• Research in Plant Disease
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• v.21 no.2
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• pp.74-81
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• 2015

The specific and sensitive nested-PCR method to detect Acidovorax citrulli, a causal agent of bacterial fruit blotch on cucurbitaceae, was developed. PCR primers were designed from the draft genome sequence which was obtained with the Next Generation Sequencing of A. citrulli KACC10651, and the nested-PCR primer set (Ac-ORF 21F/Ac-ORF 21R) were selected by checking of specificity to A. citrulli with PCR assays. The selected nested-PCR primer amplified the 140 bp DNA only from A. citrulli strains, and detection sensitivity of the nested PCR increased 10,000 times of $1^{st}$ PCR detection limit (10 ng genomic DNA/PCR). The nested PCR detected A. citrulli from the all samples of seed surface wash (external seed detection) of the artificially inoculated watermelon seeds with $10^1cfu/ml$ and above population of A. citrulli while the nested PCR could not detected A. citrulli from the mashed seed suspension (internal seed detection) of the all artificially inoculated watermelon seeds. When the naturally infested watermelon seeds (10% seed infested rate with grow-out test) used, the nested PCR detected A. citrulli from 2 seed samples out of 10 replication samples externally and 5 seed samples out of 10 replication samples internally. We believe that the nested-PCR developed in this study will be useful method to detect A. citrulli from the Cucurbitaceae seeds.

• Journal of Advanced Marine Engineering and Technology
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• v.38 no.6
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• pp.602-608
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• 2014

Hydrogen energy, a field of low-carbon substitute energy, can be produced by fossile fuel reforming and electrolysis of water etc. We developed 1kW class flat type reformer for PEM Fuel Cells. The PEMFC is highly sensitive to carbon monoxide because CO has detrimental effects on the performance of the fuel cell. Thus, reformed gas supplied to Fuel cell system, which maintained CO concentration below 10ppm. After applying optimum drive condition, reformed gas was measured with gas chromatography and could find out about each experimental condition of $H_2$ and CO concentration. As a results, The 1kW class plate type hydrogen generation system's optimum condition is A/F ratio ${\alpha}=1.3$, STR temperature 1023K, S/C ratio 3, and $PrOx1{\cdot}2$ 30cc/min. It turns out that installation of PrOx 2 stage is more efficient for reducing CO concentration.

• Korean Journal of Optics and Photonics
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• v.8 no.3
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• pp.175-182
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• 1997

To design a high resolution three-mirror telescope for visible spectral region, initial design technique based on the generation of flat field anastigmatic solutions limited by mechanical structure was proposed. An initial design of the three-mirror telescope that features relatively high focal ratio(F/14.5), large aperture diameter(600 mm), and squared field of view(0.27$^{\circ}$$\times$1.3$^{\circ}$) was done using an initial design program. Code V was used to optimized the initially designed optical system. As a result, MTF value of the telescope was about 0.5 at Nyquist frequency in each field except for the edge of the CCD.

• Annual Conference on Human and Language Technology
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• 2022.10a
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• pp.374-378
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• 2022

대화형 관계 추출의 목표는 주어진 대화에서 두 개체 간의 관계를 식별하는 것이다. 대화 중에 화자는 개체 및 관계와 관련이 있는 단서인 트리거를 통해 특정 개체 간 관계를 식별하는 것에 힌트를 얻을 수 있다. 그러나 데이터에 대해 항상 트리거 정보가 존재하는 것이 아니므로 트리거를 활용해 성능을 향상시키는 것은 어렵다. 본 논문은 이 문제점을 해소하기 위해 대화, 개체, 관계 중심으로 트리거 생성 모델을 학습하고, 이를 통해 생성된 트리거를 대화형 관계 추출에 학습하여 관계 식별에 효과적인 성능 향상을 보이는 접근법을 제안한다. 제안하는 접근법은 대화형 관계 추출 태스크에서 기존 성능과 비교한 결과 Dev, Test에서 각각 F1 19.74%p, F1 15.53%p 의 성능 향상을 보였다.

• Journal of Families and Better Life
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• v.29 no.5
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• pp.121-140
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• 2011

The purpose of this study is to understand how the economic conditions and needs of single-parent families are different between single-mother and single-father families, and also how they are dependent on household composition. The data for this study were drawn from the 1st Korea Welfare Panel Study and analyzed by frequencies, means, ${\chi}^2$, t-test, F-test, and logistic regression with the STATA 9.1 program. The major findings are as follows: (1) Single-parent families are more likely to live in a three-generation household than married couple families. (2) The composition of a three-generation household of single-parent families is affected by sex, age, education, type of marital disruption, the type of employment of single parents and the age of the last-born child. (3) The income-to-needs ratio is not significantly different depending on the sex of the single parents and their household composition. However, material hardship is significantly low in three-generation household single-parent families. (4) There are differences between three-generation single-parent families and independent single-parent families in income sources: The ratio of public transfer to total incomes is higher in three-generation households than independent households, while the ratio of private transfer to total incomes is higher in independent households.

• Korean Chemical Engineering Research
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• v.47 no.1
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• pp.11-16
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• 2009

Composite electrodes for redox supercapacitor were prepared potentiodynamically by the deposition of $RuO_2$ and the co-deposition of Ru-Co mixed oxide on the surface of single-walled carbon nanotube. Electrode of Ru-Co mixed oxide, in which Ru(13.13 wt%) and Co(2.89 wt%) were deposited on the carbon nanotube, exhibited a similar specific capacitance(${\sim}620\;F\;g^{-1}$) with $RuO_2$ electrode at a low potential scan rate($10\;mV\;s^{-1}$), but showed a superior one ($570\;F\;g^{-1}$) at a high scan rate($500\;mV\;s^{-1}$) than that of $RuO_2$($475\;F\;g^{-1}$). Such increase in the specific capacitance at high scan rate by the co-deposition of Ru and Co species was due to the structural support of Co species to provide the electronic conduction through Ru species.

• Transactions of the Korean Society of Mechanical Engineers
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• v.14 no.2
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• pp.298-309
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• 1990

In this paper, a new method is suggested to analyze impulsive stresses at loading poing of concentrated impact load under certain impact conditions determined by impact velocity, stiffness of plate and mass of impact body, etc. The impulsive stresses are analyzed by using the three dimensional dynamic theory of elasticity so as to analytically clarify the generation phenomenon of cone crack at the impact fracture of fragile materials (to be discussed if the second paper). The Lagrange's plate theory and Hertz's law of contact theory are used for the analysis of impact load, and the approximate equation of impact load is suggested to analyze the impulsive stresses at the impact point to decide the ranage of impact load factor. When impact load factors are over and under 0.263, approximate equations are suggested to be F(t)=Aexp(-Bt)sinCt and F(t)=Aexp(-bt) {1-exp(Ct)} respectively. Also, the inverse Laplace transformation is done by using the F.F.T.(fast fourier transform) algorithm. And in order to clarity the validity of stress analysis method, experiments on strain fluctuation at impact point are performed on a supported square glass plate. Finally, these analytical results are shown to be in close agreement with experimental results.

• Korean Journal of Remote Sensing
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• v.38 no.6_1
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• pp.1109-1124
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• 2022

This study proposes a marine debris monitoring methods using satellite and drone multispectral images. A multi-layer perceptron (MLP) model was applied to detect marine debris using Sentinel-2 satellite image. And for the detection of marine debris using drone multispectral images, performance evaluation and comparison of U-Net, DeepLabv3+ (ResNet50) and DeepLabv3+ (Inceptionv3) among deep learning models were performed (mIoU 0.68). As a result of marine debris detection using satellite image, the F1-Score was 0.97. Marine debris detection using drone multispectral images was performed on vegetative debris and plastics. As a result of detection, when DeepLabv3+ (Inceptionv3) was used, the most model accuracy, mean intersection over union (mIoU), was 0.68. Vegetative debris showed an F1-Score of 0.93 and IoU of 0.86, while plastics showed low performance with an F1-Score of 0.5 and IoU of 0.33. However, the F1-Score of the spectral index applied to generate plastic mask images was 0.81, which was higher than the plastics detection performance of DeepLabv3+ (Inceptionv3), and it was confirmed that plastics monitoring using the spectral index was possible. The marine debris monitoring technique proposed in this study can be used to establish a plan for marine debris collection and treatment as well as to provide quantitative data on marine debris generation.

• Korean Journal of Clinical Laboratory Science
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• v.39 no.3
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• pp.249-255
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• 2007

While respiratory burst enhances neutrophil glucose utilization, many neutrophil functions are critically influenced by extracellular matrix interaction and phosphoinositide-3-OH kinase (PI3K) signaling. We thus evaluated the role of RGD integrin occupancy and PI3K inhibition on respiratory burst and [18F]FDG uptake of stimulated neutrophils. Human neutrophils were stimulated by 100 ng/mL phorbol-myristate-acetate (PMA), and respiratory burst was measured by cumulative luminescence with lucigenin. [18F]FDG uptake and total hexokinase activity was measured 20 min after PMA stimulation in the presence or absence of soluble RGD peptides (200 g/mL) and/or the PI3K inhibitor wortmannin (200 nM). PMA induced a 71.70.9 fold increase in neutrophil oxygen intermediate generation. [18F]FDG uptake was increased to $194.6{\pm} 3.7%$ and hexokinase activity to $145.0{\pm}2.0%$ of basal levels (both p<0.0005). RGD peptides attenuated respiratory burst activation to $35.6{\pm}0.2%$ (p<0.005), but did not inhibit stimulated [18F]FDG uptake or hexokinase activity. In contrast, without affecting respiratory burst activation, wortmannin inhibited PMA stimulated [18F]FDG uptake to $66.9{\pm}1.6%$ and hexokinase activity to $81.0{\pm}4.2%$ (both P<0.0005), demonstrating its dependence on PI3K activity. Neither RGD nor wortmannin reversed the other's inhibitory effect on stimulated [18F]FDG uptake and hexokinase activity or respiratory burst, which suggests the involvement of distinct signaling pathways. Neutrophil [18F]FDG uptake is enhanced by PMA through a mechanism that requires PI3K activity but is independent of integrin receptor occupancy or respiratory burst activation.

• International Journal of Industrial Entomology and Biomaterials
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• v.8 no.1
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• pp.113-115
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• 2004

Six generations (P$_1$, P$_2$, F$_1$, BC$_1$, BC$_2$ and F$_2$) of four bivoltine silkworm crosses (SKAU-R-1 ${\times}$ Yakwei, SKAU-R-6 ${\times}$ Yakwei, CSR$_2$ ${\times}$ CSR$_4$ and SH$_{6}$${\times}$ NB$_4$D$_2$) were evaluated in a completely randomized block design with 5 replications for each treatment. The generation mean 2 in respect of 3 metric traits (single cocoon weight, single shell weight, and shell ratio ％), were subjected to Cavallis joint scaling test. Additive dominance model was found to be adequate in CSR$_2$${\times}$CSR$_4$ and SH$_{6}$${\times}$ NB$_4$D$_2$ for single cocoon weight and shell weight and SKAU-R-6 ${\times}$ Yakwei for shell ratio(％). Whereas, in rest of the crosses epistasis was evident in the traits under investigation. Magnitude of additive gene effect (d) was greater than dominance(h) in SH$_{6}$${\times}$NB$_4$D$_2$ and SKUA-R-6${\times}$Yakwei for shell ratio (％) and in CSR$_2$${\times}$ CSR$_4$ for shell weight. Thus selection for these traits in early segregating generations of these crosses would be effective for obtaining considerable genetic gain. gain.