• The Bulletin of The Korean Astronomical Society
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• v.35 no.2
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• pp.47.2-47.2
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• 2010

태양플레어는 태양 대기에서 발생하는 격렬한 폭발현상으로 이를 예측하고 대비하기가 쉽지 않다. 본 연구에서는 플레어의 발생 확률이 태양 흑점 분류와 흑점 면적 변화량에 어떻게 의존하는 가를 조사하였다. 이를 위하여 약 9년 기간(2001년 7월 ~ 2010년 6월) NOAA에서 제공하는 AR(Active Region) 정보에 근거한 McIntosh 흑점 분류법을 사용하였다. 플레어는 C 등급 이상(C,M,X)인 것만을 고려하였다. 본 연구에서는 60개의 McIntosh 흑점군 그룹 중 가장 플레어를 많이 발생시키는 6개의 흑점군 그룹에 대해 태양 흑점 면적의 변화량을 각각 3그룹으로 나누어(감소, 무변화, 증가) 비교해보았다. 그 결과 거의 모든 그룹에서 태양 흑점의 넓이가 증가, 감소, 무변화 순으로 플레어의 발생 확률이 높다는 것을 확인하였다. 예로, 흑점군 그룹 중 Fkc그룹의 경우 위의 순서대로 65%, 50%, 44%로 M등급의 플레어가 발생했다. 흑점의 면적 변화가 자기플럭스의 변화를 나타내는 좋은 인자임을 고려할 때, 본 결과는 새로운 자기플럭스가 광구로 상승하는 경우에 플레어의 발생 확률이 더 높음을 보여준다. 본 연구 결과를 토대로 태양 흑점 분류와 면적의 변화량에 따른 플레어 발생 확률 연구의 발전방향과 활용 방안에 대해 논의하고자 한다.

• Journal of fish pathology
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• v.8 no.2
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• pp.119-134
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• 1995

To induce an overpopulated melanomacrophage centers(MMCs) within spleen and kidney in tilapias, two methods were applied through the intraperitoneal inoculation of V. anguillarum FKC with a repeated dose of saline(the 1st induction group), and of colloidal carbon (the 2nd induction group). In the 1st group, both number and size of MMCs were slightly increased in spleen and head kidney. However in the 2nd group the two hemopoietic tissues were nearly occupied with quite a large number of MMCs. Regardless of induction groups, many of MMCs were confined within the walls of blood vessels in the spleen. Especially in the 2nd group, the MMCs without fibrous capsules often had concentrically or eccentrically located, thin-walled lumens of vessels, which strongly suggests to be ellipsoids. In head kidney, nearly all MMCs were located within or just around the lymphocytic areas but the precise relationship to blood vessel wall was not obvious. Despite of such overpopulated MMCs, no histopathological degenerative findings in hemopoetic parenchymas of both organs were recognized. To evaluate the effect on defensive function, tilapias of the 2nd group were challenged with E. tarda. Susceptabilities to E. tarda were never increased but rather significantly decreased compared to control. Weekly antibody titres in sera were determined for all induction groups, in which the titres in the 1st and 2nd groups were 4 or 8 times higher than in the control, and then remained high until the 4th week. Also with the hemopoietic function, cellular compositions of peripheral blood were analyzed at weekly intervals but no significant changes resulted. From those results, it is suggested that overcrowding of MMCs would not lead to any morphological as well as functional deteriorations of spleen and head kidney but have an intimate association with enhancement of protective immune system in tilapias.

• Journal of fish pathology
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• v.10 no.1
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• pp.45-52
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• 1997

A protein-bound polysaccharide preparation, PS-K, isolated from Coriolus versicolor was evaluated for its ability to enhance resistance against Edwardsiella tarda, causal agent of classical edwardsiellosis in tilapia, Oreochromis niloticus. Fish treated with a dose rate of 0.1 mg PS-K $g^{-1}$ body weight were challenged by intraperitoneal injection of E. tarda with different concentrations. Against low burdens of injections($1.2{\times}10^7$ and $1.2{\times}10^8$ cfu/fish), PS-K treated fish did not show any mortality compared to 50% and 100% mortality of control groups, respectively. However there was no increase of resistance against challenge with high concentrations of E. tarda ($1.2{\times}10^9$ and $1.2{\times}10^{10}$cfu /fish) except few days delaying of death. Tilapia injected with PS-K one day after intraperitoneal inoculation of E tarda($1.2{\times}10^7$ cfu/fish) showed 100% survival rate compared to control group of 50% survival rate. The result indicates the potential of PS-K as a prophylactic agent in aquaculture. The increased antibody response in fish received PS-K one week prior to FKC administration suggested the influence of PS-K on the specific humoral immunity and increased resistance against bacterial infection.

• Journal of fish pathology
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• v.14 no.2
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• pp.97-102
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• 2001

Olive flounder, Paralichthys olivaceus known as an one of the major aquacultured species in Korea were exposed to cadmium(Cd) with different protocols and analyzed the effects of exposure on the immune response. Antibody levels in sera of the group exposed to Cd(20ppb) by immersion method from 2 weeks before immuniztion with formalinised Edwardsiella tarda(E. tarda) KFE entigen to the end of experiment reached to peak level faster than that of the non-exposed group. After this peaking time the levels decreased much at a faster rate compared to the non-exposed group. This tendency was also appeared in the numbers of specific antibody secreting cells(SASC) analyzed with the enzyme-linked immunospot (ELISPOT)-assay technique in the splenocytes of the experimental groups exposed to Cd with different ways. Interestingly, the group exposed to Cd for 2 weeks before immunization also showed increased numbers of SASC unlikely the antibody production and suggested a more critical influence of cadmium exposure in early stage of immune reaction. Artificial infection with live E. tarda KFE induces 100% mortality in the flounder exposed to cadmium throughout the experimental period from two weeks before the immunization. It may imply that some other factors related to specific immunity are involving in the defence system of flounder exposed to Cd. Taked together. Cd exposure may induce temporaily stimulatory or indhibitory effects on the immune reaction, but suppress the physiological systems for the resistant against the infective agents with other toxic effects.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.5
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• pp.620-628
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• 1996

To avoid the self-agglutination of Staphylococcus aureus sensitized with rabbit antibody in the absence of antigen, we determined the optimum concentration of rabbit antibody for sensitization. It was analyzed by using three different kinds of S. aureus strains at various concentraions of antibody. The optimized coagglutination test using the S. aureus sensitized with rabbit antibody was applied to the diagnosis of edwardsiellosis in field and in laboratory. The presence of E. tarda as low as $10\;{\mu}g/ml$ was detected by this method. Moreover, it showed good coagglutination results against several different forms of antigens such as FKC, EDTA or heat extracted antigen of E. tarda. E. tarda strains, isolated from the flounders suffering from edwardsiellosis in fields, showed some cross-reactions to the E. tarda 219 analyzed by both agglutination and coagglutination test with rabbit anti-E, tarda 219 antibody. The degree of cross-reactions analyzed was enough to apply the coagglutination test for the diagnosis of edwardsiellosis in field. Thus, even 1,000 fold diluted tissue homogenate of infected flounder naturally contained enough E. tarda as an antigen to show good coagglutination with S. aueus sensitized with rabbit anti-E, tarda 219 antibody. The successful application of this method to the homogenate and heat extract of tissues from naturally or artificially infected flounder or tilapia preyed that coagglutination test was a simple and rapid reliable dignostic technique suitable for using in laboratory and field without any special equipments.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.1
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• pp.88.2-88.2
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• 2012

We investigate solar flare occurrence rate and daily flare probability depending on McIntosh sunspot classification, its area, and its area change. For this we use the NOAA active region and GOES solar flare data for 15 years (from January 1996 to December 2010). We consider the most flare-productive 10 sunspot classification: 'Dko', 'Dai', 'Eai', 'Fai', 'Dki', 'Dkc', 'Eki', 'Ekc', 'Fki', and 'Fkc'. Sunspot area and its change can be a proxy of magnetic flux and its emergence/cancellation, respectively. we classify each sunspot group into two sub-groups: 'Large' and 'Small'. In addition, for each group, we classify it into three sub-groups according to sunspot group area change: 'Decrease', 'Steady', and 'Increase'. As a result, in the case of compact groups, their flare occurrence rates and daily flare probabilities noticeably increase with sunspot group area. We also find that the flare occurrence rates and daily flare probabilities for the 'Increase' sub-groups are noticeably higher than those for the other sub-groups. In case of the (M+X)-class flares of 'Dkc' group, the flare occurrence rate of the 'Increase' sub-group is three times higher than that of the 'Steady' sub-group. Mean flare occurrence rates and flare probabilities for all sunspot regions increase with the following order: 'Steady', 'Decrease', and 'Increase'. Our results statistically demonstrate that magnetic flux and its emergence enhance major solar flare occurrence. We are going to forecast solar flares based on these results and NOAA scale.

• The Bulletin of The Korean Astronomical Society
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• v.36 no.2
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• pp.98-98
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• 2011

We have investigated solar flare probability depending on sunspot classification, its area, and its area change using solar white light data. For this we used the McIntosh sunspot groups with most flare-productive regions : DKI, DKC, EKI, EKC, FKI and FKC. For each group, we classified it into three sub-groups according to sunspot area change : increase, steady, and decrease. For sunspot data, we used the NOAA active region information for 11 years (from January 2000 to December 2010): daily sunspot class and its area corrected for the projection effect. As a result, we find that the mean flare rates and the flare probabilities for the "increase" sub-groups are noticeably higher than those for other sub-groups. In case of the (M+X)-class flares of 'kc' groups, the mean flare rates of the "increase" sub-groups are more than two times than those of the "steady" sub-groups. This is statistical evidence that magnetic flux emergence is an very important for triggering solar flares since sunspot area increase can be a good proxy of magnetic flux emergence. In addition, we have examined the relationship between sunspot area and solar flare probability. For this, we classified each sunspot group into two sub-groups: large and small. In the case of compact group, the solar flare probabilities noticeably increase with its area.

• The Bulletin of The Korean Astronomical Society
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• v.39 no.1
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• pp.76.1-76.1
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• 2014

We investigate the solar flare and CME occurrence rate and probability depending on sunspot class and its area change. These CMEs are front-side, partial and full halo CMEs associated with X-ray flares. For this we use the Solar Region Summary(SRS) from NOAA, NGDC flare catalog, and SOHO/LASCO CME catalog for 16 years (from January 1996 to December 2011). We classify each sunspot class into two sub-groups: "Large" and "Small". In addition, for each class, we classify it into three sub-groups according to sunspot class area change: "Decrease", "Steady", and "Increase". In terms of sunspot class area, the solar flare and CME occurrence probabilities noticeably increase at compact and large sunspot groups (e.g., 'Fkc'). In terms of sunspot area change, solar flare and CME occurrence probabilities for the "Increase" sub-groups are noticeably higher than those for the other sub-groups. For example, in case of the (M+X)-class flares of 'Dkc' class, the flare occurrence probability of the "Increase" sub-group is three times higher than that of the "Steady" sub-group. In case of the 'Eai' class, the CME occurrence probability of the "Increase" sub-groups is five time higher than that of the "Steady" sub-group. Our results demonstrate statistically that magnetic flux and its emergence enhance solar flare and CME occurrence, especially for compact and large sunspot groups.

• The korean journal of orthodontics
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• v.26 no.6
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• pp.667-676
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• 1996

We have examined the in vitro stage-related chondrogenic potential of human mandibular and limb bud mesenchyme cells using micromass culture. Our results indicate that limb bud mesenchyme cells as early as stage 16 by Carnegie system (37 days), well before the initiation of in vivo chondrogenesis, have chondrogenic potential which is expressed in micromass culture. These results are correlated with stage-related chondrogenic potential of human limb bud in vivo as a result of Alcian blue staining. The proliferation of chondrogenic cells increased in the first 3 days after culture and then decreased. These results were correlated with the cell cycle analysis of which the number of $G_0^1/G_1$ phase increased markedly after 3 days of culture, while the percentage of cells in S phase was decreased. On the other hand, it was rarely differentiated in the mandible. We examined the effects of two PKC modulators such as phorbol 12-myristate 13-acetate (PMA), a potent activator of PKC, and staurosporine (STSN), an inhibitor of PKC. PMA inhibited the chondrogenesis, whereas STSN promoted the chondrogenesis in a dose dependent manner. In addition, PMA exerted no inhibitory effect when the cells were pretreated for 24 h with STSN, implying that the chondrogenic events might be settled at an early step in vitro and FKC may act as a negative modulator. Collectively, these results demonstrate, for the first time, the stage-related chondrogenic potential of human mandibular and limb bud mesenchyme cells and the role of PKC during chondrogenesis in vitro & in vivo.

• Journal of fish pathology
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• v.22 no.1
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• pp.23-33
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• 2009

Vibrio harveyi was a significant pathogenic agent and cause a high mortality of cultured fish and shrimp in the aquaculture industry. In this study, we have investigated biochemical, physiological, serological and immunological characteristics of V. harveyi isolated from marine cultured fish. The phenotypes of V. harveyi were differentiated with their own biochemical characteristics and colors of colony on the TCBS agar. V. harveyi were classified into more than four serogroups by agglutination test. Most isolates were classified into a group A which is categorized with the same band pattern generated by western blotting. Group A was characterized by a major protein, which is ranged from 26 and 34 kDa in size, and has a virulence to oliver flounder more than reference strain KCCM40866. Oliver flounders vaccined with FKC of V. harveyi C05011 were highly resistant to infection by other strains of group A.