• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.4
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• pp.370-375
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• 2014

BACE2 is a membrane-bound aspartic protease that is highly homologous with BACE1. While BACE1 processes the amyloid precursor protein (APP) at a key step in generating ${\beta}$-amyloid peptide and presumably causes Alzheimer's disease (AD), BACE2 has not been demonstrated to be involved in APP processing directly, and its physiological functions are unknown. To determine its function and to develop inhibitors from marine sources, we constructed an overexpression vector for producing BACE2. The gene encoding human BACE2 protease was amplified using the polymerase chain reaction and cloned into the pET11a expression vector, resulting in pET11a/BACE2. Recombinant BACE2 protease was overexpressed successfully in E. coli as inclusion bodies, refolded using the rapid-dilution method, and purified via two-step fast protein liquid chromatography using Sephacryl S-300 gel filtration and Resource-Q column chromatography. The BACE2 protease produced was an active form. This study provides an efficient method not only for studying the basic properties of BACE2, but also for developing inhibitors from natural marine sources.

• The Korean Journal of Physiology and Pharmacology
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• v.17 no.5
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• pp.427-433
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• 2013

Receptor activator of NF-${\kappa}B$ ligand (RANKL)-induced osteoclastogenesis is accompanied by intracellular $Ca^{2+}$ mobilization in a form of oscillations, which plays essential roles by activating sequentially $Ca^{2+}$/calmodulin-dependent protein kinase, calcineurin and NFATc1, necessary in the osteoclast differentiation. However, it is not known whether $Ca^{2+}$ mobilization which is evoked in RANKL-independent way induces to differentiate into osteoclasts. In present study, we investigated $Ca^{2+}$ mobilization induced by aluminum fluoride ($AlF_4^-$), a G-protein activator, with or without RANKL and the effects of $AlF_4^-$ on the osteoclastogenesis in primary cultured mouse bone marrow-derived macrophages (BMMs). We show here that $AlF_4^-$ induces intracellular $Ca^{2+}$ concentration ($[Ca^{2+}]_i$) oscillations, which is dependent on extracellular $Ca^{2+}$ influx. Notably, co-stimulation of $AlF_4^-$ with RANKL resulted in enhanced NFATc1 expression and formation of tartrate-resistant acid phosphatase (TRAP) positive multinucleated cells. Additionally, we confirmed that mitogen-activated protein kinase (MAPK) is also activated by $AlF_4^-$. Taken together, these results demonstrate that G-protein would be a novel modulator responsible for $[Ca^{2+}]_i$ oscillations and MAPK activation which lead to enhancement of RANKL-mediated osteoclastogenesis.

• Journal of KIISE:Databases
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• v.35 no.3
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• pp.243-256
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• 2008

With rapid growth of Internet, the amount of information in the Web is increasing exponentially. However, information on the current Web is understandable only for human, and thus it makes the exact information retrieval difficult. For solving this problem, the Semantic Web is suggested and we must use ontology languages that can endow data to semantics for implementing it. One of the representative ontology languages is OWL(Web Ontology Language) adopted as a recommendation by the World-Wide Web Consortium. OWL has richer expression power and formal semantics than other ontology languages such as RDF and RDF-S. In addition, OWL includes hierarchical structure information between classes or properties. Therefore, an efficient OWL storage model considering hierarchical structure for effective information retrieval on the Semantic Web is required. In this paper, we suggest the XPOS(XPath-based OWL Storage) model including hierarchy information between classes or properties as XPath form and enabling intuitive and effective information retrieval. Also, we show the comparative evaluation results on the performance of XPOS model, Sesame, and the XML storage-based storage model regarding query processing.

• CELLMED
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• v.6 no.4
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• pp.26.1-26.3
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• 2016

The purpose of this article is to argue that Korean music, Yukjabaegi (six-bit songs), helps us overcome grief. It is not untrue to say that Yukjabaegi is among the greatest types of music in Korea. This is a testament to the prominence of this statue among Han Ak (Korean music, 韓樂). Empathy is an inherent human quality involving all parts of the brain which is also linked to feelings of love. Empathy is created the moment we imagine ourselves in someone else's situation. Moreover, if empathy is good for us, the generation of empathy may actually mobilize our strength. Koreans have superior strength in overcoming grief and difficult situations by singing together. Often Koreans feel that their lives and labors are difficult, they start singing Yukjabaegi because they consider grief and anxiety wear on a person more than hard work. Listening to and singing Yukjabaegi can cheer up people who feel as limp as a rag after hard labor. Tackling reality and venting the experience via creative expression is bound to be healthy. Under a change of the rhythm pattern, Yukjabaegi thus becomes a major form of amusement for those suffering from difficult situations and comes as a true friend to share in our grief with us. Singing together via Yukjabaegi is a time to share grief. Thus, this, author believes that it is related to empathy. It helps us overcome grief by means of music therapy.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.6
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• pp.2447-2451
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• 2014

Human papillomavirus (HPV) is the primary etiologic agent of cervical cancer. Consideration of safety and non human leukocyte antigen restriction, protein vaccine has become the most likely form of HPV therapeutic vaccine, although none have so far been reported as effective. Since tumor cells consistently express the two proteins E6 and E7, most therapeutic vaccines target one or both of them. In this study, we fabricated DC vaccines by transducing replication-defective recombinant adenoviruses expressing E6/E7 fusion gene of HPV-16, to investigate the lethal effects of specific cytotoxic T lymphocytes (CTL) against CaSki cells in vitro. Mouse immature dendritic cells (DC) were generated from bone marrow, and transfected with pAd-E6/E7 to prepare a DC vaccine and to induce specific CTL. The surface expression of CD40, CD68, MHC II and CD11c was assessed by flow cytometry (FCM), and the lethal effects of CTL against CaSki cells were determined by DAPI, FCM and CCK-8 methods. Immature mouse DC was successfully transfected by pAd-E6/E7 in vitro, and the transfecting efficiency was 40%-50%. A DC vaccine was successfully prepared and was used to induce specific CTL. Experimental results showed that the percentage of apoptosis and killing rate of CaSki cells were significantly increased by coculturing with the specific CTL (p <0.05). These results illustrated that a DC vaccine modified by HPV-16 E6/E7 gene can induce apoptosis of CaSki cells by inducing CTL, which may be used as a new strategy for biological treatment of cervical cancer.

• Korean Journal of Psychosomatic Medicine
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• v.12 no.2
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• pp.103-121
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• 2004

Conversion disorder and factitious disorder should be ruled out before making diagnosis of malingering. For this work, inspection of patient's behavior along with complete neurological examinations, psychological tests, and meticulous psychiatric interview are necessary. Facial expression test, thermography, dynamometry were failed differentiating conversion disorders to the malingered pain and motor symptoms, however, controlled diagnostic block showed positive result partly in patients with regional or cervical pain syndrome. Chronic pain patients who are related to the process of litigation encounter stressful life situations which lead them into various neurobehavioral and psychosocial complications. Most of suspected malingered patients would not revealed pure form rather mixed with factitious and/or conversion features. At the time of increasing number of chronic patients associated with traffic accidents or industrial injuries who are involved in litigation, psychiatrist are needed sharp eyes and tenacity for evaluating malingering or medically unexplained symptoms.

• Journal of Microbiology and Biotechnology
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• v.25 no.6
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• pp.936-940
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• 2015

Human papillomavirus (HPV) type 52 is a high-risk HPV responsible for cervical cancer. HPV type 52 is common around the world and is the most common in some Asian regions. The available prophylactic HPV vaccines protect only from HPV types 16 and 18. Supplementing economical vaccines that target HPV type 52 may satisfactorily complement available prophylactic vaccines. A codon-adapted HPV 52 L1 gene was expressed in the methylotrophic yeast Hansenula polymorpha, which is used as an industrial platform for economical hepatitis B surface antigen particle production in China. We found that the recombinant proteins produced in this expression system could form virus-like particles (VLPs) with diameters of approximately 50 nm. This study suggests that the HPV 52 VLPs produced in this platform may satisfactorily complement available prophylactic vaccines in fighting against HPVs prevalent in Asia.

• Journal of Biomedical Engineering Research
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• v.38 no.3
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• pp.123-128
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• 2017

Background and aims: The KCNQ1 S140G mutation involved in $I_{ks}$ channel is a typical gene mutation affecting atrial fibrillation. However, despite the possibility that the S140G gene mutation may affect not only atrial but also ventricular action potential shape and ventricular responses, there is a lack of research on the relationship between this mutation and ventricular fibrillation. Therefore, in this study, we analyzed the correlation and the influence of the KCNQ1 S140G mutant gene on ventricular fibrillation through computer simulation studies. Method: This study simulated a 3-dimensional ventricular model of the wild type(WT) and the S140G mutant conditions. It was performed by dividing into normal sinus rhythm simulation and reentrant wave propagation simulation. For the sinus rhythm, a ventricular model with Purkinje fiber was used. For the reentrant propagation simulation, a ventricular model was used to confirm the occurrence of spiral wave using S1-S2 protocol. Results: The result showed that 41% shortening of action potential duration(APD) was observed due to augmented $I_{ks}$ current in S140G mutation group. The shortened APD contributed to reduce wavelength 39% in sinus rhythm simulation. The shortened wavelength in cardiac tissue allowed re-entrant circuits to form and increased the probability of sustaining ventricular fibrillation, while ventricular electrical propagation with normal wavelength(20.8 cm in wild type) are unlikely to initiate re-entry. Conclusion: In conclusion, KCNQ1 S140G mutation can reduce the threshold of the re-entrant wave substrate in ventricular cells, increasing the spatial vulnerability of tissue and the sensitivity of the fibrillation. That is, S140G mutation can induce ventricular fibrillation easily. It means that S140G mutant can increase the risk of arrhythmias such as cardiac arrest due to heart failure.

• Journal of Korean Medical classics
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• v.33 no.1
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• pp.135-147
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• 2020

Objectives : This study focuses on the condition Yinynagjiao, which was first mentioned in 『Huangdineijing』, on the cause, mechanism and reason for it being described as fatal. Later doctors's understanding and treatment of this condition were examined subsequently. Methods : Verses related to Yinyangjiao from texts such as 『Huangdineijing』, 『Jinkuiyuhanjing』, 『Maijing』 were cross-examined focusing on their context and difference in expression with reference to annotations and later texts that mention Yinyangjiao. Based on the findings, its mechanism and treatment methods as found in Wenbing texts were compared with descriptions from previous texts. Results & Conclusions : According to 『Huangdineijing』, heat disease belongs to the Shanghan category, treated through promoting perspiration. In the confrontational position between JingQi and XieQi, perspiration could be understood as the result of JingQi's victory that lead to the discharge of XieQi in the form of sweat. Yinyangjiao is the opposite situation where Jing is discharged while heat toxin sinks into the body, thus being fatal, and treatment aimed for perspiration not adequately addressing the main problem which was ultimately expected to resolve through means of solid Yin Jing that would stabilize the pulse in due time. On the other hand, Wenbing scholars saw the pathogen as heat, shifting previous perspectives entirely. As a result, instead of applying pungent/warm medicinals to promote sweating, they suggested the use of cool, sweet/moist medicinals to cool the heat and nourish Yin as means of treating the condition.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.9 no.7
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• pp.1432-1438
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• 2005

The RFID (Radio Frequency Identification) technology is a non-contact identification system which can wireless reading of information, by attaching tags on objects; various applications of the technology are feasible throughout a dive of industries, and researches are currently under way. Recently, in connection with the wireless communication industry, application service models conveying information to the users through mobile devices with RFID readers were suggested. Such application service based on mobile RFID technology requires schema that models the form of data. Therefore, this study analyzes the mobile RFID application service model that will be serviced in the mobile environment, functions and elements of the protocol, and the interface, designing a language to process the data and express contents in the said service models, and we simulated the service for test it.