• 제목/요약/키워드: FIA

검색결과 102건 처리시간 0.026초

푸마르산의 온라인 모니터링을 위한 흐름주입분석 기술 개발

  • 손옥재;이종일
    • 한국생물공학회:학술대회논문집
    • /
    • 한국생물공학회 2002년도 생물공학의 동향 (X)
    • /
    • pp.136-138
    • /
    • 2002
  • In this work we describe the on-line monitoring technique for the analysis of fumaric acid in biotechnological processes. Fumarase and malate dehydrogenase(MDH) were immobilized on epoxy carrier and integrated into a FIA system. The effects of carrier buffer flow rate, pH, reaction temperature on the immobilized fumarase/MDH were investigated for the development of a fumarate-FIA system. Furthermore the effects of substrates, salts and metabolites dissolved in the sample on the activity of the immobilized enzyme were investigated.

  • PDF

Short-term improvement of masticatory function after implant restoration

  • Kang, Si-Mook;Lee, Sang-Soo;Kwon, Ho-Keun;Kim, Baek-Il
    • Journal of Periodontal and Implant Science
    • /
    • 제45권6호
    • /
    • pp.205-209
    • /
    • 2015
  • Purpose: Dental implants present several advantages over other tooth replacement options. However, there has been little research on masticatory function in relation to implant treatment. Therefore, the aim of the present study was to evaluate the improvement of masticatory function two weeks after implant restoration. Methods: Masticatory ability was evaluated with the subjective food intake ability (FIA) and objective mixing ability index (MAI) methods. Fifty-four subjects with first and second missing molars completed the study. The subjects were asked to complete a self-reported questionnaire about 30 different food items, and to chew wax samples 10 times both before and two weeks after implant restoration. A total of 108 waxes were analyzed with an image analysis program. Results: Dental implant restoration for lost molar teeth on one side increased the FIA score by 9.0% (P<0.0001). The MAI score also increased, by 14.3% after implant restoration (P<0.0001). Comparison between the good and poor mastication groups, which were subdivided based on the median MAI score before implant restoration, showed that the FIA score of the poor group was enhanced 1.1-fold while its MAI score was enhanced 2.0-fold two weeks after an implant surgery. Conclusions: Using the FIA and MAI assessment methods, this study showed that masticatory function was improved two weeks after implant restoration. In particular, the enhancement of masticatory function by implant restoration was greater in patients with relatively poor initial mastication than in those with good initial mastication.

한지자숙폐액을 이용한 Saccharomyces cerevisiae의 배양에서 온-라인 FIA시스템에 의한 Glucose의 자동분석 및 첨가에 의한 증균 (Increase of Cell Concentration by the Automatic Analysis and Addition of glucose with an On-line Flow Injection Analysis System int he Cultivation of Saccharomyces cerevisiae Using a Korean Paper Digestion Wastewater)

  • 이형춘
    • KSBB Journal
    • /
    • 제15권4호
    • /
    • pp.388-392
    • /
    • 2000
  • 한지자숙폐액을 이용한 Saccharomyces cerevisiae의 배양에 서 glucose의 자동분석 및 첨가에 의해 균농도를 증가시킴으로써 폐액의 재이용성을 향상시키파 하였다 배양액의 glucose를 자동분석하고 자동첨가하기 위하여 ceramic sampler, sampling, ralve, injection valve, glucose oxidase column, debubbler, flow cell과 백금전극, potentiostat, computer와 interface card, 및 tubing pump블로 구성된 glucose 자동분석 및 첨가시스템을 제작하였으며, glucose의 자동분석에는 glucose oxidase를 이용한 on-line FIA법을 채택하였다. 효모의 배양중 glucose자 동분석 및 첨가시스템을 사용하여 glucose를 자동첨가한 결과 배양액의 포도당농도가 $176{\pm}31 mg/L$ 의 낮은 농도로 제어 되었으며, glucose와 $>(NH_4)_2S0_4$의 첨가에 의해 총균수가 3.1 배 증가하였다.

  • PDF

The National Forest Inventory of the United States of America

  • McRoberts, Ronald E.
    • Journal of Forest and Environmental Science
    • /
    • 제24권3호
    • /
    • pp.127-135
    • /
    • 2008
  • The mission of the Forest Inventory and Analyis (FIA) program of the Forest Service, U.S. Department of Agriculture, is to conduct the national forest inventory of the United States of America for purposes of estimating the area of forest land; the volume, growth, and removal of forest resources; and the health of the forest. Users of FIA data, estimates, and related products include land managers, policy and decision-makers, forest industry, environmental organizations, and university researchers. To accomplish its mission, the FIA program has established a sampling design with an intensity of approximately one permanent plot per 2,400 ha across the entire country. Depending on the region of the country, each plot is remeasured at intervals of five or 10 years. The program releases data annually and reports estimates at the county level for each state every five years. Due to budgetary constraints and natural variability among plot observations, sufficient numbers of plots cannot be measured to satisfy precision guidelines for the estimates of many variables unless the estimation process is enhanced using ancillary data. Classified satellite imagery has been demonstrated to be a source of ancillary data that can be used with stratified estimation techniques to increase the precision of estimates with little corresponding increase in costs. A crucial factor restricting the utility of FIA data is that the exact locations of inventory plots cannot be released to the public. Thus, users are generally not able to obtain estimates for small areas or for their own areas of interest if exact plot locations are required. To compensate, satellite imagery, inventory plot data, and the k-Nearest Neighbors technique are being used to construct Internet-based maps of forest attributes from which estimates for arbitrary user-defined areas of interest may be obtained.

  • PDF

알콜증류폐액을 이용한 빵효모배양에서 Glucose와 Ammonium의 자동첨가에 의한 증균 : 온라인 FIA 시스템에 의한 Ammonium의 자동분석 및 제어 (Increase of Cell Concentration by the Automatic Addition of Glucose and Ammonium to an Alcohol Distillery Wastewater Reutilized for Cultivating a Baker's Yeast : Automatic Analysis and Control of Ammonium Concentration with an On-line Flow Injection Analysis System)

  • 이형춘
    • KSBB Journal
    • /
    • 제15권2호
    • /
    • pp.139-144
    • /
    • 2000
  • 알콜증류폐액에 빵효모를 배양하는 중에 glucose와 ammon-ium을 자동첨가하여 균체량을 효율적으로 증가시킴으로써 폐액의 재이용성을 향상시키고자 하였다. Glucose는 DO를 제어파라미터로 하는 제어방법을 사용하여 116 mg/L 이하로 자동제어하였다. Ammonium의 경우에는 FIA를 이용한 온라인 자동계측제어장치와 프로그램을 이용하여 배양액의 ammon-ium 농도를 자동분석하고 제어하였으며, ammonium 농도를 류.0~27.7 mM의 낮은 농도범위로 유지시킬 수 있었다. Ammo-nium 농도를 제어한 본 실험의 최대비증식속도는 0.21 hr-1이었고, 대당균체수율은 약 0.78 g/g 으로서 ammonium 농도를 제어하지 않은 경우에 비하여 대당균체수율은 약 1.4배, 최대비증식속도는 1.2배 더 높아졌으므로 ammonium 제어에 의하여 균의 glucose 이용효율이 더욱 향상되었다. 대암모늄균체수율은 약 11.3 g/g 이었다. Glucose와 ammonium을 첨가함으로써 균체량을 18.5 g/L 까지 높일 수 있었으며, 이는 첨가하지 않은 경우보다 7.1배 더 높은 균체량에 해당한다. Glucose와 ammonium의 농도를 낮게 유지시킬 수 있었으므로 두가지 영양성분의 경제적인 공급이 가능하였다.

  • PDF

발효공정 자동제어를 위한 포도주 발효 중 포도당 농도 온라인 측정 (On-line Monitoring of a Glucose Concentration on a Fermentation Process of Wine for an Automatic Control of a Fermentation Process)

  • 송대빈
    • Journal of Biosystems Engineering
    • /
    • 제33권4호
    • /
    • pp.276-281
    • /
    • 2008
  • A flow injection analysis method (FIA), which analyzes sample conditions after injecting a sample and reagents into a continuous stream, are recognized as the most adequate analyzing method according to the increase of sampling frequency, the decrease of measuring time and the diversity of measuring targets. Specially, the FIA is considered to be used effectively for the control of a fermentation process to produce fermentation food and useful microbial production by activation of a fermentation industry for development of biological materials. In this study, a flow injection analysis sensor unit was developed for on-line monitoring of the fermentation process. The performance was verified by on-line measuring the concentration of glucose of the fermentation process of wine. The glucose concentrations of the samples were measured every 12 hours during the whole fermentation process and compared with those by a HPLC. The concentration relative errors of glucose on the fermentation process of wine showed below 30% within 72 hours and over 50% after the 72 hours. The sensor unit had potential to on-line monitoring of the fermentation process but some problems to overcome for an commercial application.

음성 웹서비스를 위한 VoiceXML 해석기의 설계 및 구현 (Design and Implementation of the VoiceXML Interpreter for Voice Web-service)

  • 신현경;강동남;염세훈;유재우
    • 한국음향학회지
    • /
    • 제20권4호
    • /
    • pp.42-47
    • /
    • 2001
  • 본 연구의 목적은 비 시각환경에서 웹 서비스를 위한 언어인 VoiceXML을 기존의 자동응답 시스템에 적용하기위해 VoiceXML문서의 마크-업을 인식하고, 문서가 문서 형정의 (DTD)에 적합한지를 검사하여 적합성이 확인되면 추상구문트리를 생성하는 DI 파서 (Document Instance Parser)와 생성된 추상구문트리를 이용하여, Voice-XML문서를 번역해주는 해석기를 제안하고자 한다. VoiceXML해석기는 DI 파서와 실행기로 구성되어 있으며, DI 파서는 Recursive descent 파싱 기법을, 실행기는 VXML 포럼에서 제안한 FIA (Form Interpretation Algorithm)를 사용하였다. 본 시스템은 VoiceXML 언어를 효율적으로 실행할 수 있는 환경 제공 및 시스템 개발의 편의성과 효율성을 위해 모듈화 설계가 가능한 자바언어를 사용함으로써 이 기종간의 이식성이 뛰어난 특징이 있다.

  • PDF

On-line Monitoring and Control of Substrate Concentrations in Biological Processes by Flow Injection Analysis Systems

  • Rhee, Jong-Il;Adnan Ritzka;Thomas Scheper
    • Biotechnology and Bioprocess Engineering:BBE
    • /
    • 제9권3호
    • /
    • pp.156-165
    • /
    • 2004
  • Concentrations of substrates, glucose, and ammionia in biological processes have been on-line monitored by using glucose-flow injection (FIA) and ammonia-FIA systems. Based on the on-line monitored data the concentrations of substrates have been controlled by an on-off controller, a PID controller, and a neural network (NN) based controller. A simulation program has been developed to test the control quality of each controller and to estimate the control parameters. The on-off controller often produced high oscillations at the set point due to its low robustness. The control quality of a PID controller could have been improved by a high analysis frequency and by a short residence time of sample in a FIA system. A NN-based controller with 3 layers has been developed, and a 3(input)-2(hidden)-1(output) network structure has been found to be optimal for the NN-based controller. The performance of the three controllers has been tested in a simulated process as well as in a cultivation process of Saccharomyces cerevisiae, and the performance has also been compared to simulation results. The NN-based controller with the 3-2-1 network structure was robust and stable against some disturbances, such as a sudden injection of distilled water into a biological process.

FIA를 이용한 저농도 인산 정량법 개발

  • 신용승;구현빈;최홍복;정형근
    • 한국환경과학회:학술대회논문집
    • /
    • 한국환경과학회 1999년도 임시총회 및 가을 학술발표회 초록집 The Korean Environmental Sciences Society
    • /
    • pp.115-116
    • /
    • 1999
  • PDF

분변내 성호르몬의 분석을 통한 멸종위기 사육 오랑우탄에서의 번식형태 (The Reproductive Patterns of Endangered Captive Orangutans by Analysing the Sex Hormones in Feces)

  • 정소영;김보숙;윤용달
    • 한국임상수의학회지
    • /
    • 제30권1호
    • /
    • pp.22-26
    • /
    • 2013
  • To monitor the reproductive patterns of endangered orangutan, Time-resolved fluorescence immuno assay (TR-FIA) were used to analyze metabolites of sex hormones in feces. Orangutan had long-term interbirth intervals (amenorrhea) during lactation period in which the secretion of sex hormones was repressed. The concentration of progesterone in the serum of pregnant orangutan was 30fold higher than that in non-pregnant orangutan. However, the concentration of hCG during pregnant period was different from the result of other primates. The present study suggested that age is not the important factor in determining the reproduction capability, because it is rather greatly influenced by the combination of various factors. Tracing metabolites of sex hormones in orangutan feces seems to be provide the effective solution for the infertility in orangutan. This study result shows the basic data in operating conservation project for endangered orangutan.