• Title/Summary/Keyword: Ext

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Isolation of Alkalophilic, Thermophilic Bacillus sp. TA-11 and Production of $\beta$-Galactosidase (호알칼리성, 고온성 Bacillus sp. TA-11의 분리와 $\beta$-galactosidase의 생산)

  • Lee, Jong-Su;Gwak, In-Yeong;Geum, Jong-Hwa
    • The Journal of Natural Sciences
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    • v.5 no.1
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    • pp.47-52
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    • 1992
  • A alkalophilic, thermophilic bacterium TA-11 which produce $\beta$-galactosidase highly was isolated from compost and identified to the genus Bacillus. $\beta$-galactosidase production was maximized when it was incubated in synthetic medium containing 1.5% lactose. 0.4% peptone, 0.4% teast ext., 0.2% $MgSO_4$ 0.05% $NH_4$Cl and 0.2% NacL(initial pH; 10.0) at $50^{\circ}C$ for 2 days in reciprocal shaker.

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Production of High Viscous Hyaluronic Acid Complex from Klebsiella sp. L-10 (Klebsiella sp. L-10에 의한 고점성 히아루론산 복합체의 생산)

  • Lee, Hyang-Suk;Choe, Yeong-Jun;Lee, Jong-Su
    • The Journal of Natural Sciences
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    • v.7
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    • pp.59-65
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    • 1995
  • A bacterium which produce hyaluronic acid complex was isolated from soil, and identified as Klebsiella sp. L-10. The maximal hyaluronic acid complex production was obtained when the strain was cultured at $37^{\circ}C$ for 18hrs with shaking in the optimal medium containing 0.1% yeast ext., 3% tryptone, 5% glucose, 10mM $K_2HPO_4$and $KH_2PO_4$, respectively and initial pH6.5 and the final hyaluronic acid complex production under the above condition was 1,400mg per liter of cultures.

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Performance Analysis of Block Write Operation of File Systems on Linux Environment (리눅스 환경에서 파일 시스템들의 블록 쓰기 연산 성능 분석)

  • Choi, Jin-Oh
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.19 no.1
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    • pp.136-140
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    • 2015
  • Linux environment that is commonly used at embedded systems supports various file systems as Ext2, FAT, NTFS, etc. The file system that is equiped on the embedded system is mostly implemented on mini hard disk or flash memory. The types of the file system of the system make an effect on the performance of a application programs. The factors of file system performance on a same media are block read, block write and block free time. On these factors, block read and block free time are not so different according to the type of file systems. This paper evaluates the performance benchmark of file systems supported by linux about block allocation and write performance. The results obtained from various experiments shows the characteristics of each file system.

Comprehensive study of components affecting extrinsic transconductance in In0.7Ga0.3As quantum-well high-electron-mobility transistors for image sensor applications (이미지 센서 적용을 위한 In0.7Ga0.3As QW HEMT 소자의 extrinsic trans-conductance에 영향을 미치는 성분들의 포괄적 연구)

  • Yun, Seung-Won;Kim, Dae-Hyun
    • Journal of Sensor Science and Technology
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    • v.30 no.6
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    • pp.441-445
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    • 2021
  • The components affecting the extrinsic transconductance (gm_ext) in In0.7Ga0.3As quantum-well (QW) high-electron-mobility transistors (HEMTs) on an InP substrate were investigated. First, comprehensive modeling, which only requires physical parameters, was used to explain both the intrinsic transconductance (gm_int) and the gm_ext of the devices. Two types of In0.7Ga0.3As QW HEMT were fabricated with gate lengths ranging from 10 ㎛ to sub-100 nm. These measured results were correlated with the modeling to describe the device behavior using analytical expressions. To study the effects of the components affecting gm_int, the proposed approach was extended to projection by changing the values of physical parameters, such as series resistances (RS and RD), apparent mobility (𝜇n_app), and saturation velocity (𝜈sat).

Phytochemical constituents of Lactuca serriola leaves and their content analysis by HPLC-UV

  • Kim, Juree;Lee, Hak-Dong;Choi, Jungwon;Lee, Sanghyun
    • Journal of Applied Biological Chemistry
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    • v.65 no.3
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    • pp.153-158
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    • 2022
  • This study aimed to identify the phytochemical constituents of Lactuca serriola leaves and perform quantitative analysis of the methanol (MeOH) extract of L. serriola, L. indica, L. raddeana, L. sativa, and L. triangulata. Six compounds were isolated from the MeOH extracts of L. serriola using open column chromatography and identified as protocatechuic acid (1), caffeic acid (2), cynaroside (3), apigenin 7-glucuronide (4), luteolin (5), and apigenin (6) using 1H-, 13C-nuclear magnetic resonance, and mass spectrometry. Quantitative analysis of the six compounds was performed on the MeOH extract of Lactuca species using high-performance liquid chromatography (HPLC) and an ultraviolet (UV). A reverse-phased column was used, and the UV absorbance was set to 280 nm. The contents of compounds 2 and 3 were the highest (1.58 and 2.64 mg/g ext., respectively) in L. serriola extracts. However, compounds 4 and 6 were higher (1.46 and 0.40 mg/g ext., respectively) in L. triangulata. These results provide quantitative data for the application of Lactuca species in the pharmaceutical and functional food industries.

A Case Report on Korean Medicine Treatment for a Patient Diagnosed with Acute Gastric Mucosal Lesion on Ultrasound Imaging (초음파 영상에서 급성 위점막 병변으로 진단된 환자에 대한 한의치료 치험 1례)

  • Chae-eun Kim;Tae-hyun Baek
    • The Journal of Internal Korean Medicine
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    • v.44 no.6
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    • pp.1354-1361
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    • 2023
  • Objectives: This study reports a clinical case that showed improvement in the symptoms of a patient with acute gastric mucosal lesion (AGML) through ultrasound. Methods: A 90-year-old woman with AGML was treated with Korean medicine treatments, including Hyangsapyeongwisan Soft Ext, acupuncture, and moxibustion. The responses before and after treatment were evaluated using ultrasound imaging and the numerical rating scale (NRS). Results: After treatment, ultrasound imaging showed improvement in gastric mucosa thickness, from 1.45 cm to 0.53 cm at the anterior wall and from 1.25 cm to 0.36 cm at the posterior wall. The NRS scores for epigastric pain, nausea, and dizziness all decreased, and the symptoms of diarrhea disappeared. Conclusion: This study suggests that Korean medicine treatment can be a therapeutic option in treating the symptoms of patients with AGML.

Effect of Harvest Time on Seed Quality of Silage Corn Inbreds and Hybrids

  • Lee, Suk-Soon;Yun, Sang-Hee;Soo, Jung-Moon;Min, Hwang-Kee;Ryu, Si-Hwan;Park, Jong-Yeol
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.5
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    • pp.361-367
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    • 2002
  • In order to determine the optimum harvest time for the seed production of inbreds and hybrids in silage corn, the ears of sib-pollinated 'KS5', 'KS7rhm', and 'Ga209' and cross-pollinated 'KS5' $\times$ 'KS6' (Suwon19), 'KS7 rhm' $\times$ 'KSl17' (Suwonok), and 'Ga209' $\times$ 'DB544'(Kwanganok) were harvested at the one-week intervals from 4 to 10 weeks after silking. The optimum harvest time for the seed production for 'KS5', 'KS5' $\times$ 'KS6', 'KS7 rhm', and 'KS7rhm' $\times$ 'KS117' was 7 weeks after silking considering both emergence rate and plumule growth in cold test. Although earlier harvested seeds showed similar germination rate as the seeds harvested at the optimum time at $25^{\circ}C$, their emergence rate were lower in cold test. Seed weight and $\alpha$-amylase activity of earlier harvested seeds were lower compared to those of seeds harvested at the optimum time, while leakage of total sugars and electrolytes were higher. However, the later harvested seeds showed lower germination rates at $25^{\circ}C$ and emergence rates in cold test probably due to the lower $\alpha$-amylase activity although they showed increased seed weight and reduced leakage of total sugars and electrolytes. In contrast, the emergence rate of 'Ga209' and 'Ga209' $\times$ 'DB544' in cold test increased up to 10 weeks after silking probably due to the increased seed weight and $\alpha$-amylase activity and reduced sugar and electrolyte leakages during the germination. The cross-pollinated F$_1$ hybrid seeds showed higher germination and emergence rates at $25^{\circ}C$ and in cold test, and higher plumule growth and $\alpha$-amylase activity compared to those of sib-pollinated inbreds.

Production of Pups Following Artificial Insemination by Canine Intrauterine Inseminator (개 자궁내 인공수정기에 의한 인공수정 후 산자생산)

  • 공일근;조성균;임용택;이상인;위성하
    • Journal of Veterinary Clinics
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    • v.16 no.2
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    • pp.375-380
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    • 1999
  • This study was conducted to develop an intrauterine inseminator (IUI) to deposit of frozen semen into uterus and to evaluate the results obtained after artificial insemination by IUI. Two Japanese spitzs (2 to 4 years of age) were used as semen donors. Semen was collected by manual masturbation into sterile glass collection tubes and separated into 3 fractions with only the sperm-rich fractions retained for further examination. Sperm motility >70%, sperm concentration of 200 to $400{\times}10^6 cells/ml$$\times$g for 5 min and poured out the suspended solution, and then diluted with 2 ml Tris-buffer which was consisted of 2.4 g Tris, 1.4 g citric acid, 0.8 g glucose, 0.1 $\mu\textrm{g}$/ml streptomycin, 100 IU/ml penicillin, 20 ml egg yolk to 100 ml mili-Q water (Ext I) or supplemented with 8 ml glycerol and 1 ml Equex STM paste to 100 rnl (Ext II). The diluted semen was cooled to 5$^{\circ}C$ in cold room, where the temperature in the sample reached 5$^{\circ}C$. Two h after beginning the cooling procedure, 2 ml of Ext II, also at 5$^{\circ}C$, was added and mixed by gently reversing the tubes several times during 1 h. The final sperm concentration for freezing was approximately $50{\times}10^6 cells/ml$. After equilibration, the semen was loaded into 0.5 ml straw and frozen on the liquid nitrogen vapour in styrofoam box. The straws were thawed at 7$0^{\circ}C$ for precisely 6 sec. After thawing of each straw, the frozen semen can survived over 50% motility. All the females were inseminated twice with 1 ml of $25{\times}10^6 cells/ml$

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Physicochemical Characteristics of Concentrates and Dried Powder Extracted from Herbal Plant Mixture (약용식물자원의 배합비와 제형이 추출물의 품질특성에 미치는 영향)

  • Cho, Yong-Sik;Park, Shin-Young;Choi, Yoon-Hui;Kim, Eun-Mi;Kwon, Hye-Ran;Lee, Kyung-Ha
    • Proceedings of the Korean Society of Community Living Science Conference
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    • 2009.09a
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    • pp.95-95
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    • 2009
  • 우리나라를 비롯한 동양문화권에서는 천연 약용식물자원의 이용을 통하여 전통적으로 지역 보건향상을 도모해 왔으며 최근 성인병과 난치병 해결을 위한 대체의학에 대한 관심이 증가하면서 약용식물자원을 이용한 건강기능식품 분야가 주목되고 있다. 약용식물자원은 민간에서 약선음식 등에 널리 활용되고 있지만 원료 농산물을 그대로 이용하는 수준으로 광범위한 적용성을 가지는 약선식품용 소재화 기술개발은 약용식물자원의 소비촉진에 크게 기여할 것으로 기대된다. 본 연구에서는 약선식품용 식품소재 개발의 일환으로 약용식물자원의 배합비와 추출물의 제형이 품질특성에 미치는 영향을 조사하였다. 스트레스 경감기능을 보유하는 약용식물자원은 고문헌에 기초하여 선정하였으며 약선원리에 따라 소정의 조건으로 3종(CLP 1, CLP 2, CLP 3) 배합비를 설정하였다. 추출물은 온도를 달리한 열수에 추출하고 유동Ext, 분무건조분말 및 과립으로 제형화 하였고 품질특성은 폴리페놀함량, 용해도, 투과도 및 색도특성을 분석하였다. 약용식물자원 혼합 추출물의 수율은 CLP 2가 65%로 가장 많았으며 1차 추출에서 76%의 수율을 보였고 추출시간대비 수율은 상업적 추출의 경우가 가장 높았다. 유동 Ext의 폴리페놀함량은 CLP 2가 g 당 11~13 mg을 함유하여 가장 많았고 CLP1과 CLP 2는 동등한 수준을 나타냈다. 유동 Ext는 99%의 용해도를 보였으며 투과도는 CLP 3가 52~68%로 가장 낮았으며 추출조건에 따라 색도가 차이가 있었다. 분말형태의 경우 폴리페놀 함량은 분무건조로 제조한 CLIP 2가 15.40mg/g으로 가장 많았다. 부형제로 과립화한 CLP 3가 g당 1.7 mg으로 가장 적었다. 분무건조분말은 98~99%의 용해도를 보였으며 부형제를 사용하여 분무건조하거나 과립화한 시료는 투과도와 명도가 높았으며 적색도와 황색도 및 갈색도가 낮았다. 이상의 결과는 약용식물자원 혼합 추출물은 원료의 조합비와 추출물의 제형은 식품소재화에 따른 품질특성의 영향인자로 작용함을 나타낸다.

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Optimizing a Multimedia File System for Streaming Severs (스트리밍 서버를 위한 멀티미디어 파일 시스템 최적화)

  • 박진연;김두한;원유집;류연승
    • Journal of KIISE:Computer Systems and Theory
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    • v.31 no.5_6
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    • pp.268-278
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    • 2004
  • In this paper, we describe our experience in the design and implementation of the SMART file system to handle multimedia workload. Our work has three design objectives: (ⅰ) efficient support for sequential workload, (ⅱ) avoiding disk fragmentation, (ⅲ) logical unit based file access. To achieve these three objectives, we develop a file system where a file consists of linked list of Data Unit Group. Instead of tree like structure of the legacy Unix file system, we use single level file structure. Our file system can also access the file based upon the logical unit which can be video frame or audio samples. Data Unit Group is a group of logical data units which is allocated continuous disk blocks. At the beginning of each Data Unit Group, there exists an index array. Each index points to the beginning of logical data units, e.g. frames in the Data Unit Group. This index array enables the random access and sequencial access of semantic data units. SMART file system is elaborately tailored to effectively support multimedia workload. We perform physical experiments and compare the performance of SMART file system with EXT2 file system and SGI XFS file system. In this experiment, SMART file system exhibits superior performance under streaming workload.