• The Korean Journal of Physiology and Pharmacology
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• 제13권6호
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• pp.483-489
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• 2009

Despite the potential importance of the human regulator of calcineurin 1 (RCAN-1) gene in the modulation of cell survival under stress, little is known about its role in death-inducing signal pathways. In this study, we addressed the effects of RCAN1.4 knockdown on cellular susceptibility to apoptosis and the activation of death pathway proteins. Transfection of siRNAs against RCAN1.4 resulted in enhanced Fas- and etoposide-induced apoptosis, which was associated with increased expression and translocation of Bax to mitochondria. Our results suggest that enhanced expression and activation of p53 was responsible for the upregulation of Bax and the increased sensitivity to apoptosis, which could be reversed by p53 knockdown. To explain the observed upregulation of p53, we propose a downregulation of the ubiquitin ligase HDM2, probably translationally. These findings show the importance of appropriate RCAN1.4 expression in the modulation of cell survival and reveal a link between RCAN1.4 and p53.

• Nutrition Research and Practice
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• 제18권2호
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• pp.180-193
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• 2024

BACKGROUND/OBJECTIVES: Obesity is a major cause of metabolic disorders; to prevent obesity, research is ongoing to develop natural and safe ingredients with few adverse effects. In this study, we determined the anti-obesity effects of Rosa multiflora root extract (KWFD-H01) in 3T3-L1 adipocytes and Sprague-Dawley (SD) rats. MATERIALS/METHODS: The anti-obesity effects of KWFD-H01in 3T3-L1 adipocytes and SD rats were examined using various assays, including Oil Red O staining, gene expression analyses, protein expression analyses, and blood biochemical analyses. RESULTS: KWFD-H01 reduced intracellular lipid accumulation and inhibited the mRNA expression of peroxisome proliferator-activated receptor γ (PPARγ), cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT)/enhancer binding proteins (C/EBPα), sterol regulatory element-binding transcription factor 1 (SREBP-1c), acetyl-CoA carboxylase (ACC), and fatty acid synthase (FAS) in 3T3-L1 cells. KWFD-H01 also reduced body weight, weight gain, and the levels of triglycerides, total and LDL-cholesterol, glucose, and leptin, while increasing high-density lipoprotein-cholesterol and adiponectin in SD rats. PPARγ, C/EBPα, SREBP-1c, ACC, and FAS protein expression was inhibited in the epididymal fat of SD rats. CONCLUSION: Overall, these results confirm the anti-obesity effects of KWFD-H01 in 3T3-L1 adipocytes and SD rats, indicating their potential as baseline data for developing functional health foods or pharmaceuticals to control obesity.

• 생명과학회지
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• 제12권6호
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• pp.769-779
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• 2002

식생활의 변화로 인해 fast food 및 가공식품의 소비가 급속히 증가하고 있으며 이러한 식품에 많이 함유되어 있는 trans 지방산의 생리적 기능에 대한 관심이 모아지고 있다. 따라서 본 연구에서는 한국인이 즐겨 섭취하는 유지중 trans 지방산 함량을 함유하고 있는 마가린, 버터의 장기간 섭취가 횐쥐의 콜레스테롤 대사와 세포막 유동성에 미치는 영향을 옥수수유와 비교하여 trans 지방산 함유 식품의 생리기능성을 제시하고자 하였다. 이유 직후의 흰쥐(S.D. male)를 trans 지방산 함량이 다른 마가린(25%)과 버터(8.5%), 옥수수유를 포함하는 식이로 각각 8주간 섭취시켜 사육한 결과, 체중증가와 1일 식이 섭취량은 식이군간에 차이가 없었으나 trans 지방산을 함유한 식이군(마가린, 버터)의 식이효율이 감소하였다. 혈장 지질 조성에서 중성지방 농도는 차이가 없었지만, 콜레스테롤 농도는 옥수수유 식이군에 비해 trans 지방산을 함유한 식이군에서 유의적으로 낮았다. 지단백 획분 중 LDL 콜레스테롤 농도는 옥수수유 식이군에 비해 마가린 식이군에서 유의적으로 낮았으며, 버터 식이군에서 유의적으로 높았다. HDL 콜레스테롤 농도는 버터 식이군이 다른 식이군에 비해 낮았으며 그 결과 버터 식이군의 동맥경화지수가 가장 높았다. 또한 간 중성지방 함량은 마가린과 옥수수유 식이군에 비해 버터 식이군에서 낮았으며 콜레스테롤의 농도는 옥수수유 식이군에서 가장 높았다. 간 조직으로의 trans 지방산의 축적 정도는 마가린 식이군에서 가장 높았으며 간의 HMG-CoA reductase 활성은 버터와 마가린 식이군에서 다소 감소하였다. 분변으로의 콜레스테롤 배설량은 trans 지방산을 함유한 마가린과 버터 식이군에서 유의적으로 높았으며 배설되는 trans 지방산 함량은 마가린 식이군에서 가장 높았다. 세포막 유동성은 옥수수유군에 비해 마가린과 버터식이군에서 유의적으로 감소하였다. 본 연구의 결과로 미루어 볼 때 trans 지방산 함유 지방 섭취에 의한 고콜레스테롤혈증 유발효과는 확인할 수 없었으며, trans 지방산의 섭취가 분변으로의 콜레스테롤 배설을 증가시키고 HMG-CoA reductase 활성을 저하시켜 혈장 콜레스테롤을 감소시킨 것으로 사료되며 버터군에서 동맥경화지수가 가장 높게 나타난 것은 버터의 포화지방산, 특히 미리스틴산과 팔미트산의 높은 함량 때문으로 사료된다. 그러나 마가린과 버터식이군의 세포막 유동성이 현저히 감소한 것은 세포막에서 trans 지방산이 포화지방산과 유사한 거동을 보이는 때문으로 풀이된다.

• Clinical and Experimental Reproductive Medicine
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• 제46권4호
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• pp.152-165
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• 2019

Objective: This study aimed to examine the effect of vitrification on apoptosis and survival in human preantral follicles after thawing. Methods: This experimental study was conducted at an acute tertiary care hospital from March 2012 to April 2013. Ovaries were sliced into 5 × 5 × 1-mm pieces and divided into the following three groups: preantral follicle isolation, ovarian tissue vitrification-warming followed by follicle isolation, and immunohistochemistry of fresh ovarian tissue. For statistical analyses, the Student t-test, chi-square test, Kruskal-Wallis test, and Kaplan-Meier survival analysis were used. Results: A total of 161 preantral follicles (70% secondary) were collected from ovarian cortex tissue of six women between 30 and 37 years of age who underwent oophorectomy due to cervical cancer or breast cancer. There were no significant differences in the follicular morphology of fresh preantral follicles and vitrified follicles after thawing. The mean Fas ligand (FasL) mRNA expression level was 0.43 ± 0.20 (relative to β-actin) in fresh preantral follicles versus 0.51 ± 0.20 in vitrified follicles (p= 0.22). The mean caspase-3 mRNA expression level in fresh preantral follicles was 0.56 ± 0.49 vs. 0.27 ± 0.21 in vitrified follicles (p= 0.233). One vitrified-thawed secondary follicle grew and developed to an antral follicle within 6 days of culture. Conclusion: Vitrification did not affect preantral follicle morphology or mRNA expression of the apoptosis markers FasL and caspase-3. Further studies are required to establish whether vitrification affects the outcomes of in vitro culture and the maturation of preantral follicles.

• 한국음향학회지
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• 제40권6호
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• pp.587-592
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• 2021

노화 및 골다공증으로 인해 긴 뼈에서 발생하는 피질골의 두께 변화는 골절의 위험인자로 알려져 있다. 본 연구는 200 kHz의 중심주파수 및 12.7 mm의 직경을 갖는 한 쌍의 트랜스듀서와 함께 축방향 전파법을 이용하여 윗면이 2 mm 두께의 실리콘 고무층으로 덮인(1 mm부터 4 mm까지의 두께를 갖는) 아크릴판으로 제작된 7개의 연조직-골 모사 팬텀에서 피질골 두께에 대한 유도초음파 위상속도의 의존성을 고찰하였다. 모든 연조직-골 모사 팬텀에서 서로 다른 속도를 갖는 First Arriving Signal(FAS) 및 Slow Guided Wave(SGW)가 전파하는 것으로 일관되게 관찰되었다. FAS의 위상속도는 피질골 두께가 증가함에 따라 약간 감소하는 반면 SGW의 위상속도는 피질골 두께가 증가함에 따라 크게 감소하는 것으로 나타났다. FAS 및 SGW의 위상속도는 각각 실리콘 고무층을 갖지 않는 아크릴판에서 전파하는 S0 및 A0 램 모드의 위상속도와 거의 일치하는 것으로 나타났으며, 이는 아크릴판의 윗면을 덮고 있는 연조직 모사 물질(2 mm 두께의 실리콘 고무층)의 존재가 위상속도 측정에 큰 영향을 미치지 않는다는 것을 의미한다.

• 한국결정성장학회지
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• 제19권6호
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• pp.277-281
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• 2009

초발수 $SiO_2$ 박막을 sol-gel법에 의해 유리 기판 위에 성공적으로 제조하였다. 높은 표면 조도를 갖는 $SiO_2$ 박막을 제조하기 위하여 tetraethoxysilane(TEOS) 용액에 $SiO_2$ 나노 입자들을 첨가하였다. $iO_2$ 입자를 첨가하지 않은 용액을 이용하여 제조한 코팅막은 RMS roughness가 1.27 nm의 매우 평평한 표면 구조를 나타낸 반면, $SiO_2$ 나노 입자들을 1.0, 2.0, 3.0 wt% 첨가한 용액을 이용하여 제조한 $SiO_2$ 박막의 RMS roughness는 44.10 nm, 69.58 nm, 80.66 nm로 측정되었다. 제조된 $SiO_2$ 박막의 표면을 소수성 표면으로 바꾸기 위하여 FAS 용액을 이용하여 발수 처리를 하였다. FAS 처리 이후 거친 표면구조를 갖는 $SiO_2$ 박막의 표면은 친수성에서 소수성으로 바뀌었고 특히, 80.66 nm의 RMS roughness를 갖는 박막은 $163^{\circ}$의 물 접촉각을 갖는 초발수 표면을 나타내었다.

• Journal of Life Science
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• 제12권2호
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• pp.57-60
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• 2002

Apoptosis of vascular smooth muscle cell is observed in the vascular diseases such as atherosclerosis and restenosis. The death of vascular smooth muscle cells can be induced by cytokines and activation of Fas-pathways. It is widely accepted that apoptosis occurs without inflammation. There are, however, reports that apoptosis is not silent. Vascular smooth muscle cells dying by Fas-pathway secreted inflammatory cytokines including monocyte chemoattractant protein-1. This study have investigated whether apoptosis is associated with potent inflammatory cytokine tumor tumor necrosis factor (TNF)-${\alpha}$. The cells which undergo apoptosis by expressing FADD in the absence of tetracycline expressed and secreted TNF-${\alpha}$. When the level of TNF-${\alpha}$ transcript was investigated, dying smooth muscle cells exhibited transcriptional activation of TNF-${\alpha}$. The data indicate that dying vascular smooth muscle cells contribute to inflammation by expressing inflammatory cytokines. The present study suggests that apoptosis could not be silent in certain pathological situations.

• International Journal of Fuzzy Logic and Intelligent Systems
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• 제9권3호
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• pp.161-165
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• 2009

Increase of multimedia information in Web requires a new method to manage and service multimedia documents efficiently. This paper proposes a conceptual object grouping method by fuzzy filtering, which is automatically constituted based on increase of multimedia documents. The proposed method composes subsumption relations between conceptual objects automatically using fuzzy filtering of the document objects that are extracted from domains. Grouping of such conceptual objects is regarded as subsumption relation which is decided by $\mu$-cut. This paper proposes $\mu$-cut, FAS(Fuzzy Average Similarity) and DSR(Direct Subsumption Relation) to decide fuzzy filtering, which groups related document objects easily. This paper used about 1,000 conceptual objects in the performance test of the proposed method. The simulation result showed that the proposed method had better retrieval performance than those for OGM(Optimistic Genealogy Method) and BGM(Balanced Genealogy Method).

• 한국치위생학회지
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• 제17권6호
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• pp.1207-1214
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• 2017

Objectives: This study aims to investigate degree of scaling fear (Fear when scaling: FWS, Fear from dental hygienist unbelief: FFDHU, and Fear after scaling: FAS) in college students. Methods: 113 students were recruited for the study in Daegu Health College between March and June of 2017. Frequency table of general characteristics was generated, and then the t-test and ANOVA (scheffes's post hoct) were used to analyze the differences between scaling fear and two or three groups of variables. Results: Mean scores of scaling fear in 113 students were 2.24 (FWS), 1.76 (FFDHU), and 1.76 (FAS). Score 2.48 of female's scaling fear (FWS) was significantly higher than men's 2.02 (p<0.05). Smokers who have smoked less than 3 years (2.56) (FWS) were lower than those who have smoked over 3 years (1.55) (p<0.01). Score of students want the explanation of scaling when scaling (1.94) (FWS) were significantly higher than those who does not (1.59) (p<0.05). Conclusions: The findings of this study showed that there were gender and smoking periods when scaling to effect a score of scaling fear.

• Asian Pacific Journal of Cancer Prevention
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• 제13권1호
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• pp.81-85
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• 2012

Purpose: To study enhancing effects of paclitaxel in the thermochemotherapy of osteosarcoma cell lines and related mechanisms. Materials and Methods: Paclitaxel and carboplatin were used alone or jointly on OS732 cell lines in the presence of hyperthermia. Inhibition of proliferation was measured by MTT assay and cellular changes were assessed with inverted phase contrast and fluorescence microscopy. Apoptosis was analyzed with flow cytometry (FCM) and Fas expression by immunocytochemistry. Results: At $43^{\circ}C$, one hour after the application of 10ug/ml paclitaxel and $5{\mu}g/ml$ carboplatin on OS732 cells jointly, the survival rate was 15.8% which was significantly lower than with $10{\mu}g/ml$ paclitaxel (45.8%) and $5{\mu}g/ml$ carboplatin (47.7%) respectively (P<0.01). Moreover, changes of morphology and apoptotic rates indicated that the apoptosis-inducing effect of combined application was also much enhanced, as evident also regarding Fas expression. Conclusion: Paclitaxel is conducive to thermochemotherapy of osteosarcoma cell lines, possibly accomplished by up-regulation of Fas expression with induction of apoptosis.