• IMMUNE NETWORK
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• v.9 no.6
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• pp.243-247
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• 2009

In this study, we report the development of a new dual reporter vector system for the analysis of promoter activity. This system employs green fluorescence emitting protein, EGFP, as a reporter, and uses red fluorescence emitting protein, DsRed, as a transfection control in a single vector. The expression of those two proteins can be readily detected via flow cytometry in a single analysis, with no need for any further manipulation after transfection. As this system allows for the simultaneous detection of both the control and reporter proteins in the same cells, only transfected cells which express the control protein, DsRed, can be subjected to promoter activity analysis, via the gating out of all un-transfected cells. This results in a dramatic increase in the promoter activity detection sensitivity. This novel reporter vector system should prove to be a simple and efficient method for the analysis of promoter activity.

• Development and Reproduction
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• v.15 no.1
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• pp.41-52
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• 2011

In the present study, embryoid bodies (EBs) obtained from induced pluripotent stem cells (iPSCs) were induced to differentiate into germ lineage cells by treatment with bone morphogenetic protein 4 (BMP4) and retinoic acid (RA). The results were compared to the results for embryonic stem cells (ESCs) and multipotent spermatogonial stem cells (mSSCs) and quantified using immunocytochemical analysis of germ cell-specific markers (integrin-${\alpha}6$, GFR-${\alpha}1$, CD90/Thy1), fluorescence activating cell sorting (FACS), and real time-RT-PCR. We show that the highest levels of germ cell marker-expressing cells were obtained from groups treated with 10 ng/$m{\ell}$ BMP4 or 0.01 ${\mu}M$ RA. In the BMP4-treated group, GFR-${\alpha}1$ and CD90/Thy-1 were highly expressed in the EBs of iPSCs and ESCs compared to EBs of mSSCs. The expression of Nanog was much lower in iPSCs compared to ESCs and mSSCs. In the RA treated group, the level of GFR-${\alpha}1$ and CD90/Thy-1 expression in the EBs of mSSCs Induced pluripotent stem cells, Mouse embryonic stem cells, Multipotent spermatogonial stem cells, Germ cell lineage, Differentiation potential. was much higher than the levels found in the EBs of iPSCs and similar to the levels found in the EBs of ESCs. FACS analysis using integrin-${\alpha}6$, GFR-${\alpha}1$, CD90/Thy1 and immunocytochemistry using GFR-${\alpha}1$ antibody showed similar gene expression results. Therefore our results show that iPSC has the potential to differentiate into germ cells and suggest that a protocol optimizing germ cell induction from iPSC should be developed because of their potential usefulness in clinical applications requiring patient-specific cells.

• Biomedical Science Letters
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• v.18 no.3
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• pp.210-217
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• 2012

Oncolytic viral vectors have shown good candidates for cancer treatment but have many limitations. To improve the therapeutic potential of oncolytic vaccinia virus, we developed a recombinant vaccinia virus expressing the 4-1BBL co-stimulatory molecule or CCL21. 4-1BBL and CCL21 expression was identified by FACS analysis and immunoblotting. rV-4-1BBL vaccination shows significant tumor regression compared to rV-LacZ, but rV-CCL21 shows rapid tumor growth compared to rV-LacZ in the poorly immunogenic B16 murine melanoma model. 4-1BBL expression resulted in the increase of the number of CD8+ T cells and especially the increase of effector (CD62L-CD44+) CD8+ T cells. These data suggest 4-1BBL may be the potential target for enhancement of tumor immunotherapy.

• Proceedings of the Korean Society of Computer Information Conference
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• 2009.01a
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• pp.163-167
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• 2009

본 논문에서는 크게 2단계에 걸쳐 다양한 형태의 얼굴을 가진 2차원 애니메이션 상의 캐릭터 얼굴구성요소를 추출하고 표정을 분석한다. 첫 번째 단계에서는, 기존의 얼굴인식 및 표정인식 분야에서 이용되었던 동적메쉬모델을 간소화하여 캐릭터 얼굴에 적용하기 위한 최적의 표준 메쉬모델을 제작하고, 이 모델을 사용하여 얼굴구성요소의 위치 및 형태정보를 추출한다. 두 번째 단계에서는, 앞 단계에서 추출된 3가지 얼굴구성요소(눈썹, 눈, 입)를 사용하여 FACS(Facial Action Coding System)에 정의된 AU(Action Units) 44개 중 12개의 AU를 사용하여 캐릭터의 5까지 기본적인 얼굴 표정에 대해 분석 및 정의한다. 본 논문에서 정의한 AU로 기본적인 5가지 얼굴표정에 대해 표정분석 정확도를 측정하였고, 서로 다른 캐릭터에 실험함으로써 제안된 AU정의의 타당성을 제시한다.

• Journal of Microbiology and Biotechnology
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• v.16 no.7
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• pp.1149-1153
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• 2006

In this study, nuclease-resistant RNA aptamers that are specific for Jurkat T leukemia cells were selected by a subtractive systemic evolution of ligands by exponential enrichment (SELEX) method. A randomized nuclease-resistant RNA library was incubated with normal peripheral blood mononuclear cells (PBMC) in each round to preclude RNAs that recognize the common cellular components on the surface of normal and cancer cells. The precluded RNAs were used for the selection of Jurkat T cell-specific aptamers, and the specific RNAs were then gradually enriched from start to the following selections. After 16 rounds of the subtractive SELEX, the selected aptamers were found to preferentially bind to Jurkat T cells, but not to the normal PBMC, evidenced by fluorescence-activated cell sorting analysis. Thus, the subtractive SELEX can be used to identify ligands to cancer-specific biological markers without prior knowledge of the nature of markers. The aptamers could be applied to specific cell sorting, tumor therapy, and diagnosis, and moreover, to find cancer cell-specific markers.

• The Journal of Internal Korean Medicine
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• v.19 no.2
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• pp.125-138
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• 1998

To evaluate the radioprotective effects of Bujeongsaengjintang studies were done experimentally. The results were obtained as follows: 1. WBC, Platelet and RBC were significantly increased in Bujeongsaengjintang treated group as compared with control group after exposure to radiation by Liniac. 2. By FACS analysis of splenic leukocyte after exposure to radiation by Liniac, T cell, T-helper cell and macrophase were significantly increased in Bujeongsaengjintang treated group. 3. In histological changes of ileum and jejunum of Balb/C mice after exposure to radiation by Liniac, exclusion and fusion of villi were decreased in Bujeongsaengjintang treated group as compared with control group. From above results, it is suggested that Bujeongsaengjintang is available to a clinic for the protection from damage by radiotherapy to cancer.

• Biomolecules & Therapeutics
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• v.8 no.1
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• pp.22-26
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• 2000

In order to study the cytotoxic properties of sesquitepenes, dehydrocostus lactone (DL) and costunolide from Saussurea lappa, cytotoxicity was measured by SRB method using various human cancer cell lines. Dehydrocostus lactone(DL) and costunolide exhibited significant cytotoxicity against A-549, SK-OV-3, SK-MEL-2, XF-498 and HCT 15 cells. The U937 human leukemia cells treated with DL showed several apoptotic evidences like chromosome condensation and formation of apoptotic bodies. From the results of FACS analysis, early apoptosis was observed by phosphatidylserine externalization detected by annexin V-FITC. Furethermore, these studies determined hypodiploid contents and effects on the cell phase distribution of DL-treated U937 cells. After exposure of U937 cells to $30\mu\textrm{M}$ DL effectively led to G2/M modified cell cycle distribution within 24hr. These observations suggest that DL can be used efficiently for the cancer treatment.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2008.04a
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• pp.435-438
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• 2008

본 논문에서는 애니메이션을 위해서 얼굴의 특징표현(Action Units)의 조합하는 방법으로 얼굴 모델링을 하기 위한 3D대응점(3D dense correspondence)을 찾는 방법을 제시한다. AUs는 표정, 감정, 발음을 나타내는 얼굴의 특징표현으로 통계적 방법인 PCA (Principle Component Analysis)를 이용하여 만들 수 있다. 이를 위해서는 우선 3D 모델상의 대응점을 찾는 것이 필수이다. 2D에서 얼굴의 주요 특징 점은 다양한 알고리즘을 이용하여 찾을 수 있지만 그것만으로 3D상의 얼굴 모델을 표현하기에는 적합하지 않다. 본 논문에서는 3D 얼굴 모델의 대응점을 찾기 위해 원기둥 좌표계 (Cylinderical Coordinates System)을 이용하여 3D 모델을 2D로 투사(Projection)시켜서 만든 2D 이미지간의 워핑(Warping) 을 통한 대응점을 찾아 역으로 3D 모델간의 대응점을 찾는다. 이것은 3D 모델 자체를 변환하는 것보다 적은 연산량으로 계산할 수 있고 본래 형상의 변형이 없다는 장점을 가지고 있다.

• Journal of Haehwa Medicine
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• v.9 no.2
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• pp.123-134
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• 2001

In order to investigate the effect of Mori Folium on immunologic control function, the various immune responses were studied. The results were obtained as follows : 1. The transformation of lymphocyte was increased significantly in all concentration of Mori Folium treated group as compared with negative and positive control. 2. In FACS analysis, the population of helper T cell and T cell were increased significantly in Mori Folium treated group as compared with control. 3. IL-12(p35) and INF-${\gamma}$ were upregulated in Mori Folium treated group 4. NO and carbon clearance were significantly increased in Mori Folium treated group as compared with control. 5. The hemagglutinin titer and hemolysin titer were increased but insignificantly in Mori Folium treated group as compared with control.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.3
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• pp.1-12
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• 2006

Purpose : This study was aimed to investigate the inhibitory effect of Leonurus sibiricus on the proliferation of human uterine leiomyoma cells and the expression of gene related the mechanism of cell apoptosis. Methods : We counted the number of death cells treated with indicated concentration of Leonurus sibiricus and investigated cell death rate by MTS assay. Furthermore, flow cytometry analysis and DNA fragmentation assay were used to dissect between necrosis and apoptosis and then we observed the differential gene expression by western blot analysis. Results : Leonurus sibiricus significantly inhibited the proliferation of uterine leiomyoma cell in a dose-dependent and time dependent manner. Fluorescence activated cell sorter (FACS) analysis indicated that Leonurus sibiricus induced G1 cell cycle arrest. Leonurus sibiricus enhanced the expression of p27 and p53 with cell cycle arrest. Conclusion : These findings suggest that Leonurus sibiricus is a candidate agent for the treatment of uterine leiomyoma. p27, $p53^{1}$ may play an important role in Leonurus sibiricus-induced cell cycle arrest and cell growth inhibition.