• The Plant Pathology Journal
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• 제17권2호
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• pp.101-105
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• 2001

A disease survey on the carnation (Dianthus caryophyllus L.) wilt was conducted during the high temperature period (June through August) and the low temperature period (February through May) in 58 greenhouses of its major cultivation areas, including Pusan, Kimhae, and Changwon in Korea from 1998 to 1999. The disease incidence was averaged 5.4% and 11.9% in the low and high temperature periods, respectively. Severe damage was found in summer with high incidences of around 50% in some greenhouses. Close examination of the symptoms and isolation of the causal agent revealed that there was a new disease different from Fusarium wilt caused by Fusarium oxysporum f. sp. dianthi, which was determined as the stub dieback caused by F. was cetermined as the stub dieback caused by F. graminearum (teleomorph : Gibberella zeae). The stub dieback symptoms involved brown rot of stem that started usually from the portion of cutting without discoloration of inner vascular tissues. Seven out of 38 isolates from the wilted plants were identified as F. graminearum, while the others as F. oxysporum f. sp. dianthi. Mycological characteristics of the stub dieback pathogen including colony color, absence of microconidia, and the shape of macroconidia, were consistent with F. graminearum previously described. This is the first report of the carnation stub dieback in Korea.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 1999년도 임시총회 및 추계 학술발표회
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• pp.30.2-30
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• 1999

• 한국식물병리학회지
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• 제14권6호
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• pp.668-675
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• 1998

강원 고랭지의 대표적 지대인 평창의 대관령, 홍천의 내면 그리고 횡성의 둔내지역에서 재배되고 있는 주요 원예작물에 대해 1994년$\sim$1997년까지 4년에 걸쳐 실시한 병해조사의 결과는 다음과 같다. 1) 배추와 무에서는 순무모자이크바이러스(TuMV)가 '92년부터 고랭지에 다발생하여 '94년과 '96년에 피해가 심하였으며, 이 바이러스병과 함께 무름병의 발생이 심한 편이었으며, 최근에는 무사마귀병의 피해고 '96년부터 점증 추세에 있으며 수년 전까지 피해가 심했던 뿌리 마름병은 최근 발생이 지극히 저조한 점이 특이하다. 거의 모든 채소작물에서 무름병, 잿빛곰팡이병, 노균병, 흰가루병, 잘록병, 검은무늬병 등의 발생이 심한 것이 특징인데 저지대와 달리 고랭지 기후환경과 무관하지 않았을 것이다. 한편 조사기간 중 새로운 미기록종으로는 셀러리의 바이러스(BBWV)와 잿빛곰팡이병(Botrytis cinerea), 메론의 점무늬병(Cercospora citullina), 딸기의 흰가루병(Erysiphe polygori), 양상추의 흰가루병(Erysiphe cichoraceaium) 등이 밝혀졌으며 기주 미기록으로는 파슬리의 무사마귀병(Plasmodiophora brassicae)이 밝혀진 것이 특이하다. 2)여러 가지 화훼작물에는 주로 바이러스병, 잿빛곰팡이병, 시들음병, 흰무늬병 등의 발생이 많았으며 특히 자생 나리류에 흰무늬병(Cercospora spp.) 발생이 심하였다. 미기록종으로는 꽃도라지의 시들음병(Fusarium oxysporum f. sp. eustomae), 바이러스병(BBWV, CMV), 균핵병(Sclerotinia sclerotiorum), 잿빛곰팡이병(B. cinerea), 카네이션의 반점병(Cladosporium echinulatum), 용담의 시들음병(Fusarium oxysporum), 잎마름병(Alternavia dianthi) 등과 Phytoplasma에 의한 스타티스의 빗자루병, 리아트리스의 빗자루병, 자생나리류의 흰무늬병(Cercospora sp.), 스토크의 TuMV 등이 동정되었다.

• Journal of Microbiology and Biotechnology
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• 제18권9호
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• pp.1492-1495
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• 2008

Successful control of Xanthomonas oryzae pv. oryzicola, the causal agent of bacterial leaf streak, requires a specific and reliable diagnostic tool. A pathovar-specific PCR assay was developed for the rapid and accurate detection ofthe plant pathogenic bacterium Xanthomonas oryzae pv. oryzicola in diseased plant. Based on differences in a membrane fusion protein gene of Xanthomonas oryzae pv. oryzicola and other microorganisms, which was generated from NCBI (http://www.ncbi.nlm.nih.gov/) and CMR (http://cmr.tigr.org/) BLAST searches, one pair of pathovar-specific primers, XOCMF/XOCMR, was synthesized. Primers XOCMF and XOCMR from a membrane fusion protein gene were used to amplity a 488-bp DNA fragment. The PCR product was only produced from 4 isolates of Xanthomonas oryzae pv. oryzicola among 37 isolates of other pathovars and species of Xanthomonas, Pectobacterium, Pseudomonas, Burkholderia, Escherichia coli, and Fusarium oxysporum f.sp. dianthi. The results suggested that the assay detected the pathogen more rapidly and accurately than standard isolation methods.

• Journal of Microbiology and Biotechnology
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• 제17권11호
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• pp.1765-1771
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• 2007

A polymerase chain reaction (PCR)-based method was developed to detect the DNA of Ralstonia solanacearum, the causal agent of bacterial wilt in various crop plants. One pair of primers (RALSF and RALSR), designed using cytochrome c1 signal peptide sequences specific to R. solanacearum, produced a PCR product of 932 bp from 13 isolates of R. solanacearum from several countries. The primer specificity was then tested using DNA from 21 isolates of Ralstonia, Pseudomonas, Burkholderia, Xanthomonas, and Fusarium oxysporum f. sp. dianthi. The specificity of the cytochrome c1 signal peptide sequences in R. solanacearum was further confirmed by a DNA-dot blot analysis. Moreover, the primer pair was able to detect the pathogen in artificially inoculated soil and tomato plants. Therefore, the present results indicate that the primer pair can be effectively used for the detection of R. solanacearum in soil and host plants.