• 한국실내디자인학회논문집
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• 제24권5호
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• pp.51-61
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• 2015

The purposes of this study were to examine the characteristics of elements composing the cultural identity of Chinese architectural and spatial design, and to analyze the expression characteristics and influencing factors of contemporary restaurant design. With these purposes, first, this study reviewed related literature in order to find the characteristics of tangible elements observed in intangible elements and space expression characteristics forming the cultural identity of China. Second, this study conducted a case study in order to understand the expression characteristics of the design of Chinese restaurants in Shanghai and to find factors influencing the design. In order to sample the subjects of the case study, the researcher selected 18 restaurants that were recommended repeatedly in books on Chinese restaurants and tourism and major Internet sites or highly evaluated by visitors, and then choose 15 of them that were found to be fit for the purpose of the survey. According to the result of the survey, the restaurants were largely grouped by type into 10 cases of fine dining, 3 of middle-class casual dining, 1 of fast food, and 1 of luxury tea house serving traditional tea and snacks. Of these restaurants, 46.7% (7 cases) were of CM-CM-CM type, namely, using contemporary simple elements in all of external spaces, indoor spaces, and decorations, 20% (3 cases) were of CM-CM-CT type, using contemporary and simple external and indoor spaces, and traditional furniture or articles, 20% (3 cases) were of CT-CM-CT type, using traditional external spaces such as traditional buildings, contemporary simple finishing of indoor spaces, and traditional articles such as palace lanterns and traditional doors and windows, and 13.3% (2 cases) were of CT-CT-CT type, using traditional elements in their original forms in general. Through this research and survey, cultural identity showing Chinese traditions observed in the expression characteristics of restaurant spatial designs was confirmed, and it was also found that the identity was underlain by ideologies, values, lifestyles, etc. that had long accumulated through the long history of the country and exerted a great influence.

• 한국동물학회지
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• 제38권4호
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• pp.505-513
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• 1995

P-lement의 germ line transformation을 이용하여 초파리 난소에서의 세포특이적 유전자발현 양상을 조사하였다. 표지유전자인 lacZ의 발현양상은 생식세포, 체세포, 두 종류의 세포에서 모두 발현되는 경우로 대별되며, 생식세포에서 lacZ의 발현은 조세포 또는 조세포와 난자에서 동시적으로 발현되는 경우로 구분되었다. 난자주변 난포세포에서의 lacZ 발현은 난자형성 9-10기 난포의 경우 모든 세포에서 균일하게 발현되거나 일부 세포에서만 발현되는 양상으로 구분되었다. 일부의 난포세포에서 발현되는 경우 난자와 조세포 경계의 난포세포에서 발현되는 경우, 이들을 제외한 난포세포에서 발현되는 경우로 구분되었다. 난자주위의 난포세포에서 난자의 전후축을 따른 구배현상도 관찰되었다. 극세포 또는 경계세포에서 발현되는 3개의 독립라인을 분리하였다. 독립된 EDL의 난소에서 나타난 lacZ의 발현양상은 초파리 난자형성에 관여하는 유전자의 시간과 공간적인 발현을 반영하며 이들 유전자 분리의 기초를 제공할 것으로 사료된다.

• 아동학회지
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• 제24권6호
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• pp.33-46
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• 2003

This study investigated age differences in children's expression of pride and shame by their age, and type and level of task difficulty when they succeeded of failed on tasks. The subjects were 55 4-year-old children from 2 day-care centers and 37 7-year-old children from I elementary school. Each child participated in pride and shame assessment sessions. Data were analyzed by repeated measures ANOVA, t-test, paired t-test, Pearson's correlations. When they succeeded on tasks, 4-year-olds showed more pride than 7-year-olds. More pride was shown when subjects succeeded on difficult than on easy tasks. An interaction effect for pride was found between age, and type and level of task difficulty. Seven-year-olds showed more shame than 4-year-olds when they failed on tasks. More shame was shown when subjects failed on easy than on difficult tasks. The expression of pride was positively related to the expression of shame.

• Asian-Australasian Journal of Animal Sciences
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• 제25권7호
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• pp.1021-1028
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• 2012

Peripheral blood mononuclear cells (PBMCs) discriminate microbial pathogens and induce T-cell responses of appropriate effector phenotype accordingly. Toll-like receptors (TLRs), in part, mediate this microbial recognition and differentiation while the development of T-cell effector functions critically depends on the release of Th1- or Th2- type cytokines. In the present study, buffalo PBMCs were stimulated under in vitro culture conditions by Bacillus subtilis cell wall petidoglycan, a TLR2 ligand, in a dose- and time- dependent manner. The expression of TLR2 as well as the subsequent differential induction of the Th1 and Th2 type cytokines was measured. Stimulation was analyzed across five doses of peptidoglycan ($10{\mu}g/ml$, $20{\mu}g/ml$, $30{\mu}g/ml$, $40{\mu}g/ml$ and $50{\mu}g/ml$) for 3 h, 12 h, 24 h and 36 h incubation periods. We observed the induction of TLR2 expression in a dose- and time-dependent manner and the peptidoglycan induced tolerance beyond $30{\mu}g/ml$ dose at all incubation periods. The correlation between peptidoglycan stimulation and TLR2 induction was found positive at all doses and for all incubation periods. Increased production of all the cytokines was observed at low doses for 3 h incubation, but the expression of IL-4 was relatively higher than IL-12 at the higher antigen doses, indicating tailoring towards Th2 response. At 12 h incubation, there was a pronounced decrease in IL-4 and IL-10 expression relative to IL-12 in a dose- dependent manner, indicating skewing to Th1 polarization. The expression of IL-12 was highest for all doses across all the incubation intervals at 24 h incubation, indicating Th1 polarization. The relative expression of TNF-${\alpha}$ and IFN-${\gamma}$ was also higher while that of IL-4 and IL-10 showed a decrease. For 36 h incubation, at low doses, relative increase in the expression of IL-4 and IL-10 was observed which decreased at higher doses, as did the expression of all other cytokines. The exhaustion of cytokine production at 36 h indicated that PBMCs became refractory to further stimulation. It can be concluded from this study that the cytokine response to sPGN initially was of Th2 type which skews, more pronouncedly, to Th1 type with time till the cells become refractory to further stimulation.

• 한국실내디자인학회:학술대회논문집
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• 한국실내디자인학회 1999년도 춘계학술발표대회 논문집
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• pp.122-125
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• 1999

The purpose of this study is to dlassify into types of traditionality reflected in modern Korean houses in 1990's designed by architec Modern houses were divided into five types through qualitative analysis. Analysis is categorized into three parts, space-pl structural elements ornamental elements. Characteristics of each type were defined as 'Indicative expression of the traditionalit', 'Decorative expression of the traditionality', 'Eclectic expression of the traditionality', 'Contemporary expression of the traditionalit', 'Abstract expression of the traditionality'. The results showed that 'Contemporary or Abstract expression of traditionality(type IV and V) were major types of modern Korean houses expressed traditionality. This reveals that in order to apply traditionality to the modern houses, simplifed and sbstracted for were preferred comparing with the direct imitation of traditional forms.

• 한국미생물·생명공학회지
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• 제21권3호
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• pp.214-220
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• 1993

The mating type a of yeast, Saccharomyces cerevisiae mutant with hmla2-102 and sir3-8ts was changed to type alpha by changing the growth temperature from 25C to 35C. A temperature-sensitive expression vector system was constructed using mating factor alpha1 (Mfalpha1) gene encoding alpha factor which is expressed in the type alpha cells. Vectors with different copy numbers were constructed by joining the promoter and pre or prepro-secretion single sequence of Mfalpha1 to promoterless PHO5' gene as a reporter gene.

• 동양고전연구
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• 제57호
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• pp.467-505
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• 2014

필자(筆者)는 여러 해째 간찰(簡札)를 대상으로 일련(一連)의 국어학적(國語學的) 연구(硏究)를 진행하고 있으며 이 연구(硏究)는 그 일환(一環)으로서 [안부인사(안부인사(安否人事))]의 유형화(類型化)를 위한 것이다. 이를 위하여 근대국어(近代國語) 시기(時期)(16세기(世紀)-19세기(世紀))의 한문간찰(漢文簡札)와 한글간찰(簡札)를 대상으로 [안부인사(安否人事)]를 형식적(形式的) 유형(유형(類型))와 의미적(意味的) 유형(類型)로 나누어 분석(分析)하였다. [안부인사(安否人事)]의 형식적(形式的) 유형(類型)는 정치여부(定置與否), 생략여부(省略與否), 기상여부(寄狀與否), 존비여부(尊卑與否)를 기준으로 나눌 수 있다. 정치여부(定置與否)를 제1기준으로, 생략여부(省略與否)를 제2기준으로 삼아 다섯 가지로 유형화하였다. [안부인사(安否人事)]의 전형성(典型性)이 가장 잘 드러난 정치형(定置型)이면서 완성형(完成型)을 제(第)1유형(類型)로 삼았다. 정치형(定置型)이면서 자기안부생략형(自己安否省略型)을 제(第)2유형(類型)으로, 정치형(定置型)이면서 상대안부생략형(相對安否省略型)을 제(第)3유형(類型)으로, 정치형(定置型)이면서 안부인사생략형(安否人事省略型)을 제(第)4유형(類型)로 삼았다. [안부인사(安否人事)]에서 破格이 가장 심한 도치형(倒置型)을 제(第)5유형(類型)로 삼았다. 第1유형(類型)은 [상대안부(相對安否)]가 [자기안부(自己安否)]에 선행(先行)하는 정치형(定置型)이면서 의미요소(意味要素)가 온전한 완성형(完成型)을 가리킨다. 이 유형은 [안부인사(安否人事)]의 모든 구성요소(構成要素)를 갖췄다는 점에서 가장 전형적(典型的)이며 규범적(規範的)이라 할 수 있다. 제(第)2유형(類型)은 [안부인사(安否人事)]가 [상대안부(相對安否)]로만 이루어진 것이다. 정형성(定型性)에서는 제(第)1유형(類型)보다 덜하지만 출현빈도(出現頻度)에서는 큰 차이가 없는 유형이다. [상대안부(相對安否)]를 충실히 묻되, [자기안부(自己安否)]는 생략하는 것이 예의에 크게 어긋나지 않으면서 간찰을 쉽게 쓸 수 있기 때문에 활용된다. 제(第)3유형(類型)은 [상대안부(相對安否)+자기안부(自己安否)]의 구성을 보이는 정치형(定置型)이지만 [상대안부(相對安否)]가 생략된 유형을 가리킨다. 제(第)4유형(類型)은 [상대안부(相對安否)+자기안부(自己安否)]의 구성을 보이는 정치형(定置型)이지만 [안부인사(安否人事)] 전체가 생략된 유형이다. 이 유형은 [안부인사(安否人事)]자체를 완전히 생략한 경우와 '제번(除煩)'과 같은 상투적인 표현이 [안부인사(安否人事)]를 대신하는 경우로 나뉜다. 제(第)5유형(類型)은 정치형(定置型)과 달리 [자기안부(自己安否)+상대안부(相對安否)]의 구성을 보이는 도치형(倒置型)이다. 이 유형은 정형성(定型性)에서 파격(破格)이 가장 심한 유형으로 실예(實例)도 아주 드물다. [자기안부(自己安否)]를 앞세우고 나중에 체면치레로 [상대안부(相對安否)]를 묻는 것은 예의에 어긋나기 때문이다. 이 외에 [안부인사(安否人事)] 유형을 [상대안부(相對安否)]와 [자기안부(自己安否)]가 직접 연결된 직접형(直接型)과 [事緣]으로 분리된 간접형(間接型)으로 대별(大別)하여 세분할 수도 있다. [안부인사(安否人事)]의 의미적(意味的) 유형(類型)은 기상여부(寄狀與否), 완급여부(緩急與否), 친밀여부(親密與否), 격조여부(隔阻與否)를 기준으로 나눌 수 있다. 기상(寄狀)의 경우 [안부인사(安否人事)]는 [상대안부(相對安否)(시후(時候)+문안(問安)+심경(心境))+자기안부(自己安否)(상황(狀況)+안부(安否)+심경(心境))]의 구성을 보인다. [상대안부(相對安否)]에서 [시후(時候)]는 [계절(季節)] 정보와 [기상(氣象)(날씨)] 정보로 세분(細分)할 수 있고, [심경(心境)] 또한 수신자(受信者)의 [가족안부([家族安否) 심경(心境)]과 [개인안부(個人安否) 심경(心境)]으로 나뉜다. [자기안부(自己安否)]에서 [상황(狀況)]은 수신자(受信者)의 종래 상황인 [근황(近況)]과 수신자(受信者)의 현재 상황인 [현황(現況)]으로 나뉘며 [문안(問安)] 또한 수신자(受信者)의 [가족문안(家族問安)]와 [개인문안(個人問安)]으로, [안부(安否)]는 [가족안부(家族安否)]와 [개인안부(個人安否)]로 세분(細分)할 수 있다. 이처럼 [문안(問安)]이나 [안부(安否)]가 [가족(家族)]과 [개인(個人)] 차원에서 짝을 이루어 쓰일때가 많은데 부모(父母)나 조부모(祖父母)를 모시고 사는 층층시하(層層侍下)의 대가족제도(大家族制度)에서 비롯한 현상이라 할 수 있다. 답상(答狀)의 경우 [안부인사(安否人事)]는 [상대안부(相對安否)(수신(受信)+안부(安否)+심경(心境))+자기안부(自己安否)(상황(狀況)+안부(安否)+심경(心境))]의 구성을 보며 [상대안부(相對安否)]에서만 의미구성이 기상(寄狀)과 차이가 있다. [상대안부(相對安否)]에서 [수신(受信)]은 직접 간찰을 받는 [서신(書信)]과 사람들 통해 소식을 전해 듣는 [전언(傳言)]으로 나뉘며 [안부(安否)]는 [가족안부(家族安否)]와 [개인안부(個人安否)]로, [심경(心境)] 역시 [가족안부(家族安否) 심경(心境)]과 [개인안부(個人安否) 심경(心境)]으로 나뉜다.

• Molecules and Cells
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• 제22권2호
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• pp.182-188
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• 2006

Dlx5 and Osx are master regulatory proteins essential for initiating the cascade leading to osteoblast differentiation in mammals, but the mechanism of osteoblast-specific expression is not fully understood. DNA methylation at CpG sequences is involved in tissue and cell type-specific gene expression. We investigated the methylation status of Dlx5 and Osx in osteogenic and nonosteogenic cell lines by methylationspecific PCR (MSP). The CpG dinucleotides of the Dlx5 and Osx promoter regions were unmethylated in osteogenic cell lines transcribing these genes but methylated in nonosteogenic cell lines. Treatment of C2C12 cells with 5-AzadC induced dose- and timedependent expression of Dlx5 and Osx mRNA by demethylating the corresponding promoters. Furthermore the mRNAs for the osteoblast markers ALP and OC, which were undetectable in untreated cells, gradually increased after 5-AzadC treatment. In addition, BMP-2 stimulation induced Dlx5 expression by hypomethylating its promoter. These findings suggest that DNA methylation plays an important role in cell type-specific expression of Dlx5 and Osx.

• 패션비즈니스
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• 제22권5호
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• pp.41-51
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• 2018

Cable knitting, one of the most representative structures of knitwear has been applied to a number of knit items in a variety of expressions every season. The purpose of this study is to analyze the various types of expressions applied with cable knitting techniques to make the necessary data for planning cable knit designs. The types of cable knitting were devide into two categories which are based on the cable knitting pattern and structure. Based on the pattern, firstly, formal type is a reproduction of traditional Aran knit style and is usually applied to a practical and comfortable timeless knit product. Secondly, informal type is irregular applied to patterns and directions to highlight the dynamics of cable patterns. Thirdly, contrasting type emphasizes the cable pattern by contrasting the color of the part of the cable pattern. Fourthly, figurative type is the expression of the conceptual motifs and using the cable knit pattern itself as motif. Based on the structure, firstly, open type is to emphasize patterns and spaces by utilizing open spaces created by the intersection of cabling stitches. Secondly, decorative type is to enhance the ornamental effect of cable knitting by various methods and other subsidiary materials. Thirdly, avant-garde type is applied in various ways with exaggerated volumes or textures using the three-dimensional effects of cable knitting. Through this study, we look forward to the possibility of expressing the unique aesthetic characteristics of each of the various knitting techniques.

• Molecules and Cells
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• 제45권12호
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• pp.950-962
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• 2022

Aging is a major risk factor for common neurodegenerative diseases. Although multiple molecular, cellular, structural, and functional changes occur in the brain during aging, the involvement of caveolin-2 (Cav-2) in brain ageing remains unknown. We investigated Cav-2 expression in brains of aged mice and its effects on endothelial cells. The human umbilical vein endothelial cells (HUVECs) showed decreased THP-1 adhesion and infiltration when treated with Cav-2 siRNA compared to control siRNA. In contrast, Cav-2 overexpression increased THP-1 adhesion and infiltration in HUVECs. Increased expression of Cav-2 and iba-1 was observed in brains of old mice. Moreover, there were fewer iba-1-positive cells in the brains of aged Cav-2 knockout (KO) mice than of wild-type aged mice. The levels of several chemokines were higher in brains of aged wild-type mice than in young wild-type mice; moreover, chemokine levels were significantly lower in brains of young mice as well as aged Cav-2 KO mice than in their wild-type counterparts. Expression of PECAM1 and VE-cadherin proteins increased in brains of old wild-type mice but was barely detected in brains of young wild-type and Cav-2 KO mice. Collectively, our results suggest that Cav-2 expression increases in the endothelial cells of aged brain, and promotes leukocyte infiltration and age-associated neuroinflammation.