• The Korean Journal of Mycology
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• v.18 no.3
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• pp.132-136
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• 1990

The vegetative nuclear divisions in hyphae and the chromosome of Fusarium were observed by use of HCI-Giemsa technique and light microscope. The chromosome of nuclear in F. moniliforme both 7150 and 7219 were eight. F. subglutinans 1082 was n=8 and n=7 in F. sub­glutinans 1083. F. nygamai 5668 was n=7 and n=5 in F. nygamai 7132. F. beomiforme 9758 and 9760 were n=7. F. coccidicola ATCC 24138 and F. acuminatum ATCC 16560 were n=6. From these results and other reports, the basic chromosomal number of these fungi might be speculated to be four.

• The Korean Journal of Mycology
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• v.23 no.4 s.75
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• pp.359-368
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• 1995

F. napiforme, F. beomiforme and F. nygamai could not be classified in any of the existing sections of the genus Fusarium. To discuss of the exact taxonomic relationships among these species, the cellular polypeptide patterns were compared by using 2-D PAGE. Polypeptide pattern of F. beomiforme was different from those of other two species and was more similar to F. oxysporum in section Elegans. F. nygamai and F. napiforme might be another same section which would lie between section Liseola and section Elegans. The results were consistent with the comparison of isoenzyme patterns in these species.

• The Korean Journal of Mycology
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• v.22 no.4
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• pp.386-393
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• 1994

To investigate taxonomical relationships of Fusarium species, $esterase-{\alpha},\;-{\beta}$, acid phosphatase, malate dehydrogenase, peroxidase and polygalacturonase were extracted and the isozyme patterns were compared by using polyacrylamide gel electrophoretic analysis. Only polygalacturonase was monozyme and the other enzymes showed little differences in banding patterns. Genetic similarities based on the isozyme banding patterns were as follows: the interspecific similarity between F. subglutinans and F. moniliforme in Liseola showed the closest relationship of 74.3% of all species studies. And the similarity between section Elegans and section Liseola was 45.4%. F. napiforme and F. nygamai showed the similarity of 64.7%, similar to the correlation between species in the same section. The similarity of these two species to Liseola and Elegans showed 55.2% and 45.4%, being revealed that they would be closer to Liseola than Elegans. However, these results were similar to those of any other sections. Therefore it suggested that these 2 species should be in a different section from any other sections. And F. graminearum showed the similarity of 28.2% to the other 6 species.

• Mycobiology
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• v.30 no.3
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• pp.139-145
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• 2002

Gibberella fujikuroi is species complex. This species complex includes Fusarium tabacinum, F. moniliforme(=F. verticillioides), F. nygamai, F. proliferatum as well as F. subglutinans. Our objective was to develop a technique to differentiate between isolates of F. subglutinans, F. proliferatum and F. verticillioides. Thirty-two strains of F. subglutinans, six strains from F. verticillioides and five strains of F. Proliferatum isolated from maize in Austria were studied using random amplified polymorphic DNA(RAPD). F. subglutinans strains clustered very closely, with similarity ranging from $87{\sim}100%$. On the other hand, all the amplification patterns of F. verticillioides were identical, as well as in the case of F. proliferatum. Our results indicated that these Fusaria species are distinct species and hence RAPD markers can be quick and reliable for differentiating them.

• The Korean Journal of Mycology
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• v.37 no.2
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• pp.121-129
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• 2009

In 2007, a total of 77 isolates of Fusarium spp. were obtained from ear rot symptoms of corns collected from 5 locations in Gangwon Province, Korea. The fungal isolates were identified based on their morphological features. Out of the isolates, fifteen isolates were identified as Fusarium verticillioides which formed microconidia in long chains on monophialides. Four isolates were identified as F. subglutinans which formed microconida only on false heads. Six isolates were identified as F. graminearum which produced red pigment in PDA culture. Besides these Fusarium species, F. napiform, F. nygamai, and F. oxysporum were identified from the rest isolates. To assess for genetic diversity of the isolates, a random amplified polymorphic DNA(RAPD) technique was carried out using URP primers. The results from the RAPD analysis showed that the isolates from corn were divided into 6 groups. These RAPD groups of the Fusarium species corresponded to morphological characters of the Fusarium species. The phylogenetic analysis of most isolates by DNA sequencing of EF-1$\alpha$ gene corresponded to morphological characters of the Fusarium species. The results of pathogenicity tests by two inoculation methods revealed that F. verticillioides, F. graminearum and F. subglutinans are strongly pathogenic to corn stalks.