• 동력기계공학회지
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• 제5권4호
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• pp.53-60
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• 2001

This paper considers a modeling for the MIMO magnetic bearing system. The rotor is flexible and has a complex shape. To obtain the nominal plant transfer functions, we perform a numerical analysis by using the finite element method(F.E.M.) for the rotor's dynamics, and make a nominal model by reducing the modes from the results. And, we have experimented on the frequency response by a closed-loop identification method, and compared it with the simulation's result on the closed-loop control system.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 1992년도 봄 학술발표회 논문집
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• pp.137-142
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• 1992

The purpose of this study is to develop a new structural system for precast deck plate bridge. In order to investigate load distribution and composite effects by connecting diaphragms and precast deck plate to main girder with shear keys, computer analysis with F.E.M S/W package program ADINA was carried out. As results of analysis, the following could be concluded : -The distance between diaphragms with6m is suficient for crossing load distribution effects. -The composite effects by connection deck plate to main girder with shear key is reasonable. -The gap between deck plate and main girder under given load is negligible.

• 생명과학회지
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• 제15권3호
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• pp.415-422
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• 2005

Mycobacterium hovis BCG 균주에 transposon을 사용하여 mutagenesis를 수행함으로써 mutant library를 제조하였다. 이 mutant library를 screening하여 항결핵제인 PA-824에 내성을 갖는 mutant들을 얻었고, M. bovis wild type에서는 정상적으로 생성되는 coenzyme $F_{420}$이 대부분의 이들 mutant들에서는 생성되지 않는다는 것을 알게 되었다. 세포 추출액을 HPLC로 분석해본 결과, 그 중에서 한 mutant는 $F_{420}$은 생성하지 않으나 그 전구물질인 F0는 생성하고 있음이 밝혀졌다. 따라서 이 mutant 에서는 $F_{420}$생합성 회로의 마지막 단계가 차단되어있음을 알 수 있다. 이 mutant를 inverse PCR을 통해 분석해본 결과, transposon이 Rv2435c유전자에 삽입되어있는 것을 확인할 수 있었다. Rv2435c유전자는 세포막에 결합되어있는 80.3 kDa의 단백질을 암호화하는 것으로 추정되고, 이 단백질의 N-말단은 periplasm에 존재하고 C-말단은 원형질에 존재하는 것으로 추정되고 있다. 원형질에 존재하는 C-말단은 원핵생물과 진핵생물들의 adenylyl cyclase들과 높은 유사성을 나타낸다. Adenylyl cyclase는 ATP로부터 cAMP를 생합성하는 효소이다 M. tuberculosis나 M. bovis의 genome에는 class III adenylyl cyclase를 암호화하는 것으로 추정되는 유전자가 모두 15개나 존재한다 특히 이들 중에서 Rv1625c 와 Rv2435c는 포유류의 adenylyl cyclase들과 높은 유사성을 가지는 것으로 알려져 있다 이 Rv2435c 단백질이 진정한 adenylyl cyclase인지를 확인하기 위하여 우리는 이 단백질 중에서 원형질에 존재하는 부분을 말단에 6개의 histidine을 첨부한 채로 대장균에서 발현시켰다. 대장균에서 이 단백질이 생성되는 것은 histidine이 첨부된 단백질을 Ni-NTA resin을 사용하여 대장균으로부터 분리함으로써 확인하였다. 그러나 이 단백질이 대장균에서 cya mutation을 complementation하지 못하였고, 따라서 이 단백질이 adenylyl cyclase 활성을 갖지 않음을 알 수 있었다. 자외선이나 hydroxylamine을 사용한 mutagenesis 또는 Rv2435c와 Rv1625c간의 토sion단백질을 만들어서, 이 단백질이 adenylyl cyclae로서의 활성을 획득하도록 하는 모든 시도는 실패하였다. 따라서 Rv2435c단백질이, F0가 $F_{420}$으로 변환되는 데에 영향을 미치는 방법이 cAMP를 생성함으로써가 아니라 다른 방법으로 영향을 미치고 있다는 것을 알 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제18권4호
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• pp.648-657
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• 2008

In this study, we used functional genomic strategies, proteomics and quantitative real-time (qRT)-PCR, to advance our understanding of genes associated with fumonisin production in the fungus Fusarium verticillioides. Earlier studies have demonstrated that deletion of the FCC1 gene, which encodes a C-type cyclin, leads to a drastic reduction in fumonisin production and conidiation in the mutant strain (FT536). The premise of our research was that comparative analysis of F. verticillioides wild-type and FT536 proteomes will reveal putative proteins, and ultimately corresponding genes, that are important for fumonisin biosynthesis. We isolated proteins that were significantly upregulated in either the wild type or FT536 via two-dimensional polyacrylamide gel electrophoresis, and subsequently obtained sequences by mass spectrometry. Homologs of identified proteins, e.g., carboxypeptidase, laccase, and nitrogen metabolite repression protein, are known to have functions involved in fungal secondary metabolism and development. We also identified gene sequences corresponding to the selected proteins and investigated their transcriptional profiles via quantitative real-time (qRT)-PCR in order to identify genes that show concomitant expression patterns during fumonisin biosynthesis. These genes can be selected as targets for functional analysis to further verify their roles in $FB_1$ biosynthesis.

• 한국산림과학회지
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• 제107권4호
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• pp.385-392
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• 2018

석산개발 지역 퇴적장 석축사면의 사면 조성 및 관리기술 증진을 위하여 현황측량을 실시하고, 대표단면을 선정하여 사면의 안정성 해석을 수행한 결과를 요약하면 다음과 같다. 사면안정 해석을 위하여 선정한 대표단면의 퇴적장 최하단부 사면(A) 경사는 $59^{\circ}$로서 급경사를 이루고 있으나, 전체 퇴적장 사면(A, B, C, D, E, F)의 평균경사는 $38.5^{\circ}$이었다. 퇴적장 사면의 수평거리는 총 66.2 m, 사면높이는 48.3 m이지만 퇴적장 석축사면의 경사는 비교적 급하며, 시각적으로도 불안정해 보인다. 사면안정 해석에는 건조시와 포화시를 고려하였으며 포화시의 사면안정 해석은 대상사면이 완전히 포화된 상태를 모델링하여 수행하였다. 지반의 강도정수는 석축과 배면지반으로 구분하고 안전성을 검토하였다. 건기시 안전율은 1.850, 우기시 안전율은 1.333으로 나타나 건기시 및 우기시의 안전율 1.5와 1.2를 상회하여 사면의 활동에 대하여 모두 안전한 것으로 나타났다. 그러나 집중호우 시 퇴적장 사면 상부의 풍화된 마사토는 사면침식과 붕괴 위험성이 높아 배수로 설치 등 적절한 유지관리를 통한 안정대책이 필요하다고 생각된다.

• 한국안전학회지
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• 제28권2호
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• pp.26-30
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• 2013

The purpose of this study is to find risk factors by analyzing the operation principle of a photo coupler (P/C) used to remove the noise of electronic devices and establish a base for the performance improvement of developed products. It was found from the P/C circuit analysis of normal products that they were equipped with an electrolytic condenser of $0.1{\mu}F$ to smooth system signals. Due to the epoxy resin packing the external part of the P/C, this study experienced a limit to visually examine the damage to it. It could be seen from the analysis of electric characteristics of the P/C that the forward voltage ($V_f$) and reverse current ($I_r$) were 1.3 V and 10 uA, respectively. In addition, it is required that the breakdown voltage (VCE) between the collector (C) and emitter (E) be maintained at less than 35 V. The and of the damaged product #1 were comparatively good. However, the measurement of was 100.0 uA. From this, it is thought that a short circuit occurred to the internal circuit. Moreover, from the fact that the of the damaged product #2 was open circuit and the measurement of was 0.0 uA, it is thought that the collector and emitter was separated or insulation resistance was significantly high. Furthermore, from the fact that the of the damaged product #3 was open circuit and the measurement of was 0.0 uA, it is thought that the space between the collector (C) and emitter (E) failed to meet the design standard or that they were separated. Therefore, it is thought that fabricating the P/C by increasing the reverse current 10 mA to 50 mA will prevent its malfunction.

• Journal of Welding and Joining
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• 제17권1호
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• pp.88-96
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• 1999

The effects of root gap on welding residual stress and deformation are dealt with the multi-pass weldment with three kinds(0, 6, 30mm) of root gap by F.E.M common code, and then compared with experiment data. In this analysis, an 100% ramp heat input model was used to avoid numerical convergence problem due to an instantaneous increase in temperature near the fusion zone, and the effect of a moving arc in a two dimensional plane was also included. During the analysis, a small time increment was applied in a period with instantaneous temperature fluctuation while a large time increment was used in the rest period. The residual stress is distributed as symmetric types and maximum value is also equivalent when the weldment with 0mm and 6mm root gap is welded. In the case of 30mm root gap welding, the distribution of the residual stress extends over a wide range as asymmetric types due to the built-up weld, and most of the residual stress is biased in the side of a built-up weld part. In case of 0mm gap welding and 6mm gap welding, a little angular distortion occurs, but the level of deformation is small. When the weldment with 30mm root gap is welded, the angular deformation of the asymmetric types, however, occurs larger than the other specimens. The experimental and the analytic results show good coincidence and indicate that the welding residual stress and deformation distribution of 30 mm root gap specimen may be asymmetric and the amplitude is larger than those of root gap specimen under standard.

• 한국지반공학회논문집
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• 제24권11호
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• pp.157-166
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• 2008

경량기포혼합토(LAS)의 일축압축강도($q_u$)와 변형계수($E_{50}$)에 영향을 미치는 인자를 분석하기위해 모래, 실트, 점토의 함유율이 서로 다른 건설발생토(원료토)를 이용하여 일축압축강도시험을 수행하였다. 일축압축강도시험 분석결과 동일 원료토에서 LAS의 목표단위중량(${\gamma}_m$) 및 시멘트 첨가량($C_i$)이 강도-변형특성의 지배인자로 분석되었으며, 서로 다른 원료토에서는 단위중량과 시멘트 첨가량이 동일할 경우 원료토의 입도분석시험(KS F2302)결과 산출된 모래, 실트, 점토의 상호비율과 강도-변형특성의 관계를 제시하였다. 양생일에 따른 LAS의 강도발현속도는 원료토내 점토의 함유율($C_%$)이 증가할수록 강도발현을(a)은 저하되는 것으로 나타났다.

• Molecules and Cells
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• 제25권3호
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• pp.417-427
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• 2008

Comparison of maps and QTLs between populations may provide us with a better understanding of molecular maps and the inheritance of traits. We developed and used two reciprocal $BC_1F_1$ populations, IP/DS//IP and IP/DS//DS, for QTL analysis. DS (Dasanbyeo) is a Korean tongil-type cultivar (derived from an indica x japonica cross and similar to indica in its genetic make-up) and IP (Ilpumbyeo) is a Korean japonica cultivar. We constructed two molecular linkage maps corresponding to each backcross population using 196 markers for each map. The length of each chromosome was longer in the IP/DS//IP population than in the IP/DS//DS population, indicating that more recombinants were produced in the IP/DS//IP population. Distorted segregation was observed for 44 and 19 marker loci for the IP/DS//IP and IP/DS//DS populations, respectively; these were mostly skewed in favor of the indica alleles. A total of 36 main effect QTLs (M-QTLs) and 15 digenic epistatic interactions (E-QTLs) were detected for the seven traits investigated. The phenotypic variation explained (PVE) by M-QTLs ranged from 3.4% to 88.2%. Total PVE of the M-QTLs for each trait was significantly higher than that of the E-QTLs. The total number of M-QTLs identified in the IP/DS//IP population was higher than in the IP/DS//DS population. However, the total PVE by the M-QTLs and E-QTLs together for each trait was similar in the two populations, suggesting that the two $BC_1F_1$ populations are equally useful for QTL analysis. Maps and QTLs in the two populations were compared. Eleven new QTLs were identified for SN, SF, GL, and GW in this study, and they will be valuable in marker-assisted selection, particularly for improving grain traits in tongil-type varieties.

• 한국전기전자재료학회논문지
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• 제15권4호
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• pp.332-337
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• 2002

The applicability of models based on fractal geometry to characterize the surface of the EL devices was investigated. Insulating layer and phosphor layer of EL devices were deposited on ITO glass using e-beam method. The images of phosphor layer by scanning electron microscope(SEM) were transformed to binary coded data. The relations between fractal geometry and electrical characteristics of EL devices were investigated. When the fractal dimension of $Cas:EuF_3$ EL device was 1.82 and its grain boundary area was 19%, the brightness of $Cas:EuF_3$ EL device was 261 cd/$\textrm{m}^2$.