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http://dx.doi.org/10.4489/KJM.2009.37.2.121

Identification of Fusarium Species Associated with Corn Ear Rot  

Choi, Hyo-Won (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA)
Kim, Jung-Mi (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA)
Kim, Jin-Hee (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA)
Hong, Sung-kee (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA)
Kim, Wan-Gyu (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA)
Chun, Se-Chul (Department of Molecular Biotechnology, College of Life and Environmental Sciences, Konkuk University)
Publication Information
The Korean Journal of Mycology / v.37, no.2, 2009 , pp. 121-129 More about this Journal
Abstract
In 2007, a total of 77 isolates of Fusarium spp. were obtained from ear rot symptoms of corns collected from 5 locations in Gangwon Province, Korea. The fungal isolates were identified based on their morphological features. Out of the isolates, fifteen isolates were identified as Fusarium verticillioides which formed microconidia in long chains on monophialides. Four isolates were identified as F. subglutinans which formed microconida only on false heads. Six isolates were identified as F. graminearum which produced red pigment in PDA culture. Besides these Fusarium species, F. napiform, F. nygamai, and F. oxysporum were identified from the rest isolates. To assess for genetic diversity of the isolates, a random amplified polymorphic DNA(RAPD) technique was carried out using URP primers. The results from the RAPD analysis showed that the isolates from corn were divided into 6 groups. These RAPD groups of the Fusarium species corresponded to morphological characters of the Fusarium species. The phylogenetic analysis of most isolates by DNA sequencing of EF-1$\alpha$ gene corresponded to morphological characters of the Fusarium species. The results of pathogenicity tests by two inoculation methods revealed that F. verticillioides, F. graminearum and F. subglutinans are strongly pathogenic to corn stalks.
Keywords
Corn ear rot; Fusarium graminearum; Fusarium subglutinans; Fusarium verticillioides; Pathogenicity;
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1 Gelderblom, W. C. A, Jaskiewicz, K., Marasas, W. F. O., Thiel, P. G.,Horak, R. M. and Kriek, N. P. J. 1988. Fumonisins-novel mycotoxins with cancer-promoting activity produced by Fusarium moniliforme. Applied and Environmental Microbiology 54:1806-1811.
2 Nelson, P. E., Toussoun, T. A. and Marasas, W. F. O. 1983. Fusarium species: An illustrated manual for identification. Pennsylvania State University Press, University Park, Pennsylvania.
3 Gerlach, W. and Nirenberg, H. 1982. The genus Fusarium - A pictorial atlas. (Mitteilungen aus der biologischen Bundesanstalt Fr Land - und Forstwirtschaft (Berling-Dahlem) 209:1-405.
4 Kini, K. R., Leth, V. and Mathur, S. B. 2002. Genetic variation in Fusarium moniliforme isolated from seeds of different host species from Burkina Faso based on random amplified polymorphic DNA analysis. J. Phytopathology 150:209-212.   DOI   ScienceOn
5 Leslie, J. F. and Summerell, B. A. 2006. The Fusarium laboratory manual. Blackwell publishing. 1-388.
6 Rohlf, F.J. 2000. NTSYS-pc numerical taxonomy and multivariate analysis system, version 2.1. Exeter Publishing, Ltd., Setauket, N.Y.
7 O'Donnell, K., Kistler, H. C., Cigelnik, E. and Ploetz, R. C. 1998b. Multiple evolutionary origins of the fungus causing Panama disease of banana: Concordant evidence from nuclear and mitochondrial gene genealogies. Proc. Natl. Acad. Sci. USA 95:2044-2049.   DOI   ScienceOn
8 Pamphile, J. A. and Azevedo, J., L. 2002. Molecular characterization of endophytic strains of Fusarium verticillioides(= Fusarium moniliforme) from maize(Zea mays. L). World Journal of Microbiology & Biotechnology 18:391-396.   DOI   ScienceOn
9 Presello, D. A., Iglesias, J., Botta, G. and Eyhrabide, G. H. 2007. Severity of Fusarium ear rot and concentration of fumonisin in grain of Argentinian maize hybrids. Crop Protection 26:852-855.   DOI   ScienceOn
10 Silva, E., Mora, E. A., Medina, A., Vasquez, J., Valdez, D.,Danial, D. L. and Parlevliet, J. E. 2007. Fusarium ear rot and how to screen for resistance in open pollinated maize in the Andean regions. Euphytica 153:329-337.   DOI   ScienceOn
11 Sutton, J. C. 1982. Epidemiology of wheat head blight and maize ear rot caused by Fusarium graminearum. Canadian Journal of Plant Pathology 4:195-209.   DOI
12 Miedaner, T. Schilling, A. G. and Geiger, H. H. 2001. Molecular genetic diversity and variation for aggressiveness in populations of Fusarium graminearum and Fusarium culmorum sampled from wheat fields in different countries. J. Phytopathology 149:641-648.   DOI   ScienceOn
13 Miller, J. D., Savard, M. E., Schaafsma, A. W., Seifert, K. A. and Reid, L. M. 1995. Mycotoxin production by Fusarium moniliforme and Fusarium proliferatum from Ontario and occurrence of fumonisin in the 1993 corn crop. Canadian Journal of Plant Pathology 17:233-239.
14 Munkvold, G. P. 2003. Epidemiology of Fusarium diseases and their mycotoxins in maize ears. European Journal of Plant Pathology 109:705-713.   DOI   ScienceOn
15 Lee, Y. W., Kim, K. H. and Chung, H. S. 1990. Production of T-2 toxin and metabolites by Fusarium sporotrichioides isolates from the corn producing area in Korea. Kor. J. Mycol. 18(1):13-19.   과학기술학회마을
16 Aoki, T., O'Donnell, K. and Ichikawa, K. 2001. Fusarium fractiflexum sp. nov. and two other species within the Gibberella fujikuroi species complex recently discovered in Japan that form aerial conidia in false heads. Mycoscience 42:461-478.   DOI   ScienceOn
17 Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequences alignment through sequences weighting, position specific gap penalties and weight matrix choice. Nucleic acids Res. 22:4673-4680.   DOI   ScienceOn
18 서영수, 서정아, 손황배, 이인원. 1998. 옥수수와 보리에서 Fusarium graminearum의 8-ketotrichothecenes 생성. 한국식물병리학회지 14(5): 418-424.   과학기술학회마을
19 Aoki, T. and O'Donnell, K. 1999. Morphological and molecular characterization of Fusarium pseudograminearum sp. nov.,formerly recognized as the group 1 population of F. graminearum. Mycologia 91:597-609.   DOI
20 Kang, H. W., Park, D.S., Go, S.J. and Eun, M.Y. 2002. Fingerprinting of diverse genomes using PCR with universal rice primers generated form repetitive sequence of Korean weedy rice. Mol. Cells 13:281-287.
21 Danielsen, S., Meyer, U. M. and Jensen D. F. 1998. Genetic characteristics of Fusarium verticillioides isolates from maize in Costa Rica. Plant Pathology 47:615-622.   DOI   ScienceOn
22 Seifert, K. A., Aoki, T., Baayen, R. P., Brayford, D., Burgess, L. W.,Chulze, S., Gams, W., Geiser, D., Gruyter, j., Leslie, J. F.,Logrieco, A., Marasas, W. F. O., Nirenberg, H. I., O'Donnell, K.,Rheeder, J., Samuels, G. J., Summerell, B. A., Thrane, U.and Waalwijk, C. 2003. The name Fusarium moniliforme should no longer be used. Mycological Research 107(6):643-644.   DOI   ScienceOn
23 Torres, O. A., Palencia, E., Pratdesaba, L. L., Grajeda, R.,Fuentes, M., Speer, M. C., Merrill Jr., A. H., O'Donnell, K. Bacon, C. W., Glenn, A. E. and Riley, R. T. 2007. Estimated fumonisin exposure in Guatemala is greatest in consumers of lowland maize. The Journal of Nutrition 137:2723-2729.   DOI
24 Jardine, D. J. and Leslie, J. F. 1999. Aggressiveness to mature maize plants of Fusarium strains differing in ability to produce fumonisin. Plant Disease 83(7):690-693   DOI
25 김완규, 오인석, 유승헌, 박종성. 1984.옥수수 종자에서 검출된 Fusarium moniliforme와 그 병리학적 중요성. 한국균학회지 12(3):105-110.   과학기술학회마을
26 김종군, 최재을, 강희완. 2007. 국내 포도나무 혹병(Agrobacterium vitis) 균주의 유전적 다양성. 식물병연구 13(3):137-144.   과학기술학회마을   DOI
27 농업생명공학연구원. 2006. 유전자원 특성조사 및 관리요령. 농촌진흥청. 3-8.
28 이인원, 강효중. 1994. 중국산 옥수수로부터 분리한 Fusarium균주의 독성 및 Fumonisin $B_{1}$의 생성. 한국식물병리학회지 10(2):129-135.   과학기술학회마을
29 한국식물병리학회. 2004. 한국식물병목록 제4판 45-47.
30 민병례, 양연주, 최영길. 1999. Fusarium 종에서의 RAPD-PCR분석. The Korean Journal of Microbiology 35(2):107-114.   과학기술학회마을
31 Fandohan, P., Hell, K., Marasas, W. F. O. and Wingfield, M. J.2003. Infection of maize by Fusarium species and contamination with fumonisin in africa. African Journal of biotechnology 2(12):570-579.   DOI
32 Geiser, D. M., Jimnez-Gasco, M. M., Kang, S., Makalowska, I.,Veeraraghavan, N., Ward, T. J., Zhang, N., Kuldau, G. A. and O'Donnell, K. 2004. Fusarium-ID v. 1.0: A DNA sequence database for identifying Fusarium. European Journal of Plant Pathology 110:473-479.   DOI   ScienceOn
33 Nirenberg, H. and O'Donnell, K. 1998. New Fusarium species and combinations within the Gibberella fujikuroi species complex. Mycologia 90:434-458.   DOI   ScienceOn
34 O'Donnell, K., Cigelnik, E. and Nirenberg, H. 1998a. Molecular systematics and phylogeography of the Gibberella fujikuroi species complex. Mycologia 90:465-493.   DOI   ScienceOn
35 Amoah, B. K., Macdonald, M. V., Rezanoor, N. and Nicholson, P. 1996. The use of the random amplified polymorphic DNA technique to identify mating groups in the Fusarium section Liseola. Plant Pathology 45:115-125.   DOI
36 Fisher, N. L., Burgess, L. W., Toussoun, T. A. and Nelson, P. E.1982. Carnation leaves as a substrate and for preserving cultures of Fusarium species. Phytopathology 72(1):151-153.   DOI