• Journal of Food Hygiene and Safety
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• v.23 no.4
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• pp.324-329
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• 2008

An analytical method for the simultaneous determination of nine quinolones (QNs) namely, marbofloxacin, norfloxacin(IS), ciprofloxacin, danofloxacin, enrofloxacin, sarafloxacin, difloxacin, oxolinic acid, and flumequine in flatfish and egg was developed and validated using liquid chromatography with fluorescence detection (LC-FD). The samples were extracted using a traditional liquid-liquid extraction process; deproteinization was accomplished by the addition of trichloroacetic acid and acetonitrile (ACN), and defatting was performed with hexane. Chromatographic separation was achieved on a reverse phase C8 column with gradient elution using a mobile phase of 200 mM ammonium acetate buffer (pH 4.5) and ACN. The proposed method was validated according to the CODEX guideline. Mean recoveries of QNs from flatfish and egg were 89.6-106.5% with relative standard deviations (RSDs) below 15% at three different concentrations of 50, 100 and $500{\mu}g/kg$. Linearity was obtained with a correlation coefficient ($r^2$) of 0.9989-1.0000. The LOD for the investigated QNs was $1-16{\mu}g/kg$ depending on flatfish and egg. The present method can be applied simultaneously to determine QNs in muscle of flatfish and egg.

• The Journal of Internal Korean Medicine
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• v.42 no.3
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• pp.259-278
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• 2021

Objectives: This study examines the effect of Sagunja-tang on functional dyspepsia (FD) through a systematic review and meta-analysis of a randomized controlled trial (RCT). Methods: A search for RCTs that tested the effect of Sagunja-tang on functional dyspepsia was conducted in Medline, Embase, PubMed, CENTRAL, CiNii, CNKI, NDSL, RISS, OASIS, and KISS databases on November 8, 2020, with no limit on the year of publication. A meta-analysis was performed by synthesizing the findings, including total efficiency, clinical symptom score, myosin light-chain kinase (MLCK) level (pg/mL, and gastric half-emptying time (min). RevMan 5.4.1 software was used for data analysis. The quality of the literature was evaluated using Cochrane's risk of bias (RoB) tool. Results: A total of 14 RCTs met the selection criteria. As a result of the meta-analysis, the treatment group had higher total efficacy and MLCK levels (gastric antrum, jejunum) than the control group, and the clinical symptom score and gastric half-emptying time were lower. However, due to the low quality of the included RCT and the small sample size, the results may be slightly biased.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.6
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• pp.880-884
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• 2001

Rumen microbiology research has undergone several evolutionary steps: the isolation and nutritional characterization of readily cultivated microbes; followed by the cloning and sequence analysis of individual genes relevant to key digestive processes; through to the use of small subunit ribosomal RNA (SSU rRNA) sequences for a cultivation-independent examination of microbial diversity. Our knowledge of rumen microbiology has expanded as a result, but the translation of this information into productive alterations of ruminal function has been rather limited. For instance, the cloning and characterization of cellulase genes in Escherichia coli has yielded some valuable information about this complex enzyme system in ruminal bacteria. SSU rRNA analyses have also confirmed that a considerable amount of the microbial diversity in the rumen is not represented in existing culture collections. However, we still have little idea of whether the key, and potentially rate-limiting, gene products and (or) microbial interactions have been identified. Technologies allowing high throughput nucleotide and protein sequence analysis have led to the emergence of two new fields of investigation, genomics and proteomics. Both disciplines can be further subdivided into functional and comparative lines of investigation. The massive accumulation of microbial DNA and protein sequence data, including complete genome sequences, is revolutionizing the way we examine microbial physiology and diversity. We describe here some examples of our use of genomics- and proteomics-based methods, to analyze the cellulase system of Ruminococcus flavefaciens FD-1 and explore the genome of Ruminococcus albus 8. At Illinois, we are using bacterial artificial chromosome (BAC) vectors to create libraries containing large (>75 kbases), contiguous segments of DNA from R. flavefaciens FD-1. Considering that every bacterium is not a candidate for whole genome sequencing, BAC libraries offer an attractive, alternative method to perform physical and functional analyses of a bacterium's genome. Our first plan is to use these BAC clones to determine whether or not cellulases and accessory genes in R. flavefaciens exist in clusters of orthologous genes (COGs). Proteomics is also being used to complement the BAC library/DNA sequencing approach. Proteins differentially expressed in response to carbon source are being identified by 2-D SDS-PAGE, followed by in-gel-digests and peptide mass mapping by MALDI-TOF Mass Spectrometry, as well as peptide sequencing by Edman degradation. At Ohio State, we have used a combination of functional proteomics, mutational analysis and differential display RT-PCR to obtain evidence suggesting that in addition to a cellulosome-like mechanism, R. albus 8 possesses other mechanisms for adhesion to plant surfaces. Genome walking on either side of these differentially expressed transcripts has also resulted in two interesting observations: i) a relatively large number of genes with no matches in the current databases and; ii) the identification of genes with a high level of sequence identity to those identified, until now, in the archaebacteria. Genomics and proteomics will also accelerate our understanding of microbial interactions, and allow a greater degree of in situ analyses in the future. The challenge is to utilize genomics and proteomics to improve our fundamental understanding of microbial physiology, diversity and ecology, and overcome constraints to ruminal function.

• Journal of the Society of Disaster Information
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• v.17 no.1
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• pp.120-127
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• 2021

Purpose: In this study, the effect of changes in the variables related to water droplets on the spray density on the floor in the analysis of the water mist fire protection system using FDS was analyzed. Method: When the spray of the water mist nozzle was analyzed in FDS, Particles Per Seconds, Particle Velocity, Size Distribution, and Spray Pattern Shape that can be set in relation to droplets were input to review the analyzed results. Result: In the analysis results, when the number of particles per second was set above a certain value, the spray density of the floor was similar. In the case of Particle Velocity, as the velocity decreases, the spray density of the central portion increases but decreases at a distance of 0.15m or more. From the analysis of the change in the size distribution function, it was found that an increase in the 𝛾 value increases the spray density of the central part, but the value at a remote location decreases. Compared to the result of applying the Gaussian distribution, the median value decreases dramatically when the uniform distribution is applied, but the value at the adjacent position increases. Conclusion: Variables related to the droplet properties of the FDS affect the spray density of the floor. Therefore, in order to increase the reliability of results before performing analyses such as fire suppression or cooling, a sufficient review of input variables is required.

• Journal of Environmental Science International
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• v.25 no.8
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• pp.1065-1076
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• 2016

This study analyzes the $PM_{10}$ characteristics (particulate matter with aerodynamic diameter less than $10{\mu}m$), concentration, and emissions in eight large South Korean cities (Seoul, Incheon, Daejeon, Daegu, Gwangju, Ulsan, Busan, Jeju). The annual median of $PM_{10}$ concentration showed a decline of $0.02{\sim}1.97{\mu}g/m^3$ in the regions, except for Incheon, which recorded an annual $0.02{\mu}g/m^3$ increase. The monthly distribution levels were high in spring, winter, fall, and the summer, but were lower in summer for all regions except for Ulsan. These differences are thought to be due to the dust in spring and the cleaning effect of precipitation in summer. The variation in concentrations during the day (diurnal variation) showed that $PM_{10}$ levels were very high during the rush hour and that this was most extreme in the cities (10.00 and 18.00-21.00). The total annual $PM_{10}$ emissions analysis suggested that there had been a general decrease, except for Jeju. On-road mobile (OM) sources, which contributed a large proportion of the particulates in most regions, decreased, but fugitive dust (FD) sources increased in the remaining regions, except for Daegu. The correlation analysis between $PM_{10}$ concentrations and emissions showed that FD could be used as a valid, positive predictor of $PM_{10}$ emissions in Seoul (74.5% (p<0.05)), Dajeon (47.2% (p<0.05)), and Busan (59.1% (p<0.01)). Furthermore, industrial combustion (IC) was also a significant predictor in Incheon (61.7% (p<0.01)), and on-road mobile (OC) sources were a valid predictor in Daegu (24.8% (p<0.05)).

• Journal of the Korea Institute of Building Construction
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• v.13 no.6
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• pp.530-540
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• 2013

The business environment that affects the management performance can be characterized by each Strategic Business Unit (SBU) since construction companies win overseas contracts due to the fairly good construction situations while experience a decline in the local housing market. Environmental changes can alter the strategic importance of the SBU when measuring the management performance. However, large construction companies apply BSC (Balanced Score Card) for collective calculation to determine the management performance, making it difficult to reflect the strategic importance of SBU. This method may create a distorted image of management performance that fails to take environmental changes into consideration, and as such it needs to be improved. Yet, there are no studies on the weight of each SBU considering environmental changes. Thus, the current study intends to analyze the weight of SBU for company-wide measurement of the performance of large construction companies. In addition, a model for analysis of SBU importance is proposed to respond to the constantly changing environmental situations and to modify the weight. For analysis of SBU weight, a questionnaire was conducted with 23 experts and hands-on workers, and the questionnaire result was quantitatively analyzed by applying the FD-AHP method. It is expected that the result will enable a model to be proposed to calculate the weight per division in a manner that reflects environmental changes and minimizes strategic distortion when measuring the management performance of large construction companies.

• Korean Journal of Ecology and Environment
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• v.54 no.4
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• pp.384-389
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• 2021

This study was conducted on October 23, 2020 at the Cheongpyeong Reservoir located in Seorakmyeon, Gapyeong-gun, Gyeonggi-do, and analyzed the horizontal and vertical distribution patterns of fish based on data obtained using fishfinder. The total surface area of fishfinder survey conducted was 782,853 m², and where the water depth (WD) ranges from 10 m to 12 m is widest which 31.7% of total surface area. As a result of the heat map analysis, fish density was highest at right bank under the Gapyeong-bridge, but there was no specific pattern in horizontal distribution of fish. As a result of vertical distribution of fish analysis, 86.6% of fishes are observed at below 6 m of the fish depth (FD, distance from water surface to fish). As a result of the relative height (RH, water depth-distance from bottom to fish ratio) analysis, there was a tendency that fishes are distributed in near surface area more as the WD increased. This tendency could have various reasons such as water temperature gradient along the water depth, and further studies are required for detailed explanation.

• Fire Science and Engineering
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• v.22 no.3
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• pp.287-292
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• 2008

In this study, the evacuation training were performed in a high school building in Cheong-Ju and compared with the simulation program (Simulex). Also numerical analysis of room fire in school building was conducted by fire model (FDS, CFAST) and evaluated the available safe egress time for the safety assessment. As a result, the 8% of total egress persons were failed to escape at evacuation training and 40% of total egress persons were failed to escape at Simutex simulation. Simutex program was not reflected the real escape conditions, such as evacuation route, refuge place, etc.

• 제어로봇시스템학회:학술대회논문집
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• 2004.08a
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• pp.480-483
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• 2004

This paper, we present the evaluation of outage probability on the SNR under the overlapped carrier allocation scheme in the reverse link with imperfect power control of a cellular CDMA system, which is base on using Gaussian approximation. In numerical results, the band limited pulse generated by square-root of raised consin pulse shaping filters (SRRC) of the transmitted signal is investigated and compared in conventional FD/CDMA and OC-CDMA of the outage probability. It will be also show that the outage probability can be improved by overlapping of carriers. We use error statistics to model the intra-cell interference and evaluate the impact of different mobiles velocity and number of resolvable paths on the system performance.

• Journal of the Korean Institute of Telematics and Electronics B
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• v.33B no.3
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• pp.176-185
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• 1996

Transformin the huge size of drawings into a suitable format for CAD system and recognizng the contents of drawings are the major concerans in the automated analysis of engineering drawings. This paper proposes some methods for text/graphics separation, symbol extraction, vectorization and symbol recognition with the object of applying them to electronic cirucit drawings. We use MBR (Minimum bounding rectangle) and size of isolated region on the drawings for separating text and graphic regions. Characteristics parameters such as the number of pixels, the length of circular constant and the degree of round shape are used for extracting loop symbols and geometric structures for non-loop symbols. To recognize symbols, nearest netighbor between FD (foruier descriptor) of extractd symbols and these of classification reference symbols is used. Experimental results show that the proposed method can generate compact vector representation of extracted symbols and perform the scale change and rotation of extracted symbol using symbol vectorization. Also we achieve an efficient searching of circuit drawings.