• BMB Reports
• /
• 제41권10호
• /
• pp.739-744
• /
• 2008

Turnip yellow mosaic virus (TYMV) is a positive strand RNA virus that infects mainly Cruciferae plants. In this study, the TYMV genome was modified by inserting an extra subgenomic RNA promoter and a multiple cloning site. This modified TYMV was introduced into Nicotiana benthamiana using a Agrobacterium-mediated T-DNA transfer system (agroinfiltration). When a gene encoding $\beta$-glucuronidase or green fluorescent protein was expressed using this modified TYMV as a vector, replication of the recombinant viruses, especially the virus containing $\beta$-glucuronidase gene, was severely inhibited. The suppression of replication was reduced by co-expression of viral silencing suppressor genes, such as tombusviral p19, closteroviral p21 or potyviral HC-Pro. As expected, two subgenomic RNAs were produced from the recombinant TYMV, where the larger one contained the foreign gene. An RNase protection assay revealed that the recombinant subgenomic RNA was encapsidated as efficiently as the genuine subgenomic RNA.

• 한국건축시공학회:학술대회논문집
• /
• 한국건축시공학회 2017년도 춘계 학술논문 발표대회
• /
• pp.272-273
• /
• 2017

As businesses put a greater emphasis on outward appearances, the demand for external window cleaning has been on the rise. However, with the conventional labor-intensive window cleaning method, it is hard to meet this demand. Therefore, this study proposes a Life Cycle Cost (LCC) analysis model for a guiderail-type cleaning robot, and estimate the LCCs of the conventional method and the cleaning robot. The findings of this research are expected to serve as a guide for future development of a cleaning robot.

• 한국결정성장학회지
• /
• 제25권1호
• /
• pp.13-19
• /
• 2015

Among the various possible factors affecting the Minority Carrier Life Time (MCLT) of the mc-Si crystal, dislocations formed during the cooling period after solidification were found to be a major element. It was confirmed that other defects such as grain boundary or twin boundary were not determinative defects affecting the MCLT because most of these defects seemed to be formed during the solidification period. With a measurement of total thickness variation (TTV) and bow of the silicon wafers, it was found that residual stress remaining in the mc-Si crystal might be another major factor affecting the MCLT. Thus, it is expected that better quality of mc-Si can be grown when the cooling process right after solidification is carried out as slow as possible.

• 한국철도학회:학술대회논문집
• /
• 한국철도학회 2002년도 추계학술대회 논문집(I)
• /
• pp.396-407
• /
• 2002

신규로 투입되는 전동차는 강화된 안전성과 승객 편의성 규격이 요구되며 이로 인해 차량 초기 구입 비용 증가가 불가피할 것으로 예상되나 효율적인 설계를 통한 운영비 절감 등 또한 예상된다. 따라서 차량 구입은 초기 구입 비용, 운영 비용을 고려하여 결정되어야 하며, 이러한 비용 추정의 한 방법으로써 차량 수명 주기 비용(Life Cycle)과 그 적용 가능성을 제시하고자 한다.

• 산업공학
• /
• 제14권2호
• /
• pp.205-209
• /
• 2001

The number of tunnel has fast increased with the rapid expansion of highway network. Tunnel should be designed to provide for drivers both safety and pleasant driving conditions. In this perspective, the design for tunnel lightning is very important in order to provide its safety, pleasantness, and cost-efficienty of maintenance, all of which should be considered and analyzed for a better tunnel lighting. This paper attempts to compare the low-pressure sodium lamp, which has usually been used for tunnel lighting, with the fluorescent lamp, which we consider as an alternative for the former. In an effort to determine the number of lamps to meet the required illuminance in the tunnel, this research employs a simulation technique which would allow us to conjecture, with the aid of basic model, the life cycle cost for illumination per each tunnel. This analysis is expected to provide a basic method and related information for tunnel development and design.

• Industrial Engineering and Management Systems
• /
• 제3권1호
• /
• pp.59-62
• /
• 2004

The number of tunnel has fast increased with the rapid expansion of highway network. Tunnel should be designed to provide for drivers both safety and pleasant driving conditions. In this perspective, the design for tunnel lightning is very important in order to provide its safety, pleasantness, and cost-efficiency of maintenance, all of which should be considered and analyzed for a better tunnel lighting. This paper attempts to compare the low-pressure sodium lamp, which have usually been used for tunnel lighting, with the fluorescent lamp, which we consider as an alternative for the former. In an effort to determine the number of lamps to meet the required illuminance in the tunnel, this research employs a simulation technique which would allow us to conjecture, with the aid of basic model, the life cycle cost for illumination per each tunnel. This analysis is expected to provide a basic method and related information for tunnel development and design.

• 한국정밀공학회지
• /
• 제21권5호
• /
• pp.158-165
• /
• 2004

Up to now, many crack repair techniques have been developed for inhibiting crack growth in structural components. However, the simplest way for inhibiting crack growth is to apply a indentation at the crack tip or at some distance ahead of the expected crack growth path so as to produce residual compressive stresses that can reduce the effective stresses around the crack tip. In spite of its importance to the aerospace industry, little attention has been devoted to evaluation of the indentation residual stress effect on the fatigue crack initiation life quantitatively. Therefore, in the present work, the magnitude and distribution of the indentation residual stresses were investigated in order to estimate the beneficial effect on fatigue crack initiation by using finite element method. Furthermore, to examine the validity of finite element analysis results, residual stress distribution in the indented specimen was measured by using X-ray diffraction technique, and fatigue crack behavior at fastener hole in aluminum alloy 7075-T6 before and after indentation processes was investigated.

• 식물분류학회지
• /
• 제47권3호
• /
• pp.199-206
• /
• 2017

The last four years of joint botanical collections by the governments of Myanmar and South Korea in Natma Taung National Park and adjacent areas in the Chin State of Myanmar have revealed the presence of 20 naturally occurring species of angiosperms new to the flora of Myanmar. Plants not previously recorded include species originally considered to be only found in neighboring mega-diverse countries. Examples (e.g., for India) include Boehmeria manipurensis Friis & Wilmot-Dear (Urticaceae), Trigonotis hookeri Benth. ex C. B. Clarke (Boraginaceae) and Mycetia radiciflora (C. B. Clarke) Airy Shaw (Rubiaceae); those for China include Microtoena delavayi Prain (Lamiaceae), Pimpinella kingdon-wardii H. Wolff (Apiaceae) and Senecio diversipinnus Y. Ling (Asteraceae). The data presented in this report are expected to be useful sources for phytogeographical studies of these species.

• 대한토목학회논문집
• /
• 제33권5호
• /
• pp.2081-2091
• /
• 2013

최근, 엔지니어링의 실무에서 토목 시설물의 설계 및 유지관리 단계를 위한 효과적인 생애주기비용(Life Cycle Cost; LCC) 산정 방법의 실무적인 적용에 대한 요구가 높아지고 있다. 이와 같이, 21세기에 들어 엔지니어링의 최적 의사결정의 실무적 문제에 가치공학과 더불어 생애주기비용 분석은 새로운 패러다임으로 주목받고 있지만 이러한 연구 개발의 괄목할만한 진보에도 불구하고, 대부분의 설계단계 생애주기비용 분석은 확정적, 확률적 분석기법에 그치고 있고 적용 가능한 구조물도 일반 교량에 국한되어 있다. 따라서 본 논문은 설계단계 생애주기비용 분석에 대한 실용적이고 합리적인 신뢰성해석 기반 성능저하 모델을 고려하여 기존의 분석방법을 업데이트하는 분석 방법론을 개발하고 이를 특수교인 사장교에 적용하는데 목적이 있다. 이에 현재가치의 합으로 표현되는 직/간접 유지관리비용을 기존 방법과는 다르게 기대 성능저하모델에 바탕을 둔 최적 유지관리 시나리오를 통한 생애주기비용 분석 기법으로 제시하였다. 마지막으로 본 논문에서 제안한 업데이트된 생애주기비용 분석의 방법론을 실제 고속도로 사장교 건설 프로젝트의 설계단계 의사결정 문제에 적용하여 합리적이고 체계적인 분석 방법 및 최적의사결정과정을 제시하였다.

• 한국강구조학회 논문집
• /
• 제17권2호통권75호
• /
• pp.227-241
• /
• 2005

본 논문에서는 생애주기동안 발생하는 부식이나 일평균교통량 및 중차량의 통행량와 같은 사용환경에 의존하는 강교의 생애신뢰성에 기초한 생애주기비용(Life-Cycle Cost: 이하 LCC) 최적설계 방법론을 제안하였다. 강교 최적설계를 위한 LCC는 초기비용, 생애주기 기대유지관리비용, 생애주기 기대직접복구비용과 인적 혹은 물적손실비용, 도로이용자비용, 그리고 사회-경제 손실비용을 포함하는 간접복구비용을 현재가치의 합으로 정식화하였다. 이러한 LCC비용항목 중에서 생애주기 복구비용의 산정을 위해서는 하중과 저항이력에 의존하는 누적손확률로부터 산정되는 연파손확률이 고려되어야한다. 이를 위해 본 논문에서는 Nowak의 활하중 모델(1993)과 부식개시, 부식률, 그리고 재도장영향을 고려한 수정된 부식모델을 제안하였다. 이와같이 본 연구에서 제안된 LCC 최적설계 방법론은 3 경간연속(40m+50m+40m= 130m)의 실제 강박스거더교에 적용되었고, 사용환경에 대한 LCC의 효율성에 대해 비교 고찰하였다. 적용예를 통해 부식환경, 일평균교통량, 그리고 중차량의 통행량는 강교 LCC최적설계에 매우 중요한 영향을 미칠 수 있음을 알 수 있었으며, 이러한 교량의 사용환경인자들은 경우에 따라 LCC 최적설계에 대한 주요 변수로 고려되어야 할 것으로 판단된다.