• BMB Reports
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• v.41 no.10
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• pp.739-744
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• 2008

Turnip yellow mosaic virus (TYMV) is a positive strand RNA virus that infects mainly Cruciferae plants. In this study, the TYMV genome was modified by inserting an extra subgenomic RNA promoter and a multiple cloning site. This modified TYMV was introduced into Nicotiana benthamiana using a Agrobacterium-mediated T-DNA transfer system (agroinfiltration). When a gene encoding $\beta$-glucuronidase or green fluorescent protein was expressed using this modified TYMV as a vector, replication of the recombinant viruses, especially the virus containing $\beta$-glucuronidase gene, was severely inhibited. The suppression of replication was reduced by co-expression of viral silencing suppressor genes, such as tombusviral p19, closteroviral p21 or potyviral HC-Pro. As expected, two subgenomic RNAs were produced from the recombinant TYMV, where the larger one contained the foreign gene. An RNase protection assay revealed that the recombinant subgenomic RNA was encapsidated as efficiently as the genuine subgenomic RNA.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2017.05a
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• pp.272-273
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• 2017

As businesses put a greater emphasis on outward appearances, the demand for external window cleaning has been on the rise. However, with the conventional labor-intensive window cleaning method, it is hard to meet this demand. Therefore, this study proposes a Life Cycle Cost (LCC) analysis model for a guiderail-type cleaning robot, and estimate the LCCs of the conventional method and the cleaning robot. The findings of this research are expected to serve as a guide for future development of a cleaning robot.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.25 no.1
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• pp.13-19
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• 2015

Among the various possible factors affecting the Minority Carrier Life Time (MCLT) of the mc-Si crystal, dislocations formed during the cooling period after solidification were found to be a major element. It was confirmed that other defects such as grain boundary or twin boundary were not determinative defects affecting the MCLT because most of these defects seemed to be formed during the solidification period. With a measurement of total thickness variation (TTV) and bow of the silicon wafers, it was found that residual stress remaining in the mc-Si crystal might be another major factor affecting the MCLT. Thus, it is expected that better quality of mc-Si can be grown when the cooling process right after solidification is carried out as slow as possible.

• Proceedings of the KSR Conference
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• 2002.10a
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• pp.396-407
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• 2002

New Electric Multiple Units that will be released in the rolling stock market are required to meet regulations of reinforced safety and conveniences for passengers. For those reasons, Vehicle purchasing cost to be increased will be unavoidable. However, The reduction of operating cost by employing effective and efficient design on the EMU at initial phase will be expected. Hence, Vehicle purchasing shall be determined to take into consideration of purchasing cost vehicle at first stage and operation cost appropriately. In order for introducing method to presume such costs, this document will show Life Cycle Cost on vehicle and its applicability.

• IE interfaces
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• v.14 no.2
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• pp.205-209
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• 2001

The number of tunnel has fast increased with the rapid expansion of highway network. Tunnel should be designed to provide for drivers both safety and pleasant driving conditions. In this perspective, the design for tunnel lightning is very important in order to provide its safety, pleasantness, and cost-efficienty of maintenance, all of which should be considered and analyzed for a better tunnel lighting. This paper attempts to compare the low-pressure sodium lamp, which has usually been used for tunnel lighting, with the fluorescent lamp, which we consider as an alternative for the former. In an effort to determine the number of lamps to meet the required illuminance in the tunnel, this research employs a simulation technique which would allow us to conjecture, with the aid of basic model, the life cycle cost for illumination per each tunnel. This analysis is expected to provide a basic method and related information for tunnel development and design.

• Industrial Engineering and Management Systems
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• v.3 no.1
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• pp.59-62
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• 2004

The number of tunnel has fast increased with the rapid expansion of highway network. Tunnel should be designed to provide for drivers both safety and pleasant driving conditions. In this perspective, the design for tunnel lightning is very important in order to provide its safety, pleasantness, and cost-efficiency of maintenance, all of which should be considered and analyzed for a better tunnel lighting. This paper attempts to compare the low-pressure sodium lamp, which have usually been used for tunnel lighting, with the fluorescent lamp, which we consider as an alternative for the former. In an effort to determine the number of lamps to meet the required illuminance in the tunnel, this research employs a simulation technique which would allow us to conjecture, with the aid of basic model, the life cycle cost for illumination per each tunnel. This analysis is expected to provide a basic method and related information for tunnel development and design.

• Journal of the Korean Society for Precision Engineering
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• v.21 no.5
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• pp.158-165
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• 2004

Up to now, many crack repair techniques have been developed for inhibiting crack growth in structural components. However, the simplest way for inhibiting crack growth is to apply a indentation at the crack tip or at some distance ahead of the expected crack growth path so as to produce residual compressive stresses that can reduce the effective stresses around the crack tip. In spite of its importance to the aerospace industry, little attention has been devoted to evaluation of the indentation residual stress effect on the fatigue crack initiation life quantitatively. Therefore, in the present work, the magnitude and distribution of the indentation residual stresses were investigated in order to estimate the beneficial effect on fatigue crack initiation by using finite element method. Furthermore, to examine the validity of finite element analysis results, residual stress distribution in the indented specimen was measured by using X-ray diffraction technique, and fatigue crack behavior at fastener hole in aluminum alloy 7075-T6 before and after indentation processes was investigated.

• Korean Journal of Plant Taxonomy
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• v.47 no.3
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• pp.199-206
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• 2017

The last four years of joint botanical collections by the governments of Myanmar and South Korea in Natma Taung National Park and adjacent areas in the Chin State of Myanmar have revealed the presence of 20 naturally occurring species of angiosperms new to the flora of Myanmar. Plants not previously recorded include species originally considered to be only found in neighboring mega-diverse countries. Examples (e.g., for India) include Boehmeria manipurensis Friis & Wilmot-Dear (Urticaceae), Trigonotis hookeri Benth. ex C. B. Clarke (Boraginaceae) and Mycetia radiciflora (C. B. Clarke) Airy Shaw (Rubiaceae); those for China include Microtoena delavayi Prain (Lamiaceae), Pimpinella kingdon-wardii H. Wolff (Apiaceae) and Senecio diversipinnus Y. Ling (Asteraceae). The data presented in this report are expected to be useful sources for phytogeographical studies of these species.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.33 no.5
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• pp.2081-2091
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• 2013

Recently, the demand on the practical application of life-cycle cost effectiveness for design and rehabilitation of civil infrastructure is rapidly growing unprecedently in civil engineering practice. Accordingly, in the 21st century, it is almost obvious that life-cycle cost together with value engineering will become a new paradigm for all engineering decision problems in practice. However, in spite of impressive progress in the researches on the LCC, the most researches have only focused on the Deterministic or Probabilistic LCC analysis approach and general bridge at design stage. Thus, the goal of this study is to develop a practical and realistic methodology for the Life-Cycle Cost LCC-effective optimum decision-making based on reliability analysis of bridges at design stage. The proposed updated methodology is based on the concept of Life Cycle Performance(LCP) which is expressed as the sum of present value of expected direct/indirect maintenance costs with expected optimal maintenance scenario. The updated LCC methodology proposed in this study is applied to the optimum design problem of an actual highway bridge with Cable Stayed Bridges. In conclusion, based on the application of the proposed methods to an actual example bridge, it is demonstrated that a updated methodology for performance-based LCC analysis proposed in this thesis, shown applicably in practice as a efficient, practical, process LCC analysis method at design stage.

• Journal of Korean Society of Steel Construction
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• v.17 no.2 s.75
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• pp.227-241
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• 2005

This paper presents a practical and realistic Life-Cycle Cost (LCC) optimum design methodology for steel bridges considering the long-term effect of environmental stressors such as corrosion and heavy truck traffics on bridge reliability. The LCC functions considered in the LCC optimization consist of initial cost, expected life-cycle maintenance cost, and expected life-cycle rehabilitation costs including repair/replacement costs, loss of contents or fatality and injury losses, road user costs, and indirect socio-economic losses. For the assessment of the life-cycle rehabilitation costs, the annual probability of failure, which depends upon the prior and updated load and resistance histories, should be accounted for. For the purpose, Nowak live load model and a modified corrosion propagation model, which takes into consideration corrosion initiation, corrosion rate, and repainting effect, are adopted in this study. The proposed methodology is applied to the LCC optimum design problem of an actual steel box girder bridge with 3 continuous spans (40m+50m+40m=130m). Various sensitivity analyses are performed to investigate the effects of various design parameters and conditions on the LCC-effectiveness. From the numerical investigation, it has been observed that local corrosion environments and the volume of truck traffic significantly influence the LCC-effective optimum design of steel bridges. Thus, these conditions should be considered as crucial parameters for the optimum LCC-effective design.