging process and other conditions such as bone fracture, bone diseases, and osteoporosis, but the role of oxidative stress in bone remodeling is unknown. Propofol exerts antioxidant effects, but the mechanisms of propofol preconditioning on oxidative stress have not been fully explained. Therefore, the aim of this study was to evaluate the protective effects of propofol against $H_2O_2$-induced oxidative stress on a human fetal osteoblast (hFOB) cell line via activation of autophagy. Methods: Cells were randomly divided into the following groups: control cells were incubated in normoxia (5% $CO_2$, 21% $O_2$, and 74% $N_2$) without propofol. Hydrogen peroxide ($H_2O_2$) group cells were exposed to $H_2O_2\;(200{\mu}M)$ for 2 h, propofol preconditioning (PPC)/$H_2O_2$ group cells were pretreated with propofol then exposed to $H_2O_2$, 3-methyladenine (3-MA)/PPC/$H_2O_2$ cells were pretreated with 3-MA (1 mM) and propofol, then were exposed to $H_2O_2$. Cell viability and apoptosis were evaluated. Osteoblast maturation was determined by assaying bone nodular mineralization. Expression levels of bone related proteins were determined by western blot. Results: Cell viability and bone nodular mineralization were decreased significantly by $H_2O_2$, and this effect was rescued by propofol preconditioning. Propofol preconditioning effectively decreased $H_2O_2$-induced hFOB cell apoptosis. However, pretreatment with 3-MA inhibited the protective effect of propofol. In western blot analysis, propofol preconditioning increased protein levels of collagen type I, BMP-2, osterix, and TGF-${\beta}1$. Conclusions: This study suggests that propofol preconditioning has a protective effect on $H_2O_2$-induced hFOB cell death, which is mediated by autophagy activation.

• 한국산학기술학회:학술대회논문집
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• 한국산학기술학회 2011년도 춘계학술논문집 1부
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• pp.101-104
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• 2011

본 논문에서는 FOB Key Smart Key System module 간의 원할한 통신을 위한 실차 테스트를 하였으며, 실차 테스트를 위한 데모 모듈레이션 구성은 물론 각 모듈간의 통신상태및 최적의 통신 상태를 위한 3차원 그리드 형태의 실험준비를 하였고 최적의 감도를 얻기위한 수천번의 실차 테스트를 실시하였다.

• 대한가정학회지
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• 제36권2호
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• pp.161-173
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• 1998

This study investigates why the Married Female Production Workers(MFPW) participate in their work and what is work load they take. This study uses an ecosystems approaches in order to explain the labor participation of the MFPW and their work load. MFPW participate in their work because of the labor markets need and economic need of their household rather than their personal choice. Due to the long working hours, low wage and bad labor conditions of MFPW's job, they suffer more severe work load both at the fob and at home than any other occupational groups.

• 한국정보통신학회논문지
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• 제19권12호
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• pp.2993-3002
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• 2015

국제 물품매매계약에서 INCOTERMS 2010 이 적용되기 시작한 지 5년이 지나면서 그 사용현황과 실무적 적용의 문제점을 분석하기 위하여 지난 10년간 우리나라 수출 거래계약조건과 운송서류 발행 형태를 조사하였다. 조사 결과 첫째, INCOTERMS 2010은 공식 규칙이 아닌 몇 가지 규칙이 사용되고 있으며, 둘째, 해상 및 내수로 운송에 사용되어야 하는 전통적 사용 규칙인 FAS, FOB, CIF, CFR 규칙 사용이 여전히 대부분을 차지하며 모든 운송에 사용되는 규칙으로 변경되지 않고 있다. 셋째, CPT, CIP조건에서 물품의 인도시기 즉 소유권이전의 문제가 이 두 조건의 사용을 활성화 시키지 못하는 실질적 이유가 되고 있다. 넷째, DAT 조건은 사용이 극히 저조 한데 그 이유는 터미널의 장소를 지정이 실무적으로 계약 시점에 확정하기 어렵고 운송 과정에 변경되기도 한다. 이러한 문제점들은 INCOTERMS 2010에 대한 적절한 사용 조건에 대한 보다 적극적인홍보 활동이 필요하며, 거래조건을 해석하고 분쟁 해결을 위한 내용을 서문에 제시하는 것이 바람직 할 것이다.

• 무역상무연구
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• 제49권
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• pp.3-41
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• 2011

In this article, the author have studied on main revisions and some recommendations of the Incoterms(R) 2010. Main revisions are as belows. 1. Two new Incoterms rules -DAT and DAP- have replaced the Incoterms 2000 rules DAF, DES, DEQ and DDU. 2. New classification of the Incoterms(R) 2010 are adopted. First class is Rules for any mode or modes of transport(EXW, FCA, CPT, CIP, DAT, DAP and DDP belong to this class.) and second class is rules for sea and inland waterway transport(FAS, FOB, CFR and CIF belong to this class.). 3. Incoterms(R) 2010 rules formally recognizes that they are available for application to both international and domestic sale contracts. 4. The Guidance Notes and Introduction are not part of the actual Incoterms(R) 2010 rules. 5. Under the FOB, CFR and CIF, all mention of the ship's rail as the point of delivery has been omitted in preference for the goods being delivered when they are "on board" the vessel. 6. Incoterms(R) 2010 rules include the obligation to 'procure goods shipped' as an alternative to the obligation to ship goods in the relevant Incoterms rules. 7. Incoterms(R) 2010 rules give electronic means of communication the same effect as paper communication. 8. Incoterms(R) 2010 rules have allocated obligations between the buyer and seller to obtain or to render assistance in obtaining security-related clearances. such as chain-of custody information. Some recommendations are as belows. 1. The parties must incorporate the Incoterms(R) 2010 rules into their contract of sale. 2. The parties must choose the appropriate Incoterms(R) 2010 rules. 3. Specify the place or port as precisely as possible in their contract of sale. 4. Remember that Incoterms(R) 2010 rules do not give the parties a complete contract of sale. 5. Incoterms(R) 2010 rules do not prohibit alteration of Incoterms rule, but there are dangers in so doings. In order to avoid any unwelcome surprises, the parties would need to make the intended effect of such alterations extremely clear in their contract.

• 무역상무연구
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• 제43권
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• pp.53-74
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• 2009

INCOTERMS have been changed almost every 10 years since ICC established INCOTERMS as trade terms for International transaction. Recently transportation has become bigger, and modernized by means of electronic appliances such as RFID, IT, and containerization. FRC, FOR/FOT, FOA were added in INCOTERMS1980 and every conditions are unified into three alphabets in INCOTERMS1990. The best features of INCOTERMS2000 are that FCA substitute FRC, FOR/FOT, FOA and customs formalities were simplified to make clear for each party of contract. It seems that business circles still stick to old customs of their trade like FOB or CIF not only in Korea but in an international practice even though there have been several revisions of INCOTERMS until now. ICC have tried to provide INCOTERMS3000 to solve problems between a theory and an actual condition of international trade. This study has tried to suggest opinions against INCOTERMS3000 and has surveyed a recognition, an actual using situation and issues of INCOTERMS to get improvements. For a recognition of INCOTERMS has been spread as you can find at tables many kinds of business circles still stick to old customs of their trade terms FOB and CIF. Now there are two alternative plans. Firstly, we need to suggest improvements against inconsistency of INCOTERMS to be applied on newly revised INCOTERMS3000 and educating business circles to use proper conditions of INCOTERMS for their doing business. Secondly, we shall participate in revising INCOTERMS to activate multimodal transport conditions of INCOTERMS and provide solutions to fill gap between a theory and an actual condition of international trade. It seems that terms of multimodal transport such as FCA, CPT, or CIP can be a perfect condition for each party of contract. We have examined the inconsistent features of Ship's rail and notions of on board, and observed how to activate multimodal transport terms. These would be hot issues of next revision of INCOTERMS and we provided improvements on each trade terms, THC charges, or others against INCOTERMS.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제40권6호
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• pp.291-296
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• 2014

Objectives: Bisphosphonate-related osteonecrosis of the jaw (BRONJ) is a side effect of bisphophonate therapy that has been reported in recent years. Osteoclastic inactivity by bisphosphonate is the known cause of BRONJ. Bone morphogenetic protein-2 (BMP-2) plays an important role in the development of bone. Recombinant human BMP-2 (rhBMP-2) is potentially useful as an activation factor for bone repair. We hypothesized that rhBMP-2 would enhance the osteoclast-osteoblast interaction related to bone remodeling. Materials and Methods: Human fetal osteoblast cells (hFOB 1.19) were treated with $100{\mu}M$ alendronate, and 100 ng/mL rhBMP-2 was added. Cells were incubated for a further 48 hours, and cell viability was measured using an MTT assay. Expression of the three cytokines from osteoblasts, receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL), osteoprotegerin (OPG), and macrophage colony-stimulating factor (M-CSF), were analyzed by real-time polymerase chain reaction and enzyme-linked immunosorbent assay. Results: Cell viability was decreased to $82.75%{\pm}1.00%$ by alendronate and then increased to $110.43%{\pm}1.35%$ after treatment with rhBMP-2 (P<0.05, respectively). OPG, RANKL, and M-CSF expression were all decreased by alendronate treatment. RANKL and M-CSF expression were increased, but OPG was not significantly affected by rhBMP-2. Conclusion: rhBMP2 does not affect OPG gene expression in hFOB, but it may increase RANKL and M-CSF gene expression.

• 대한약침학회지
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• 제14권3호
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• pp.19-45
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• 2011

Objectives: This study was performed to analyse the effects of Sweet Bee Venom(Sweet BV-pure melittin, the major component of honey bee venom) on the central nervous system in rats. Methods: All experiments were conducted at Biotoxtech Company, a non-clinical studies authorized institution, under the regulations of Good Laboratory Practice (GLP). Male rats of 5 weeks old were chosen for this study and after confirming condition of rats was stable, Sweet BV was administered in thigh muscle of rats. And checked the effects of Sweet BV on the central nervous system using the functional observational battery (FOB), which is a neuro-toxicity screening assay composed of 30 descriptive, scalar, binary, and continuous endpoints. And home cage observations, home cage removal and handling, open field activity, sensorimotor reflex test/physiological measurements were conducted. Results: 1. In the home cage observation, there was not observed any abnormal signs in rats. 2. In the observation of open field activity, the reduction of number of unit areas crossed and rearing count was observed caused by Sweet BV treatment. 3. In the observation of handling reactivity, there was not observed any abnormal signs in rats. 4. In the observation of sensorimotor reflex tests/physiological measurements, there was not observed any neurotoxic signs in rats. 5. In the measurement of rectal temperature, treatment of Sweet BV did not showed great influences in the body temperature of rats. Conclusions: Above findings suggest that Sweet BV is relatively safe treatment in the central nervous system. But in the using of over dose, Sweet BV may the cause of local pain and disturbance of movement. Further studies on the subject should be conducted to yield more concrete evidences.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제38권
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• pp.48.1-48.8
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• 2016

Background: This study investigates the effect of alendronate-treated osteoblasts, as well as the effect of low-level laser therapy (LLLT) on the alendronate-treated osteoblasts. Bisphosphonate decreases the osteoblastic activity. Various treatment modalities are used to enhance the bisphosphonate-treated osteoblasts; however, there were no cell culture studies conducted using a low-level laser. Methods: Human fetal osteoblastic (hFOB 1.19) cells were treated with $50{\mu}M$ alendronate. Then, they were irradiated with a $1.2J/cm^2$ low-level Ga-Al-As laser (${\lambda}=808{\pm}3nm$, 80 mW, and 80 mA; spot size, $1 cm^2$; NDLux, Seoul, Korea). The cell survivability was measured with the MTT assay. The three cytokines of osteoblasts, receptor activator of nuclear factor ${\kappa}B$ ligand (RANKL), osteoprotegerin (OPG), and macrophage colony-stimulating factor (M-CSF) were analyzed. Results: In the cells treated with alendronate at concentrations of $50{\mu}M$ and higher, cell survivability significantly decreased after 48 h (p < 0.05). After the applications of low-level laser on alendronate-treated cells, cell survivability significantly increased at 72 h (p < 0.05). The expressions of OPG, RANKL, and M-CSF have decreased via the alendronate. The RANKL and M-CSF expressions have increased, but the OPG was not significantly affected by the LLLT. Conclusions: The LLLT does not affect the OPG expression in the hFOB cell line, but it may increase the RANKL and M-CSF expressions, thereby resulting in positive effects on osteoclastogenesis and bone remodeling.

• 한국항만경제학회지
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• 제29권1호
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• pp.123-141
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• 2013

Incoterms, 2010의 FCA, CPT, CIP 등 복합운송물류조건의 이용 활성화 요인 중 대분류 세 가지 요인에 대한 분석결과는 "주관적인 요인"이 3.87점, "무역운송물류 환경적인 요인"이 3.60점, 그리고 "자체 규정 내용적인 요인"이 3.74점이었다. 세 가지 요인의 평균평점이 크게 차이가 않나 세 가지 요인의 복합적인 요인 결합에 의해 복합운송물류조건의 이용 활성화를 도모하여야 한다는 결론이다. 하지만 주관적인 요인의 평균평점이 가장 높다는 것은, Incoterms, 2010의 FCA, CPT, CIP 등 복합운송물류조건의 이용 활성화를 위해서는 이들 조건의 이용자인 기업 스스로가 가장 우선적으로 (1) 복합일관운송과 Incoterms의 각 조건의 연관성을 잘 이해하고, (2) 복합일관운송에는 FCA, CPT, CIP 등의 조건을 가능한 이용하도록 하여야 한다는 것을 의미한다. 그리고 명심할 것은 관행적으로 FOB, CFR, CIF 조건 등을 이용하는 것을 억제하여야 한다는 점이다. 결국 Incoterms, 2010의 FCA, CPT, CIP 등 복합운송물류조건이 복합일관운송에 가장 적합한 조건임을 이해하고 이를 적극적으로 이용하도록 권장하여야 한다는 것이며, 따라서 이를 위한 많은 교육과 홍보가 수반되어야 할 것이다. 그 다음 순위는 무역운송물류 환경적인 요인 (3.60점) 보다 자체 규정 내용적인 요인(3.74)이 더 높은 점수를 기록하고 있는데, 이것은 무역운송물류 환경적인 요소, 즉 국제복합일관운송의 보편화 시대를 맞이하여 국제복합일관운송을 잘 이해하는 것 보다는, FCA, CPT, CIP조건 자체 내용을 잘 이해하고, FCA, CPT, CIP조건이 자사의 무역운송물류환경에 잘 부합한다는 점과 FCA, CPT, CIP조건을 이용하는 것이 자사에게 유리함을 잘 이해하는 것이 보다 중요하다는 것을 의미한다.