• Korean Journal of Poultry Science
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• v.48 no.2
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• pp.81-90
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• 2021

Avian influenza viruses (AIVs) must utilize host cellular factors to complete their life cycle, and fragile X mental retardation protein (FMRP) has been reported to be a host factor promoting AIV ribonucleoprotein (vRNP) assembly and exports vRNP from the nucleus to the cytoplasm. The functional role of chicken FMRP translational regulator 1 (cFMR1) as a host factor of AIV is, however, poorly understood. In this study, we targeted the cFMR1 gene in DF1 cells using clustered regularly interspaced short palindromic repeats/Cas9-mediated genome editing to examine the functional role of cFMR1 as a host factor of AIV. We found that cFMR1 stimulated viral gene transcription during early stages of the viruses' life cycle and did not affect viral progeny production and viral polymerase activity in DF1 cells 24 hours post infection. cFMR1 overexpression did not exert significant effects on virus production, compared to the control. Therefore, unlike in mammalian systems (e.g., humans or mice), cFMR1 did not play a pivotal role in AIV but only seemed to stimulate viral proliferation during early stages of the viral life cycle. These results imply that the interplay between host factors and AIV differs between mammals and avian species, and such differences should be considered when developing anti-viral drugs for birds or establishing AIV-resistant bird models.

• Journal of the Korean Magnetics Society
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• v.6 no.5
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• pp.298-304
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• 1996

We have manufactured FMR spectrometer over wide range(2-35 GHz). In order to test FMR spectrometer, res¬onance absorption has been measured of the standard sample DPPH. The Q vaules of absorption line are 189-1096. As a result, We noticed that FMR spectrometer has been manufactured well. FMR studies of MgFez04 single crystal have been performed at S, J, K-band. The resonance lines have been observed for the each orientation of (100) plane at 300 K. The values of the magnetic anisotropy constant $K_{1}$ and the spectroscopic spli tting g valule have been calculated from the ferromagnetic resonance curve, $-2.9{\times}10^{4}erg/cm^{3}$, 2.02 at 23.89 GHz, $-2.2{\times}10^{4}erg/cm^{3}$, 1.89 at 5.3 GHz and $-2.8{\times}10^{4}erg/cm^{3}$, 2.01 at 3.6 GHz.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• v.11 no.1
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• pp.3-15
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• 2000

Objectives：There has been a rapid expansion of studies aimed at elucidating the genetic basis of autistic disorder, especially it’ relationship to fragile-X syndrome. The detection of fragile X chromosome(Xq27.3) by cytogenetic analysis has revealed many difficulties in testing. Therefore, to explore the relationship between autistic disorder and fragile X syndrome, this study administered molecular biologic methods which examined an unstable CGG repeat within the fragile X mental retardation-1(FMR-1) gene. Methods：Ninety nine autistic children and eight normal control children were tested. The number of CGG repeats within FMR-1 gene was measured after amplification by PCR, and cytogenetic analysis was also carried out to detect fragile site Xq27.3. Southern blot hybridization, using StB12.3 and/or Pfxa3 probe, was done for the patients showing expansion of more than 50 CGG repeats (premutation). Results：All but two autistic patients had no expansion in CGG repeats by PCR and there was no significant statistical difference in number of CGG repeat in comparison with normal control. Two autistic patients, considered as premutation by PCR analysis, had no full mutation or premutation by Southern blot hybridization. All autistic children tested did not have any abnormal karyotype or fragile site Xq27.3. Conclusions：These results suggest that autistic patients may not have abnormality in FMR-1 gene or abnormal expansion in CGG repeat. In conclusion, fragile X syndrome may not be antecedent of autistic disorder.

• Journal of Genetic Medicine
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• v.5 no.1
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• pp.41-46
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• 2008

Purpose : Fragile X syndrome (FXS) is the most common heritable cause of cognitive impairment. FXS is caused by hyperexpansion and hypermethylation of a polymorphic CGG trinucleotide repeat in the 5' untranslated region of the fragile X mental retadation-1(FMR1) gene. Combination of Southern blotting and simple polymerase chain reaction(PCR) amplification of the FMR1 repeat region is commonly used for diagnosis in females. To give a definite diagnosis in a female child suspected of having FXS, we carried out the molecular diagnostic test for FXS using the recently developed Abbott Molecular Fragile X PCR Kit. Methods : The PCR amplification of the FMR1 repeat region was performed using the Abbott Mdecular Fragile X PCR Kit. The amplified products were analyzed by size-separate analysis on 1.5% agarose gels and by DNA fragment analysis using Gene scan. Results : Agarose gel and Gene scan analyses of PCR products of the FMR1 repeat region showed that the patient had two heterozygous alleles with a normal 30 repeats and full mutation of >200 repeats whereas her mother had two heterozygous alleles with the normal 30 repeats and premutation of 108 repeats, suggesting that the premutation of 108 repeats in her mother may have led to the full mutation of >200 repeats in the patient. Conclusion : We diagnosed FXS in a female patient using a simplified molecular diagnostic test. This commercially available diagnostic test for FXS, based on PCR, may be a suitable alternative or complement method to Southern blot analysis and PCR analysis and/or methylation specific(MS)-PCR analysis for the molecular diagnosis of FXS in both males and females.

• The Journal of Korean Society for School & Community Health Education
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• v.11 no.1
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• pp.27-39
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• 2010

Objectives: This study was conducted to find out caries preventive effect on permanent teeth among children who brush teeth with fluoride-containing toothpaste by supervised weekly fluoride mouthrinsing program in elementary schools. Methods: The epidemiologic dental survey was performed on the elementary schoolchildren of supervised weekly fluoride mouthrinsing program (FMR) with 0.2 percent neutral sodium fluoride solution and control group in 2007. Caries preventive effect on permanent teeth by fluoride mouthrinsing program were calculated by DMFT index and DMFS index between FMR group and control group. Results: By DMFT index between FMR group and control group, caries rates on permanent teeth of the fourth-, fifth- and sixth-grade children participating in FMR program were 34.1%, 40.8% and 31.5%, respectively. By the DMFS index between FMR group and control group, caries preventive rates on permanent teeth of the fourth-, fifth- and sixth-grade children participating in FMR program were calculated 25.4%, 37.7% and 33.5%, respectively. Conclusions: We suggest that fluoride mouthrinsing program should be developed to all elementary schools to prevent dental caries.

• Journal of the Korean Ceramic Society
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• v.32 no.2
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• pp.163-170
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• 1995

Sample size effect on ferromagnetic resonance (FMR) in polycrystalline MgFe2O4 has been investigated. The signal intensity (SI), resonance field (Hres) and line width (ΔH) were found to increase proportionally to r3 with the increase of sample radius. The r3-depencence of SI means the complete penetration of rf-field into the sample, and the broadening of ΔH due to the sample size appears to be closely related to the amount of scattering sources like pores. Meanwhile, the values of Hres (0) and ΔH (0) obtained by extrapolating the data of Hres (r) and ΔH (r) measured at several sizes to r=0, were in good agreement with those calculated using the Schlomann's equations for internal field and ΔH, respectively. This result indicates that the discrepancy between the measured FMR parameters and those calculated by Schlomann's equation could be ascribed to the effect of sample size. Thus it is suggested that the size effect on FMR should be removed for the analysis of the FMR parameters. Meanwhile, our result for the size dependance of ΔH was found to be contradictory to those reported by Dionne, where ΔH 1/r at a given surface roughness. This discrepancy appears to arise from the difference in the definition of reading the line width.

• Molecules and Cells
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• v.23 no.2
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• pp.228-238
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• 2007

Since molecular structure of hnRNP is not available in foreseeable future, it is best to construct a working model for hnRNP structure. A geometric problem, assembly of $700{\pm}20$ nucleotides with 48 proteins, is visualized by a frame work in which all the proteins participate in primary binding, followed by secondary, tertiary and quaternary binding with neighboring proteins without additional import. Thus, 40S hnRNP contains crown-like secondary structure (48 stemloops) and appearance of 6 petal (octamers) rose-like architectures. The proteins are wrapped by RNA. Co-transcriptional folding for RNP fibril of FMR1 gene can produce 2,571 stem-loops with frequency of 1 stem-loop/15.3 nucleotides and 53 40S hnRNP beaded structure. By spliceosome driven reactions, there occurs removal of 16 separate lariated RNPs, joining 17 separate beaded exonic structures and anchoring EJC on each exon junction. Skipping exon 12 has 5'GU, 3'AG and very compact folding pattern with frequency of 1 stem-loop per 12 nucleotides in short exon length (63 nucleotides). 5' end of exon 12 contains SS (Splicing Silencer) element of UAGGU. In exons 10, 15 and 17 where both regular and alternative splice sites exist, SS (hnRNP A1 binding site) is observed at the regular splicing site. End products are mature FMR-1 mRNP, 4 species of Pri-microRNAs derived from introns 7,9,15 and 3'UTR of exon17, respectively. There may also be some other regulatory RNAs containing ALU/Line elements as well.

• Journal of the Korean Magnetics Society
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• v.2 no.2
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• pp.105-113
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• 1992

조성이 Mn$_{x}$Zn$_{1-x}$ Fe$_{2}$O$_{4}$ 훼라이트(x=0.75, 0.52)를 pusher type 연속 전기로를 이용하여 질소 분위기하에서 1360 .deg. C 로 3시간 동안 소결하여 얻어냈다. 본 연구에서는 스피넬 구조인 Mn-Zn 훼라이트의 FMR(Ferrimagnetic Resonance) 스펙트럼 특징과 초기투자율과의 상관성을 조사하고자 EPR X-band 스펙 트로미터를 이용 9.50 GHz의 microwave frequency에서 FMR 실험을 수행하였다. FMR 공명흡수선은 주 자기공명 흡 수선외에 낮은 자기장에서 약한 subsidiary 공명흡수선을 나타내는데 온도변화에 따른 주 자기공명 흡수선과 sub- sidiary 공명흡수선(제2, 제3 공명흡수선)의 세기, 공명선폭, g값 등의 온도 의존성을 연구함으로써 제2 공명흡수 선이 나타나는 원인을 분석하였으며, 자기공명 흡수선의 g값, 자기공명선 세기, 그리고 공명선폭과 초기투자율의 상관성을 연구하였다. 실험 결과 주 자기공명 흡수선의 g값, 자기공명선 세기, 공명선폭은 초기투자율 값과 밀접 한 관련이 있음을 확인할 수 있었다.었다.

• Journal of Magnetics
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• v.4 no.3
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• pp.92-97
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• 1999

A relationship between microstructure and ferromagnetic resonance of Co/Ag multilayers has been studied in as-deposited and annealed multilayers iwth ultrathin (dCo < 1 nm) Co layers. Depending on the nominal thickness of Co Co/Ag multilayers represent a system of fine magnetic particles (dCo < 0.4 nm) or discontionuous layered structure (dCo/0.5 nm). FMR data has been interpreted in the framework of a odel of interacting fine particles exhibiting superparamagnetic behavior. Changes in the FMR spectra upon annealing have been attributed to the growth of the Co particles and to a transition from the fcc to hcp atomic structure of the highly (111) textured Co particles.

• Journal of the Korean Magnetics Society
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• v.24 no.1
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• pp.11-17
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• 2014

We measured the ferromagnetic resonance (FMR) signal using the monodisperse iron oxide nanoparticles with size D=4.67 nm, 5.64 nm and 6.34 nm synthesized by using the thermal decomposition method, respectively. The measured ferromagnetic resonance signals were compared with the calculated ones for superparamagnetic nanoparticles with lognormal volume distribution. The FMR linewidth broadening was propositional to tanh($V^2$), where V was volume of nanoparticles. The narrow linewidth of small size nanoparticles was due to the surface spins, while the broad linewidth of large size nanoparticles was due to the bulk spins affected by the crystalline structure of iron oxide nanoparticles. The superposition of surface and bulk effect was confirmed at D=5.64 nm nanoparticles, which was near the critical size for linewidth transition from surface effect to bulk effect.