• Title/Summary/Keyword: F344

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Whole Genomic Expression Analysis of Rat Liver Epithelial Cells in Response to Phenytoin

  • Kim, Ji-Hoon;Kim, Seung-Jun;Yeon, Jong-Pil;Yeom, Hye-Jung;Jung, Jin-Wook;Oh, Moon-Ju;Park, Joon-Suk;Kang, Kyung-Sun;Hwang, Seung-Yong
    • Molecular & Cellular Toxicology
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    • v.2 no.2
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    • pp.120-125
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    • 2006
  • Phenytoin is an anti-epileptic. It works by slowing down impulses in the brain that cause seizures. The recent microarray technology enables us to understand possible mechanisms of genes related to compounds which have toxicity in biological system. We have studied that the effect of a compound related to hepatotoxin in vitro system using a rat whole genome microarray. In this study, we have used a rat liver epithelial cell line WB-F344 and phenytoin as a hepatotoxin. WB-F344 was treated with phenytoin for 1 to 24 hours. Total RNA was isolated at times 1, 6 and 24h following treatment of phenytoin, and hybridized to the microarray containing about 22,000 rat genes. After analysis with clustering methods, we have identified a total of 1,455 differentially expressed genes during the time course. Interestingly, about 1,049 genes exhibited differential expression pattern in response to phenytoin in early time. Therefore, the identification of genes associated with phenytoin in early response may give important insights into various toxicogenomic studies in vitro system.

Genotoxicity of cadmium chloride in Salmonella typhimurium and rat liver epithelial cells (카드뮴의 Salmonella typhimurium 변이균주 및 랫드 간장 상피세포에서의 유전독성)

  • Jeong, Sang-hee;Cho, Myung-haing;Cho, Joon-hyoung
    • Korean Journal of Veterinary Research
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    • v.38 no.3
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    • pp.606-613
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    • 1998
  • Cadmium is one of the well-known environmental toxicants and induces cancer in rodents and human, but its carcinogenic mechanism has not been well demonstrated until now. Genotoxic effects of cadmium in Salmonella typhimurium TA98, TA100 and TA1535/pSK1002 or in WB-F344 rat liver epithelial cells were investigated to elucidate the tumor initiating effects of cadmium. TA98, TA100 and TA1535/pSK1002 tester strains were used to detect frameshift mutation, base-pair mutation and SOS repair response, respectively, in Salmonella mutation test. Reverse mutations from histidine to $histidin^+$ of Salmonella typhimurium TA98 and TA100 by $CdCl_2$ were not significantly different from control up to the maximum doses ($100{\mu}M$ and $200{\mu}M$ in TA98 and TA100, respectively) at which non-cytotoxicity was observed. DNA SOS repair responses(${\beta}$-galactosidase activity) generally did not show significant increases compared to control in both of the conditions with or without metabolic activation in Salmonella typhimurium TA1535/pSK1002 by $CdCl_2$. But the activities of ${\beta}$-galactosidase by $400{\mu}M$ of $CdCl_2$ in metabolic activation condition and by 130 and $400{\mu}M$ of $CdCl_2$ in non-metabolic activation condition were more decreased than those of control. DNA single strand breaks for 4hrs were observed only in WB-F344 rat liver epithelial cells treated with $200{\mu}M$ of $CdCl_2$. As a conclusion, $CdCl_2$ did not induce gene mutation in microbials but induce DNA single strand breaks in rat liver epithelial cells.

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Chemopreventive Effects of Elm Tree Root Extract on Colonic Aberrant Crypt Foci Induced by 1,2-Dimethylhydrazine in F344 Rats

  • Kwon, Hyun-Jung;Kim, Tae-Myoung;Ryu, Jae-Myun;Son, Seung-Hwan;Hong, Jin-Tae;Jeong, Heon-Sang;Kang, Jin-Seok;Ahn, Ji-Yun;Kim, Sung-Ran;Ha, Tae-Youl;Kim, Dae-Joong
    • Preventive Nutrition and Food Science
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    • v.13 no.3
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    • pp.157-165
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    • 2008
  • Cancer-preventive effects of ethanol extract of elm tree root (EEE) were investigated. In the in vitro cytotoxicity assay, colon cancer cells were incubated with a chloroform fraction of EEE (CF-EEE). CF-EEE significantly inhibited the proliferation of cells and induced apoptotic cell death in a dose-dependent manner. For the assessment of chemopreventive efficacy in vivo, male F344 rats were fed with EEE (0.5 or 1%) in diet for 8 weeks, and were subcutaneously injected with 1,2-dimethylhydrazine (DMH) to induce colonic aberrant crypt foci (ACF). EEE (0.5 and 1%) significantly decreased both the numbers of AC (1191.1/colon) and ACF (529.3/colon) induced by DMH. In addition, in the Western blot analysis on the colonic mucosa, administration of EEE triggered expression of caspase-3, a key factor of an apoptotic cascade. These results suggest that extract of elm tree root may have potential chemopreventive principles that lead to apoptosis of cancer cells, and thereby suppress colorectal carcinogenesis during the initiation stage.

Influence of Upper extremity function, Activities of Daily Living, Therapeutic Flow and Quality of Life in Stroke patients (뇌졸중 환자의 상지기능, 일상생활활동, 치료적 몰입이 삶의 질에 미치는 영향)

  • Kim, Ji-Hoon
    • Journal of Digital Convergence
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    • v.16 no.12
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    • pp.417-425
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    • 2018
  • The purpose of this study was to analyzed the correlation between upper extremity function, activities of daily living, quality of life and therapeutic flow in stroke clients and identify the factors influencing therapeutic flow. Total 249 stroke patients from 13 hospitals were measure at therapist and researcher for upper extremity function, activities of daily living, therapeutic flow and quality of life. Factors that affect the quality of life correlation as s result of positively correlated with upper extremity function(r=.312, p<.001), activities of daily living(r=.605, p<.001), therapeutic flow(r=.525, p<.001). And the effect on quality of life in stroke clients variables affecting therapeutic flow(${\beta}=.344$, p<.001), activities of daily living(${\beta}=.293$, p<.05) and Time since of onset(${\beta}=.145$, p<.05) were the order of analysis. The regression model explained 35.9% of variances(F=35.736, p<.001). Therefore, it was found that therapeutic flow in stroke clients is an important factor for quality of life, future therapeutic flow study will continue to be needed.

Population Parameters and Biomass of the Shiba Shrimp Metapenaeus joyneri in Korean Waters (한국산 중하(Metapenaeus joyneri)의 자원생태학적 특성치 및 자원량)

  • Choi, Jung-Hwa;Chang, Dae-Soo;Kim, Jung-Nyun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.39 no.4
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    • pp.344-348
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    • 2006
  • The ecological characteristics and stock biomass of the Shiba shrimp, Metapenaeus joyneri, in Korean waters were determined, using fishery data from the Ministry of Maritime Affairs and Fisheries (MOMAF) and available length frequency data. The instantaneous coefficient of total mortality (Z) of M. joyneri was estimated to be 4.191year, and the annual survival rate (S) was 0.015. The estimated instantaneous coefficient of fishing mortality (F) for recent years was calculated to be 1.94/year. The age at first capture of M. joyneri was 0.71 years. Based on these parameters, the annual biomass of the M. joyneri stock was estimated using a biomass-based cohort analysis and data on the annual catch in weight at age for 1993-2004 in Korean waters. During the study period, the biomass of the shrimp peaked in 1994 at about 9,082 metric tons. Subsequently, it decreased to 500 metric tons in 1998.

Fruit Yield and Quality Evaluation of Sweet Pepper (Capsicum annuum L.) F1 Hybrids Derived from Inbred Lines

  • Shrestha, Surendra Lal;Luitel, Binod Prasad;Lee, Taek Jong;Kang, Won Hee
    • Korean Journal of Breeding Science
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    • v.42 no.4
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    • pp.344-350
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    • 2010
  • Sweet pepper inbred lines (KNU1003, KNU1006, KNU1007, KNU1009, KNU1015, KNU1017 and KNU2006) developed at Kangwon National University (KNU) through conventional means, inbred lines (5AVS1, 5AVS2, 5AVS3, 5AVS5, 5AVS7 and 5AVS8) collected at Rural Development Administration (RDA) and inbred lines (SP12, SP27 and SP14) derived from anther culture were used as female parents and anther culture derived homozygous lines (SP9, SP10, SP14, SP24, SP25, SP27, SP30, SP32, SP34, SP38, SP43, SP45 and SP51) were used as male parents to produce $F_1$ hybrids. A total of 37 $F_1$ hybrids were evaluated for fruit yield and quality characters in summer season, 2007. Variation in fruit number, fruit weight, fruit yield per plant and fruit volume was observed among the $F_1$ hybrids. Superiority on yield over standard/commercial varieties were differed among $F_1$ hybrids. Hybrid $5AVS8{\times}SP45$ exhibited highest heterosis over Special (16.5%) and Fiesta (24.7%). Fruit quality characters (fruit length, fruit width, pericarp thickness, total soluble solid, fruit shape and fruit color) were varied among the $F_1$ hybrids. Fruit number, fruit weight and fruit volume per plant were correlated with fruit yield. Based on the standard heterosis expressed by the hybrids and quality characters evaluation, $KNU1017{\times}SP27$, $5AVS1{\times}SP43$, $5AVS5{\times}SP27$, $5AVS8{\times}SP45$, $SP12{\times}SP38$ and $SP27{\times}SP25$ hybrids were found to be superior over commercial cultivars and are selected. Inbred lines of these hybrid combinations can be used to produce $F_1$ hybrid seed for commercial production.

Anti-tumor Effect of Korean Mistletoe Extract Intensified with Mistletoe Lectin against Melanoma Cells in vitro and in vivo (Lectin으로 강화한 한국산 겨우살이 추출물의 in vitro 및 in vivo에서의 피부암에 대한 항암효과)

  • Yang, Eun-Young;Yeo, Jeong-Hoon;Jin, Ji-Young;Kim, Hyun-Sung;Park, Won-Bong;Suh, Jung-Jin;Hwang, Suk-Yeon
    • Korean Journal of Pharmacognosy
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    • v.34 no.3 s.134
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    • pp.218-222
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    • 2003
  • The mistletoe lectins are major active components in the extrct of Viscum album var. coloratum. This study was performed to investigate the anti- skin cancer effect of Korean mistletoe extract intensified with mistletoe lectin (KI 2103S). B16F10 melanoma cells were allografted in C57BL/6 mice and F344 rats. The effect of KI 2103S on melanoma was measured by monitoring tumor index. The KI 2103S was injected intra-tumorally and tumor index was decreased in dose dependent manner.

The Effects of Diversity Management in a Deluxe Hotel on F & B Employees' Job Engagement and Organizational Commitment (특급 호텔의 다양성 관리가 식음료 종사원의 직무열의 및 조직몰입에 미치는 영향)

  • Jung, Hyo Sun;Yoon, Hyo Sil;Yoon, Hye Hyun
    • Korean journal of food and cookery science
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    • v.32 no.3
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    • pp.363-369
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    • 2016
  • Purpose: The purpose of this study was to understand the interrelationships among diversity management, employees' job engagement, and organizational commitment in a deluxe hotel. Methods: In a total of 344 F & B employees obtained from empirical research, this study reviewed the reliability and fitness of the research model and verified a total of 4 hypotheses using the Amos program. Results: The proposed model provided an adequate fit to the data, $x^2$=316.722 (df=112), CMIN/df=2.828, GFI=0.899, NFI=0.941, IFI=0.961, TLI=0.953, CFI=0.961, RMSEA=0.073. SEM results showed that diversity management (building cultural awareness: ${\beta}$=0.206; pragmatic management policy; ${\beta}$=0.315) had a positive and significant influence on job engagement. Also, employees' job engagement (${\beta}$=0.623) had significant positive effects on organizational commitment. Conclusion: This study shows diversity management of hotel employees and its effectiveness as well as which diversity management is needed positive attitude.

Average and Effective Energies, and Fluence-Dose Equivalent Conversion Factors for $^{239}Pu-Be,\;^{241}Am-Li\;and\;^{241}Am-F$ Neutron Sources

  • Ro, Seung-Gy;Yoo, Young-Soo
    • Nuclear Engineering and Technology
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    • v.3 no.3
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    • pp.155-160
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    • 1971
  • Average and effective energies for 239Pu-Be, 241Am-Li and 241Am-F neutron sources have been calculated from a number of published data for the neutron spectra and for the dose equivalent as a function of neutron energies by a numerical method. Also a calculation of the dose equivalent conversion factors, i. e., the first collision dose equivalent and the surface (or multicollision) dose equivalent that equals the product of surface-absorbed dose and a corresponding quality factor, per unit fluence of neutrons from these sources has been carried out in the same way as before. The results are as follows : 1. for average energies 4.07$\pm$0.33, 0.42 and 1.41 MeV; 2. for effective energies based on the concept of the first collision process in the human body 4.45$\pm$0.344, 0.51 and 1.47 MeV; 3. for effective energies based on the concept of the multi-collision process in the human body 4.50$\pm$0.36, 0.50 and 1.45 MeV; 4. for fluence-first collision dose equivalent conversion factors (2.74$\pm$0.07)10$^{-8}$ , 1.58$\times$ 10$^{-8}$ and 2.34$\times$10$^{-8}$ rems/(n/$\textrm{cm}^2$); and 5. for fluence-surface dose equivalent conversion factors (3.55$\pm$0.09)10$^{-8}$ , 2.19$\times$10$^{-8}$ and 2.82$\times$10$^{-8}$ rems/(n/$\textrm{cm}^2$) : respectively.

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Effects of Adenosine and $N^6-cyclopentyladenosine$ on Superoxide Production, Degranulation and Calcium Mobilization in Activated Neutrophils (Adenosine과 $N^6-cyclopentyladenosine$이 활성화된 중성호성 백혈구에서 Superoxide 생성, 탈과립과 칼슘동원에 나타내는 영향)

  • Kim, Woo-Jung;Shin, Yong-Kyoo;Han, Eun-Sook;Lee, Chung-Soo
    • The Korean Journal of Pharmacology
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    • v.31 no.3
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    • pp.333-344
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    • 1995
  • The effects of adenosine and $N^6-cyclopentyladenosine$ (CPA) on superoxide production, myeloperoxidase release and $Ca^{2+}$ mobilization stimulated by fMLP in neutrophils were investigated. The effects were also observed on the stimulatory actions of C5a and PMA and the responses in lipopolysaccharide-primed neutrophils. In addition, the involvement of cAMP in the inhibitory action of adenosine was examined. The fMLP-stimulated neutrophil respiratory burst, degranulation and intracellular $Ca^{2+}$ mobilization may be regulated by activation of adenosine receptors. Adenosine may not affect the stimulated neutrophil responses due to activation of protein kinase C. fMLP-stimulated respiratory burst in lipopolysaccharide-primed neutrophils may be less sensitive to adenosine, compared with nonprimed cells. The inhibitory effect of theophylline in the presence of adenosine on neutrophil responses appears to be ascribed to accumulation of intracellular cAMP.

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