• 약학회지
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• 제42권6호
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• pp.567-571
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• 1998

The phagocytic activity of murine peritoneal macrophage, was determined by lucigenin chemiluminescence and engulfment of fluorescein-conjugated E. coli particle. The acti vity-guided fractionation upon the methylenechloride fraction of Aurantii immaturi pericarpium led to the isolation of a flavonoid, isosinensetin, as a suppressive component of phagocytosis. Isosinensetin suppressed the lucigenin chemiluminescence and the engulfment of fluorescein-conjugated E. coli particles and enhanced the production of nitric oxide in murine peritoneal macrophage.

• 사상체질의학회지
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• 제13권3호
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• pp.102-113
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• 2001

The purpose of this research was to investigate the effects of Seungyangikkitang (SIT) on the immune cells in BALB/c mice. SIT (500mg/kg) was administerd p.o. once a day for 7 days. SIT enhanced the proliferation of thymocytes, but decreased the proliferation of splenocytes. SIT enhanced the subpopulation of cytotoxic T cells in thymocytes and helper T cells in splenocytes, but did not affect the subpopulation of B220/Thy1 cells. SIT enhanced the production of γ-interferon and interleukin-2 in thymocytes, splenocytes and serum, but did not affect the production of interleukin-4. SIT suppressed the production of nitric oxide, but enhanced the lucigenin chemiluminescence and the engulfment of FITC-conjugated E. coli particles in peritoneal macrophages. These results suggest that SIT has a potent activity on the specific immunity via the cytokine secretion of Th1 cells and the non-specific immunity via the phagocytic activity of macrophages in vivo.

• The Plant Pathology Journal
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• 제26권1호
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• pp.57-62
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• 2010

Low-molecular-weight secondary metabolites from plants play an important role in reproductive processes and in the defense against environmental stresses or pathogens. In the present study, we isolated various volatiles and phenolic compounds from white Rosa rugosa flowers, and evaluated the pharmaceutical activities of these natural products in addition to their ability to increase survival in response to environmental stress and pathogen invasion. The DPPH and hydroxyl radical-mediated oxidation assay revealed that the white rose flower extract (WRFE) strongly scavenged free radicals in a dose dependent manner. Moreover, WRFE inhibited the growth of E. coli and fatally attacked those cells at higher concentration (>0.5 mg/mL). FITC-conjugated Annexin V stain provided further evidence that WRFE had strong antimicrobial activity, which may have resulted from a cooperative synergism between volatiles (e.g. 1-butanol, dodecyl acrylate and cyclododecane) and phenolic compounds (e.g. gallic acid) retained in WRFE. In conclusion, secondary metabolites from white rose flower hold promise as a potential natural source for antimicrobial and non-chemical based antioxidant agents.

• Nuclear Medicine and Molecular Imaging
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• 제43권5호
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• pp.487-494
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• 2009

목적: 작은 크기의 재조합 단일사슬 항체는 빠른 혈중 제거율과 종양의 항체 집적율이 증가되는 등의 장점을 가지고 있다. 반면에 항체의 작은 크기는 방사성 또는 형광물질의 표지를 위한 킬레이터 결합에 중요한 아미노산 그룹의 감소를 의미하기도 한다. 본 연구에서는 단일사슬 lym-1 염기서열 C-말단에 lysine 아미노산 태그를 삽입하여 형광 물질의 직접표지 및 그 표지수율 증가를 확인하고자 하였다. 대상 및 방법: 대장균 pET-22b (+) 벡터에 재조합 된 lysine 삽입 단일사슬 lym-1유전자는 대장균 BL21 (DE3)에 형질전환하여 발현하였다. 생산된 lysine lym-1 항체는 Ni-NTA 컬럽과 분자량 컬럼을 사용해 정제하였고. 단백질 전기 영동과 western blot을 통해 확인하였다. lysine lym-1 항체에 방사성 동위원소인 I-124, I-125, I-131 과 Tc-99m를 표지하여 그 수율을 확인하였으며 유세포계측기를 사용해 형광물질인 FITC가 직접표지된 라이신 lym-1 항체의 면역반응성을 사람의 버킷 림프종 세포주인 Raji 세포주에서 면역반응성을 확인하였다. 결과 Lysine도입 단일사슬 lym-1 항체는 두 과정의 정제를 통하여 획득하였으며 그 크기는 약 48 KDa이었고, 방사성동위원소인 I-124, I-125, I-131과 Tc-99m의 표지수율은 각각 >99%, >99%, >95%, >99%로 확인되었다. 유세포계측을 통한 lysine 도입 단일사슬 lym-1항체의 면역반응성은 기존의 단일사슬 lym-1항체와 유사함을 확인하였다. 결론: 재조합 lym-1 항체에 형광 물질을 직접 표지하기 위한 lysine 아미노산의 도입은 항체의 면역반응성 감소를 최소화 시키면서 직접표지 수율을 증가시킬 수 있는 유용한 방법임을 확인하였다.