• 제어로봇시스템학회논문지
• /
• 제12권2호
• /
• pp.130-137
• /
• 2006

In this paper, we propose a method of selecting new types of rectangle features that are suitable for facial expression recognition. The basic concept in this paper is similar to Viola's approach, which is used for face detection. Instead of previous Haar-like features we choose rectangle features for facial expression recognition among all possible rectangle types in a 3${\times}$3 matrix form using the AdaBoost algorithm. The facial expression recognition system constituted with the proposed rectangle features is also compared to that with previous rectangle features with regard to its capacity. The simulation and experimental results show that the proposed approach has better performance in facial expression recognition.

• Journal of Microbiology
• /
• 제40권1호
• /
• pp.77-81
• /
• 2002

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a pleiotropic hematspoietic growth factor involved in the development of myeloid cells from bone marrow, and an activator of mature myeloid cells functioning in a variety of antimicrobial and inflammatory responses. Recently, recombinant GM-CSF is increasingly under clinical study for treatment of various diseases including cancer, infectious diseases and hematopoietic diseases as well as for an immune response modulator, In this study, we constructed a recombinant human GM-CSF (rhGM-CSF) expression plasmid with a pelB leader sequence and His. Tag under T7 promoter control. The expression construct was shown to produce a recombinant protein of 20 kDa in the 8M urea preparation, indicating the rhGM-CSF may be expressed as an insoluble inclusion form. The 20 kDa recombinant protein in 8M urea was transformed into the water-so1ub1e form by dialysis against PBS buffer (phosphate buffered saline). The soluble rhGM-CSF protein was shown to stimulate colony formation and cell proliferation in vitro, indicating that the rhGM-CSF could be refolded into its native form to show colony stimulating activity.

• 한국콘텐츠학회:학술대회논문집
• /
• 한국콘텐츠학회 2007년도 추계 종합학술대회 논문집
• /
• pp.657-660
• /
• 2007

2000년대에 들어서면서 우리나라 만화시장은 웹 만화의 성장세가 매우 두드러지고 있다. 이 가운데 스크롤만화는 웹 만화의 주류 표현 양식으로 자리 잡으면서 기존의 출판만화가 갖고 있던 형식을 벗어나 독자에게 새로운 시각적 체험을 선사하고 있다. 특히 세로로 긴 스크롤 방식은 출판만화의 칸 틀과는 비교할 수 없는 '장대한 세로칸'의 사용, 애니메이션적인 기법의 활용, 혁신적인 칸나누기, 말풍선과 나레이션의 칸 밖 배치 등 디지털 만화가 가진 '무한 캔버스'와 웹페이지의 '스크롤 바'를 충분히 활용하는 방식으로 디지털만화시대를 이끌었다. 그러나 '스크롤만화'는 새롭게 도래하고 있는 웹2.0시대에도 여전히 유효할지는 미지수다. 디지털과 웹의 무한한 가능성에도 불구하고 웹 만화가 '스크롤'이라는 하나의 창작 방식에만 너무 매몰되어 있는 오늘의 모습은 우려를 낳기에 충분하다. 본고에서는 스크롤만화를 중심으로 한 웹 만화의 표현양식을 분석해 보고 이를 토대로 향후 디지털만화가 진화해갈 방향을 점검해 보고자 한다.

• 건축역사연구
• /
• 제32권4호
• /
• pp.19-33
• /
• 2023

This study investigated the architectural expression of Taekpungdang(澤風堂, The Pond and Wind House) built by the Neo-Confucianist Taekdang Lee Sik(澤堂 李植, 1584~1647) from the perspective of the symbolic system of the Zhōuyì(『周易』, Classic of Changes). This study examined the historical context, personal history, and construction process of Taekpungdang at the time of its creation through his collection of writings, the Taekdanggip(澤堂集). The study also estimated the original form of Taekpungdang through field surveys and historical evidence. In addition, the architectural principles and architectural expressions inherent in the Taekpungdang were derived based on the symbolic system of "taekpungdaegwa"(澤風大過) which is Lee Sik's divination and one of the 64 trigrams in the Zhōuyì. Lee Sik, who was knowledgeable in the Zhōuyì, used divination to cope with the chaotic political situation and his own misfortunes. Accordingly, He determined the direction of his life and planned the surrounding environment, architectural structure, and form of Taekpungdang based on the rules and meanings of his divination system. He embodied the architectural space of Taekpungdang with the concept of time and space inherent in the divination of "daegwa",(大過, great exceeding). In addition, he expressed the principle of the generation of palgue,(八卦, the eight trigrams for divination) and the principle of the co-prosperity of ohaeng(五行, the five elements) through the composition of walls and windows of the house. The images of Taekpungdaegwae, which are dongyo(棟撓 wood submerged in the pond) and taekmyeolmok(澤滅木, shaking pillars), were manifested in the form of buildings. Therefore, Taekpungdang can be considered a remarkable example of a building designed through the thorough utilization of the Zhōuyì divination system.

• 한국컴퓨터산업학회논문지
• /
• 제8권4호
• /
• pp.229-236
• /
• 2007

분해식은 SOP 형태의 논리식들이 논리합과 논리곱으로 반복해서 표현된 논리식이다. 분해식을 산출하는 과정은 논리식 내에 있는 공통식을 찾아 인수분해를 반복하는 과정이다. 분해식의 형태에 따라 대수 분해식과 부울 분해식으로 구분되며, 리터럴 개수를 기준으로 부울 분해식이 대수 분해식보다 간략화된 형태를 갖는다. 본 논문은 부울 분해식 산출 방법을 제안한 것이다. 제안하는 방법은 주어진 논리식에서 2개의 큐브를 선택하여 제수/몫 쌍들을 산출한다. 이 때, 2개의 큐브로 구성된 몫에 공통인수를 남겨두어 확장 제수/몫 쌍들을 산출하고 후에 몫/몫 쌍들을 산출하도록 하였다. 산출된 제수/몫 쌍과 확장 제수/몫 쌍, 몫/몫 쌍들을 이용하여 부울 분해식 산출 을 위한 행렬을 산출하고, 행렬 커버링을 통해 부울 분해식을 산출하는 방법을 제시한다.

• Molecules and Cells
• /
• 제39권7호
• /
• pp.536-542
• /
• 2016

Interleukin-17A is a member of the IL-17 family, and is known as CTLA8 in the mouse. It is produced by T lymphocytes and NK cells and has proinflammatory roles, inducing cytokine and chemokine production. However, its role in tumor biology remains controversial. We investigated the effects of locally produced IL-17A by transferring the gene encoding it into CT26 colon cancer cells, either in a secretory or a membrane-bound form. Expression of the membrane-bound form on CT26 cells dramatically enhanced their proliferation in vitro. The enhanced growth was shown to be due to an increased rate of cell cycle progression: after synchronizing cells by adding and withdrawing colcemid, the rate of cell cycle progression in the cells expressing the membrane-bound form of IL-17A was much faster than that of the control cells. Both secretory and membrane-bound IL-17A induced the expression of Sca-1 in the cancer cells. When tumor clones were grafted into syngeneic BALB/c mice, the tumor clones expressing the membrane-bound form IL-17A grew rapidly; those expressing the secretory form also grew faster than the wild type CT26 cells, but slower than the clones expressing the membrane-bound form. These results indicate that IL-17A promotes tumorigenicity by enhancing cell cycle progression. This finding should be considered in treating tumors and immune-related diseases.

• 한국어학
• /
• 제48권
• /
• pp.333-354
• /
• 2010

When defining the word related to passive and causative in Korean dictionary, the meaning of headword can be explained by linking them to other related words. The link could be expressed into two forms; the one is 'passive verb causative verb of A' and the other is 'passive form causative form of A.' Whichever the dictionary takes, the important thing is that the content to which it refer should be correct. However the format of 'passive verb causative verb of A' and 'passive form causative form of A' is problematic because the definition of headword does not contain semantic information but syntactic or morphological information. Generic concept 'passive form causative form' and 'passive verb causative verb' refers to respectively morphological and syntactic level but specific concept 'A' refers to semantic level. These morphological, syntactic and semantic level can not be a same dimension so the size of their denotation can not be compared. The way of transform syntactic dimension 'passive verb causative verb' and morphological dimension 'passive form causative form' into semantic dimension is removing 'verb' and 'form' from 'passive verb causative verb' and 'passive form causative form' respectively. Therefore the expression 'passive verb causative verb of A' or 'passive form causative form of A' ought be changed into 'passive causative of A.'

• 복식문화연구
• /
• 제14권1호
• /
• pp.128-141
• /
• 2006

The purposes of this study are to consider characteristics of 'movement expression' that are not discovered in preceeding design studies in order to suggest 'movement expression' as a design formative principal and to make clothes which follows this idea. For the method of this study I used various literature about 'movement expression' as theoretical background and analyzed modern clothing in which 'movement expression' is expressed by collecting magazines including foreign collections from 1989 to 2004 and searching the internet. The results obtained from this study is as follows. First, clothing in which 'movement expression' is expressed removes monotony and tedium of clothing design, provides visual interest and satisfaction for clothing, and delivers designers' concept effectively by attracting a gaze quickly. And It is suggested a new division of 'movement expression' through analysis of the 'movement expression' expressed in clothing. It included the intentional movement by wearers, which is the 'movement expression' of broader meaning such as changes of form or position by a certain operation and changes of existence or function. This movement is not fixed and repeated but changes continuously. As a result of including the design expressing the intentional movement by wearers, one's own personality can be expressed more effectively. It is presented a design which has changeable movement expression continuously.

• 대한의생명과학회지
• /
• 제26권4호
• /
• pp.256-266
• /
• 2020

Carboxypeptidase D (CPD) is a zinc-dependent protease, which is highly expressed in macrophages, and is thought to participate in inflammatory processes. In the present study, we investigated the possible regulatory effect of all-trans retinoic acid (ATRA), which is an active form of vitamin A and plays a critical regulatory role in both the innate and adaptive immunity, on CPD expression and secretion in human monocytic THP-1 cells. CPD mRNA expression first increased, from a concentration as low as 10 nM ATRA to a maximum level of expression, at 1 μM. ATRA enhanced intracellular CPD expression in a time- and concentration-dependent manner but did not affect cell surface CPD expression. Interestingly, 9-cis-RA did not affect CPD expression. Additionally, an experiment with RAR/RXR selective agonist or antagonists demonstrated that ATRA-induced enhancement of CPD expression was RAR/RXR dependent. ATRA also enhanced CPD secretion from THP-1 cells; however, this enhancement was RAR/RXR-independent. The anti-inflammatory agent dexamethasone reversed ATRA-induced enhancement of CPD expression and secretion. Our results suggest ATRA exerts regulatory effects on expression and secretion of CPD in human monocytes, and ATRA-induced CPD secretion may be associated with inflammatory response.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권12호
• /
• pp.7523-7527
• /
• 2013

Thymidylate synthase (TS) catalyzes the transfer of a methyl group from methylenetetrahydrofolate to dUMP to form dTMP. It is a primary target in the chemotherapy of colorectal cancers and some other neoplasms. In order to obtain pure protein for analysis of structure and biological function, an expression vector TS-pET28b (+) was constructed by inserting wild-type human thymidylate synthase (hTS) cDNA into pET28b (+). Then an expression strain was selected after transformation of the recombined plasmid into Rosetta (DE3). Fusion protein with His-tag was efficiently expressed in the form of inclusion bodies after IPTG induction and the content was approximately 40.0% of total bacteria proteins after optimizing expression conditions. When inclusion bodies were washed, dissolved and purified by Ni-NTA under denatured conditions, the purity was up to 90%. On SDS-PAGE and West-blotting, the protein band was found to match well with the predicted relative molecular mass-36kDa. Bioactivity was 0.1 U/mg. The results indicated that high-level expression of wild-type hTS cDNA can be achieved in prokaryotes with our novel method, facilitating research into related chemotherapy.