• Korean Institute of Interior Design Journal
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• v.23 no.3
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• pp.222-230
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• 2014

Modern museums introduce a so-called method of 'exhibitions that approach' apart from the scheme of exhibitions in a simple listing type, creating a series of stories based on the original forms of remains put on display, adopting various methods of media access, and enabling the spectators of the museums to find exhibits a little more convincing and understand them more in depth, which might look somewhat isolated from their everyday lives. The configuration of the exhibitions that approach can easily be found in the mode of narrative development of exhibition topics and scenarios, and in this sense, a study on the narrativity of exhibitions is effective for analyzing the exhibition spaces of the museums. Furthermore, an analysis on exhibition spaces may be conducted through the process of forming the messages of exhibition contents and interpreting the narrative structures of the modes of development, and allows people to think that the methods of interpreting the spaces established like this may form an organic complementary relationship with exhibition contents and have a more extended meaning. Thus, this study examines the narrativity of Jeon-gok prehistory museum and the narrative structure systems based on the structuralist narrative theory, approaches the modes of narrative development of the spaces based on semiotic judgment, and aims to understand the structures of the space narrative. In addition, It is another object of the present invention in order to verify the objectivity, throughout the course of additional case studies, to improve the efficiency of future exhibition design.

• Korean Institute of Interior Design Journal
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• v.23 no.3
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• pp.48-55
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• 2014

Luis Barragan is a representative Mexican architect. He designed architectural space as a local architect in consideration of the Mexico's harsh Mediterranean climate. His manifestation of the regionality can be found mainly in his precise expression of light. Such method and concept of using light in Barragan's architecture made a huge impact on later architects but detailed studies on this issue have not been made actively. Hence, this paper intended to suggest a starting point or an orientation by illustrating the characteristics of using and expressing light in architecture through his cases of housing works. The study method was as follows. First, the general characteristics of light were identified by analyzing the elements of architectural space that dealt with light and the characteristics of expressing light shown in the architectural space. Second, the architectural background of Luis Barragan and the architectural space elements of the light expressed in his architectural space were extracted to form a hypothetical ground. Third, five of his works on the houses in his prime (after the year of 1945) were analyzed to explore the characteristics of relational representation between light and the architectural space elements in his architecture. Through this process, it was learned that, in his architectural works, light was the embodiment of the location-related, time-based, daily and illusionary experience into architecture. In addition, meditative light could be experienced by contrasting the soft and dispersed light and shadow of the inside space of Barragan's houses with the strong external light. Therefore, through the process above, this study is hoped to provide an opportunity for the contemporary architects of our era to rethink the fundamental correlation between architecture and light.

• Korean Institute of Interior Design Journal
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• v.22 no.2
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• pp.68-80
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• 2013

This study is about expressive aspects of subversive mimesis found in architectural design under 2nd Modernity. Architectural works under 2nd Modernity are described based on pseudo-scientific positivism and philosophical ontology of Deleuze. However, subversiveness found in works of arts by architects such as Rem Koolhaas present a few complexities to relay on such a description. This is about 'subversiveness' which absorbs the positive and negative factors of modernity which has been multi-layered as 'cultural capital'. This study aims to identify meta-phenomenon as well as the specific correlations between expression and purposes of any work of art that is presented in the form of subversiveness. To achieve this aim, this study approached with the concepts of appropriation and detournment based on Adorno's subversive mimesis concept. Meta-phenomena of architectural design methods occur from relations of three, which are social reality, artist, and work of art. This was connected to productivity of mimesis practice of self-reference and self-examination, which was then, summed up from the perspectives of appropriation in pure arts and d$\acute{e}$tournment of situationalists. Based on this work, subversive expressive characteristics of architecture under 2nd Modernity were framed from the perspectives of the absorption of cultural capital, reflection and negation, autonomy, instrumentality, and meta properties. In this way, this study found that Adorno's subversiveness should be effective for creative and methodological systemization in terms of interpretation of cognition, practice, and effect after materialization.

• The Journal of the Korea Contents Association
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• v.8 no.6
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• pp.121-129
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• 2008

With the help of information technology The problem of building a concrete form with a shapeless media is the starting point of presenting information by means of sound. Audio-Visual System using sound as a medium is a method that presents an auditory material visually and has a role of linking different sensory organs. In a sense, Audio-Visual System transfers a non-concrete sensation to a concrete one. Audio-Visual System presentation by late methods using active and non-active images produced by computerized irregular procedures can be limited because of the limited ways of visual output. On the other side, visualization using active images can induce more diverse expressions using sound as a medium. This study suggests a new way of expression in animations using visualization of various auditory materials and sounds built by Audio-Visual System with active images.

• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.563-569
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• 2004

The DNA sequence of the chitosanase gene (choK) from $\beta$-Proteobacterium KNU3 showed an 1,158-bp open reading frame that encodes a protein of 386 amino acids with a novel 74 signal peptide. The degenerated primers based on the partial deduced amino acid sequences from MALDI- TOF MS analyses yielded the 820 bp of the PCR product. Based on this information, double inverse PCR cloning experiments, which use the two specific sets of PCR primers rather than single set primers, identified the unknown 1.2 kb of the choK gene. Subsequently, a 1.8 kb of full choK gene was cloned from another PCR cloning experiment and it was then subcloned into pGEM T-easy and pUC18 vectors. The recombinant E. coli clone harboring recombinant pUC18 vector produced a clear halo around the colony in the glycol chitosan plates. The recombinant ChoK protein was secreted into medium in a mature form while the intracellular ChoK was produced without signal peptide cleavage. The activity staining of PAGE showed that the recombinant ChoK protein was identical to the chitosanase of wild-type. The comparison of deduced amino acid sequences of choK revealed that there is 92% identity with that of Sphingobacterium multivorum chitosanase. Judging from the conserved module in other bacterial chitosanases, chitosanase of KNU3 strain (ChoK) belongs to the family 80 of glycoside hydrolases.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.11
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• pp.1565-1572
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• 2015

Porcine embryonic stem cells (pESCs) have become an advantageous experimental tool for developing therapeutic applications and producing transgenic animals. However, despite numerous reports of putative pESC lines, deriving validated pESC lines from embryos produced in vitro remains difficult. Here, we report that embryo aggregation was useful for deriving pESCs from in vitro-produced embryos. Blastocysts derived from embryo aggregation formed a larger number of colonies and maintained cell culture stability. Our derived cell lines demonstrated expression of pluripotent markers (alkaline phosphatase, Oct4, Sox2, and Nanog), an ability to form embryoid bodies, and the capacity to differentiate into the three germ layers. A cytogenetic analysis of these cells revealed that all lines derived from aggregated blastocysts had normal female and male karyotypes. These results demonstrate that embryo aggregation could be a useful technique to improve the efficiency of deriving ESCs from in vitro-fertilized pig embryos, studying early development, and deriving pluripotent ESCs in vitro in other mammals.

• Journal of Microbiology and Biotechnology
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• v.2 no.4
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• pp.243-247
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• 1992

The thr operon of Escherichia coli TF427, an $\alpha$-amino-$\beta$-hydroxyvaleric acid (AHV)-resistant threonine overproducer, was cloned in a pBluescriptII $KS^＋$ plasmid by complementation of E. coli mutants. All clones contained a common 8.8 kb HindIII-generated DNA fragment and complemented the thrA, thrB, and thrC mutants by showing that these clones contained the whole thr operon. This thr operon was subcloned in the plasmid vectors pBR322, pUC18, and pECCG117, an E. coli/Corynebacterium glutamicum shuttle vector, to form recombinant plasmids pBTF11, pUTF25 and pGTF18, respectively. The subcloned thr operon was shown to be present in a 6.0 kb insert. A transformant of E. coli TF125 with pBTF11 showed an 8~11 fold higher aspartokinase I activity, and 15~20 fold higher L-threonine production than TF125, an AHV-sensitive methionine auxotroph. Also, it was found that the aspartokinase I activity of E. coli TF125 harboring pBTF11 was not inhibited by threonine and its synthesis was not repressed by threonine plus isoleucine.

• Journal of Microbiology and Biotechnology
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• v.23 no.8
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• pp.1107-1115
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• 2013

The Cyt1Aa protein of Bacillus thuringiensis susbp. israelensis elaborates demonstrable toxicity to mosquito larvae, but more importantly, it enhances the larvicidal activity of this species Cry proteins (Cry11Aa, Cry4Aa, and Cry4Ba) and delays the phenotypic expression of resistance to these that has evolved in Culex quinquefasciatus. It is also known that Cyt1Aa, which is highly lipophilic, synergizes Cry11Aa by functioning as a surrogate membrane-bound receptor for the latter protein. Little is known, however, about whether Cyt1Aa can interact similarly with other Cry proteins not primarily mosquitocidal; for example, Cry2Aa, which is active against lepidopteran larvae, but essentially inactive or has very low toxicity to mosquito larvae. Here we demonstrate by ligand binding and enzyme-linked immunosorbent assays that Cyt1Aa and Cry2Aa form intermolecular complexes in vitro, and in addition show that Cyt1Aa facilitates binding of Cry2Aa throughout the midgut of C. quinquefasciatus larvae. As Cry2Aa and Cry11Aa share structural similarity in domain II, the interaction between Cyt1Aa and Cry2Aa could be a result of a similar mechanism previously proposed for Cry11Aa and Cyt1Aa. Finally, despite the observed interaction between Cry2Aa and Cyt1Aa, only a 2-fold enhancement in toxicity resulted against C. quinquefasciatus. Regardless, our results suggest that Cry2Aa could be a useful component of mosquitocidal endotoxin complements being developed for recombinant strains of B. thuringiensis subsp. israelensis and B. sphaericus aimed at improving the efficacy of commercial products and avoiding resistance.

• The Journal of the Korea Contents Association
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• v.7 no.4
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• pp.141-149
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• 2007

In this study, the use of abstraction has been considered, focusing on the abstract metaphoric expressions presented in Animatrix and it has a purpose as the case study in the use of abstraction as a unique element of animation in which imaginative ideas and stimuli are significantly important and the pioneering works of abstract animations were produced in the early 20th century. The abstract metaphors expressed in The Second Renaissance Part1 & Part2 and Matriculated among the episodes of Animatrix, the animation produced in the omnibus form directed by Wachowski Brothers and great Master in the world, can be analyzed into Anima of Abstraction abstractively expressing organic vital power, Extensity of Abstraction which 4D space is realized possibly recognizing the human emotions as well as 3D space, and Conceptualization of Abstraction directly presenting the human mentality and imaginary world.

• The Journal of the Korea Contents Association
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• v.7 no.1
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• pp.207-214
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• 2007

Symbol mark started with expressing the symbolic form through the long history of human beings, in order to transmit self or group's life and thoughts, and since the Industrial Revolution, symbol mark has beer used for the business activities of enterprise, along with the acquirement of term 'CI'. The symbol mark design of A.E.G, designed by Peter Behrens in 1907 is the onset of symbol mark in the CI concept, and the concept of CI and symbol mark was introduced to our nation in the early of 1970s. Since then, until now, CI has reflected the phase of the times as a key factor of CI, while repeating numerous changes, and recently, it is a state that the expression methods of symbol mark have been varied with the popularization of trend, appealing to emotion in the whole society and culture. Accordingly, this thesis examines the corporate symbol mark through the portfolio of representative CI specializes companies from the introduction period of CI to the present, and analyze the features of the time in order to present the basis of the direction of symbol mark design for the creation of future corporate images.