• Title/Summary/Keyword: FDH-H

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Optimized Production of Selenocysteine Containing Formate Dehydrogenase H in Escherichia coli (대장균에서 Selenocysteine을 가지고 있는 Formate Dehydrogenase H의 최적화 생산)

  • Sa, Young-Seung;Kim, Yong-Hwan
    • KSBB Journal
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    • v.26 no.3
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    • pp.189-192
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    • 2011
  • Much interest has been recently focused on the production of large quantities of hydrogen, due to its potential importance in our economy and needs in the petroleum and chemical industries. Formate dehydrogenase H (FDH-H) from Escherichia coli containing selenocysteine that oxidizes formate to carbon dioxide with the release of hydrogen is a component of the anaerobic formate hydrogen-lyase complex of E. coli. To make full use of FDH-H, we need effective expression condition. In this approach, we investigated the effect of pH on FDH-H stability and observed the effect of selenite and formate concentration on the activity of FDH-H. Additionally, coexpression of selenocysteine insertion genes were tried to improve the expression of FDH-H. The highest level of FDH-H expression was achieved by coexpression of selenocysteine insertion genes (pSUABC) as well as by the addition of $10\;{\mu}M$ selenite and 10 mM formate. At this optimized condition, a 2.6 fold elevation of expression of FDH-H was achieved.

Corrosion Behavior of Boiler Tube under Circulation Water Conditions in District Heating System (지역난방 시스템의 순환수에 따른 보일러 튜브의 부식 특성)

  • Hong, Minki;Cho, Jeongmin;Song, Min Ji;Kim, Woo Cheol;Ha, Tae Baek;Lee, Soo Yeol
    • Corrosion Science and Technology
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    • v.17 no.6
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    • pp.287-291
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    • 2018
  • In this study, corrosion behavior of a SA178-A alloy used in the boiler tube of a district heating system was investigated in different environments where it was exposed to pure water, district heating (DH) water, and filtered district heating (FDH) water. After the corrosion test, the surface morphology was examined for observation of the number of pitting sites and pitting area fraction, using a scanning electron microscope. The DH water and FDH water conditions resulted in a lower corrosion potential and pitting potential, and revealed a significantly higher corrosion rate than the pure water condition. The pitting sites in the DH water (pH 9.6) were approximately eighteen times larger than those in the pure water (pH 9.6). Compared to the DH water, the corrosion potential became more noble in the FDH water condition, where iron ions were reduced through filtration. However, the corrosion rate increased in the FDH water due to an increased concentration of chloride ions, which deteriorated the stability of passive film.

Complete In Vitro Conversion of n-Xylose to Xylitol by Coupling Xylose Reductase and Formate Dehydrogenase

  • Jang, Sung-Hwan;Kang, Heui-Yun;Kim, Geun-Joong;Seo, Jin-Ho;Ryu, Yeon-Woo
    • Journal of Microbiology and Biotechnology
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    • v.13 no.4
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    • pp.501-508
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    • 2003
  • Artificial coupling of one enzyme with another can provide an efficient means for the production of industrially important chemicals. Xylose reductase has been recently discovered to be useful in the reductive production of xylitol. However, a limitation of its in vitro or in vivo use is the regeneration of the cofactor NAD(P)H in the enzyme activity. In the present study, an efficient process for the production of xylitol from D-xylose was established by coupling two enzymes. A NADH-dependent xylose reductase (XR) from Pichia stipitis catalyzed the reduction of xylose with a stoichiometric consumption of NADH, and the resulting cofactor $NAD^+$ was continuously re-reduced by formate dehydrogenase (FDH) for regeneration. Using simple kinetic analyses as tools for process optimization, suitable conditions for the performance and yield of the coupled reaction were established. The optimal reaction temperature and pH were determined to be about $30^{\circ}C$ and 7.0, respectively. Formate, as a substrate of FDH, affected the yield and cofactor regeneration, and was, therefore, adjusted to a concentration of 20 mM. When the total activity of FDH was about 1.8-fold higher than that of XR, the performance was better than that by any other activity ratios. As expected, there were no distinct differences in the conversion yields of reactions, when supplied with the oxidized form $NAD^+$ instead of the reduced form NADH, as a starting cofactor for regeneration. Under these conditions, a complete conversion (>99%) could be readily obtained from a small-scale batch reaction.

Hansenula sp. MS-364의 생육과 Formate Dehydrogenase의 활성

  • 유병욱;권태종
    • Microbiology and Biotechnology Letters
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    • v.25 no.4
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    • pp.403-407
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    • 1997
  • Medium components for maximum activity of NAD$^{+}$-dependent formate dehydrogenase (EC 1.2.1.2; FDH) were optimized with a methanol-assimilating yeast Hansenula sp. MS-364, preserved by our laboratory. The maximum activity of the enzyme was obtained when the strain was cultivated at 30$circ$C for 24 hours in a medium containing methanol 3%(v/v), yeast extract 0.8%(w/v), K$_{2}$HPO$_{4}$, 0.1%(w/v), KH$_{2}$PO$_{4}$ 0.1%(W/V), MgSO$_{4}$, 7H$_{2}$O 0.05%(w/v), and the pH of the culture broth was adjusted at 5.0.

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