• 생명과학회지
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• 제24권2호
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• pp.186-190
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• 2014

본 논문에서는 삼세기(Shaggy sea raven, Hemitripterus villosus)를 실험 재료로 선택하여 Carbonic Anhydrase Isozymes (CAs) 중의 하나인 CA III에 대한 연구를 SDS-PAGE, Isoelectric Focusing (IEF), Western blot analysis의 방법을 통하여 진행하였다. SDS-PAGE와 Western blot 결과 삼세기 아가미, 혈액, 장, 간, 신당, 근육, 심장조직에서 CA III의 분자량인 30 kDa의 band가 확인되었다. 삼세기의 근육과 아가미에 대한 등전점 전기영동(IEF)과 Western blot analysis 결과 pI 7.0 부근에서 band가 형성되는 것을 확인할 수 있었다. 특히 SDS-PAGE와 IEF 실험결과 삼세기의 아가미 조직에서 CA III의 발현량이 다른 조직들에 비하여 우세하게 나타났다. 이는 아가미가 다른 조직들과 달리 어류의 생체기관 중 유일하게 외부와 직접 접촉이 가능한 조직으로서 활성산소에 대한 손상을 최소화하기 위한 것으로 사료된다.

• 에너지공학
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• 제26권2호
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• pp.73-79
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• 2017

In nucleation assisted crystallization process formed $CO_2$ leaves as colloid gas and is used as the template by the rapidly growing crystals in the nucleation site. This emulsion of $CaCO_3$ micro-crystals & $CO_2$ micro-bubbles forms hollow particles. Formed hollow particles are double walled, both internal and external faces belonging to the cleavage aragonites which separate the surrounding water from the enclosed gas cavity. Hence, the reverse reaction of $CO_2$ with water forming Carbonic Acid is not possible and the pH stability is maintained. In fact every excess $CaCO_3$ crystals are buffering any carbonic acid left over. This $CO_2$ based nucleation technology prevents scale formation in water channels, but it also helps to reduce the previously formed scales. This process takes out water dissolved $CO_2$ in almost-visible micro-bubbles forms that helps reducing previously formed scale over a period of time (depends on the usage period). The aragonite crystals can't form scale because of its stable molecular structure and neutral surface electro potentiality.

• Progress in Superconductivity
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• 제7권1호
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• pp.17-21
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• 2005

We report the oscillation of the Josephson vortex-flow resistance in the rectangular stacks of $Bi_{2}Sr_{2}CaCu_{2}O_{8+x}$(Bi-2212) intrinsic Josephson junctions (IJJs). Apiece of Bi-2212 single crystal containing a few tens of IJJs was sandwiched between two gold electrodes and fabricated into a rectangular shape with the typical lateral size of about $1.5{\times}10\;{\mu}m^2$, using e-beam lithography and focused ion-beam etching techniques. In a tesla-range magnetic field applied in parallel with the junction planes, the oscillation of the Josephson vortex flow resistance was observed at temperatures near 60 K. The oscillation results from the interplay between the triangular Josephson vortex lattice and the potential barrier at the boundary of a single crystal. The oscillatory magnetoresistance for different bias currents, external magnetic fields, and the tilt-angles provides useful information on the dynamics of the coupled Josephson-vortex lattice system.

• 한국수소및신에너지학회논문집
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• 제20권5호
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• pp.404-409
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• 2009

The present works were performed that titanium foil was anodized in various dilution ratios of seawater and distilled water with 10V external voltage applied, then annealed at $450^{\circ}C$ to obtain $TiO_2$ on the Ti substrate. The prepared samples were characterized by instruments (XRD, SEM, and photocurrent) and used to investigate rate of hydrogen production in photoelectrochemical cell as well as Cr(VI) reduction. As the results of experiments, the anodized $TiO_2$ in seawater electrolytes, which are ranged from 15 to 50 times dilution of seawater, was showed a relatively higher hydrogen production (ca. 97~110 umol/hr-$cm^2$) and Cr(VI) reduction (ca. 95% reduction).

• 상하수도학회지
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• 제22권6호
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• pp.641-646
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• 2008

In this research, recovery of high quality organics from excess activated sludge and its potential as a external carbon sources for BNR process was studied. By simultaneous treatment of alkali and ozone, TSS concentration was reduced by 32%, and RBDCOD fraction was increased by 76.2%, and major constitute of produced organic were acetic acid and propionic acid. Also, nitrogen and phosphorus were greatly solubilized. However, because acid-hydrolyzable phosphorus(AHP) was major part of solubilized phosphorus, $NH_4{^+}-N$ and $PO_4{^3}-P$ concentration were insufficient for effective formation of crystal like as MAP(Magnesium Ammonium Phosphate) and hydroxyapatite. By placing BPR reactor before alkali-ozone treatment reactor, $PO_4{^3}-P$ concentration in pretreated sludge was increased by 1.8 times, and improved potential of phosphorus recovery by crystallization. In experiment of crystallization, hydroxyapatite formation was more easily applied than MAP. By hydroxyapatite formation, $SCOD/PO_4-P$ ratio was greatly increased from 32.7 at control to 141.9 at $Ca^{2+}/PO{_4}^{3-}-P$ mole ratio of 2.4. The results based on this study indicated that the proposed system configuration has potential to reduce the excess sludge production, to recover phosphorus in usable forms as well as utilize organics as a external carbon source in BNR process.

• ALGAE
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• 제31권3호
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• pp.243-256
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• 2016

Concerns about how ocean acidification will impact marine organisms have steadily increased in recent years, but there is a lack of knowledge on the responses of macroalgae. Here, we adopt an outdoor continuous-flowing mesocosm system designed for ocean acidification experiment that allows high CO₂ conditions to vary with natural fluctuations in the environment. Following the establishment of the mesocosm, five species of macroalgae that are common along the coast of Korea (namely Ulva pertusa, Codium fragile, Sargassum thunbergii, S. horneri, and Prionitis cornea) were exposed to three different CO₂ concentrations: ambient (×1) and elevated CO₂ (2× and 4× ambient), over two-week period, and their ecophysiological traits were measured. Results indicated that both photosynthesis and growth exhibited species-specific responses to the different CO₂ concentrations. Most notably, photosynthesis and growth increased in S. thunbergii when exposed to elevated CO₂ conditions but decreased in P. cornea. The preference for different inorganic carbon species (CO₂ and HCO₃⁻), which were estimated by gross photosynthesis in the presence and absence of the external carbonic anhydrase (eCA) inhibitor acetazolamide, were also found to vary among species and CO₂ treatments. Specifically, the two Sargassum species exhibited decreased eCA inhibition of photosynthesis with increased growth when exposed to high CO₂ conditions. In contrast, growth of U. pertusa and C. fragile were not notably affected by increased CO₂. Together, these results suggest that the five species of macroalgae may respond differently to changes in ocean acidity, with species-specific responses based on their differentiated photosynthetic acclimation. Understanding these physiological changes might allow us to better predict future changes in macroalgal communities in a more acidic ocean.

• 대한환경공학회지
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• 제34권3호
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• pp.155-161
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• 2012

본 연구에서는 고순도 메탄을 회수하기 위해서 Plug Flow Reactor와 External $CO_2$ Stripper를 결합한 중온 Methane Enhancement System을 개발하였다. 반응조 운전인자로서 알칼리도와 Leachate 순환율(LRR, Leachate Recycle Rate)이 바이오가스의 조성과 생성량 및 TVS 제거효율에 미치는 영향을 규명하였다. 고순도 메탄회수 공정 운전결과 OLR 2 g TVS/L-d, 알칼리도 4 g/L as $CaCO_3$, Leachate 순환율 3 v/v-d일 때 평균 94%의 높은 메탄함량을 나타내 고순도 메탄회수를 위한 최적조건임이 밝혀졌다. 이때 1일 반응조 단위 부피당 0.71부피의 메탄이 생성되었으며, TVS 제거율은 79%로서 Control Reactor의 94% 수준을 달성하였다.

• 한국미생물·생명공학회지
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• 제16권6호
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• pp.510-516
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• 1988

본 연구는 균주로 Rhodospirillum rubrum KS-301, 증식 제한 기질로 글루코오스, 고정화 담체로 Ca alginate를 사용한 고정화 생물 반응기를 조작할 때 담체에 의해 형성되는 물질전달 저항에 대한 도입 기질의 농도, 희석속도의 영향을 고찰하였다. 또한 효율인자를 평가하기 위해 속도식 변수를 구하였다. 충전층 반응기의 경우 외부 액막 물질전달 저항보다는 내부 물질전달 저항이 우세함을 알 수 있었고 총괄 효율인자는 희석속도의 증가에 따라 감소하였다. 연속 교반 탱크 반응기의 경우 외부 액막 물질전달 저항은 무시할 수 있으며 총괄 효율인자는 희석속도에 영향을 받지 않았다. 희석속도 0.2/h, 비드 반경 0.15cm, 초기 글루코오스 농도 1.0g/L의 실험조건에서 총괄 효율인자는 충전층 반응기와 연속 교반 반응기에서 각각 0.70과 0.77이었다.

• Molecules and Cells
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• 제43권8호
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• pp.671-685
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• 2020

The regulator of calcineurin (RCAN) was first reported as a novel gene called DSCR1, encoded in a region termed the Down syndrome critical region (DSCR) of human chromosome 21. Genome sequence comparisons across species using bioinformatics revealed three members of the RCAN gene family, RCAN1, RCAN2, and RCAN3, present in most jawed vertebrates, with one member observed in most invertebrates and fungi. RCAN is most highly expressed in brain and striated muscles, but expression has been reported in many other tissues, as well, including the heart and kidneys. Expression levels of RCAN homologs are responsive to external stressors such as reactive oxygen species, Ca²⁺, amyloid β, and hormonal changes and upregulated in pathological conditions, including Alzheimer's disease, cardiac hypertrophy, diabetes, and degenerative neuropathy. RCAN binding to calcineurin, a Ca²⁺/calmodulin-dependent phosphatase, inhibits calcineurin activity, thereby regulating different physiological events via dephosphorylation of important substrates. Novel functions of RCANs have recently emerged, indicating involvement in mitochondria homeostasis, RNA binding, circadian rhythms, obesity, and thermogenesis, some of which are calcineurin-independent. These developments suggest that besides significant contributions to DS pathologies and calcineurin regulation, RCAN is an important participant across physiological systems, suggesting it as a favorable therapeutic target.

• The Korean Journal of Physiology
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• 제14권2호
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• pp.7-16
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• 1980

The effects of various inotropic interventions on the shape of the steady state length tension relation and the length-dependent activation process in cardiac muscle were studied. The influence of inotropic interventions upon the action potential was also observed. The range of varying muscle length was from the optimal length$(l_{max})$, where the active tension production is maximal, to 0.85 $l_{max}$. Changes in stimulus frequency or in external bathing Ca concentration constituted the inotropic interventions in this experiment. The papillary muscles were isolated from the rabbit right ventricles and perfused with $HCO-_3\;-buffered$ normal Tyrode solution which was aerated with $3%\;CO_2-97%\;O_2$ mixed gas and kept at $35^{\circ}C$. Resting Passive tension at $l_{max}$ was approximately 30% of the total tension and appeared from the muscle length of 0.90 $l_{max}$. The effect of stimulus frequency on the steady state level of developed tension was: As the stimulus frequency was increased from 0.1 to 0.5 Hz, there was little change in developed tension. As the frequency was increased further, to a value of about 3 Hz, tension increased steeply. Further increase of the frequency to 5 Hz had little additional effect on the developed tension. The length-tension curves for isometric peak tension became more steeper with the degree of potentiation by inotropic interventions. The relative steepness of the normalized length-tension curves where tension production was expressed as a percentage of maximal tension developed at $l_{max}$, varied inversely with the level of inotropic state and these curves were not superimposable one another. Thus at the stimulus frequency of 2 Hz or at the external Ca concentration of 8 mM, the relative decline in the developed tension for a given change in muscle length was considerably less than the decline observed at the frequency of 0.5 Hz or at the concentration of 2 mM Ca. Action potential duration was prolonged significantly as the frequency increased from 0.2 to 2 Hz, and this change in action potential duration was not observable on the changes in muscle length. There was a tendency of the hyperpolarization of membrane potential when the muscle length was shortened from $l_{max}$ to 0.95 $l_{max}$. These results support the hypothesis that there is a length-dependence of the activation process.