• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.21-21
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• 2001

일반적으로 세포는 방사능이나 항암제 등의 자극에 의해 DNA가 손상받았을 때 DNA를 합성하기 전, DNA변이를 복구하기 위해 cell cycle을 정지시키게 된다. pRB(retinoblatoma protein)는 이러한 cell cycle의 조절기작에서 중요한 역할을 담당하는 것으로 알려져 있다. G1기에서 S기로 진행하는 것을 조절하는 단백질인 pRB 은 E2F(cell cycle transcription factor)와 상호작용하여 cell cycle 진행에 필요한 전사활성을 억제, PCNA (proliferating cell nuclear antigen)의 합성을 저해한다. 또한, E2F와 결합된 pRB는 apoptosis를 제어하는 유전자를 조절하는 것으로 알려져 있다. Cell cycle에 영향을 미치는 항암제의 일종인 busulfan을 처리하면, 정소 내에 존재하는 대부분의 생식세포들은 사멸되고 spermatogonia만 남는 것으로 알려져 있다. 그러나 그 기작에 대해서는 자세히 연구된 바가 없다. 본 연구에서는 busulfan처리시 spermatogonial stem cell이 어떤 기작에 의해 손상받지 않고 유지되는지를 알아보고자 실험을 수행하였다. Busulfan을 처리한 마우스 (항암제 투여 후 5주)와 정상적인 13주령의 마우스의 정소로부터 각각 세포를 분리하였다. LSC (laser scanning cytometry)를 이용하여 처리군(busulfan treated mice)과 대조군(normal mature mice)에 대해 각각 DNA함량을 비교ㆍ분석한 결과 G0/G1(2N)에 머물러 있는 세포비율이 처리군에서 현저하게 증가했다 (79.3$\pm$5.5%:8.1$\pm$1.3%). Cell cycle의 G1/S check point인 pRB와 PCNA 발현을 Western blot과 면역조직학적인 방법(immunohisto-chemistry)을 이용하여 조사하였다 PCNA는 대조군과 비교해, 처리군에서 매우 낮은 수준으로 발현되었다. 면역염색된 정소단면을 살펴보면, 대조군에서는 모든 세정관에서 PCNA를 발현하는 세포가 높은 비율로 검출되었고, 처리군에서는 소수의 세정관에서 세포들이 낮은 수준으로 검출되었다. 반면에, pRB의 경우 PCNA와는 상반된 결과를 나타내어, 대조군에서는 거의 발현이 되지 않는 반면, 처리군에서는 대부분의 세정관내, 기저막을 따라 위치한 세포들에서 발현되었다. 이상의 결과는 busulfan에 의해 pRB의 인산화가 억제, pRB 와 결합된 E2F는 전사 활성이 억제되어, PCNA 합성을 저해하는 것으로 설명되어질 수 있다. 결론적으로, 인산화가 억제된 pRB (underphosphorylated RB protein)이 quiescent spermatogonial stem cell에서만 특이하게 발현하는 단백질이며, 이러한 pRB의 발현은 apoptosis를 제어하는 역할을 담당해 busulfan처리에 의해 손상받지 않고 남아있는 것으로 시사된다.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2003.05c
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• pp.91-94
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• 2003

The purpose of this project is to research and development of thin film supercapacitor with conducting polymer composite electrodes and polymer electrolyte which have high energy density for thin film supercapacitor. We investigated cyclic voltammetry and charge/discharge cycling of PFPT-flyash electrodes. The first discharge capacity of PFPT-flyash electrode with 40wt.% flyash was 24F/g, while that of PFPT-VOflyash electrode with 40wt.% VOflyash was 32F/g. The capacitance of PFPT-VOflyash composite film with polymer electrolyte was 32 F/g at 1st and 20th cycle, respectively. The capacitance of PFPT-VOflyash/Li cell with 40 wt% VOflyash was 141 F/g at 8th cycle.

• Journal of Ocean Engineering and Technology
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• v.3 no.2
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• pp.617-617
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• 1989

This paper deals with a fatigue life prediction of a surface crack based on the experimentally obtained relationship between surface crack length ratio $a/a_{f}$ and cycle ratio $N/N_{f}$ using micro computer. Firstly $a/a_{f}$-$N/N_{f}$ curves obtained from experimental tests, were assumed as three curves UC(the upper limit curve), LC(the lower limit curve) and MC(the middle curve), and these were utilized to predict the fatigue life. Comparing the calculated values which represent the characteristics of crack growth behaviors from the three assumed curves with the experimental ones, it has been found that in the stable crack growth region, they coincide reasonably well each other. And the differences between the fatigue lives obtained from the assumed curves and the experimental fatigue life did not exceed 20%. Using the characteristics of $a/a_{f}$-$N/N_{f}$ curves, it is possible to predict the da/dN-Kmax curves and the S-$N_{f}$ curves.

• Journal of Ocean Engineering and Technology
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• v.3 no.2
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• pp.108-117
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• 1989

This paper deals with a fatigue life prediction of a surface crack based on the experimentally obtained relationship between surface crack length ratio $a/a_{f}$ and cycle ratio $N/N_{f}$ using micro computer. Firstly $a/a_{f}$-$N/N_{f}$ curves obtained from experimental tests, were assumed as three curves UC(the upper limit curve), LC(the lower limit curve) and MC(the middle curve), and these were utilized to predict the fatigue life. Comparing the calculated values which represent the characteristics of crack growth behaviors from the three assumed curves with the experimental ones, it has been found that in the stable crack growth region, they coincide reasonably well each other. And the differences between the fatigue lives obtained from the assumed curves and the experimental fatigue life did not exceed 20%. Using the characteristics of $a/a_{f}$-$N/N_{f}$ curves, it is possible to predict the da/dN-Kmax curves and the S-$N_{f}$ curves.

• Korean Journal of Animal Reproduction
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• v.5 no.1
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• pp.16-25
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• 1981

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2012.05a
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• pp.52-57
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• 2012

A liquid rocket system consists of a combustion chamber, a gas generator, a turbo pump, and a turbine, etc. Each component is connected by supply components such as valves, pipes, and orifices. Since each component has a combined effect on engine performance, preliminary analysis for overall system must be required before the conceptual design stage. Comprehensive analysis program considered the supply system has not been developed yet. In this paper, a supply component model of the liquid rocket engine has been designed after verification of each component. The gas generator cycle with supply components has been composed. The results of the cycle has been compared to those of the F-1 engine with the representative gas generator cycle.

• Biomolecules & Therapeutics
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• v.10 no.4
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• pp.293-302
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• 2002

The classical concept for iron uptake into mammalian cells has been the endocytosis of transferrin( $T_{f}$ )-bound F $e^{3+}$ via the $T_{f}$ - $T_{f}$ receptor cycle. In this case, we could not explain the uptake of F $e^{2＋}$ ion and the export of iron from endosome. Studies on iron transport revealed that other transport system exists in epithelial cells of the intestine. One of non- $T_{f}$ -receptor-mediated transport systems is Nramp2/DMT1/DCT1 which transports M $n^{＋＋}$, $Mg^{＋＋}$, Z $n^{＋＋}$, $Co^{＋＋}$, N $i^{＋＋}$ or C $u^{＋＋}$ ion as well as F $e^{+2}$ ion. DMT1 was cloned from intestines of iron-deficient rats and shown to be a hydrogen ion-coupled iron transporter and a protein regulated by absorbed dietary iron. DMT1 is founded in other cells such as cortical and hippocampal glial cells as well as endothelial cells in duodenum. Two F $e^{3+}$ ion bound to transferrin( $T_{f}$ ) are taken up via the $T_{f}$ - $T_{f}$ receptor cycle in the intestinal epithelial cell. F $e^{3+}$ in endosome was converted to F $e^{2＋}$ ion, and then exported to cytosol via DMT1. F $e^{2＋}$ ion is taken up into cytosol via DMT1. Several other transporters such as FET, FRE, CCC2, AFT1, SMF, FTR, ZER, ZIP, ZnT and CTR have been reported recently and dysfunction of the transporters are related with diseases containing Wilson's disease, Menkes disease and hemochromatosis. Evidences from several studies strongly suggest that DMT1 is the major transporter of iron in the intestine and functions critically in transport of other metal ions.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.25 no.1
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• pp.39-46
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• 1980

A male sterile facilitated recurrent selection population was developed by the barley breeding team of Yeongnam Crop Experiment Station. To breed this population, three composite cross populations and a composite population of breeding lines in our country were used as the materials. This population was developed in order to breed early maturing, wet-soil tolerant and high yielding barley varieties, adapted for double cropping with rice in southern part of Korea. The population was named by RSPYB#l which means Recurrent Selection Population No.1. of Yeongnam Barley.

• Journal of The Korean Society of Agricultural Engineers
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• v.57 no.4
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• pp.143-151
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• 2015

Recently, flood has been increased due to climate change resulting in numerous damages for humans and properties. The main objective of this study was to suggest a methodology to estimate the flood vulnerability using Potential Flood Damage (PFD) concept. To evaluate the PFD at a spatial resolutions of city/county units, the 19 representative evaluation indexing factors were carefully selected for the three categories such as damage target ($F_{DT}$), damage potential ($F_{DP}$) and prevention ability ($F_{PA}$). The three flood vulnerability indices of $F_{DT}$, $F_{DP}$ and $F_{PA}$ were applied for the 162 cities and counties in Korea for the pattern classification of potential flood damage. It is expected that the supposed PFD can be utilized as the useful flood vulnerability index for more rational and practical protection plans against flood damage.

• Transactions of the Korean Society of Mechanical Engineers
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• v.12 no.5
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• pp.1097-1103
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• 1988

This paper deals with surface crack behavior and the fatigue life prediction of notched specimens using the relation between surface crack length, a, and the cycle ratio, $N/N_{f}$. From the $a-N\;/\;N_{f}$ curves, UC(the upper limit curve), LC(the lower limit curve) and MC(the middle limit curve) were assumed and utilized to predict the fatigue life and crack growth rate. The data computed from the three assumed curves were compared with the experimental data. It has been found that in the stable crack growth region ($N/N_{f}=0.3-0.8$) fatigue life can be predicted within 20% errors. Using the characteristics of $a-N\;/\;N_{f}$ curve, it is possible to predict the $da/dN-K_{max}$ curve, the $da/dN-{\Delta}K_{{\varepsilon}_t}$ curve, and the $S-N_{f}$ curve.