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Glycogen Metabolism in Vibrio vulnificus Affected by malP and malQ

  • Han, Ah-Reum;Lee, Yeon-Ju;Wang, Tianshi;Kim, Jung-Wan
    • Microbiology and Biotechnology Letters
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    • v.46 no.1
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    • pp.29-39
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    • 2018
  • Vibrio vulnificus needs various responsive mechanisms to survive and transmit successfully in alternative niches of human and marine environments, and to ensure the acquisition of steady energy supply to facilitate such unique life style. The bacterium had genetic constitution very different from that of Escherichia coli regarding metabolism of glycogen, a major energy reserve. V. vulnificus accumulated more glycogen than other bacteria and at various levels according to culture medium and carbon source supplied in excess. Glycogen was accumulated to the highest level in Luria-Bertani (3.08 mg/mg protein) and heart infusion (4.30 mg/mg protein) complex media supplemented with 1% (w/v) maltodextrin at 3 h into the stationary phase. Regarding effect of carbon source, more glycogen was accumulated when maltodextrin (2.34 mg/mg protein) was added than when glucose or maltose (0.78.1-14 mg/mg protein) was added as an excessive carbon source to M9 minimal medium, suggesting that maltodextrin metabolism might affect glycogen metabolism very closely. These results were supported by the analysis using the malP (encoding a maltodextrin phosphorylase) and malQ (encoding a 4-${\alpha}$-glucanotransferase) mutants, which accumulated much less glycogen than wild type when either glucose or maltodextrin was supplied as an excessive carbon source, but at different levels (3.1-80.3% of wild type glycogen). Therefore, multiple pathways for glycogen metabolism were likely to function in V. vulnificus and that responding to maltodextrin might be more efficient in synthesizing glycogen. All of the glycogen samples from 3 V. vulnificus strains under various conditions showed a narrow side chain length distribution with short chains (G4-G6) as major ones. Not only the comparatively large accumulation volume but also the structure of glycogen in V. vulnificus, compared to other bacteria, may explain durability of the bacterium in external environment.

Numerical investigation of an MR damper-based smart passive control system for mitigating vibration of stay cables

  • Kim, In-Ho;Jung, Hyung-Jo;Kim, Jeong-Tae
    • Structural Engineering and Mechanics
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    • v.37 no.4
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    • pp.443-458
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    • 2011
  • An extensive numerical investigation on the magnetorheological (MR) damper-based smart passive control system for mitigating vibration of stay cables under wind loads has been conducted. The smart passive system is incorporated with an electromagnetic induction (EMI) device for reducing complexity of the conventional MR damper based semi-active control system by eliminating an external power supply part and a feedback control part (i.e., sensors and controller). In this study, the control performance of the smart passive system has been evaluated by using a cable structure model extracted from a full-scale long stay cable with high tension. Numerical simulation results of the proposed smart damping system are compared with those of the passive and semi-active control systems employing MR dampers. It is demonstrated from the results that the control performance of the smart passive control system is better than those of the passive control cases and comparable to those of the semi-active control systems in the forced vibration analysis as well as the free vibration analysis, even though there is no external power source in the smart passive system.

Analysis of flow rate of variable displacement compressor ECV in automobile air conditioning control system (자동차 공조장치의 가변압축기 ECV 유량 분석)

  • Jeong, Yeong Jun;Cho, Haeng Muk
    • Journal of Energy Engineering
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    • v.22 no.4
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    • pp.394-398
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    • 2013
  • At present, using of electromagnetic control valve (ECV) in external variable displacement swash plate type compressor is a common issue. Solenoid operated ECV controls vehicle air conditioning system through a pulse width modulation (PWM) input signal that supplied from an external source. Different port pressure controls the swash plate movement inside the compressor at certain angles that finally determines the refrigerant flow rate to the vehicle compartment for passengers comfort. In this paper, crankcase pressure flow (Pc flow) is calculated with some ECV samples with corresponding to amount of variable supply of current ranges from 0.20Amp to 0.95Amp.

Rectal Injuries after Radiotherapy for Carcinoma of the Uterine Cervix (자궁경암(子宮頸癌) 방사선치료(放射線治療)에 의한 직장손상(直腸損傷))

  • Kim, Jung Jin
    • Radiation Oncology Journal
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    • v.1 no.1
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    • pp.103-109
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    • 1983
  • 47 out of 56 cases of intact uterine cervix cancer treated by radiation at the Hanyang University Hospital were followed 18 months or more after treatment. (7 patients died before 18 months, 2 cases lost to follow-up). Age distribution reveal 5 cases in 30's, 18 cases in 40's, 17 cases in 50's, 7 cases in 60's. Histologically, all cases were squamous cell type except one case of adenocarcinoma. 1. 45 cases were treated by combined external Co-60 irradiation and intracavitary irradiation by Cs-137 small sources. 1 case was treated by external irradiation only, and 1 case by intracavitary only. 2. Rectal injuries were observed in 13 cased (27.6%), 4 cases in Grade 1, 8 cased in Grade 2 and 1 cases in Grade 3 which needed surgical management. 3. Average intervals of rectal injury following treatment was 9.2 months varying from 5 to 15 months. 4. Relation between rectal injury and point A dose reveal 6 cases between 7000-7999 rad and 6 cases between 8000-8999 rad and 1 case above 9000 rad. Even though there is no direct relation between point A dose and rectal injury, it is expected that rectal injury increases as point A dose increase. 5. In the normal condition, rectal injury can't be attributed to one major cause. Radiation dose, small source distribution, general condition of patients, local anatomy of the individual patient, history of PID and previous surgery, all play complex roles.

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A Study on the Symbolic Meaning and Influence of Punk Style (펑크 스타일의 상징적 의미와 영향에 관한 연구)

  • 김순자
    • Journal of the Korean Society of Costume
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    • v.52 no.6
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    • pp.139-164
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    • 2002
  • The objective of this Paper was to identify the internal and external symbols of Punks, and to assess the significance of punk style giving an inspiration to many high fashion designers. For this objective. comprehension on widely discussed idea of punk, furthermore grasping the internal and external form by inquiring historically punk look, and importantly looking into new ideas pursued by punk fashion by studying traits of punk image in 1980's and after are done. Punk in the latter half of the 70's was anti-fashion expressed for the young of working class to rebel against the established generation. At first some of the fashion was popularized by the young who imitated pop stars under the specific economy of Britain, and others with movement of hippie Punk is aesthetics extremism which neglects traditionalism in the past but pursues newness. It denied traditional way of expression and principles of aesthetics but created newness. Punk look was symbolized as poor look. grotesque, androgynous, black, graffiti, and bricolage. And the internal meanings of these symbols are ego-screaming, expression of nihilism, resistance, irony and ridicule, search for hedonism. The punk phenomenon continues today. Some teens still cling to the complete look. whether they have punks' idea and value or not. Diverse elements of punk style play a major role in the current trend of fashion and a source of the fresh inspiration for the high fashion designers. Punk style is truly the story of a fad that turned to fashion.

Study on Timing Characteristics of High-Voltage Pulse Generation with Different Charging Voltages

  • Lee, Ki Wook;Kim, Jung Ho;Oh, Sungsup;Lee, Wangyong;Kim, Woo-Joong;Yoon, Young Joong
    • Journal of electromagnetic engineering and science
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    • v.18 no.1
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    • pp.20-28
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    • 2018
  • The time synchronization of each sub-unit of a pulsed generator is important to generate an output high-power radio frequency (RF) signal. To obtain the time synchronization between an input RF signal fed by an external source and an electron beam produced by an electric pulse generator, the influence of different charging voltages on a delay and a rise time of the output pulse waveform in the electric pulse generator should be carefully considered. This paper aims to study the timing characteristics of the delay and the rise time as a function of different charging voltages with a peak value of less than -35 kV in the high-voltage pulse generator, including a trigger generator (TG) and a pulse-forming line (PFL). The simulation has been carried out to estimate characteristics in the time domain, in addition to their output high-voltage amplitude. Experimental results compared with those obtained by simulation indicate that the delay of the output pulses of the TG and PFL, which are made by controlling the external triggering signal with respect to different charging voltages, is getting longer as the charging voltage is increasing, and their rise times are inversely proportional to the amplitude of the charging voltage.

The Study of an Automatic Tracking and Pointing Method and the Regarding System for Facing Two Antennas (상호 대국의 안테나 간 자동 추적 지향 기법 및 장치 연구)

  • Gimm, Hak In;Cho, Sung Hoon;Lee, Chong Hyo
    • Journal of the Korea Institute of Military Science and Technology
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    • v.18 no.5
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    • pp.498-509
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    • 2015
  • The existing mobile antenna networks in the military use have been operated by the manual pointing between two antennas. The work presented here describes the study of ATPC(Automatic Tracking and Pointing Control) system between facing antennas and the related tracking and pointing performances. This system is able to automatically track the maximum RSSI(Received Signal Strength Indication) value from the source's RF(Radio Frequency) signal and then control for maintaining the LOS(Line of Sight) between two antennas. The system has three major units; the driving unit consisting of motors, harmonic drives and encoders, the sensor unit with a GPS(Global Positioning System) and AHRS(Attitude and Heading Reference System) and the control unit regulating all the tracking and pointing events. By using PI(Proportional and Integral) controller, this system is able to properly track and point the other antenna under the external disturbance like the wind load. Both the simulation and the experimental works have been successively carried out to prove the performances of the system.

A Study on the Enhancement of Cooling Efficiency for the Cabinet of Automatic Controller in the Interior of Industrial Building (산업용 건축물 내 자동제어반의 냉각효율 향상에 관한 연구)

  • Kim, Soon-Ho;Park, Hyun-Jung
    • Journal of Power System Engineering
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    • v.17 no.6
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    • pp.79-87
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    • 2013
  • The improvement of cooling efficiency for the cabinet of automatic controller is the most efficient method of it's application. Therefore, this study has been analyzed and investigated the improvement of cooling efficiency and reduction of energy for the cabinet of automatic controller, respectively. So this study was conducted to enhancement of cooling efficiency for the cabinet of automatic controller by making a structure which produces difference of air pressures in the entrance tube of external air. And the structure has capacity of the pyrogen source (PTC elements) to make temperature range from $145^{\circ}C$ to $155^{\circ}C$. Consequently, temperatures of the upper, the lower in the interior of the cabinet of automatic controller and the exhaust part were revealed $28.57^{\circ}C$, $23.38^{\circ}C$and $36.14^{\circ}C$(average temperature of the exhaust part in case of existing method : $45^{\circ}C$) in target test of this study, respectively. It was found that the cabinet of the automatic controller has better cooling ability than the cabinet of automatic controller by using an existing method.

Fast Defect Detection of PCB using Ultrasound Thermography (초음파 서모그라피를 이용한 빠른 PCB 결함 검출)

  • Cho, Jai-Wan;Jung, Hyun-Kyu;Seo, Yong-Chil;Jung, Seung-Ho;Kim, Seung-Ho
    • Proceedings of the KIEE Conference
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    • 2005.10b
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    • pp.273-275
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    • 2005
  • Active thermography is being used since several years for remote non-destructive testing. It provides thermal images for remote detection and imaging of damages. Also, it is based on propagation and reflection of thermal waves which are launched from the surface into the inspected component by absorption of modulated radiation. For energy deposition, it use external heat sources (e.g., halogen lamp or convective heating) or internal heat generation (e.g., microwaves, eddy current, or elastic wave). Among the external heat sources, the ultrasound is generally used for energy deposition because of defect selective heating up. The heat source generating a thermal wave is provided by the defect itself due to the attenuation of amplitude modulated ultrasound. A defect causes locally enhanced losses and consequently selective heating up. Therefore amplitude modulation of the injected ultrasonic wave turns a defect into a thermal wave transmitter whose signal is detected at the surface by thermal infrared camera. This way ultrasound thermography(UT) allows for selective defect detection which enhances the probability of defect detection in the presence of complicated intact structures. In this paper the applicability of UT for fast defect detection is described. Examples are presented showing the detection of defects in PCB material. Measurements were performed on various kinds of typical defects in PCB materials (both Cu metal and non-metal epoxy). The obtained thermal image reveals area of defect in row of thick epoxy material and PCB.

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Optimal Design of Filament Wound Composite Cylinders under External Hydrostatic Pressure using a Micro-Genetic Algorithm (마이크로 유전자 알고리즘을 이용한 외부 수압을 받는 필라멘트 와인딩 복합재 원통의 최적 설계)

  • Moon, Chul-Jin;Kweon, Jin-Hwe;Choi, Jin-Ho
    • Composites Research
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    • v.23 no.4
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    • pp.14-20
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    • 2010
  • In this study, a micro-genetic algorithm was utilized for the optimal design of filament wound composite cylinders subjected to hydrostatic pressure for underwater vehicle application. The objective of the optimization was to maximize the design allowable load considering the buckling and static failure loads. A commercial finite element program, MSC.NASTRAN, was used for buckling and failure analysis. An open-source micro genetic algorithm by Carroll was modified for the optimization. The design variables are the helical winding angle and hoop layer thickness. The results of examples show that the micro genetic algorithm can be successfully applied to the optimization of filament wound cylinders with various geometries and gives better efficiency than general genetic algorithms.