• Parasites, Hosts and Diseases
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• v.51 no.2
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• pp.247-253
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• 2013

Neospora caninum is the etiologic agent of bovine neosporosis, which affects the reproductive performance of cattle worldwide. The transmembrane protein, NcSRS2, and dense-granule protein, NcGRA7, were identified as protective antigens based on their ability to induce significant protective immune responses in murine neosporosis models. In the current study, NcSRS2 and NcGRA7 genes were spliced by overlap-extension PCR in a recombinant adenovirus termed Ad5-NcSRS2-NcGRA 7, expressing the NcSRS2-NcGRA7 gene, and the efficacy was evaluated in mice. The results showed that the titer of the recombinant adenovirus was $10^9TCID_{50}/ml$. Three weeks post-boost immunization (w.p.b.i.), the IgG antibody titer in sera was as high as 1:4,096. IFN-${\gamma}$ and IL-4 levels were significantly different from the control group (P<0.01). This research established a solid foundation for the development of a recombinant adenovirus vaccine against bovine N. caninum.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.23 no.3
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• pp.133-141
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• 2017

This article described the development of transparent antimicrobial oxo-biodegradable (AOB) film with the function of enhanced freshness of food by employing oxo-biodegradable masterbatch (MB) and antimicrobial MB together with organic metal salt, organic acid, or unsaturated fatty acid. Antibacterial test of the AOB film with the different contents of the antimicrobial MB resulted in the significant freshness extension of plum. Tensile strength and elongation rate of the AOB films before UV treatment were similar to those of polyethylene films used as control. The reduced mechanical properties of AOB film after UV treatment (340 nm) suggested that the AOB film could be degraded by oxo biodegradation. The developed AOB films can effectively prevent decomposition of food by providing antibacterial function and preserving freshness.

• Food Science and Preservation
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• v.7 no.1
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• pp.57-62
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• 2000

Ginseng was stored at a temperature lower than the freezing point after a treatment of freezing point depressing agents to extend its freshness. Respiration rate at freezing point of ginseng, -1.7${\pm}$0.1$^{\circ}C$, was inhibited 92% and 97% compared with those stored at 5$^{\circ}C$or 20$^{\circ}C$ , respectively. Sorbitol solution chosen as a freezing point depressing agent lowered the freezing point of ginseng to about -3.0$^{\circ}C$. Ginsengs treated with the sorbitol solution and packaged with 0.06mm LDPE was stored at -2$^{\circ}C$ , and the quality change was then compared with ginsengs stored at 0$^{\circ}C$ and 5$^{\circ}C$. Weight loss of ginsengs stored at -2$^{\circ}C$ for 100days was 1.5%, which is about 2.6times less than those stored at 5$^{\circ}C$. However, there were no significant difference between the ginsengs stored at -2$^{\circ}C$ and at 0$^{\circ}C$(1.9%). Spoilage rate of the ginsengs was 100% after 50 days of storage at 5$^{\circ}C$ and 25% after 100days at 0$^{\circ}C$respectively. but that of ginsengs stored at -2$^{\circ}C$ was 13%, which was half than that of ginsengs stored at 0$^{\circ}C$. Firmness and amount of monoscaccharides in ginsengs were decreased during storage at 5 or 0$^{\circ}C$ but ginsengs stored at -2$^{\circ}C$ showed better firmness and an increase in monosaccharides such as fructose and glucose. From above, when ginseng treated with freezing pont depressing agents were stored at -2$^{\circ}C$, the shelf life was extended to 2 or 3 times longer than those that were stored at 5 or 0$^{\circ}C$, respectively.

• Archives of Pharmacal Research
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• v.26 no.1
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• pp.76-82
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• 2003

Drug releasing porous poly($\varepsilon$-caprolactone) (PCL)-chitosan matrices were fabricated for bone regenerative therapy. Porous matrices made of biodegradable polymers have been playing a crucial role as bone substitutes and as tissue-engineered scaffolds in bone regenerative therapy. The matrices provided mechanical support for the developing tissue and enhanced tissue formation by releasing active agent in controlled manner. Chitosan was employed to enhance hydrophilicity and biocompatibility of the PCL matrices. PDGF-BB was incorporated into PCL-chitosan matrices to induce enhanced bone regeneration efficacy. PCL-chitosan matrices retained a porous structure with a 100-200 $\mu$m pore diameter that was suitable for cellular migration and osteoid ingrowth. $NaHCO_3$ as a porogen was incorporated 5% ratio to polymer weight to form highly porous scaffolds. PDGF-BB was released from PCL-chitosan matrices maintaining therapeutic concentration for 4 week. High osteoblasts attachment level and proliferation was observed from PCL-chitosan matrices. Scanning electron microscopic examination indicated that cultured osteoblasts showed round form and spread pseudopods after 1 day and showed broad cytoplasmic extension after 14 days. PCL-chitosan matrices promoted bone regeneration and PDGF-BB loaded matrices obtained enhanced bone formation in rat calvarial defect. These results suggested that the PDGF-BB releasing PCL-chitosan porous matrices may be potentially used as tissue engineering scaffolds or bone substitutes with high bone regenerative efficacy.

• Journal of Microbiology and Biotechnology
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• v.22 no.7
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• pp.883-888
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• 2012

The efficacy of cowdung, Bangladesh Institute of Nuclear Agriculture (BINA)-biofertilizer, and Bangladesh Agricultural University (BAU)-biofungicide, alone or in combination, was evaluated for controlling foot rot disease of lentil. The results exhibited that BINA-biofertilizer and BAU-biofungicide (peat soil-based Rhizobium leguminosarum and black gram bran-based Trichoderma harzianum) are compatible and have combined effects in controlling the pathogenic fungi Fusarium oxysporum and Sclerotium rolfsii, which cause the root rot of lentil. Cowdung mixing with soil (at 5 t/ha) during final land preparation and seed coating with BINA-biofertilizer and BAU-biofungicide (at 2.5% of seed weight) before sowing recorded 81.50% field emergence of lentil, which showed up to 19.85% higher field emergence over the control. Post-emergence deaths of plants due to foot rot disease were significantly reduced after combined seed treatment with BINA-biofertilizer and BAU-biofungicide. Among the treatments used, only BAU-biofungicide as the seed treating agent resulted in higher plant stand (84.82%). Use of BINA-biofertilizer and BAU-biofungicide as seed treating biocontrol agents and application of cowdung in the soil as an organic source of nutrient resulted in higher shoot and root lengths, and dry shoot and root weights of lentil. BINA-biofertilizer significantly increased the number of nodules per plant and nodules weight of lentil. Seeds treating with BAU-biofungicide and BINA-biofertilizer and soil amendment with cowdung increased the biomass production of lentil up to 75.56% over the control.

• Research in Plant Disease
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• v.18 no.1
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• pp.40-44
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• 2012

Xanthomonas arboricola pv. pruni is the causal agent of bacterial black spot disease on some stone fruits, e.g. peach, plum and apricot. To evaluate pathogenicity of Xanthomonas arboricola pv. pruni strain from plum, inoculum of the isolated strain was spray inoculated to fruits and leaves of apricot, Japanese apricot and plum. Apricot and Japanese apricot showed severe black spot symptoms on fruits and shot hole symptoms on leaves. In case of apricot, about fifty percent of fruits did not grow and dropped by hypersensitive reaction to spray infection. Plum, cv. Formosa was very susceptible, showing severe black injury lesions on fruits and cankers on leaves and new twigs. On the other hand, plum cv. Daesukjosaeng, was highly resistant. Fruits of several plum cultivars such as Formosa and Chuhee were severely infected at natural infected orchards by X. arboricola pv. pruni. Where as those of Daesukjosaeng, Taeyang, Soldam and Hongrogen were moderately infected.

• Korean Journal of Agricultural Science
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• v.47 no.4
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• pp.1071-1081
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• 2020

Essential oils (EOs) have been shown to be plant-extracted antimicrobial agents. However, there are limited studies investigating the efficacy of EOs against pathogens. Among them, tea tree oil (TTO) is extracted from Melaleuca alternifolia, which is also used as an antifungal agent. In this study, the effect of TTO was investigated on the suppression of melon powdery mildew caused by Podosphaera xanthii and tomato leaf mold disease caused by Passalora fulva. Both powdery mildew and leaf mold diseases were significantly suppressed by a spray of TTO. Eighty percent of powdery mildew and 81% of leaf mold disease of the control value were suppressed by 0.5% TTO liquid, when sprayed 3 times every 7 days on the melon and tomato leaves. Inhibition of mycelial growth was also greatly affected by different concentrations of TTO against four different fungal pathogens. Ninety-eight percent of Pseudocercospora fuligena, 97% of P. fulva, 95% of Botrytis cinerea, and 94% of Phytophthora infestans mycelial growth were inhibited by 0.2% to 1.0% of TTO contained in plate media, respectively. However, phytotoxicity in plants by the TTO treatments was revealed when melon and tomato leaves were sprayed with a 1% and 2% concentration of TTO, respectively. Therefore, our findings show that TTO has high antifungal effects against various plant pathogens that occur during crop cultivation. We also suggest that when applying TTO to plant leaves, it is necessary to establish an accurate treatment concentration for different crops.

• The Korean Journal of Physiology and Pharmacology
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• v.18 no.5
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• pp.441-446
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• 2014

Ursolic acid (UA), a type of pentacyclic triterpenoid carboxylic acid purified from natural plants, can promote skeletal muscle development. We measured the effect of resistance training (RT) with/without UA on skeletal muscle development and related factors in men. Sixteen healthy male participants (age, $29.37{\pm}5.14$ years; body mass index=$27.13{\pm}2.16kg/m^2$) were randomly assigned to RT (n=7) or RT with UA (RT+UA, n=9) groups. Both groups completed 8 weeks of intervention consisting of 5 sets of 26 exercises, with 10~15 repetitions at 60~80% of 1 repetition maximum and a 60~90-s rest interval between sets, performed 6 times/week. UA or placebo was orally ingested as 1 capsule 3 times/day for 8 weeks. The following factors were measured pre-and post-intervention: body composition, insulin, insulin-like growth factor-1 (IGF-1), irisin, and skeletal muscle strength. Body fat percentage was significantly decreased (p<0.001) in the RT+UA group, despite body weight, body mass index, lean body mass, glucose, and insulin levels remaining unchanged. IGF-1 and irisin were significantly increased compared with baseline levels in the RT+UA group (p<0.05). Maximal right and left extension (p<0.01), right flexion (p<0.05), and left flexion (p<0.001) were significantly increased compared with baseline levels in the RT+UA group. These findings suggest that UA-induced elevation of serum irisin may be useful as an agent for the enhancement of skeletal muscle strength during RT.

• Journal of Life Science
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• v.19 no.5
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• pp.684-687
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• 2009

Xanthomonas arboricola pv. pruni, the causal agent of bacterial shot holes in stone fruits, was known to have a low population diversity. To investigate the genetic characteristics of X. arboricola pv. pruni isolated in Korea, three strains which have identical 16S rDNA sequences - including type strain (LMG852), Japanese isolate (MAFF301420) and Korean isolate (XWD1) - were analysed based on the nucleotide sequences of three DNA regions and RAPD pattern. No sequence diversity among the three strains was found within the ITS, glnA and atpD gene sequences. However, five of 756 nucleotides of the atpD gene determined (accession number FJ429319) were different from those of the French strain available from the Genbank database. RAPD analyses performed with 40 different arbitrary primers revealed that two strains isolated from Korea and Japan showed similarity in their band patterns distinguished by type strain. These results suggest that Korean and Japanese strains are very close and belong to a population with a low genetic diversity, and might have a different origin from strains found in West Europe.

• Applied Biological Chemistry
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• v.48 no.2
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• pp.150-154
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• 2005

To overcome the problems associated with chemical pesticides, biological pest controls agent extracted from Ginkgo biloba was studied. Insecticidal activities components in Ginkgo biloba extracts were analyzed using high performance liquid chromatography (HPLC). This results of HPLC analysis, GG-W80 were included bilobalide $611\;{\mu}g/kg$, ginkgolide A $37\;{\mu}g/kg$ and ginkgolide B $243\;{\mu}g/kg$, while YG-W80 were included bilobalide $214\;{\mu}g/kg$ and ginkgolide B $46\;{\mu}g/kg$. The biological activity of Ginkgo biloba extracts were conducted to repellent and pesticidial effect of Tetranichus urticae, Aphis gossypii and Myzus persicae treated with Ginkgo biloba leaves extracts. Mortalities of adult T. urticae to green Ginkgo biloba extracts (GG-W80) and yellow Ginkgo biloba extracts (YG-W80) were shown 98.3% and 20.0%, respectively. From these results, terpenes components in Ginkgo biloba extracts could be use for biological controls for T. urticae.