• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.150-150
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• 2003

• Korean Journal of Plant Resources
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• v.7 no.2
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• pp.115-119
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• 1994

These experiments were conducted to determine the effects of Sorghum allelopathic substances on the callus growh of several weeds and crops. 1. When substances extracted from allelopathic Sorghum(Sorghum bicolor L.) were treated on medium, growth of callus of several weeds and crops were in-hibited. The degree of inhibition differed depending on the genotypes, ranging from 50 to 90% com-pared with that of control. 2. The extracts of above 5% Sorghum inhibited the callus growth of Che-nopodium albun L., Commelina communis L., and .Ammaranthus retroflexus L.and showed in-hibition rate of above 70% in callus growth. These results indicate that we could investigate theallelopaihy effect by using in vitro system. 3. The suitable explant for callus induction fromallelopathic plants was immature embryos, the callus induction rate differed depending on the geno-type, growth regulators and concentrations. In general, the addition of 2, 4-D and NAA onto medium increased the rate and amount of callus.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.26 no.6
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• pp.602-605
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• 2000

Human alveolar bone cells were isolated from alveolar bone fragments obtained from normal individual undergoing third molar extractions. Alveolar bone fragments were cultured as explant. Cells began to migrate in the first $5{\sim}7$ day and were confluent in $5{\sim}7$ week. Matrix mineralization was observed by 4 week. Our studies utilize established protocols for the characterization of these cells as osteoblasts by means of alkaline phosphatase activity determination, identification of osteocalcin antigens, establishing the presence of cells expressing type I collagen and determining the ability of cells to produce calcification. Transmission electron microscopic observations confirmed the presence of a collagen matrix undergoing a mineralization process. This new model, using human alveolar bone cells, may provide a tool to investigate alveolar bone development and physiology and to set up new therapeutic approaches.

• Plant Resources
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• v.1 no.2
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• pp.109-112
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• 1998

Aloe in vitro culture was attempted to induce callus and regeneration ability from different explant sources onto MS medium with 0.5mg/l NAA plus 1.0mg/l BA. Anthers that no developed any callus and plant regeneration, while only four out of 274 filament explants induced calli at cut edge without regenerated plants. Twenty ovary explants regenerated four direct plantlets without via callus from the base of epidermal tissues. Regenerated plants on the root tip gave 2n=14 of chromosome numbers.

• Journal of Plant Biotechnology
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• v.30 no.3
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• pp.241-244
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• 2003

Root cluster section culture, showing high efficient Protocorm-like body (PLB) formation capacity, were established in Doritaenopsis hybrids. Three types of root were obtained from excised shoots in 1/2MS medium containing different concentrations of NAA; \circled1normal roots, \circled2multiple roots and \circled3abnormal root clusters. Those were placed on 1/2MS medium supplemented with 0.5 mg/L thidiazuron for PLB regeneration. PLB regeneration rate was greater in root cluster section cultures (77.8%) compare to normal root tip cultures(30%). Number of PLBs regenerated from root cluster sections were counted over 11 per explant (5.3 per normal root tip).High frequency of PLB regeneration was achieved in root cluster section culture. This result can be used as an efficient method for clonal proliferation of Doritaenopsis hybrids.

• Journal of Plant Biotechnology
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• v.7 no.3
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• pp.169-174
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• 2005

The optimal culture conditions were studied for plant regeneration from mesophyll protoplasts of Solanum sisymbriifolium. Axenic seedlings of S. sisymbriifolium were used as a explant for protoplast culture. Many viable protoplasts were isolated by incubating leaf slices in an enzyme solution containing 0.25% Meicerase and 0.05% Macerozyme for 16 hr at $25^{\circ}C$ without shaking. Protoplast density of $5.0{\times}10^4\;ml^{-1}$ in Kao medium containing 5.0 mg/L NAA, 1.0 mg/L 2,4-D and 1.0 mg/L BA was optimal for colony formation. Most colonies were formed when protoplasts were cultured at $25^{\circ}C$ after initial culture at $30^{\circ}C$ for one week. On the MS agar medium with 1.0 mg/L zeatin, 38.4% of protoplast-derived calli differentiated shoots. These shoots rooted on 1/2MS medium with 5.0 g/L sucrose and 2.5 g/L gellan gum, and developed into whole plants.

• Journal of Plant Biotechnology
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• v.6 no.3
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• pp.181-188
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• 2004

Zingiber officinale buds from the rhizomes were used to produce in vitro shoots. These explants produced the largest number of multiple shoots, 9.8 shoots per explant, when were cultured on MS (Murashige and Skoog 1962) medium supplemented with 2.0 mg/L benzyladenine (BA) and 2.0 mg/L indole butyric acid (IBA). This medium was also found to be suitable for in vitro propagation of other Zingiberaceae species: Alpinia conchigera, Alpinia galanga, Curcuma domestica, C. zedoaria and Kaempferia galanga. Both C. domestica and C. zedoaria produced more multiple shoots when were cultured in the liquid proliferation medium, MS medium containing 2.0 mg/L BA and 2.0 mg/L IBA. To maintain the in vitro plantlets of Zingiberaceae species, they were required to subculture every four weeks. After executing proper acclimatization protocol, in vitro plantlets of Alpinia galanga, A. conchigera, Curcuma domestica, C. zedoaria, Kaempferia galanga and Zingiber officinale could be successfully planted in the field with high percentage of survival.

• Korean Journal of Medicinal Crop Science
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• v.11 no.4
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• pp.316-320
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• 2003

A protocol is described for rapid multiplication of Zanthoxylum piperitum DC. (Rutaceae), an important aromatic and medicinal plant, through shoot-tip explant cultures. Murashige and Skoog (MS) medium supplemented with various concentrations of N-6-benzyladenine (BA), N-6-benzylaminopurine (BAP) and thidiazuron (TDZ), in single or in combination with ${\alpha}-naphthaleneacetic$ acid (NAA), was used to determine the rate of shoot proliferation. N-6-benzyladenine (BA) used at 0.5mg/l, was the most effective in initiating multiple shoot proliferation at the rate of 23 microshoots per shoot-tip explants after 40 days of culture. Shoot multiplication increased 1.2-fold in each successive subculture. Induction of rooting (98%) was achieved by transferring the shoots to the same basal medium containing 2 mg/l indole-3-butyric acid (IBA). Plantlets went through a hardening phase in a controlled growth chamber, prior to in vivo transfer. These results represented that possible application for the mass production of plantlets through in vitro culture system of Zanthoxylum piperitum DC.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.3
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• pp.279-283
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• 2011

The immature embryos during seed development were examined to predict the suitable embryos for an efficient regeneration system. Five spring wheat genotypes and five winter wheat genotypes were tested using immature embryos as explants. Spring wheat genotypes showed much higher levels of plant regeneration than those of winter wheat genotypes. The highest frequencies of embryogenesis and regeneration were obtained when embryos at 13-14 days after anthesis (DAA) were used as explant and decreased using embryos at 21-22 DAA during seed development. Significant differences were also found for callus induction and regeneration as affected by immature embryo size. The regeneration efficiency was drastically decreased in spring and winter wheat genotypes when embryos larger than 2.0 mm of length were used. The optimum developmental stage and embryo length for regeneration efficiency were at 13-14 DAA and 1.0-1.5 mm, respectively. The selection of suitable embryos for the high frequencies of embryogenesis and regeneration leads us to efficient genetic improvement of wheat.

• Journal of Ginseng Research
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• v.5 no.1
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• pp.35-40
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• 1981

Explants of mature root tissues and calli derived from root and cotyledon of Panax ginseng were cultured in vitro on Murashige and Skoog medium supplemented with 2, 4-dichlorophen-oxyacetic acid(3,4-D), naphthaleneacetic acid(NAA), benzyladenine, and gibberellic acid to assess their capacity to regenerate organs. Root formation at high percentage (46.2-61.1%) was obtained 20-30 days after culturing on media supplemented with combinations of NAA(5 mg/l) and kinetin (1 mg/l), And calli derived from cotyledon produced numerous embryoids in media($\frac{1}{2}$MS) containing 2,4-D(0.5 mg/l) and kinetin (0.5 mg/l). Reculture of these embryoids in media($\frac{1}{2}$MS) enriched with 1 mg/l of benzyladenine and 1 mg/l of gibberellic acid resulted in more plantlet regeneration.