• BMB Reports
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• v.32 no.4
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• pp.345-349
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• 1999

We fused the promoter region of an H3.2 chicken histone gene, whose expression is dependent on the cell cycle, to the 5' coding region of an H3.3 chicken histone gene, which is expressed constitutively at a low level throughout the cell cycle. This fusion gene showed a cell cycle-regulated pattern of expression, but in a different manner. The mRNA level of the fusion gene increase during the S phase of the cell cycle by about 3.7-fold at 6 h and 2.7-fold at 12 h after the serum stimulation. The mRNA level of the intact H3.2 gene, however, increased by an average of 3.6-fold at 6 h and 8.7-fold at 12 h. This different expression pattern might be due to the differences in their 3' end region that is responsible for mRNA stability. The 3' end of the H3.2 mRNA contains a stem-loop structure, instead of a poly(A) tail present in the H3.3 mRNA. We also constructed a similar fusion gene using a H3.3 histone gene whose introns had been eliminated to rule out the possibility of involvement of the introns in cell cycle-regulated expression. The expression of this fusion gene was almost identical to the fusion gene made previously. These results indicate that the promoter region of the H3.2 gene is only partially responsible for its expression during the S phase of the cell cycle.

• ETRI Journal
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• v.10 no.4
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• pp.89-98
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• 1988

We consider an N duplicate-server system, where each server consists of two reconfigurable duplicated units which are subject to breakdowns. This system is studied analytically using generating functions, and also numerically using the matrix-geometric procedure. Using the generating function approach we obtain a recursive expression of the queuelength distribution for N=1. This expression in difficult to generalize to N>1. The numerical method is applicable for any value of N. For any N, we also obtain the condition for stability and the availability of the system.

• Korean Journal of Microbiology
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• v.29 no.2
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• pp.80-84
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• 1991

We cloned and expressed hepatitis B viral core antigen (HBcAg) gene in E. coli using $P_{L}$ promoter system. For optimal expression of the gene, we undertook the studies on the effects of the distance between Shine-Dalgarno (SD) sequence and start codon, copy number of repressor gene, induction temperature, and the stability of the core antigen. The results demonstrated that the induction at 37.deg.C was more efficient than at 42.deg.C, and the 11 base pairs (bp) distance between SD sequence and start codon of HBcAg gene was more efficient than the 15 bp distance in E. coli. The copy number of cI857 repressor gene did not influence on the expression of HBcAg, and the expression level of HBcAg in mutant type (low protease activity) and wild type strains was almost the same. The produced core antigen appeared to be HBcAg not HBeAg judged by two different radioimmunoassat (RIA) kits. This result suggested that the antigen was stable in E. coli.i.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.4
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• pp.371-378
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• 2010

For sensitive and accurate gene expression analysis, normalization of gene expression data against housekeeping genes is required. There are conventional housekeeping gene (e.g. ACT) that primarily function as an internal control of transcription. In this study, we performed an in silico analysis of 278 rice gene expression samples (GSM) in order to identify the gene that is most consistently expressed. Based on this analysis, we identified novel candidate housekeeping genes that displayed improved stability among the cross experimental conditions. Furthermore four of the most conventional housekeeping genes were included in our 30 other housekeeping genes among the most stable genes. Therefore, these 30 genes can he used to normalize transcription results in gene expression studies on rice at a broad range of experimental conditions.

• Macromolecular Research
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• v.11 no.1
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• pp.19-24
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• 2003

Polymeric gene delivery system attracts profound attention as it shows less toxicity, versatility, and reasonable gene expression efficiency. Terplex system, a synthetic biopolymeric gene delivery system consisting of stearyl poly-L-lysine (stearyl-PLL) and low density lipoprotein (LDL) was evaluated for its body distribution of gene expression of exogenously administered pDNA after tail-vein injection in mice. Kidney and spleen are two major organs with highest gene expression, whereas liver and heart showed marginal gene expression among the organs examined. Hemolytic effect of the terplex system was evaluated using human red blood cells, where terplex system did not cause significant hemolysis at the concentrations above the experimental ranges, although unmodified PLL or stearyl-PLL without LDL did. Serum stability of terplex system against enzymatic degradation was also significantly enhanced, presumably due to the steric stabilization from the polymers. Based on these findings and along with its high in vitro transfection efficiency, terplex system could serve as a safe and efficient polymeric gene delivery system with many applications for the in vivo gene therapy.

• Proceedings of the Korean Society of Life Science Conference
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• 2008.10a
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• pp.69.2-69.2
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• 2008

• Proceedings of the KIEE Conference
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• 1994.07a
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• pp.114-118
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• 1994

A comprehensive analytical study of frequency-modulated supply of the dynamic instability in a variable-reluctance stepping motor, is described. It is shown that stability can be achieved by frequency modulation provided that the phase displacement between the modulating signal and the rotor velocity oscillation lies between certain limits. A simplified expression is derived. based on the assumption of high inertia. This model is used to obtain a qualitative understanding of how frequency modulation influences the dynamic stability of the variable-reluctance motor.

• Structural Engineering and Mechanics
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• v.13 no.3
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• pp.329-343
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• 2002

In this research, the dynamic stability of an orthotropic elastic conical shell, with elasticity moduli and density varying in the thickness direction, subject to a uniform external pressure which is a power function of time, has been studied. After giving the fundamental relations, the dynamic stability and compatibility equations of a nonhomogeneous elastic orthotropic conical shell, subject to a uniform external pressure, have been derived. Applying Galerkin's method, these equations have been transformed to a pair of time dependent differential equations with variable coefficients. These differential equations are solved using the method given by Sachenkov and Baktieva (1978). Thus, general formulas have been obtained for the dynamic and static critical external pressures and the pertinent wave numbers, critical time, critical pressure impulse and dynamic factor. Finally, carrying out some computations, the effects of the nonhomogeneity, the loading speed, the variation of the semi-vertex angle and the power of time in the external pressure expression on the critical parameters have been studied.

• Structural Engineering and Mechanics
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• v.43 no.1
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• pp.89-103
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• 2012

In this study, the stability of laminated homogeneous and non-homogeneous orthotropic truncated conical shells with freely supported edges under a uniform hydrostatic pressure is investigated. It is assumed that the composite material is orthotropic and the material properties depend only on the thickness coordinate. The basic relations, the modified Donnell type stability and compatibility equations have been obtained for laminated non-homogeneous orthotropic truncated conical shells. Applying Galerkin method to the foregoing equations, the expression for the critical hydrostatic pressure is obtained. The appropriate formulas for the single-layer and laminated, cylindrical and complete conical shells made of homogeneous and non-homogeneous, orthotropic and isotropic materials are found as a special case. Finally, effects of non-homogeneity, number and ordering of layers and variations of shell characteristics on the critical hydrostatic pressure are investigated.

• Journal of Microbiology and Biotechnology
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• v.25 no.6
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• pp.782-787
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• 2015

In this study, we developed an assay system for missense mutations in human phenylalanine hydroxylases (hPAHs). To demonstrate the reliability of the system, eight mutant proteins (F39L, K42I, L48S, I65T, R252Q, L255V, S349L, and R408W) were expressed in a mutant strain (pah^-) of Dictyostelium discoideum Ax2 disrupted in the indigenous gene encoding PAH. The transformed pah - cells grown in FM minimal medium were measured for growth rate and PAH activity to reveal a positive correlation between them. The protein level of hPAH was also determined by western blotting to show the impact of each mutation on protein stability and catalytic activity. The result was highly compatible with the previous ones obtained from other expression systems, suggesting that Dictyostelium is a dependable alternative to other expression systems. Furthermore, we found that both the protein level and activity of S349L and R408W, which were impaired severely in protein stability, were rescued in HL5 nutrient medium. Although the responsible component(s) remains unidentified, this unexpected finding showed an important advantage of our expression system for studying unstable proteins. As an economic and stable cell-based expression system, our development will contribute to mass-screening of pharmacological chaperones for missense PAH mutations as well as to the in-depth characterization of individual mutations.