• IMMUNE NETWORK
• /
• 제4권4호
• /
• pp.237-243
• /
• 2004

Background: The normal functions of the cell cycle inhibitor p16INK4a are frequently inactivated in many human cancers. Over 80% of hepatocellular carcinoma (HCC) cases lack a functional p16/Rb pathway. p16/Rb pathway, as well as p53 pathway, is considered as one of key components of tumor suppression. Methods: To study the roles of p16INK4a in HCC, a stable cell line expressing exogenous p16 was generated from SNU-449 hepatocellular carcinoma cells lacking endogenous p16, and suppression subtractive hybridization (SSH) was performed in parallel with the control cells. Results: 1) SSH identifies fibronectin (FN1), crystallin ${\alpha}B$ (CRYAB), Rac1, WASP, RhoGEF, and CCT3 as differentially-expressed genes. 2) Among the selected genes, the up-regulation of FN1 and CRYAB was confirmed by Northern blot, RT-PCR and by proteomic methods. Conclusion: These genes are likely to be associated with the induction of stress fiber and stabilization of cytoskeleton. Further studies are required to clarify the possible role of p16 in the signal transduction pathway.

• Transactions on Electrical and Electronic Materials
• /
• 제9권1호
• /
• pp.6-11
• /
• 2008

In this study, we investigated effects of hydrogen annealing (HA) and plasma nitridation (PN) applied in order to improve $Si/SiO_2$ interface characteristics of TiN metal gate. In result, HA and PN showed a positive effect decreasing number of interface state $(N_{it})$ respectively. After FN stress for verifying reliability, however, we identified rapid increase of $N_{it}$ for TiN gate with HA, which is attributed to hydrogen related to a change of $Si/SiO_2$ interface characteristic. In contrast to HA, PN showed an improved Nit and gate oxide leakage characteristic due to several possible effects, such as blocking of Chlorine (Cl) diffusion and prevention of thermal reaction between TiN and $SiO_2$.

• 한국토양비료학회지
• /
• 제9권3호
• /
• pp.165-181
• /
• 1976

서기(西紀) 1980년 '90년(年) 및 2000년대(年代)의 작물생산(作物生産)에 필요(必要)한 삼요소필요량(三要素必要量)을 비료이용율모형(肥料利用率模型)(Fn=Y/E(1-Cs)/Eu) (여기서 Fn; 비료필요량(肥料必要量), Y; 작물생산량(作物生産量), E; 양분효율(養分效率), Cs; 천연양분기여율(天然養分寄與率), Eu; 비료이용율(肥料利用率))과 시비효율(施肥效率) 모형(模型)(Fn=Y(1-Cys)/Fe) (여기서, Cys; 토양생산량기여율(土壤生産量寄與率), Fe; 시비효율(施肥效率))에 의(依)하여 설정(設定)된 단위수량(單位收量)에 필요(必要)한 양분요구량(養分要求量) $N^0$ (Y/E 양분교율(養分郊率)의 역수(逆數))를 사용(使用)하여 예측(豫測)하고 비료필요량(肥料必要量) 및 양분보전(養分保全)의 한계척도(限系尺度)로서의 양분흡수량(養分吸收量)과 작물생산계(作物生産系)에 반환(反還)되지 아니하는 부환삼요소량(不還三要素量)을 산출(算出)하여 비교검토(比較檢討)하였다. 삼요소(三要素) 흡수(吸收) 총량(總量)은 1965년(年) 60만(萬) M/T 1974년(年)에 70만(萬) M/T이었고 1980년(年) 88만(萬) M/T 1990년(年)에 117만(萬) M/T) 2000년(年)에 141만 M/T으로 추정(推定)되며 삼요소(三要素) 필요량(必要量)은 이용률모형(利用率模型)에서 흡수량(吸收量)의 약(約) 90% 여비효율모형(旅肥效率模型)에시 약(約) 87%가 되었으며 부환량(不還量)은 29% 미만(未滿)이었다. 삼요소(三要素) 각필요량(各必要量)은 질소(窒素)는 흡수량(吸收量)과 거의 같고 인산(燐酸)은 흡수량(吸收量)의 1.5배(培) 칼리는 흡수량(吸收量)의 약(約) 반(半)이었다. 비료필요량(肥料必要量) 예측모형(豫測模型)에 주는 품종개량(品種改良) 토양개량(土壤改良) 및 비옥도증진(肥沃度增進) 경종방법개선(耕種方法改善) 비료형태변화(肥料形態變化)의 영향(影響)은 효율치(效率値)의 증대(增大)를 가져와서 비료필요량(肥料必要量)을 감소(減少)시킬 것이나 저온(低溫)과 같은 기상(氣象) 장애(障碍)는 효율치(效率値)를 감소(減少)시켜 비료수요량(肥料需要量)의 증대(增大)를 가져올 것으로 보였다. 1974년(年)의 비료사용(肥料使用) 현황(現況)은 특(特)히 질소(窒素)와 인산(燐酸)에 상당히 낭비적(浪費的)인 것으로 나타났으며 그럼에도 비옥도(肥沃度)가 향상(向上)되지 않은 곳이 많은 것을 볼 때 비료시용(肥料施用)의 과학화(科學化)가 시급(時急)한 것으로 나타났다.

• 한국작물학회지
• /
• 제42권1호
• /
• pp.89-94
• /
• 1997

토양수분 부족이 콩의 생육 및 수양에 미치는 영향을 알아보고자 비가림 비닐하우스 안의 베드에서 우리나라 기상여건상 토양수분 부족의 유발가능성이 높은 영양생장기인 신장기에 제 3 복엽기부터 30일간 단수(FNS)와 생육중기인 착협시부터 30일간 단수(BPS), 생육후기 인 입비대시부터 30일간(BSS) 토양수분 부족을 유발시켜 시험을 수행한 결과를 요약하면 다음과 같다. 1. 지상부의 건물중은 FNS 단수처리시 감소율이 가장 컸으며, BPS와 BSS 단수처리시는 대조구와 큰 차이가 없었다. 2. 엽록소 함량은 FNS와 BPS 단수처리시 처리종료 직후에는 현저히 감소되었으나, 수확기에는 회복이 되어 대조구와 유사하였다. 3. 한발처리 종료직후의 토양깊이별 뿌리 분포는 단수처리시 대조구에 비해 지표면 가까이에서 분포되 었으며, 뿌리혹 수와 뿌리혹의 건물중은 BPS 단수처리시 감소가 가장 심하였다. 4. 개체당 협수, 개체당 입중 및 수양은 단수처리시 대조구에 비해 현저하게 감소되었으며, BPS 단수처리시 감소율이 가장 심하였다.

• 한국정보통신학회논문지
• /
• 제16권5호
• /
• pp.999-1008
• /
• 2012

ISB(Intelligent Silicon Bead)는 기존 CMOS 칩과 달리 CMOS SoC의 전원을 포함한 외부와의 인터페이스(interface)를 모두 빛을 이용하여 칩의 초소형화 및 단가를 낮추고 광통신, 메모리 기능을 탑재한 신개념 바이오 실험용 칩이다. 본 논문에서는 외부리더기로부터 비드칩에 인가된 하나의 광신호를 통해 전력과 신호를 동시에 전달하기 위한 저전력, 저면적특성의 광수신단 설계와 입력프로토콜에 대해서 소개하고 이를 시뮬레이션 및 측정을 통해 검증한다. 또한 칩의 ID를 기록/저장하기 위한 저전력 PROM을 설계하여 광신호 입력에 따른 출력 결과 값을 얻는데 성공한다. 본 연구를 통하여 기존 RFID에서 발생한 칩면적 소형화의 한계와 높은 단가 등의 문제점을 해결하여 새로운 유형의 바이오용 칩 개발을 기대할 수 있다.

• Plant Biotechnology Reports
• /
• 제4권2호
• /
• pp.165-172
• /
• 2010

Genes that are expressed early in specific response to high salinity conditions were isolated from rapeseed plant (Brassica napus L.) using an mRNA differential display method. Five PCR fragments (DD1.5) were isolated that were induced by, but showed different response kinetics to, 200 mM NaCl. Nucleotide sequence analysis and homology search revealed that the deduced amino sequences of three of the five cDNA fragments showed considerable similarity to those of ${\beta}$-mannosidase (DD1), tomato Pti-6 proteins (DD5), and the tobacco harpin-induced protein hin1 (DD4), respectively. In contrast, the remaining clones, DD3 and DD2, did not correspond to any substantial existing annotation. Using the DD3 fragment as a probe, we isolated a full-length cDNA clone from the cDNA library, which we termed BnSWD1 (Brassica napus salt responsive WD40 1). The predicted amino-acid sequence of BnSWD1 contains eight WD40 repeats and is conserved in all eukaryotes. Notably, the BnSWD1 gene is expressed at high levels under salt-stress conditions. Furthermore, we found that BnSWD1 was upregulated after treatment with abscisic acid, salicylic acid, and methyl jasmonate. Our study suggests that BnSWD1, which is a novel WD40 repeat-containing protein, has a function in salt-stress responses in plants, possibly via abscisic acid-dependent and/or -independent signaling pathways.

• 아태비즈니스연구
• /
• 제14권1호
• /
• pp.149-169
• /
• 2023

Purpose - The main purpose of this study is to develop the stress test model for Korean banks by exploring the optimal Monte Carlo simulation and BIS forecasting model. Design/methodology/approach - This study selects 15 Korean banks as sample financial firms and collects relevant 76 quarterly data for the period between year 2000 and 2018 from KRX(Korea Excange), Bank of Korea, and FnGuide. The Regression analysis, Unit-root test, and Monte Carlo simulation are hired to analyze the data. Findings - First, most of the sample banks failed to keep 8% BIS ratio for the adverse and severely Adverse Scenarios, implying that Korean banks must make every effort to realize better BIS ratios under adverse market conditions. Second, we suggest the better Monte Carlo simulation model for the Korean banks by finding that the more appropriate volatility should be different depending on variables rather than simple two-sigma which has been used in the previous studies. Third, we find that the stepwise regression model is better fitted than simple regression model in forecasting macro-economic variables for the BIS variables. Fourth, we find that, for the more robust and significant statistical results in designing stress tests, Korean banks are required to construct more valid time-series and cross-sectional data-base. Research implications or Originality - The above results all together show that the optimal volatility in designing optimal Monte Carlo simulation varies depending on the country, and many Korean banks fail to pass sress test under the adverse and severely adverse scenarios, implying that Korean banks need to make improvement in the BIS ratio.

• 한국통신학회논문지
• /
• 제16권11호
• /
• pp.1179-1185
• /
• 1991

초박막 재산화 질화산화막을 $1050^{\circ}C-1100^{\circ}C$ 온도에서 20, 40초 동안 산소 분위기에서 램프 가열 방법의 급속 열처리 공정에 의해 형성 시켰다. 초박막의 전기적 특성은 누설전류, 항복전압, 시간종속 항복과 F-N 관통을 분석 하였다. 질화와 재산화 조건에 따른 전하포획의 의존성 즉 고전계 스트레스에 유기되는 항복전하량$(Q_{BD})$ 증가 여부와 평탄대역 전압이동$(\DeltaV_{FB})$을 연구하였다. 분석 결과에 의하면, 급속 열처리 재산화시 유전적 성질이 상당히 개선되었고, 항복전하량은 증가되었으며, 평탄대역전압은 감소 되었다.

• 한국전기전자재료학회:학술대회논문집
• /
• 한국전기전자재료학회 2008년도 추계학술대회 논문집 Vol.21
• /
• pp.122-122
• /
• 2008

Recently, Metal/Alumina/Silicon-Nitride/Silicon-Oxide/Silicon (MANOS) structures are one of the most attractive candidates to realize vertical scaling of high-density NAND flash memory [1]. However, as ANO layers are miniaturized, negative and positive bias temperature instability (NBTI/PBTI), such as the flat band voltage shift, ${\Delta}V_{FB}$, the interfacial trap density increase, ${\Delta}D_{it}$, the gate leakage current, ${\Delta}I_G$. and the retention characteristics, in MONOS capacitors, becomes an important issue in terms of reliability. It is well known that tunnel oxide degradation is a result of the oxide and interfacial traps generation during FN (Fowler-Nordheim) stress [2]. Because the bias temperature stress causes an increase of both interfacial-traps and fixed oxide charge could be a factor, witch can degrade device reliability during the program and erase operation. However, few studies on NBTI/PBTI have been conducted on improving the reliability of MONOS devices. In this work, we investigate the effect of post-annealing gas on bias temperature instability (BTI), such as the flat band voltage shift, ${\Delta}V_{FB}$, the interfacial trap density shift, ${\Delta}I_G$ retention characteristics, and the gate leakage current characteristics of MANOS capacitors. MANOS samples annealed at $950^{\circ}C$ for 30 s by a rapid thermal process were treated via additional annealing in a furnace, using annealing gases $N_2$ and $N_2-H_2$ (2 % hydrogen and 98 % nitrogen mixture gases) at $450^{\circ}C$ for 30 min. MANOS samples annealed in $N_2-H_2$ ambient had the lowest flat band voltage shift, ${\Delta}V_{FB}$ = 1.09/0.63 V at the program/erase state, and the good retention characteristics, 123/84 mV/decade at the program/erase state more than the sample annealed at $N_2$ ambient.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제25권4호
• /
• pp.337-349
• /
• 1999

The extracellular matrix(ECM) is a complex network of different combination of collagens, glycosaminoglycans, laminin, fibronectin, and many other glycoproteins including proteolytic enzymes. The composition and organization of the ECM contributes to the uniques physical or biomechanical properties of a tissue. Fibronectins(FN) are dimeric glycoproteins located on cell surfaces, in the matrix of connective tissue, and in blood. Fibronectins mediate cell attachment to collagen substratum and have been implicated in a variety of important biological processes, including embryogenesis and cell differentiation. The purpose of this study was to determine the effects of surgical induction of anterior disk displacement(ADD) on distribution of fibronectin in the rabbit temporomandibular joint(TMJ) tissues included the articular cartilage, disc, retrodiscal tissue, articular eminence using an immunohistochemical technique. The left TMJ was exposed surgically, and all discal attachments were severed except for the posterior attachment. The disk was then repositioned anteriorly and sutured to the zygomatic arch. The right TMJ served as a shamoperated control. Normal joints were used as a nonoperated control. Fourty-five rabbits were used for experiments in total. For fibronectin immunohistochemical study, eighteen rabbits (one normal group and 5 experimental groups, each group consists of 3 rabbits) were used. The experimental rabbits were sacrified after operation period of 2, 3, 4, 6 and 8 weeks on fibronectin. The obtained results were as follows ; 1. Fibronectin immunoreaction on all TMJ tissues(mandibular condyle, articular disc, retrodiscal tissue, articular eminence) in the normal rabbit was observed. Especially the reverse cell layer and proliferation zone of articular cartilage of condyle show strong positive reaction. 2. Depletion of fibronectin in the all TMJ tissues except hypertrophic zone of articular cartilage occurred at 2 weeks following induction of ADD. 3. The restoration of immunoreaction at 4 weeks was observed and a progressive increasing reaction at 6 weeks, 8 weeks also was found. Our study generally showed degenerative changes in TMJ tissues after ADD although TMJ tissues adapted or degenerated to abnormal loads and stress distribution according to the remodeling capacity of TMJ tissues.