• 비교문화연구
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• 제39권
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• pp.433-460
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• 2015

This paper was analyzed the politeness and the expression of ending sentence in family conversations in the virtual language of cartoon characters. Younger speakers have a tendency to unite sentence-final particle to the polite form, older speakers have a tendency to unite it to the plain form in the historical genre. But younger speakers and older speakers unite sentence-final particle to the plain form in other fiction genres. Using terms of respect is determined by circumstances and charactonym. Comparing the translation of conversations with the original, there were the different aspects of translated works. When Japanese instructors are used to study Japanese as the instructional media, they give a supplementary explanation to students. 'WA' 'KASIRA' that a female speaker usually uses are used by a male speaker, 'ZO' 'ZE' that a male speaker usually uses are used by a female speaker in the virtual language of cartoons. In the field of the translation, it is translated 'KANA' 'KASIRA' into 'KA?', 'WA' 'ZO' 'ZE' into 'A(EO)?', 'WAYO' 'ZEYO' into AYO(EOYO)'. When we use sentence-final particle in the virtual language of cartoon, we need to supply supplementary explanations and further examinations.

• 한국경영과학회:학술대회논문집
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• 대한산업공학회/한국경영과학회 2000년도 춘계공동학술대회 논문집
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• pp.385-388
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• 2000

The computation of a terminal-pair reliability is started with a Boolean sum of products expression corresponding to simple paths of the pair of nodes. This expression is then transformed into another equivalent expression to be a Sum of Disjoint Products form. The terminal reliability can then be obtained in a straightforward manner from the Sum of Disjoint Products form. In this paper, we proposed an efficient method to obtain reliability expressions for calculating multiple K terminal-pairs reliability between terminal-pairs of nodes of computers of complex networks. Actual programming results show that the new method is superior with regard to computational efficiency, especially for computing the reliability of multiple terminals.

• 한국실내디자인학회:학술대회논문집
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• 한국실내디자인학회 2007년도 춘계학술대회 논문집
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• pp.260-263
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• 2007

A church architecture which has an important meaning as a place which is for worship God, relationship, education, evangelism, service and formative meaning that express as a specific form. It has been changed by age or changes of theology, also its structure and form has been changed so as to change of a rutual. the church architecture that has been authorized as a architecture which is a representation of the age does not represent the age and it has weaknesses for expression of unique identification church has. Also, because it is lacking thoughts about regional viewpoint, it loses the characteristic of regional "landmark" like the past. In addition, a place of worship has been changed materiality of space like auditorium or seats so as to change of consciousness of worship, and it is exposed problems of expression of identification. Therefore the church architecture of twenty first centry needs to holy and be able to deliver their new standards and needs that fit original purpose of church for users.

• 한국통신학회논문지
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• 제33권11A호
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• pp.1053-1062
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• 2008

본 논문은 레일리 페이딩 채널에서 확률밀도함수와 최대비 결합기 출력의 최대모멘트 생성 함수를 일반적이고 간결한 수식적 접근을 통해 보여주었다. 또한, 이를 이용하여 M-PSK, M-PAM, M-QAM의 closed form BER을 증명하였다. 마지막으로 다양한 모의 실험을 통해 증명한 수식이 모의 실험 결과와 정확하게 일치함을 알 수 있다.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2004년도 춘계학술대회 논문집
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• pp.70-73
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• 2004

The approach in this thesis is for prediction of deformed strip profile in hot rolling mill. This approach shows how to make an expression as a mathematical form in predicting strip profile. This approach is based on the velocity field, shear stress and material flow on the strip edge along width direction and lateral displacement and stress along width are analytically calculated. Roll force is calculated in each section and then combined together to show roll force distribution along width. All the assumptions to make equation form for this approach are supported by FEM simulation result and the result of model is verified by FEM result. So, this model will supply very useful tool to the researcher and engineers which takes less time and has similar accuracy in predicting roll force profile comparing to FEM simulation. This model has to be combined with deformed roll profile model, which include thermal crown prediction and wear prediction model to predict deformed strip profile.

• Bulletin of the Korean Chemical Society
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• 제31권9호
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• pp.2607-2612
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• 2010

Human tissue-type plasminogen activator (tPA) is a valuable thrombolytic agent used to successfully treat acute myocardial infarction, thromboembolic stroke, peripheral arterial occlusion, and venous thromboembolism. Recombinant tPA is accumulated as an inactive form in inclusion bodies of E. coli and is refolded in vitro, which is accompanied by extensive aggregation. In the present study, a tPA protease domain was expressed in an active soluble form in the cytosol of E. coli Rosetta-gami cells, which allowed disulfide bond formation and supplied the tRNA molecules required for six rarely used codons in E. coli. This strategy increased the amount of soluble protease domain protein and avoided the cumbersome refolding process. The purified protease domain not only degraded tPA substrate peptides but also formed a covalently bound complex with plasminogen activator inhibitor-1, as does full-length tPA. Soluble expression and purification of tPA domains may aid in functional analyses of this multi-domain protein, which has been implicated in many physiological and pathological processes.

• 한국실내디자인학회논문집
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• 제15권6호
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• pp.120-130
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• 2006

This study is about objectified spatial composition. The Interior Design field has grown and established its major trends dependent upon other related field of study. With this perspective in mind, esthetics on Interior Design has to be treated in similar manner. In modem architects or fine artists have defined the terminology 'objectification' theoretical principle where by unable to distinguish between 'object's type' and 'objects', Presently, the term 'objectified' became a trend word. In order to confront misconception of the idea of 'objectification', some people define as reflection of this complex society. There are five types in expression of objectified spatial composition in Interior Design. Which are free form spatial composition through artistry of artists, fantastic spatial composition by polysemous collision, symbolic spatial composition from the metaphorical of form, aggregate spatial composition by geometric collision and geometric superimposition, and cultural spatial composition. Therefore, this study aims to help understanding of tendency in various space expressions in Interior Design through searching how to express type of objectified spatial composition in Interior Design from 2000 through 2005.

• 복식
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• 제41권
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• pp.23-47
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• 1998

Ornaments is a province of the fashion. It usually express noble metals and jewel's adorn-ment as the symbol of status and riches for a long time. The ornament design of Jewels drawing project and a product by computer are increased development and as exertion as a realization of automation. Through the use of CAD software(Auto CAD R 13 & Jewel CAD) \circled1 A design development of the jewels in industrial society \circled2 A metals art & design on the dress and it's ornaments - Study about application of principle(liberal curve, arrangement of repeated form, gradual unity, rhythmical harmony) Although Auto CAD don't various expression of Jewels than a Jewel CAD, formative.scientific.funtional development of geometrical form is free. That is (to say), geometrical form is given much weigh in the general CAD, but Jewel CAD made concentrate software on the jewels design for the expression of liberal form. The CAD/CAM software for jewellery program is composed of main menu, icons, hotkeys. Changing form is derived from a definite point, curve elements of a drawing. \circled1 3-Dimensional \circled2 Easy and flexible \circled3 Bulit-in and self created library \circled4 From simple wire frame to full color images. As a CAD can practice all the creation activity effectively, from Design & Drafting Software to Rendering generally can present precise results. A point of view of the connection the scientist and art, this practicableness of CAD have a lot of possiblity of development. That will do much for the related fields of industry. Consequently, subjective intension of a creator & humanity with value plays role in practical application of the design.

• Journal of Microbiology and Biotechnology
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• 제7권2호
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• pp.132-137
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• 1997

A novel protein (HEAAE, High Essential Amino Acid Encoding Protein), rich in essential amino acids ($75{\%}$ of total), was designed and constructed in our laboratory. The designed peptides were analyzed by SYBLE and stable secondary and tertiary structures were predicted. The monomeric form (HEAAE-1) of the protein consists of 20 amino acid residues with four additional amino acids comprising a potential ${\beta}$-turn (HEAAE-4). Size exclusion analysis demonstrated that the monomer is self-aggregates in aqueous solution to form higher ordered multimeric structures, which are very reminiscent of natural plant storage proteins. The DNA encoding this amino acid sequence was synthesized, and from this monomeric gene fragment (heaae-1), the stable tetrameric form of the gene (heaae-4) was generated by subcloning into the E. coli expression vector pKK223-3. A clear 6 kDa polypeptide band corresponding to the molecular weight of the dimeric form (HEAAE-2) was detected. The smeared band which appeared around the molecular weight corresponding to HEAAE-4 of 11 kDa suggested that the tetramer form of this protein might be processed into smaller size products.

• Journal of Microbiology and Biotechnology
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• 제14권2호
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• pp.430-434
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• 2004

Three extracellular protease-deficient Bacillus subtilis strains were transformed with the plasmid pCK98 containing the endo-$\beta$-1,4-glucanase (Eng) gene of B. subtilis BSE616. The three transformants, B. subtilis DB104 (pCK98), WB600 (pCK98) and WB700 (pCK98), produced the same high level of enzyme activity and showed similar patterns of cell growth and enzyme production. When B. subtilis DB 104 (pCK98), a two-extracellular protease deficient strain, was cultured for 22 h, almost all the secreted enzyme was found to be in the completely cleaved form by both activity staining and Western blotting studies. B. subtilis WB600 (pCK98), a six-extracellular protease-deficient strain, produced a partially cleaved form in addition to the intact form of the enzyme, although the degree of internal cleavage of the enzyme was greatly reduced. With B. subtilis WB700 (pCK98), a seven-extracellular protease-deficient strain, almost all the enzyme was produced as the intact uncleaved form. This study illustrates that a role of the V pr protease is to degrade foreign proteins produced in B. subtilis and WB700 is a suitable expression system for producing the intact form of the Eng and other foreign proteins that may lose at least part of their efficacy due to internal proteolytic cleavage.