• Journal of the Korean Institute of Intelligent Systems
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• v.19 no.6
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• pp.834-839
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• 2009

It is a key element that the problem of emotional feature extraction based on facial image to recognize a human emotion status. In this paper, we propose an Advanced AAM that is improved version of proposed Facial Expression Recognition Systems based on Bayesian Network by using FACS and AAM. This is a study about the most efficient method of optimal facial feature area for human emotion recognition about random user based on generalized HCI system environments. In order to perform such processes, we use a Statistical Shape Analysis at the normalized input image by using Advanced AAM and FACS as a facial expression and emotion status analysis program. And we study about the automatical emotional feature extraction about random user.

• Proceedings of the KIEE Conference
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• 2001.11a
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• pp.69-71
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• 2001

본 논문에서는 FACS(fluorescence activated cell sorting)의 초소형화를 위한 미세 유체소자들을 플라스틱 기판에 집적하여 제작하고 전기삼투를 이용해서 세포가 일렬로 이송되는 특성을 시험한다. 제작된 미세 유체 소자는 유리 하부 기판과 플라스틱 상부 기판 및 전원장치로 구성된다. 상부기판은 세포를 주입하기 위한 샘플 측 레저버와 세포를 운반 및 일렬 이송이 가능하게 하는 버퍼를 저장할 두 개의 레저버가 있고 이들이 배출되는 레저버로 구성된다. 마이크로머시닝 기술을 이용하여 실리콘 기판 위에 미세 채널 몰드를 제작한 후 PDMS(polydimethylsiloxane)로 주물을 제작한다. $O_2$ 플라즈마를 이용하여 유리 기판과 PDMS 주물을 접합하며 제작된 채널에 적색 잉크와 bead를 샘플 측에 충전하고 버퍼 측에 sodium borate를 충전한 후 전기삼투로 구동시킨다. bead가 일렬로 이송되도록 전장을 조절하고 이때의 유속과 유량을 측정한다. 다양한 전장에 따른 실험을 통하여 채널의 구조를 최적화한다.

• Korean Journal of Child Studies
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• v.24 no.6
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• pp.15-31
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• 2003

This study examined the effects of emotion inducing contexts on types of infants smiling. Facial expressions of forty-five 11-to 15-month-old infants were videotaped in an experimental lab with positive and negative emotional contests. Infants' smiling was identified as the Duchenne smile or non-Duchenne smile based on FACS(Facial Action Coding System, Ekman & Friesen, 1978). Duration of smiling types was analyzed. Overall, infants showed more smiling in the positive than in the negative emotional context. Occurrence of Duchenne smiling was more likely in the positive than in the negative context and in the peek-a-boo than in the melody toy condition within the same positive context. Non-Duchenne smiling did not differ by context.

• Journal of Astronomy and Space Sciences
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• v.36 no.1
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• pp.1-9
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• 2019

In this paper we present analysis of current density when the Cluster spacecraft pass the nightside auroral region at about $4-5R_E$ from the center of Earth. The analysis is made when the inter-spacecraft separation is within 200 km, which allows all four spacecraft to be situated inside the same current sheet. On 22 February 2002, two field-aligned current (FAC) events were observed in both the southern and the northern hemispheres. The FACs were calculated with magnetic field data obtained by the four spacecraft using the Curlometer method. The scales of the FACs along the spacecraft trajectory and the magnitudes were hundreds of kilometers and tens of $nA/m^2$, respectively, and both events were mapped to the auroral region in the ionosphere. We also examined reliability of the results with some parameters, and found that our results are adequately comparable with other studies. Nevertheless, some limitations that decrease the accuracy of current estimation exist.

• JSTS:Journal of Semiconductor Technology and Science
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• v.1 no.4
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• pp.239-247
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• 2001

The Fluorescence-Activated Cell Sorter (FACS) is a well-established instrument used for identifying, enumerating, classifying and sorting cells by their physical and optical characteristics. For a miniaturized FACS device, a disposable plastic microchip has been developed which has a hydrodynamic focusing chamber using soft lithography. As the characteristics of the spatially confined sample stream have an effect on sample throughput, detection efficiency, and the accuracy of cell sorting, systematic fluid dynamic studies are required. Flow visualization is conducted with a laser scanning confocal microscopy (LSCM), and three-dimensional flow structure of the focused sample stream is reconstructed from 2D slices acquired at $1\mutextrm{m}$ intervals in depth. It was observed that the flow structure in the focusing chamber is skewed by unsymmetrical velocity profile arising from trapezoidal cross section of the microchannel. For a quantitative analysis of a microscopic flow structure, Confocal Micro-PIV system has been developed to evaluate the accelerated flow field in the focusing chamber. This study proposes a method which defines the depth of the measurement volume using a detection pinhole. The trajectories of red blood cells (RBCs) and their interactions with surrounding flow field in the squeezed sample stream are evaluated to find optimal shape of the focusing chamber and fluid manipulation scheme for stable cell transporting, efficient detection, and sorting

• Journal of Microbiology
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• v.40 no.4
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• pp.295-300
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• 2002

Previously, we reported induced expression of developmentally regulated CTP-binding protein 2 (DRG2) in fish cells at the late stage of rhabdovirus infection. To investigate the biological role of fish DRG2 (fDRG2), we transfected CHSE-214 cells with an expression vector containing complete fDRG2 fused to the N-terminal end of an enhanced green fluorescent protein (EGFP). Low level expression of fDRG2-EGFP did not induce morphological change or cell death. However, a high level expression of fDRG2-EGFP induced cell rounding and caused depletion of the cell population in FACS analysis. Several truncated fragments were fused to EGFP. FACS analysis was conducted to determine the presence of cells expressing high levels of the resulting chimera. While cells expressing a high level of N-terminus were detected, those expressing high levels of the C-terminal fragment 243-290 containing the G4 motif were absent in FACS analysis. Based on these observations, we propose that overexpression of fDRG2 may induce cell rounding, a representative cytopathic effect of virus-infected cells in the late stage of infection and the C-terminus of the fDRG2 is essential for this function.

• Development and Reproduction
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• v.14 no.3
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• pp.155-162
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• 2010

The trophectoderm is one of the earliest cell types to differentiate in the forming placenta. It is an important for the initial implantation and placentation during pregnancy. Trophoblast stem cells (TBSCs) develop from the blastocyst and are maintained by signals emanating from the inner cell mass. However, several limitations including rarity and difficulty in isolation of trophoblast stem cells derived from blastocyst still exist. To establish a model for trophoblast differentiation, we isolated TBSCs from human term placenta ($\geq$38 weeks) and characterized. Cell cycle was analyzed by measuring DNA content by FACS analysis and phenotype of TBSCs was characterized by RT-PCR and FACS analysis. TBSCs have expressed various markers such as self-renewal markers (Nanog, Sox2), three germ layer markers (hNF68, alpha-cardiac actin, hAFP), trophoblast specific markers (CDX-2, CK7, HLA-G), and TERT gene. In FACS analysis, TBSCs isolated from term placenta showed that the majority of cells expressed CD13, CD44, CD90, CD95, CD105, HLA-ABC, cytokeratin 7, and HLA-G. Testing for CD31, CD34, CD45, CD71, vimentin and HLA-DR were negative. TBSCs were shown to decrease the growth rate when cultured in conditioned medium without FGF4/heparin as well as the morphology was changed to a characteristic giant cell with a large cytoplasm and nucleus. In invasion assay, TBSCs isolated from term placenta showed invasion activities in in vivo using nude mice and in vitro Matrigel system. Taken together, these results support that an isolation potential of TBSCs from term placenta as well as a good source for understanding of the infertility mechanism.

• Proceedings of the Korean Society for Emotion and Sensibility Conference
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• 2012.05a
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• pp.63-64
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• 2012

• YAKHAK HOEJI
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• v.43 no.2
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• pp.278-284
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• 1999

This study deals with the biological changes in mice after ${\gamma}-irradiated$. Four weeks old BALA/c mice were irradiated with 6.5Gy of ${\gamma}-ray$ on the fifth day after oral administration of radioprotectants such as ascorbic acid, tocopherol and cysteine. Control group was irradiated with 6.5Gy without pre-administration of radioprotectors. Blood cells and sperm cells were counted and body, testis and spleen were weighed 3 days after irradiation. And also liver antioxidant activity and range of spleen immune cells were measured. Differences in most biological parameters were not clearly distinguished between experimental groups. However, the relative spleen weight, the relative testis weight and the population size of spleen immune cells such as T helper cells, B cells and macrophages measured by means of FACS showed significant difference between irradiated and radioprotectant administered group. It is concluded that the relative spleen weight, the relative testis weight and the population size of spleen immune cells are easy and useful parameters for assessing the effect of radioprotective substances and for quantifying biological damage of radiation, as well.

• Childhood Kidney Diseases
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• v.22 no.1
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• pp.22-27
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• 2018

Purpose: Podocytes are important architectures that maintain the crucial roles of glomerular filtration barrier functions. Despite this structural importance, however, the mechanisms of the changes in podocytes that can be an important pathogenesis of minimal change nephrotic syndrome (MCNS) are not clear yet. The aim of this study was to investigate whether apoptosis is induced by interleukin (IL)-13 in cultured human podocytes. Methods: Human podocytes were treated with different IL-13 doses and apoptotic cells were analyzed using terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL assay) and fluorescence-activated cell sorting (FACS). Results: The IL-13 increased the number of TUNEL-positive cells in a dose-dependent manner at 6 and 18 hours (P<0.05 and P<0.05, respectively). The apoptosis rate was appeared to be increased slightly in the IL-13-stimulated podocytes (8.63%, 13.02%, and 14.46%; 3, 10 and 30 ng/mL, respectively) than in the control cells (7.66%) at 12 hours by FACS assay. Conclusion: Our study revealed that IL-13 expression may increase podocyte apoptosis. Blocking the IL-13 signal pathway can potentially play an important role in regulating the apoptosis of podocytes.