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Oncolytic Vaccinia Virus Expressing 4-1BBL Inhibits Tumor Growth by Increasing CD8+ T Cells in B16F10 Tumor Model  

Lee, Na-Kyung (Department of Biomedical Laboratory Science, Soon Chun Hyang University)
Kim, Hong-Sung (Department of Biomedical Laboratory Science, Korea Nazarene University)
Publication Information
Biomedical Science Letters / v.18, no.3, 2012 , pp. 210-217 More about this Journal
Abstract
Oncolytic viral vectors have shown good candidates for cancer treatment but have many limitations. To improve the therapeutic potential of oncolytic vaccinia virus, we developed a recombinant vaccinia virus expressing the 4-1BBL co-stimulatory molecule or CCL21. 4-1BBL and CCL21 expression was identified by FACS analysis and immunoblotting. rV-4-1BBL vaccination shows significant tumor regression compared to rV-LacZ, but rV-CCL21 shows rapid tumor growth compared to rV-LacZ in the poorly immunogenic B16 murine melanoma model. 4-1BBL expression resulted in the increase of the number of CD8+ T cells and especially the increase of effector (CD62L-CD44+) CD8+ T cells. These data suggest 4-1BBL may be the potential target for enhancement of tumor immunotherapy.
Keywords
4-1BBL; Gene therapy; Vaccine; Vaccinia virus;
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