• Asian Pacific Journal of Cancer Prevention
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• 제15권16호
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• pp.6773-6779
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• 2014

Background: Nanotechnology opens new applications in many fields including medicine. Among all metallic nanoparticles, silver nanoparticles (silver NPS) have proved to be the most effective against a large variety of organisms including toxic cyanobacteria. Materials and Methods: Silver NPs were biosynthesized in vivo with different alga species namely, Spirulina piatensis, Chlorella vulgaris and Scenedesmus oh/iquus following two scenarios. First: by suspending a thoroughly washed algae biomass in 1 mM aqueous $AgN0_3$ solution. Second: by culturing them individually in culture media containing the same concentration of $AgN0_3$. Silver NPs were characterized using UV-Vis spectroscopy, transmission electron microscopy (TEM), energy dispersive analysis (EDX) and Fourier transform infra-red (FfIR) spectroscopy. The biosynthesized silver NPs were tested for cytotoxic activity against a cancer promoter cyanobacteruim Microcystis aeruginosa, considering effects on cell viability and chlorophyll content. Results: The surface plasmon band indicated the biosynthesis of silver NPs at ~400 nm. Transmission electron microscopy (TEM) revealed that the silver NPs had a mean average size below 100 nm. Energy-dispersive analysis X-ray (EDX) spectra confirmed the presence of silver element. FfIR spectral analyses suggested that proteins and or polysaccharides may be responsible for the biosynthesis of silver NPs and (-COO-) of carboxylate ions is responsible for stabilizing them. The toxic potentialities ofthe biosynthesized silver NPs against the cancer promoter cyanobacterium, Microcystis aeruginosa showed high reduction in viable cells count and the total chlorophyll content. Conclusions: The potential activity of the biosynthesized silver NPs from the studied algae species against Microcystis aernginosa cells is expected to be mainly mediated by the release of silver ions (Ag+) from the particle surface and bioactive compounds as indicated by FfIR analysis.

• 한국연초학회지
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• 제21권1호
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• pp.82-88
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• 1999

The present study was conducted to compare the relative efficiency of two different haploid breeding methods in tobacco varietal development. A single FI hybrid plant from cross of two flue-cured cultivars of Nicotiana tabacum L., Bright Yellow4(BY4) and NC95, was used to develop the 30 anther derived dihaploid (ADH) lines and maternally-derived dihaploid (MDH) lines utilizing Nicotiana africana, respectively. As compared with mid-parent, mean of ADH lines showed increase in number of leaves, delay in days to flower and narrow in width of leaf. However, no significant differences in the other investigated characters were recognized. MDH lines also showed narrow width of leaf, while no significant differences in the other characters were observed. The variations of the investigated characters were generally greater in ADH than MDH lines. MDH lines had higher height of plant and shorter days to flower than ADH lines, while the other characters did not show remarkable differences. The degree of heritability for each of the characters observed between ADH and MDH was almost the same. The characters showing high heritability value were height of plant, number of leaves, days to flower, and yield, while those showing relatively low value were length of leaf, width of leaf, and total alkaloid content. Predicted gains from selection for increased yield were calculated for both populations(ADH, MDH) and correlated responses associated with selection for yield were estimated. Height of plant, width of leaf, days to flower, and concentration of reduceing sugar would be expected to improve with selection for yield much faster in the MDH population than in the ADH.

• Archives of Pharmacal Research
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• 제26권11호
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• pp.892-897
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• 2003

Anticancer drugs have serious side effects arising from their poor malignant cells selectivity, Since insulin receptors highly express on the cytomembrane of some kind of tumor cells, using insulin as the vector was expected to reduce serious side effects of the drugs. The objective of this study was to evaluate the tumor targeting effect of the newly synthesized mitoxantrone-insulin conjugate (MIT-INS) with the drug loading of 11.68%. In vitro stability trials showed MIT-INS were stable in buffers with different pH (2-8) at $37^{\circ}C$ within 120 h (less than 3% of free MIT released), and were also stable in mouse plasma within 48 h (less than 1 % of free MIT released). In vivo study on tumor-bearing mice showed that, compared with MIT [75.92 $\mu g \cdot$ h/g of the area under the concentration-time curve (AUC) and 86.85 h of mean residence time (MRT)], the conjugates had better tumor-targeting efficiency with enhanced tumor AUC of 126.53 1l9 h/g and MTR of 151.95 h. The conjugate had much lower toxicity to most other tissues with targeting indexes ($TI^c$) no larger than 0.3 besides good tumor targeting efficiency with $TI^c$ of 1.67. The results suggest the feasibility to promote the curative effect in ca.ncer chemotherapy by using insulin as the vector of anti-cancer drugs.

• The Korean Journal of Ecology
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• 제23권6호
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• pp.423-429
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• 2000

To understand the effects of elevated $CO_2$ concentration and temperature on seedling emergence of seven herbaceous species, the seedling emergence was monitored between November 1997 and May 1998 using a temperature gradient chamber and a $CO_2$-temperature gradient chamber. Experiment was conducted under current ambient condition (Control plot), 2$^{\circ}C$-warmed condition with ambient $CO_2$ (T2 Plot), 4$^{\circ}C$-warmed condition with ambient $CO_2$ (T4 plot). and 4$^{\circ}C$-warmed condition with 1.8 fold of ambient $CO_2$ (CT4 plot). Species tested in this study were Digitaria adscendens, Echinochloa crus-galli, Panicum bisulcatum, Setaria viridis. Oenothera biennis, Andropogon virginicus, and Imperata cylindrica. Each species often dominates in the herbaceous stage of secondary succession in Japan. The mean seedling emergence times for all species were significantly increased to 23.6 and 32.2 d in the T2 and T4 plot compared to the Control plot, respectively. The most sensitive and insensitive species in seedling emergence time in T2 plot were O. biennis and D. adscendens, respectivel.y, and those in the T4 and CT4 plot were I. cylindrica and D. adscendens, E. crus-galli and A. virginicus, respectively. All experimental species showed no significant difference in the seedling emergence rate between treatments except for O. biennis and I. cylindrica. O. biennis showed a great decrease in the seedling emergence rate from 83.3% in the Control plot to 38.0%, 14.7%, and 29.3% in the T2, T4, and CT4 plot, respectively. Elevated $CO_2$ had very little effect on the seedling emergence. From these observations, it is expected that increased temperature would greatly advance the vegetative recovery time after disturbance through the advancement of seedling emergence time.

• Asian-Australasian Journal of Animal Sciences
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• 제10권1호
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• pp.122-133
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• 1997

Purified diets containing 5 graded levels of threonine were fed to young, growing and finishing pigs to determine the threonine requirement for growth and maintenance. A model was developed to subdivide the threonine requirement for the maintenance from the requirement for growth. From this model, the threonine requirement for growth was 7.733, 10.968 and 11.235 g/kg live weight gain and the maintenance requirement was 0.118, 0.048 and 0.024 g per unit of metabolic body size at each stage of growth, respectively. In the young pigs, the threonine requirement for growth was 0.388 g/g N gain and the maintenance requirement was 0.122 g per unit of metabolic body size. The breakpoint of plasma threonine concentrations was 3.995, 7.933 and 7.738 g/d, respectively. Expected requirements obtained from these formulae were in general agreement with previous estimates. Based on the weight gain vs N gain equation, about 4.24% of the retained protein was comprised of threonine and compared to 3.81%, the mean threonine content of pig muscle CP.

• Asian-Australasian Journal of Animal Sciences
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• 제10권1호
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• pp.86-97
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• 1997

Purified diets containing 5 graded levels of methionine + cystine were fed to young, growing and finishing pigs to determine the methionine + cystine requirement for growth and maintenance. A model was developed to subdivide the methionine + cystine requirement for maintenance from requirement for growth. From this model, the methionine + cystine requirement for growth was 8.633, 10.260 and 9.293 g/kg live weight gain and the maintenance requirement was 0.049, 0.016 and 0.019 g per unit of metabolic body size at each stage of growth, respectively. In the young pigs, the methionine + cystine requirement for growth was 0.491 g/g N gain and the maintenance requirement was 0.059 g per unit of metabolic body size. The breakpoint of plasma methionine + cystine concentrations was 3.888, 6.935 and 8.116 g/d, respectively. Expected requirements obtained from these formulae were in general agreement with previous estimates. Based on the weight gain vs N gain equation, about 4.44% of the retained protein was comprised of methionine + cystine and compared to 3.31%, the mean methionine + cystine content of pig muscle CP.

• Asian-Australasian Journal of Animal Sciences
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• 제10권1호
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• pp.54-63
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• 1997

Purified diets containing 5 graded levels of lysine were fed to young and growing pigs to determine the lysine requirement for growth and maintenance. A model was developed to subdivide the lysine requirement for the maintenance from requirement for growth. From this model, the lysine requirement for growth was 18.018 and 19.431 g/kg live weight gain and the maintenance requirement was 0.115 and 0.033 g per unit of metabolic body size at each stage of growth, respectively. In the young pigs, the lysine requirement for growth was 0.950 g/g N gain and the maintenance requirement was 0.114 g per unit of metabolic body size. The breakpoint of plasma lysine concentrations was 8.695 and 13.464 g/d, respectively. Expected requirements obtained from these formulae were in general agreement with previous estimates. Based on weight gain vs N gain equation, about 7.92% of the retained protein was comprised of lysine as compared to 7.11%, the mean lysine content of pig muscle CP.

• Asian Journal of Atmospheric Environment
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• 제6권4호
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• pp.304-313
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• 2012

In this study, the ${\AA}$ngstrom exponent for polydispersed aerosol during dynamic processes was investigated. Log-normal aerosol size distribution was assumed, and a sensitivity analysis of the ${\AA}$ngstrom exponent with regards the coagulation and condensation process was performed. The ${\AA}$ngstrom exponent is expected to decrease because of the particle growth due to coagulation and condensation. However, it is difficult to quantify the degree of change. In order to understand quantitatively the change in the ${\AA}$ngstrom exponent during coagulation and condensation, different real and imaginary parts of the refractive index were considered. The results show that the ${\AA}$ngstrom exponent is sensitive to changes in size distribution and refractive index. The total number concentration decreases and the geometric mean diameter of aerosols increase during coagulation. On the while, the geometric standard deviation approaches monodispersed size distribution during the condensation process, and this change in size distribution affects the ${\AA}$ngstrom exponent. The degree of change in the ${\AA}$ngstrom exponent depends on the refractive index and initial size distribution, and the size parameter changes with the ${\AA}$ngstrom exponent for a given refractive index or chemical composition; this indicates that the size distribution plays an important role in determining the ${\AA}$ngstrom exponent as well as the chemical composition. Subsequently, this study shows how the ${\AA}$ngstrom exponent changes quantitatively during the aerosol dynamics processes for a log-normal aerosol size distribution for different refractive indices; the results showed good agreement with the results for simple analytic size distribution solutions.

• 한국습지학회지
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• 제14권4호
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• pp.503-518
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• 2012

지면에서 탄소수지를 모의하는 모형들은 주로 수직적인 탄소 플럭스에 주된 관심을 두어왔다. 반면에 수평 적인 탄소 플럭스에 관한 연구는 부족한 실정이다. 따라서 수평적인 탄소 플럭스의 흐름에 대해 연구하고자 유역 내 식생에 의해 생산되는 총 일차생산량(Gross primary production, GPP)과 유역에서 배출되는 총 유기탄소(Total Organic Carbon, TOC)의 교차상관분석을 실시하였다. 분석결과, GPP와 유역에서 배출되는 TOC의 유량가중 평균 농도는 서로 지체시간이 없을 경우에 가장 큰 상관관계를 보여주고 있음을 확인하였다. 또한 주기성이 고려된 상관구조를 살펴본 결과, 여름철의 TOC와 GPP 사이의 상관관계가 주기성이 고려되지 않았을 경우의 상관관계와 유사하게 나타났다. 이로부터 GPP와 TOC의 상관관계는 여름철에 맺어지는 GPP와 TOC 상관관계에 의해서 대부분이 규정되고 있음을 살펴볼 수 있었다. 이에 따라 식생에 의해 생산된 탄소와 유역에서 배출되는 유기탄소의 관계가 계절적으로도 긴밀한 관계가 있을 것이라 추측된다. 따라서 향후 연구는 계절에 따른 식생변수와 GPP와 TOC의 관계를 분석함으로서 조금 더 정량적으로 수평적인 탄소 플럭스 흐름에 대해 이해 할 수 있을 것으로 기대된다.

• 한국자동차공학회논문집
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• 제19권5호
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• pp.134-142
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• 2011

Although combustion is essential in most energy generation processes, it is one of the major causes of air pollution. Exhaust pipes with circular fin were designed to study the effect of cooling the recirculated exhaust gases (EGR) of Diesel engines on the chemical composition of the exhaust gases and the reduction in the percentages of pollutant emissions. The gases examined in this study were oxides of nitrogen (NOx), carbon dioxide ($CO_2$) and carbon monoxide (CO). In addition, $O_2$ concentration in the exhaust was measured. The designs adopted in this study were about exhaust pipes with solid and hollow fins around them direct surface force measurement in water using a nano size colloidal probe technique. The direct force measurement between colloidal surfaces has been an essential topic in both theories and applications of surface chemistry. As particle size is decreased from micron size down to true Carbon nano Colloid size (<10 nm), surface forces are increasingly important. Nano particles at close proximity or high solids loading are expected to show a different behavior than what can be estimated from continuum and mean field theories. The current tools for directly measuring interaction forces such as a surface force apparatus or atomic force microscopy (AFM) are limited to particles much larger than nano size. This paper use Water and CNC fluid at normal cooling system of EGR. Experimental result showed all good agreement at Re=$2.54{\times}10^4$ by free convection and Re=$3.36{\times}10^4$ by forced air furnace.