• 약학회지
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• 제42권2호
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• pp.149-152
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• 1998

A high performance liquid chromatographic method was developed for the determination of DA-6034 in biological fluids using internal standard. Plasma containing DA-6034 and inter nal standard was extracted by liquid-liquid extraction at an acidic pH. After evaporation of the organic layer, the drug and internal standard were reconstituted with mobile phase and injected into the column. They were separated by high performance liquid chromatography on inertsil ODS II column at 334 nm. The detection limit of DA-6034 in plasma was 0.02 ${\mu}$g/ml. In this method, the range of recovery and coefficients of variation were 96-110% and 0.40-3.78%, respectively. There was no interference from endogenous substances. Urine and bile were analysed using the deproteinization method and the detection limit of DA-6034 was 1${\mu}$g/l.

• 농업생명과학연구
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• 제51권2호
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• pp.47-59
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• 2017

본 연구의 목적은 박과와 가지과 채소 종자들의 항산화력과 총 페놀 및 플라보노이드들의 함량 변이를 조사하여 고 함유 바이오 활성 화합물 채소 유전자원들을 선발하고, 이를 활용한 고부가가치기능성 상품 개발을 위한 과학적 기초 자료를 제공하는데 있다. 박과(수박, 호박, 여주와 수세미) 및 가지과(매운 고추, 단고추와 가지) 총 408 유전자원들에 대하여 항산화력과 총 페놀 및 플라보노이드들의 함량을 조사하였다. 수박과 호박 종자의 총 페놀 함량은 각각 19.9-343.8 및 $6.1-81.2mg{\cdot}100g^{-1}\;DW$이며, 수박 유전자형의 총 페놀 함량이 $160mg{\cdot}100g^{-1}\;DW$이상인 유전자원은 12%이었다. 수박과 호박 종자의 플라보노이드 함량의 각각 80 및 $41.3mg{\cdot}100g^{-1}\;DW$이었다. 매운 고추 종자의 플라보노이드 함량은 $64.4-472.5mg{\cdot}100g^{-1}\;DW$였고, 평균은 $165.0mg{\cdot}100g^{-1}\;DW$이었다. 23개의 매운 고추 계통이 90% 항산화력이 있는 것으로 분류되었다. 단고추 종자의 항산화력은 35.9-90.3%이며, 항산화력이 82%이상인 유전자원은 23%였다. 가지의 총 페놀과 플라보노이드 함량은 각각 $38.1-642.0mg{\cdot}100g^{-1}\;DW$과 $14.2-1217.0mg{\cdot}100g^{-1}\;DW$이며, 항산화력, 총 페놀 및 플라보노이드 함량이 함께 우수한 8개의 가지 유전자원을 선발하였다. 박과와 가지과 유전자원들의 종자에서 항산화력 및 생리 활성 화합물 함량의 변이가 높았다. 그리고 생리 활성 화합물 함량 및 항산화력이 우수하여 선발된 유전자원들의 종자를 활용하여 기능성 부가가치 상품들을 개발하는데 더 효율적으로 활용될 것으로 기대된다.

• Journal of Plant Biotechnology
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• 제47권4호
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• pp.273-282
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• 2020

In Vietnam, Celastrus hindsii Benth, a medicinal plant rich in secondary metabolites, has been used to alleviate distress caused by ulcers, tumors, and inflammation for generations. The occurrence of two phenotypes, Broad Leaf (BL) and Narrow Leaf (NL), has raised questions about the selection of appropriate varieties for conservation and crop improvement to enhance medicinal properties. This study examined molecular differences in C. hindsii by comparing protein profiles between the NL and BL types using 2D-PAGE and MS. Peptide sequences and proteins were identified by matching MS data against the MSPnr100 databases and verified using the MultiIdent tool on ExPASy and the Blast2GO software. Our results revealed notable variations in protein abundance between the NL and BL proteomes. Selected proteins were confidently identified from 12 protein spots, thereby highlighting the molecular variation between NL and BL proteomes. Upregulated proteins in BL were found to be associated with flavonoid and amino acid biosynthesis as well as nuclease metabolism, which probably attributed to the intraspecific variations. Several bioactive proteins identified in this study can have applications in cancer therapeutics. Therefore, the BL phenotype characterized by healthier external morphological features has higher levels of bioactive compounds and could be better suited for medicinal use.

• 한국작물학회지
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• 제41권spc1호
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• pp.46-68
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• 1996

The objective of this report is to review the up to present papers including 79 references about functional ingredients in maize and their variations. Maize is a good source of such antioxidants as ${\alpha}-,\;{\gamma}-tocopherols,\;{\alpha}-,\;{\beta}-carotene,\;{\beta}-cryptoxanthin$, zeaxanthin, quercetin and chlorogenic acid. Unsaponifiable standard extracts of Zea mays L. (ZML) have a beneficial effect on various dental diseases. Flavonoids, flavonoid biosynthetic pathway and their controling genes were also reviewed in this report. Flavonoids are required for successful fertilization in maize pollen, and maysin has a potent antibiotic activities to earworm and fall armyworm in maize silks. Maize silks have been used in traditional medicine for diuretic, vascular diseases and diabetes, etc. and boiling water extracts of maize silk have hemodynamic effects, but their ingredients still remain obscure.

• Journal of Plant Biology
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• 제35권4호
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• pp.385-392
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• 1992

여뀌속 Tovara절 식물은 매우 복잡한 양상의 형태변이를 나타내기 때문에 각 분류군의 한계 및 위치 성정, 계통적 유연관계 해석에 있어 많은 혼란이 나타나고 있다. 따라서 본 연구에서는 본 절 분류군에서 나타나는 형태 변이의 양상을 수리분류학적으로 분석하여, 각 분류군의 한계를 명확히 설정하고 그 분류학적 타당성을 검토하고자 하였다. 본 절 분류군의 주요 식별형질에 대한 주성분 분석을 수행한 결과, 본 절은 형태적으로 크게 P. virginianum, P. filiforme, P. neofiliforme의 3분류군으로 구분되며, 이들은 주로 잎의 형태에 의해 식별되는 것으로 밝혀졌다. 또한 P. virginianum var. glaberrimum은 그 분류학적 타당성이 없는 것으로 나타났으며, Tovara (=Polygonum) virginiana var. kachina는 형태적으로 볼 때 P. neofiliforme의 변종으로 취급하는 것이 타당한 것으로 판명되었다. Polygonum virginianum, P. filiforme, P. neofiliforme는 그 flavonoid 조성에 있어서도 명확한 차이를 나타내며, 이러한 결과를 종합해 볼 때 이들은 각각 독립된 종으로 인식하는 것이 타당한 것으로 판단된다.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1987년도 식물생명공학 심포지움 논문집 Proceedings of Symposia on Plant Biotechnology
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• pp.323-347
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• 1987

The R locus of maize in one of several genes that regulate the anthocyanin pigments throughout the body of the plant and seed. The R gene product may regulate pigment deposition by controlling the expression of the flavonoid biosynthetic gene pathway in a tissue-specific manner. To understand the basis for tissue specific regulation and allelic variation at R, the molecular study has been done by cloning a portion of the R complex by transposon tagging with Ac. R specific probe were cloned from the R-nj mutant induced by Ac insertion mutagenesis. From southern analysis of R-r complex using the R-nj probe, the structure of R-r was proposed that R-r containes the three elements, (P)(Q)(S). These elements may organize as the inversion triplication model which (S) sequence was inverted in relation to (P) and (Q). The R-sc derivated from R-mb or R-nj was cloned with R-nj probe, and molecular genetical data showed that R-sc containes tissue specific and tissue nonspecific area, and the sequencing of R-sc are progressed now.

• 한국식생활문화학회지
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• 제36권1호
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• pp.84-91
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• 2021

The oriental plum (Prunus salicina) is one of the most popular fruits in Korea due to its sweet taste and pleasant aroma. The objective of this study was to analyze the quality characteristics and antioxidant activities of a puree made using plums stored at low-temperatures. The quality characteristics were determined by estimating total soluble solids (TSS, °Brix), titratable acidity (TA, % citric acid), TSS/TA ratio, pH, colors, sugar content, and sensory profiles. The antioxidant effects were estimated by total polyphenol and flavonoid content and radical scavenging activity. The results showed that the plum puree containing both the flesh and the peel had markedly higher red coloration (a⁎ value) than the puree without the peel, while yellow coloration (b⁎ value) and lightness (L⁎ value) were lower in the puree without the peel than with the peel. The sensory qualities including the plum taste and flavor, texture as well as overall acceptance showed no significant variation between the samples. Meanwhile, the total polyphenol/flavonoid content and radical scavenging activities were significantly improved by the presence of fruit peel and by frozen storage. These findings suggest that frozen plums could be a suitable ingredient for making a puree and concentrate for the food manufacturing industry.

• 한국작물학회지
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• 제68권2호
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• pp.59-68
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• 2023

본 연구는 국립식량과학원에서 육성한 단옥수수 자식계통의 phytochemical 함량과 항산화 활성을 평가하여 고기능성 단옥수수 품종 육성을 위한 기초 자료로 이용하고자 수행하였다. 1. Carotenoid 함량이 가장 높은 계통은 KSE34 (1239.3±6.4 mg 100 g^-1), flavonoid 함량이 가장 높은 계통은 KSE6(68.6±9.6 mg CE 100 g^-1)으로 나타나 각 계통마다 화합물의 함량이 다양하였다. Phenolic compound 함량은 대부분의 단옥수수 자식계통에서 풍부하게 나타났으며, KSE8과 KSE25가 가장 높은 함량을 가진 계통이었다. 2. DPPH와 ABTS 라디칼 소거능으로 평가한 국내 단옥수수 자식계통의 항산화 활성 중 DPPH 활성은 계통 간에 다양하였으나, ABTS 활성은 차이가 적게 나타났다. KSE30과 KSE6이 각각 108.4±9.6 mg TE 100 g^-1, 104.2±12.8 mg TE 100 g^-1로 가장 좋은 DPPH 활성을 보인 계통이었으며 KSE47, KSE4, KSE21은 낮은 활성을 보였다. 3. 계층 군집 분석과 주성분 분석을 사용하여 단옥수수 자식계통의 농업적 특성을 비교하고 분류하였다. Carotenoid, polyphenol, flavonoid 함량이 높고 항산화 활성이 우수한 4개 계통(KSE6, KSE30, KSE34, KSE40)이 선발되었고, 향후 조합 능력 평가를 거쳐 고기능성 단옥수수 품종 개발에 활용할 것이다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.198-198
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• 2017

Cultivated varieties of sweet potato were from dry texture type to tender texture type on the basis of consumer preferences. There are many differences in the quantity of sweet potato, starch content, pigment, and sugar content depending on the cultivation season and area, even in the same variety. Therefore, in this study, we attempted to establish optimum time of harvesting through growth characteristics and variation in component like starch, sugar, polyphenol and flavonoid. Four sweet potato varieties were used in this experiment. Among them, Jinhongmi (JHM) & Yulmi (YM) were as dry texture type and Pungwonmi (PWM) & Hogammi (HGM) were as tender texture type. Sweet potatoes were transplanted on 23 May, 2016 and were investigated storage root weight and component contents every 20 days from 60 days to 120 days and surveyed yield at 110, 120, 130 days after transplantation. Result revealed that storage root weight of YM, JHM, and HGM were 30.1, 38.9, 20.8 g respectively in 60 days after transplanting. Storage roots of PWM gerw faster with the weight of 88.2 g. In 120 days after transplanting, storage root weight varied from 88.3 to 118.7 g, HGM was the smallest, and PWM was the largest. Sugar contents of sweet potato ranged from 21.0 to $23.8Brix^{\circ}$ in 60 days after transplanting and from 27.5 to $30.78Brix^{\circ}$ in 120 days after transplanting. In particular, the sugar content of HGM was the highest over $30Brix^{\circ}$ after 80 days. The starch content of dry texture type (YM, JHM) increased from 15.5% to 20.4% and tender texture type (PWM, HGM) increased from 11.0% to 17.3%. Starch content tended to be high in dry type sweet potatoes. The content of polyphenol and flavonoid were highest in 60 days after transplanting and was reduced according to cultivation period. The total yield of PWM was high as 3,154 kg/10a and large storage root of over 250 g accounted for 47.4% in 110 days after transplanting. Storage root (YM, JHM, HGM) of 81~150 g accounted for 34.9% ~ 43.2% in 120 days after transplanting. These are the most marketable. Because consumer in Korea prefers small, round and about 100g size sweet potato. The ratio of large storage root (over 250 g) were increased in all varieties at 130 days after transplanting. Therefore, it is considered appropriate to harvest PWM at 110 days and YM, JHM, HGM at 120 days after transplanting, which planted in late May.

• 한국작물학회지
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• 제41권spc1호
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• pp.123-144
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• 1996

This review deals briefly with the various medicinal components(mainly saikosaponins), their biological activities and the variation of their contents by different cultivation environment and plant parts in Bupleurum species. Bupleuri radix, a crude drug, is the root of Bupleurum falcatum L. (Korea, Japan), B. chinense(China), and their related species (Umbelliferae). There are over 120 species in Bupleurum genus throughout world, mainly Asian area, and over 5 species in Korea, investigated up to now. These plants contain many physiological active compounds and the principal components are saikosaponins. Major activities of this crude drug and saikosaponins are the anti-inflammatory and antihepatotoxic activities. Saikosaponins and their derivatives in Bupleurum spp. have been chemically studied, isolated and identified over 70 compounds in over 50 species. Other components, physiologically active ones, also have been investigated, which are the groups of lignan, flavonoid, essential oil, polyacetylene, polysaccharide, etc. Saikosaponins belong to the group of triterpenoid saponin chemotaxonomically and occur the accumulation and turnover in plant tissues through secondary metabolism, mevalonic acid pathway. The contents and kinds of saikosaponins and other components in Bupleurum spp. plants are various due to different species and growing environments, as the plant growth characters and yield are various. Most of medicinal plants as well as Bupleurum species are very useful as agricultural products and traditional medicines, and also are very valuable as genetic resources and natural products. So we need to collect, evaluate, preserve, and utilize various medicinal plants, and also to under-stand secondary metabolism and improve the breeding and cultivation techniques for the safe production of crude drugs with high quality and yielding.