• 한국식물병리학회지
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• 제14권1호
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• pp.13-18
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• 1998

The use of bioluminescence as a sensitive marker for the detection of Pseudomnas sp. in the rhizosphere was investigated. Transposon Tn4431 which contains a promoterless luciferase operon and tetracycline resistant gene was used. This transposon, present on a suicide vector (pUCD623) in E. coli HB101, was mated with spontaneous rifampicin mutant of Pseudomonas fluorescens B16, a plant growth promoting rhizobacteria (PGPR), and then rifampicin and tetracycline resistant survivors were isolated. Twenty tow mutants wer isolated from the conjugants between E. coli HB101 and P. fluorescens B16. One of these, B16::Tn4431 (L22) recombinant which glowed brightly in the dark was selected for analysis. The cucumber seeds inoculated with L22 were grown in moisten two layers of filter paper and nonsterile soil contained in half cut PVC pipe. The roots were removed from the filter paper and PVC pipe, then placed on the 1/2 LB media plates. The plates were incubated at room temperature for 16 hr. L22 could successfully be detected in the rhizoplane by using the ordinary negative camera film (ASA100-400) with 30 minutes exposure under dark condition. The root colonizing ability and the plant growth promoting effect of L22 were not reduced compared to the untreated bacteria and wild type. L22 was superior to will type.

• 대한임상독성학회지
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• 제2권2호
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• pp.67-71
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• 2004

Arsenic poisoning has three types of poisoning. First, acute arsenic poisoning is usually caused by oral intake of large amount of arsenic compound with purpose of homicide or suicide. Second, chronic arsenic poisoning is caused by inhalation of arsenic in the occupational setting or by long-term oral intake of arsenic-contaminated well water. Third, arsine poisoning occurs acutely when impurities of arsenic in non-ferrous metal react with acid. Clinical manifestation of acute arsenic poisoning is mainly gastrointestinal symptoms and cardiovascular collapse. Those of chronic poisoning are skin disorder and cancer. Arsine poisoning shows massive intravascular hemolysis and hemoglobinuria with acute renal failure. Exposure evaluation is done by analysis of arsenic in urine, blood, hair and nail. Species analysis of arsenic is very important to evaluate inorganic arsenic acid and mono methyl arsenic acid (MMA) separated from dimethyl arsenic acid (DMA) and trimethyl arsenic acid (TMA) which originate from sea weed and sea food. Treatment with dimercaprol (BAL) is effective in acute arsenic poisoning only.

• 대한임상독성학회지
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• 제19권2호
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• pp.127-132
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• 2021

Purpose: It is known that the most common cause of gas poisoning in Korea is suicide attempts by burning ignition coals. Ignition coals are made from waste wood, and studies have been reported that heavy metals are emitted when this coal is burned. However, there was no study on how much heavy metal poisoning occurs in the human body through this, so this study was planned to find out whether the concentration of heavy metals in the blood increased in patients exposed to ignition coal combustion. Methods: From April 2020 to April 2021, blood lead, mercury, and cadmium concentrations were investigated in carbon monoxide poisoning patients who visited one regional emergency medical center in Seoul, and their association with exposure time, source of poisoning, and rhabdomyolysis were investigated. Results: During the study period, a total of 136 carbon monoxide poisoning patients were tested for heavy metals, and 81 cases of poisoning by ignition coal were reported. When comparing poisoning caused by combustion of ignition coal and other substances, there was no difference in the concentrations of lead, mercury, and cadmium in the blood, and there was no difference in the number of patients above the reference range. However, the patients exposed to more than 5 hours of ignition coal gas exposure are more frequent than those in the group less than 5 hours in lead (51.4% vs. 23.9%, p=0.012). Conclusion: Compared to poisoning with other combustible substances, the blood concentration of lead, mercury, and cadmium does not increase further in patients with gas poisoning by ignition coal. However, prolonged exposure may result in elevated levels of lead.

• 한국콘텐츠학회논문지
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• 제21권5호
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• pp.765-775
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• 2021

본 연구의 목적은 정신장애인의 자살생각에 영향을 미치는 요인을 탐색하는 것이다. 이를 위해 독립변수를 개인심리적요인, 사회적요인, 인구사회학적 특성으로 구분하여 분석을 실시하였다. 분석자료는 2017년 보건복지부 연구, '지역사회 정신장애인 현황조사'에 참여한 정신장애인 969명이다. 분석방법은 SPSS 23.0을 이용하여 빈도분석, 기술통계분석, 위계적 다중회귀분석을 실시하였다. 연구결과, 개인심리적 요인에서는 우울이 높을수록, 정신건강자신감이 낮을수록 자살생각이 증가하는 것으로 나타났다. 사회적 요인에서는 미등록장애 집단일수록, 폭력 및 차별 경험에 많이 노출될수록, 대도시에 거주할수록 자살생각이 높아지는 것으로 나타났으며, 이들 변수의 자살생각에 대한 설명력은 26.9%로 나타났다. 이러한 결과를 근거로 도출한 정신장애인의 자살예방에 관한 실천적·정책적 함의는 다음과 같다. 첫째, 정신장애인의 자살생각을 감소시키는 정책과 개입 프로그램을 강구하는데 있어 개인심리적요소에만 초점을 두는 것이 아니라, 사회적요소를 같이 평가하고 고려해야 한다. 둘째, 정신장애인의 자살예방 정책을 강구하는데 있어, 폭력과 차별 경험을 감소시킬 수 있는 권익옹호서비스가 마련되어야 한다. 셋째, 정신장애인의 자살생각을 감소시키기 위해 정신장애등록 제도를 개편하여 정신적 어려움을 경험하고 있는 당사자들이 제도 안으로 포섭될 수 있는 정책적 대안을 강구되어야 한다.

• 농촌의학ㆍ지역보건
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• 제25권2호
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• pp.245-263
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• 2000

본 연구는 자기기입식 설문조사를 통해 농약살포를 대부분 담당하는 남성 농민들에 대하여, 농부증을 포함한 만성증상과 농약살포시의 이상증상경험, 이상증상 경험시 살포한 농약, 농약살포시의 안전수칙 준수, 보호구의 착용실태, 가족 중 농약으로 인한 사고경험을 조사하고, 농약노출과 연관된 변수들간의 연관성, 급성중독경험 및 만성증상과 관련된 요인을 고찰하였다. 만성증상으로는 '몸이 나른하고 힘이 없다', '허리가 아프다'. '밤중에 소변을 보기 위해 깬다' 등의 증상이 많았으며, 농약살포 시의 임상증상 경험율은 '피부발진/가려움증', '어지러움/두통'의 경우가 가장 높은 것으로 조사되었다. 또한, 조사 대상자의 7.1%가 가족중 농약으로 인한 사고경험이 있는 것으로 조사되었으며, 이중 농약살포시 중독, 안전사고 및 자살사례를 확인할 수 있었다. 농약살포 시의 준수사항 중, '뿌린 후 목욕을 한다'와 '뿌린 후 바로 옷을 갈아입는다' 등의 경우가 준수율이 높았으나, '보호구를 잘 세탁해서 보관한다', '뿌린 후 용변을 볼 경우 손을 씻는다', '한낮에는 뿌리지 않는다' 등의 경우는 낮은 준수율을 보였다. 농약살포시 보호구는 '물에 타서 살포할 경우', '입제를 살포할 경우'에 비해 보호구를 더 잘 착용하였으며, 착용율은 모자, 장화, 마스크, 장갑, 방제복 하의의 순이었다. 농약살포시 이상증상경험과 관련된 요인은 농약살포시 준수사항 준수, 살포시간, 과수재배, 경작면적, 연간 농약살포일 등이었으며, 농민의 만성증상과 관련된 관련된 요인은 농약살포시 이상증상 경험, 경작면적, 농사경력, 농약살포시 준수사항 준수, 전업여부, 농약에 대한 노출 등이었다. 본 연구결과를 통해 농약살포시 준수사항의 준수율과 보호구 착용율을 높이며 농약으로 인한 안전사고를 방지하기 위한 체제적인 교육 및 홍보가 필요함을 알 수 있다. 또한, 농약에 대한 노출이 농민의 만성증상의 한 원인이라는 결론을 내리기는 어려우나, 원인적 연관성일 가능성을 배재할 수는 없을 것으로 생각되며, 따라서, 농약노출을 정확히 산출하고, 좀더 명확한 건강영향의 생물학적 지표(biological marker)를 측정하여 이들의 연관성을 고찰하는 연구가 이루어져야 할 것으로 생각된다.

• 대한임상독성학회지
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• 제5권1호
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• pp.36-42
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• 2007

Purpose: To assess the epidemiology and the clinical features of Glufosinate herbicide in Korea. Methods: Data was prospectively collected during 1 year since August 2005 from 38 hospital in Korea. We analyzed the epidemiologic characters and clinical manifestations of Glufosinate poisoning. In addition, the characteristics of patients with severe central nervous system toxicity were separately analyzed to find poor prognosis relating factors. Results: During study periods, there were 715 persons of poisoning of herbicides and insecticides. 6.3% (45 persons) of the agricultural chemicals poisoning had Glufosinate poisoning. There were 36 cases of suicide attempts and 7 cases of accidental exposure, The major of poisoning route was oral ingestion (44 cases). 28.9 % of the study patients had not toxic symptoms. The most common symptoms were gastrointestinal symptoms relating surfactant irritation. 67.7% of central nervous symptoms occurred lately. 10 persons showed severe central nervous system toxicity. 4 persons of them showed poor outcomes (1 death, 3 hopeless discharged). Complications of respiratory failure and renal failure related with poor outcome. Conclusion: Majority of patients ingested Glufosinate for suicide attempt. 22.2% of patients with Glufosinate poisoning showed delayed serious central nervous system toxicity. Early supportive care of altered mentality may prevent late respiratory complications and improve the outcomes.

• 대한임상독성학회지
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• 제10권2호
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• pp.73-79
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• 2012

Purpose: The purpose of this study was to identify the changes in the characteristics of patients with carbon monoxide (CO) poisoning, as well as the distinctive differences in intentionally exposed patients. Methods: The medical records of CO poisoning patients, who visited nine emergency departments between January 2010 and December 2011, were reviewed retrospectively. The clinical information including age, gender, hospitalization, type of discharge, cause and location of exposure, site of onset, concentration of initial blood carboxyhemoglobin (COHb), methods of treatment and presence of neurological complications was examined. The subjects were divided into an intentional and non-intentional group and the differences between them was compared. Results: A total 209 subjects were recruited. The median age was 38 years (29~49.5 years). They frequently complained of nausea and vomiting, and the most common exposures occurred in winter, normally in the home. The cause of exposure was usually fire, followed by incomplete combustion of fuels. The median initial blood COHb was 13.15%. The proportion of intentionally exposed patients was 21%. They were significantly younger, more frequently discharged against medical advice, and showed a higher initial blood COHb level (22.85%) than the non-intentional group. Conclusion: This study suggests that those with intentional CO poisoning are normally discharged against medical advice even when they have a higher initial COHb level. An adequate explanation of the delayed neurologic sequelae and short term follow-up observation is recommended for those patients with intentional exposure.

• 대한임상독성학회지
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• 제16권2호
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• pp.157-160
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• 2018

Chronic silica nephropathy has been associated with tubulointerstitial disease, immune-mediated multisystem disease, chronic kidney disease, and end-stage renal disease. On the other hand, acute intentional exposure is extremely rare. The authors' experienced a 44-year-old man who took rapid cement hardener (sodium silicate) in a suicide attempt whilst in a drunken state. He visited the emergency department approximately 1 hour after ingestion. Information on the material was obtained after 3 L gastric lavage. The patient complained of a sore throat, epigastric pain, and swollen to blood tinged vomitus. Proton pump inhibitors, hemostats, steroid, and fluids were administered. Nine hours after ingestion, he was administered 200 mL hematochezia. Immediately after, a gas-troenterologist performed an endoscopic procedure that revealed diffuse hyperemic mucosa with a color change and variable sized ulceration in the esophagus, whole stomach, and duodenal $2^{nd}$ portion. Approximately 35 hours later, persistent oligouria and progressive worsening of the renal function parameters (BUN/Cr from 12.2/1.2 to 67.5/6.6 mg/dL) occurred requiring hemodialysis. The patient underwent 8 sessions of hemodialysis for 1 month and the BUN/Cr level increased to 143.2/11.2 mg/dL and decreased to 7.6/1.5 mg/dL. He was discharged safely from the hospital. Follow up endoscopy revealed a severe esophageal stricture and he underwent endoscopic bougie dilatation. Acute cement hardener (sodium silicate) intoxication can cause renal failure and strong caustic mucosal injury. Therefore, it is important to consider early hemodialysis and treatment to prevent gastrointestinal injury and remote esophageal stricture.

• 대한임상독성학회지
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• 제12권1호
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• pp.8-13
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• 2014

Purpose: Glyphosate (N-phosphonomethyl glycine) is widely used as an herbicide for weed control in rural areas. It is also readily available for suicide attempts. Glyphosate has high toxicity and negatively affects the human body. The aim of this investigation was to study the genotoxicity of a low-concentration of glyphosate through sister chromatid exchange (SCE) in human blood lymphocytes in vitro. Methods: Primary lymphocyte cultures were obtained from blood samples of 11 males and seven females who had been exposed to glyphosate (0, 100, 200, and 300 ng/mL). The frequency of SCEs was examined and statistical analysis was performed. Results: All doses of glyphosate induced a significant dose-dependent increase in SCE frequency compared with the control group (P<0.001). In particular, the SCE frequency for exposure to low-dose glyphosate was significantly higher in females than in males. Conclusion: According to the result of this study, even a low-dose of glyphosate may damage DNA and females are more vulnerable to glyphosate.

• Journal of Microbiology and Biotechnology
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• 제22권6호
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• pp.856-865
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• 2012

We describe the construction and immunobiological properties of a novel whooping cough vaccine candidate, in which the aroQ gene, encoding 3-dehydroquinase, was deleted by insertional inactivation using the kanamycin resistance gene cassette and allelic exchange using a Bordetella suicide vector. The aroQ B. pertussis mutant required supplementation of media to grow but failed to grow on an unsupplemented medium. The aroQ B. pertussis mutant was undetectable in the trachea and lungs of mice at days 6 and 12 post-infection, respectively. Antigen-specific antibody isotypes IgG1 and IgG2a, were produced, and cell-mediated immunity [CMI], using interleukin-2 and interferon-gamma as indirect indicators, was induced in mice vaccinated with the aroQ B. pertussis vaccine candidate, which were substantially enhanced upon second exposure to virulent B. pertussis. Interleukin-12 was also produced in the aroQ B. pertussis-vaccinated mice. On the other hand, neither IgG2a nor CMI-indicator cytokines were produced in DTaP-vaccinated mice, although the CMI-indicator cytokines became detectable post-challenge with virulent B. pertussis. Intranasal immunization with one dose of the aroQ B. pertussis mutant protected vaccinated mice against an intranasal challenge infection, with no pathogen being detected in the lungs of immunized mice by day 7 post-challenge. B. pertussis aroQ thus constitutes a safe, non-reverting, metabolite-deficient vaccine candidate that induces both humoral and cell-mediated immune responses with potential for use as a single-dose vaccine in adolescents and adults, in the first instance, with a view to disrupting the transmission cycle of whooping cough to infants and the community.