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The Effects of Salt Stress on Photosynthetic Electron Transport and Thylakoid Membrane Proteins in the Cyanobacterium Spirulina platensis

  • Sudhir, Putty-Reddy;Pogoryelov, Denys;Kovacs, Laszlo;Garab, Gyozo;Murthy, Sistla D.S.
    • BMB Reports
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    • v.38 no.4
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    • pp.481-485
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    • 2005
  • The response of Spirulina (Arthrospira) platensis to high salt stress was investigated by incubating the cells in light of moderate intensity in the presence of 0.8 M NaCl. NaCl caused a decrease in photosystem II (PSII) mediated oxygen evolution activity and increase in photosystem I (PSI) activity and the amount of P700. Similarly maximal efficiency of PSII (Fv/Fm) and variable fluorescence (Fv/Fo) were also declined in salt-stressed cells. Western blot analysis reveal that the inhibition in PSII activity is due to a 40% loss of a thylakoid membrane protein, known as D1, which is located in PSII reaction center. NaCl treatment of cells also resulted in the alterations of other thylakoid membrane proteins: most prominently, a dramatic diminishment of the 47-kDa chlorophyll protein (CP) and 94-kDa protein, and accumulation of a 17-kDa protein band were observed in SDS-PAGE. The changes in 47-kDa and 94-kDa proteins lead to the decreased energy transfer from light harvesting antenna to PSII, which was accompanied by alterations in the chlorophyll fluorescence emission spectra of whole cells and isolated thylakoids. Therefore we conclude that salt stress has various effects on photosynthetic electron transport activities due to the marked alterations in the composition of thylakoid membrane proteins.

Survey on Rate of Twin Pregnancy and Parturition in Dairy Cows in Korea (국내 유우(Holstein)의 쌍태임신율 및 분만에 관한 조사)

  • Cho, Jin-Haeng;Kim, Myung-Cheol;Jeong, Seong-Mok;Lee, Jae-Yeon;Shin, Sang-Tae;Shin, Beom-Jun
    • Journal of Veterinary Clinics
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    • v.29 no.1
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    • pp.33-37
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    • 2012
  • This study was performed to evaluate the rate of twin pregnancy and parturition in dairy cows. Calving records of Holstein dairy cows from 1998 to 2009 comprising Goyang and Paju cities herd with 20,990 calving events representing 820 twin births were used to evaluate twinning rate, calf sex ratio, periods of pregnancy and complication after parturition in single and twin births. Overall, the reported twinning rate was 3.9% in Holstein dairy cows. Rate of bicornual pregnancy (75%) was larger than that of unicornual pregnancy (25%) among cows having twin. Regardless of parity, the greatest twinning rate was observed in fall season from September to November. Calf sex ratios (male, M; female, F) were 48.0% FM, 28.9% MM, 21.5% FF and 0.9% mummifications for twin calves. Parturition type among cows having twins included normal (4.7%), premature (66.9%), delayed (9.2%), abortion (18.4%) and mummification (0.8%). The period of pregnancy in twin pregnancy (mean 272.6 days) was shorter than single pregnancy (mean 278.1 days). The retained placenta after parturition was over fourth times such as twin parturition (34.8%) higher than single parturition (8.5%) and the abomasal displacement was over two times such as twin parturition (10.2%) higher than single parturition (4.9%). The distribution of twin pregnancy with parities was high rate during the 2~3 parities. The prevalence of complication such as retained placenta and abomasal displacement with twin parturition revealed higher than single parturition.

Development of Computer Vision System for Individual Recognition and Feature Information of Cow (II) - Analysis of body parameters using stereo image - (젖소의 개체인식 및 형상 정보화를 위한 컴퓨터 시각 시스템 개발(II) - 스테레오 영상을 이용한 체위 분석 -)

  • 이종환
    • Journal of Biosystems Engineering
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    • v.28 no.1
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    • pp.65-76
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    • 2003
  • The analysis of cow body parameters is important to provide some useful information fur cow management and cow evaluation. Present methods give many stresses to cows because they are invasive and constrain cow postures during measurement of body parameters. This study was conducted to develop the stereo vision system fur non-invasive analysis of cow body features. Body feature parameters of 16 heads at two farms(A, B) were measured using scales and nineteen stereo images of them with walking postures were captured under outdoor illumination. In this study, the camera calibration and inverse perspective transformation technique was established fer the stereo vision system. Two calibration results were presented for farm A and fm B, respectively because setup distances from camera to cow were 510 cm at farm A and 630cm at farm B. Calibration error values fer the stereo vision system were within 2 cm for farm A and less than 4.9 cm for farm B. Eleven feature points of cow body were extracted on stereo images interactively and five assistant points were determined by computer program. 3D world coordinates for these 15 points were calculated by computer program and also used for calculation of cow body parameters such as withers height. pelvic arch height. body length. slope body length. chest depth and chest width. Measured errors for body parameters were less than 10% for most cows. For a few cow. measured errors for slope body length and chest width were more than 10% due to searching errors fer their feature points at inside-body positions. Equation for chest girth estimated by chest depth and chest width was presented. Maximum of estimated error fur chest girth was within 10% of real values and mean value of estimated error was 8.2cm. The analysis of cow body parameters using stereo vision system were successful although body shape on the binocular stereo image was distorted due to cow movements.

MIRIS 지구관측 적외선카메라 인증모델 성능 시험 및 Field Test

  • Mun, Bong-Gon;Park, Yeong-Sik;Lee, Chang-Hui;Park, Seong-Jun;Cha, Sang-Mok;Lee, Dae-Hui;Jeong, Ung-Seop;Nam, Uk-Won;Park, Jang-Hyeon;Yuk, In-Su;Ga, Neung-Hyeon;Lee, Mi-Hyeon;Lee, Deok-Haeng;Yang, Sun-Cheol;Kim, Yeong-Ju;Lee, Gi-Hun;Jeong, Han;Lee, Seung-U;Han, Won-Yong
    • Bulletin of the Korean Space Science Society
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    • 2009.10a
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    • pp.45.1-45.1
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    • 2009
  • 과학기술위성 3호의 주탑재체인 MIRIS (Multi-purpose InfraRed Imaging System)는 우주관측카메라 (Space Observation Camera, SOC)와 지구관측카메라 (Earth Observation Camera, EOC)가 독립적인 시스템으로 구성되어 있다. 지구관측카메라는 유효 구경 100 mm, F/5의 광학계로 3-5 마이크론 파장영역을 관측하며, 국내에서 개발된 적외선 검출기의 우주 인증 시험과 유사시 한반도 적외선 감시를 주요 목적으로 하고 있다. 고도 700km에서 지상을 볼 때 약 42m/pixel의 공간분해능을 나타낼 것으로 기대하고 있다. 지구관측카메라의 인증 모델(Qualification Model)은 냉동기를 제외한 모든 부품이 국내기술로 제작되었으며, 미러 본딩 및 릴레이 렌즈 조립 기술, 적외선 영상 검교정 기술 등 다양한 경험과 도전을 제공했다. 이 발표에서는 지구관측카메라 인증모델을 이용하여 수행한 주요 시험 과정을 소개한다. 국내 회사 (주)i3 system에서 제작된 적외선 검출기는 $320\times256$ HgCdTe array (평균 양자효율 80% 이상) 이며 77K에서 정상적으로 운영된다. Micro Stirling Cooler에 의해 듀어는 전원을 켠 후 5분 이내에 검출기 운영온도인 77K까지 내려간다. 적외선 광학계의 정렬, 시스템 MTF 측정, 흑체 측정 및 검교정 작업을 수행한 후 야외에서 다양한 경우에 대해 Field Test를 진행했다. 이 발표에서는 Field Test 과정과 이를 통해 얻은 결과를 발표하고, FM (Flight Model) 제작에 있어 수정해야 할 사항들을 제안해 본다.

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The Study of Alluvial Fans in Korea (한국(韓國) 선상지(扇狀地) 연구(硏究))

  • Park, No-Sik
    • Journal of the Speleological Society of Korea
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    • no.68
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    • pp.5-22
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    • 2005
  • Since earlier in 1947 the writer had been devoting part of his time to study alluvial fans in Korea based on his own interest in geomorphology. However, it was in 1958 when a new geography department was set up and the Research Center of National Lands was established in the university of Kyung Hee, the writer started scientific investigation for alluvial fans. The Following alluvial fans have been studied by the writer: those of Mt. Kwanak and Mt. Chungge in 1947; those of Pulkuksa in 1948; those of Suhnamsa, Suh Myun, Pusan in 1950; those of Shindonae, Mt. Kerrong, in summer of 1958; those of Hwaomsa, Kure and Yongsan Myun, Changhung, in winter of 1958. The article is the comparative study of the above mentioned alluvial fans including Suhkwangsa Fan, Chugaryng Rift Valley. Of course this is not the study covering all af alluvial fans in Korea. Within the limits of his surbey, the writer has com to a conclusion which may be helpful for the Korean alluvial fm study. 1. Korean alluvial fans are generally found in rift valleys or basins. 2. They are formed in places of below 220-meter contour line. 3. The slope from fna apex to river valley is 20-40 degree. The slope of fan surface is 5-20 degree, but most of them under 16 degree. A sudden change of slope is taken place or the fan apex and fan center. 4. Hills (100-200m) are developed on the surfaces of alluvial fans. 5. Most of rivers in alluvial fans are geyser rivers. 6. The depth of well : from ground surface to the well water surface is 4-8 m; water depth is 1-2m. 7. Land utilization; cultivated land 30%: non cultivatedland 70%. The area occupied by upland fields is twice of paddy field area. 8. Settlement : agglomerated settlement is developed on the top or the end of fan and surrounded by windbreakers. 9. Farming pattern at the top of fan fields are rectangular and parallel to flumes but at the center of fan, they are irregular and perpendicular to flumes.

How does the world in the game reveal the end of the century? -Analysis on the method of the representation of the end of the century in the game : With focus on Resident Evil 3, Parasite Eve 2, Silent Hill 3- (게임 속의 세계는 세기말을 어떤 방식으로 드러내는가? -게임 속 세기말의 구현 방식에 관한 분석 : Resident Evil 3, Parasite Eve 2, Silent Hill 3 중심으로-)

  • Ok, Seon Young
    • Journal of Korea Game Society
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    • v.21 no.1
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    • pp.3-24
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    • 2021
  • This paper deals with on the game's way of embodying the society at the end of the century. Resident Evil, Parasite Eve, and Silent Hill are the targets of the analysis. First, I examine the world view of each game and its own language that conveys its contents. Second, I analyze the specific parts of reality that the three games imitate and the way they are represented. Third, I consider the connection between the real world and the world embodied in the game. Fourth, I suggest the possibility of providing an 'alternative perspective' of game media that reveals the hidden aspects of reality in a way of representation. In conclusion, the image of the game becomes 'the eye' that complements our perspective, captures the hidden aspects of reality, obtains a meaningful part, and even presents its blueprint.

Design and Development of 200 W TRM on-board for NEXTSat-2 X-band SAR (차세대소형위성2호의 X대역 합성 개구 레이더 탑재를 위한 200 W급 송·수신 모듈의 설계 및 개발)

  • Jeeheung Kim;Hyuntae Choi;Jungsu Lee;Tae Seong Jang
    • Journal of Advanced Navigation Technology
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    • v.26 no.6
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    • pp.487-495
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    • 2022
  • This paper describes the design and development of a high-power transmit receive module(TRM) for mounting on X-band synthetic aperture radar(SAR) of the NEXTSat-2. The TRM generates a high-power pulse signal with a bandwidth of 100 MHz in the target frequency range of X-band and amplifies a low-noise on the received signal. Tx. path of the TRM has output signal level of more than 200 watts (53.01 dB), pulse droop of 0.35 dB, signal strength change of 0.04 dB during transmission signal output, and phase change of 1.7 ˚. Rx. path has noise figure of 3.99 dB and gain of 37.38 ~ 37.46 dB. It was confirmed the TRM satisfies all requirements. The TRM mounted on the NEXTSat-2 flight model(FM) which will be launched using the KSLV-II (Nuri).

핵융합로용 플라즈마 대향부품 개발을 위해 제작된 텅스텐/FM강 HIP 접합 목업의 수명 평가 해석

  • Lee, Dong-Won;Sin, Gyu-In;Kim, Seok-Gwon;Jin, Hyeong-Gon;Lee, Eo-Hwak;Yun, Jae-Seong;Mun, Se-Yeon;Hong, Bong-Geun
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.452-452
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    • 2014
  • 블랑켓 일차벽이나 디버터와 같은 핵융합로 플라즈마 대향부품은 플라즈마로부터 입사되는 중성자 및 입자들을 차폐하여 구조물을 보호하고, 발생열을 에너지로 변환하기 위해 냉각재를 활용한 열제거 기능을 담당한다. 특히, 고속중성자와 입사 열부하 및 여러 입자들로부터 블랑켓 및 내부 구조물을 보호하기 위해 차폐체와 구조물로 구성된다. 세계적으로 차폐체로서는 텅스텐 혹은 텅스텐 합금, 구조물용 재료로는 저방사화 Ferritic Martensitic (FM) 강이 유력한 후보재료로 개발, 연구 중에 있다. 국내에서는 국제핵융합로(ITER) 사업을 통해 고온등방가압(HIP, Hot Isostatic Pressing)을 이용한 이종금속간 접합기술과 한국형 저방사화 고온구조재료인 ARAA (Advanced Reduced Activation Alloy)가 개발되고 있으며, 이를 활용한 설계, 접합법 개발, 제작목업의 건전성 평가 등이 수행되고 있다. 한국원자력연구원에서는 핵융합 기초사업의 일환으로 전북대와 공동으로 수행 중인 건전성 평가체계 개발을 위해, 기 개발된 접합법을 활용한 $45mm(H){\times}45mm(W){\times}2mm(T)$의 W/FM강 목업을 제작한 바 있으며, 이를 국내 구축된 고열부하 시험 장비인 KoHLT-EB (Electron Beam)를 활용한 고열부하 인가 건전성 평가시험을 준비 중에 있다. 이종금속간 접합 특성은 기계적 평가를 위한 파괴시험을 통해 검증, 이를 활용한 목업이 제작되었으며, 제작된 목업에 대한 초음파를 이용한 접합면의 비파괴 검사를 통해 결함이 없음을 확인하였다. 최종적으로 실제 사용되는 핵융합 운전조건과 유사 혹은 가혹한 조건에서 고열부하를 인가하여, 그 건전성을 평가가 이루어질 것이다. 고열부하 시험을 위해서는 냉각조건, 인가 열부하, 수명평가를 통한 반복 고열부하 인가 횟수 등이 사전에 결정되어야 한다. 이를 위해 상업용 열수력, 구조해석 코드인 ANSYS-CFX와 -mechanical을 이용한 시험조건 모의 및 수명 평가가 수행되었다. 구축 장비의 냉각계통을 고려하여 냉각수의 온도 및 속도는 $25^{\circ}C$, 0.15 kg/sec로, 열부하는 0.5 및 $1.0MW/m^2$에 대해 모의를 수행하였다. 정상상태 시 텅스텐의 최대 온도는 각 열부하 조건에 따라 $285.3^{\circ}C$$546.8^{\circ}C$였으며, 이에 도달하는 시간을 구하기 위해 천이해석을 수행하였고, 이를 통해 30초에 최대온도 95 %이상의 정상상태 온도에 도달함을 확인하였다. 또한, 목업의 초기 온도에 도달하는 냉각시간도 동일한 천이해석을 통해 30초로 가능함을 확인하였고, 최종 시험 조건을 30초 가열, 30초 냉각으로 결정하였다. 결정된 반복 열부하 인가 조건에서 이종금속 접합체가 받는 다른 열팽창 정도에 따른 응력을 계산하여 목업의 수명을 도출하였고, 이를 시험해야 할 반복 횟수로 결정하였다. 각 열부하 조건에 따른 온도조건을 ANSYS-mechanical 코드를 활용하여 열팽창과 이에 따른 접합면의 응력분포로 계산하였다. 0.5 및 $1.0MW/m^2$에 대해, 목업이 받는 최대 응력은 334.3 MPa와 588.0 MPa 였으며, 이 때 텅스텐과 FM강이 받는 strain을 도출하여 물성치로 알려진 cycle to failure 값을 도출하였다. 열부하에서 예상되는 수명은 0.5 및 $1.0MW/m^2$에 대해, 100,000 사이클 이상과 2,655 사이클로 계산되었으며, 시간적 제약을 고려 최종 평가는 $1.0MW/m^2$에 대해, 3,000사이클 정도의 실험을 통해 그 수명까지 접합건전성이 유지되는 지 실험을 통해 평가할 예정이다.

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Prevalence and Toxin Characteristics of Microorganism on Hand Towels Using for Children in Child Care Center (보육시설 유아 사용 수건의 미생물 분포 및 독소 특성)

  • Kim, Jung-Beom;Kim, Nan-Yong;Kang, Suk-Ho;Do, Young-Sook;Eom, Mi-Na;Yoon, Mi-Hye;Lee, Jong-Bok
    • Journal of Food Hygiene and Safety
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    • v.28 no.2
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    • pp.138-145
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    • 2013
  • This study was conducted to evaluate the microbiological contamination on commonly used hand towels in the child care centers and to investigate the toxin gene and toxin production ability of food-borne pathogens. A total of 22 commonly used hand towels including 7 for before use and 15 for during use were tested. The average number of total aerobic bacteria and fungi were 6.2 log CFU/100 $cm^2$ and 4.1 log CFU/100 $cm^2$. Coliform bacteria were detected in 4 out of 7 before used towels (57.1%) and all of during used towels (100%). These results showed that the sanitary conditions of hand towels in the child care centers should be improved promptly. Among the pathogenic bacteria, Staph. aureus and B. cereus without Salmonella spp. were detected in 5 (22.7%) and 11 (50.0%) out of 22 hand towels. All of Staphy. aureus isolated in this study did not possess any toxin genes and did not produce enterotoxin. The detection rate of hblC, hblD, and hblA toxin genes in B. cereus was 72.7, 72.7, and 54.5% respectively. The possession rate of nheA, nheB, and nheC toxin genes showed 81.8, 72.7, and 54.5% respectively. The cytK and entFM toxin genes were presented at 45.5 and 90.0% in B. cereus. The HBL was detected in 8 out of 11 B. cereus isolates (72.7%) and 5 B. cereus isolates produced NHE (45.5%). Ten out of eleven B. cereus isolates (90.9%) produced one or more enterotoxin such as HBL and NHE. From the results, using a private hand towel or paper towel is required to prevent the cross-contamination between commonly used hand towel and children's hands in the child care center.

Prevalence of Microbiological Contamination in the Ready-To-Eat Side Dishes Sold in Gyeongsangnam-do, South Korea (경남지역에서 유통되는 즉석 반찬류의 미생물 오염도 조사)

  • Ji-Yeon Um;Hye-Jeong Jang;Yeon-Ju Choi;So-Young Kim;Areum Jo;Min Young Kim;Jihee Ahn;Jea-Dong Kim
    • Journal of Food Hygiene and Safety
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    • v.38 no.4
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    • pp.217-227
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    • 2023
  • The consumption of ready-to-eat side dishes is rapidly growing in South Korea. These foods are particularly vulnerable to microbiological contamination as they are often cooked without any treatment, such as heating or stored at room temperature after cooking. Hence, in 2022, we analyzed the ready-to-eat side dishes sold in Gyeongsangnam-do, South Korea for microbiological contamination. We collected 100 samples from supermarkets in 7 cities, and then examined them for presence of food-borne pathogens and sanitary indicator bacteria. In the analysis of the food-borne pathogens, Bacillus cereus and Clostridium perfringens were isolated from 51 samples (51.0%) and 3 samples (3.0%), respectively. However, both quantitatively met the Korean Food Standards Codex. Genes of five different enterotoxins and one emetic toxin were analyzed from the 51 isolated B. cereus strains. We detected enterotoxin entFM (100.0%), nheA (94.1%), hblC (58.8%), cytK (56.9%), and bceT (41.2%) in 51 isolates, and emetic toxin gene, CER, in only one (2.0%) isolate. We did not detect C. perfringens toxin gene (cpe) that causes food poisoning in any one of the three C. perfringens isolates. In the case of sanitary indicator bacteria, Kimchi had the highest levels of total aerobic bacteria and coliforms, followed by Saengchae, Jeotgal, Jeolim, Namul, and Jorim, respectively. We counted total aerobic bacteria at two different storage temperatures (4℃ and 20℃) to determine the effect of storage temperature. When stored at 20℃, total aerobic bacteria count increased in most of the ready-to-eat side dishes, except for Jeotgal. This result conclusively shows the need for refrigerating the ready-to-eat side dishes after purchase. Further research is needed to assess the risk and safety of the ready-to-eat side dishes available in the market and determine appropriate safety management practices.