• 제목/요약/키워드: Excitation-correlation

검색결과 134건 처리시간 0.027초

Plasmonic Nanosheet towards Biosensing Applications

  • Tamada, Kaoru
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2013년도 제44회 동계 정기학술대회 초록집
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    • pp.105-106
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    • 2013
  • Surface plasmon resonance (SPR) is classified into the propagating surface plasmon (PSP) excited on flat metal surfaces and the local surface plasmon (LSP) excited by metalnanoparticles. It is known that fluorescence signals are enhanced by these two SPR-fields.On the other hand, fluorescence is quenched by the energy transfer to metal (FRET). Bothphenomena are controlled by the distance between dyes and metals, and the degree offluorescence enhancement is determined by the correlation. In this study, we determined thecondition to achieve the maximum fluorescence enhancement by adjusting the distance of ametal nanoparticle 2D sheet and a quantum dots 2D sheet by the use of $SiO_2$ spacer layers. The 2D sheets consisting of myristate-capped Ag nanoparticles (AgMy nanosheets) wereprepared at the air-water interface and transferred onto hydrophobized gold thin films basedon the Langmuir-Schaefer (LS) method [1]. The $SiO_2$ sputtered films with different thickness (0~100 nm) were deposited on the AgMy nanosheet as an insulator. TOPO-cappedCdSe/CdZnS/ZnS quantum dots (QDs, ${\lambda}Ex=638nm$) [2] were also transferred onto the $SiO_2$ films by the LS method. The layered structure is schematically shown in Fig. 1. The result of fluorescence measurement is shown in Fig. 2. Without the $SiO_2$ layer, the fluorescence intensity of the layered QD film was lower than that of the original QDs layer, i.e., the quenching by FRET was predominant. When the $SiO_2$ thickness was increased, the fluorescence intensity of the layered QD film was higher than that of the original QDs layer, i.e., the SPR enhancement was predominant. The fluorescence intensity was maximal at the $SiO_2$ thickness of 20 nm, particularly when the LSPR absorption wavelength (${\lambda}=480nm$) was utilized for the excitation. This plasmonic nanosheet can be integrated intogreen or bio-devices as the creation point ofenhanced LSPR field.

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GFRP retrofitting effect on the dynamic characteristics of model steel structure

  • Tuhta, Sertac
    • Steel and Composite Structures
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    • 제28권2호
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    • pp.223-231
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    • 2018
  • Nowadays, there are a great number of various structures that have been retrofitted by using different FRP Composites. Due to this, more researches need to be conducted to know more the characteristics of these structures, not only that but also a comparison among them before and after the retrofitting is needed. In this research, a model steel structure is tested using a bench-scale earthquake simulator on the shake table, using recorded micro tremor data, in order to get the dynamic behaviors. Columns of the model steel structure are then retrofitted by using GFRP composite, and then tested on the Quanser shake table by using the recorded micro tremor data. At this stage, it is needed to evaluate the dynamic behaviors of the retrofitted model steel structure. Various types of methods of OMA, such as EFDD, SSI, etc. are used to take action in the ambient responses. Having a purpose to learn more about the effects of GFRP composite, experimental model analysis of both types (retrofitted and no-retrofitted models) is conducted to evaluate their dynamic behaviors. There is a provision of ambient excitation to the shake table by using recorded micro tremor ambient vibration data on ground level. Furthermore, the Enhanced Frequency Domain Decomposition is used through output-only modal identification. At the end of this study, moderate correlation is obtained between mode shapes, periods and damping ratios. The aim of this research is to show and determine the effects of GFRP Composite implementation on structural responses of the model steel structure, in terms of changing its dynamical behaviors. The frequencies for model steel structure and the retrofitted model steel structure are shown to be 33.916% in average difference. Finally, it is shown that, in order to evaluate the period and rigidity of retrofitted structures, OMA might be used.

부가수 질량을 고려한 외팔판의 고유진동 해석 (Natural Frequency Analysis of Cantilever Plates with Added Mass)

  • 장현길;노인식;홍창호;이창섭
    • 대한조선학회논문집
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    • 제50권1호
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    • pp.1-7
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    • 2013
  • The high-skewed and/or composite propellers of current interests to reduce the ship vibration and to increase the acoustic performance are likely to be exposed to the unexpected structural problems. One typical example is that the added mass effect on the propellers working in the non-uniform wake field reduces the natural frequency of the propeller leading to the resonance with the low-frequency excitation of the external forces. To avoid this resonance problem during the design stage, the technique of fluid-structure interaction has been developed, but the higher-order effect of the blade geometry deformation is not yet considered in evaluating the added mass effects. In this paper the fluid boundary-value problem is formulated by the potential-based panel method in the inviscid fluid region with the velocity inflow due to the body deformation, and the structural response of the solid body under the hydrodynamic loading is solved by applying the finite element method which implements the 20-node iso-parametric element model. The fluid-structure problem is solved iteratively. A basic fluid-sturcture interaction study is performed with the simple rectangular plates of thin thickness with various planform submerged in the water of infinite extent. The computations show good correlation with the experimental results of Linholm, et al. (1965).

초음파 도플러 신호를 이용한 음성 합성 (Speech synthesis using acoustic Doppler signal)

  • 이기승
    • 한국음향학회지
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    • 제35권2호
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    • pp.134-142
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    • 2016
  • 본 논문에서는 40 kHz 초음파 신호를 입 주변에 쏘고, 되돌아오는 초음파 신호를 이용해 음성신호를 합성하는 방법을 소개하고 성능을 평가하였다. 발성하고 있는 입주변에 초음파를 방사하게 되면, 입술, 턱, 뺨 등의 움직임으로 인한 변위로 도플러 현상이 발생하고, 이에 따라 반사 신호에는 본래의 주파수 성분과는 다른 도플러 주파수가 관찰되는데, 본 논문에서는 이러한 도플러 주파수를 이용하여 음성 파라메터를 추정하도록 하였다. 음성합성에 앞서서 초음파 도플러 신호와 음성 신호 간의 상관관계를 각 주파수 별로 분석하였으며, 이로부터 초음파 도플러 신호를 이용한 음성 신호의 합성 가능성을 살펴보았다. 변환에는 초음파 도플러의 정적, 동적 특성을 함께 반영한 특징 변수를 사용하였으며 결합-혼합 가우시안 기법을 이용하여 음성 파라메터로 변환하였다. 5명의 피 실험자를 이용한 음성 합성 실험에서 필터뱅크 에너지 값을 초음파신호의 특징변수로, LPC(Linear Predictive Coefficient) 켑스트럼 계수를 음성 변수로 사용하는 경우 가장 우수한 변환 성능을 나타내었다. 음성신호에서 추출한 여기신호를 이용하여 합성음을 생성하고, 이를 청취하였을 때 72.2 %의 평균 인식율이 얻어짐을 확인할 수 있었다.

덕트 팬의 동특성 연구 (Dynamic Characteristics of Ducted Fan: A Study)

  • 백상민;권재룡;이욱
    • 항공우주시스템공학회지
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    • 제11권6호
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    • pp.84-91
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    • 2017
  • 덕트 팬의 정지 비행시 동특성에 관한 연구를 수행하였다. 팬에 적용되는 블레이드에 대해서 단면 모델링을 수행하였으며, 로터 시스템의 구성요소들을 고려하여 해석 모델을 구축하였다. 로터의 회전수 및 피치각 조건에 따른 동특성 해석을 수행하였으며, 해석 결과 제안된 덕트 팬 시스템은 설계 운용범위 내에서 진동 증가와 같은 공탄성학적 불안정성이 적음을 확인하였다. 해석 절차의 검증을 위해 실제덕트 팬 회전 시험장치를 구성하였다. 기능 시험을 수행하여 덕트 팬 시험장치의 구성품간 거동 및 간섭 여부를 확인하였다. 동특성 시험을 위해 유압가진기를 사용하여 콜렉티브 가진을 하였으며, 비회전/회전시 블레이드의 고유진동수를 측정하였다. 시험 결과와 해석 결과의 비교를 통해 시험 장치와 해석 모델간의 상관관계가 잘 정립되어 있음을 확인하였다.

Chlorophyll a Fluorescence Parameters of Hulled and Hull-less Barley (Hordeum vulgare L.) DH Lines Inoculated with Fusarium culmorum

  • Warzecha, Tomasz;Skrzypek, Edyta;Adamski, Tadeusz;Surma, Maria;Kaczmarek, Zygmunt;Sutkowska, Agnieszka
    • The Plant Pathology Journal
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    • 제35권2호
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    • pp.112-124
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    • 2019
  • Barley worldwide is affected seriously by Fusarium seedling blight (FSB) and Fusarium head blight (FHB) diseases caused by the Fusarium species. The objective of this study was to facilitate the resistance of hulled and hull-less barley at different growth stages to F. culmorum according to direct parameters: disease rating (DR), fresh weight of leaves and roots, kernel weight per spike, kernel number per spike, plump kernels, and indirect parameters - chlorophyll a fluorescence (CF). Plate assay, greenhouse and field tests were performed on 30 spring barley doubled haploid (DH) lines and their parents infected with Fusarium culmorum. Direct parameters proved that hulled genotypes show less symptoms. Most studied chlorophyll a fluorescence (CF) parameters (apart from DIo/CS - amount of energy dissipated from PSII for laboratory test, TRo/CS - amount of excitation energy trapped in PSII reaction centers, ETo/CS - amount of energy used for electron transport and RC/CS - number of active reaction centres in the state of fully reduced PSII reaction center in field experiment) were significantly affected by F. culmorum infection. In all experiments, hulled genotypes had higher values of CF parameters compared to hull-less ones. Significant correlations were detected between direct and indirect parameters and also between various environments. It was revealed that ABS/CS, TRo/CS, and RC/CS have significant positive correlation in greenhouse test and field experiment. Significant correlations suggest the possibility of applying the CF parameters in selection of barley DH lines resistant to F. culmorum infection.

Case study of random vibration analysis of train-bridge systems subjected to wind loads

  • Zhu, Siyu;Li, Yongle;Togbenou, Koffi;Yu, Chuanjin;Xiang, Tianyu
    • Wind and Structures
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    • 제27권6호
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    • pp.399-416
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    • 2018
  • In order to reveal the independent relationship between track irregularity and wind loads, the stochastic characteristics of train-bridge coupling systems subjected to wind loads were investigated by the multi-sample calculation. The vehicle was selected as 23 degrees of freedom dynamical model, and the bridge was described by three-dimensional finite element model. It was assumed that the wind loads were random processes with strong spatial correlation, while the track irregularities were stationary random ones. As a case study, a high-speed train running on a cable-stayed bridge subjected to wind loads was studied. The effect of rail irregularities was deemed to be independent of the effect of wind excitations on the coupling system in the same wind circumstance for the same project, leading to the conclusion that the effect of wind loads and moving vehicle could be calculated separately. The variance results of the stochastic responses of vehicle-bridge coupling system under the action of wind loads and rail irregularities together were equivalent to the sum of the variance of the responses induced by each excitation. Therefore, when one of the input excitations is different, only the effect of changed loads needs to be assessed. Moreover, the new calculated results were combined with the effect of unchanged loads to present the stochastic response of coupling system subjected to the different excitations, reducing the cost of computations. The stochastic characteristics, the CFD (cumulative distribution function) of the coupling system with different wind velocities, vehicle speed, and vehicle marshalling were studied likewise.

Stochastic finite element based seismic analysis of framed structures with open-storey

  • Manjuprasad, M.;Gopalakrishnan, S.;Rao, K. Balaji
    • Structural Engineering and Mechanics
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    • 제15권4호
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    • pp.381-394
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    • 2003
  • While constructing multistorey buildings with reinforced concrete framed structures it is a common practice to provide parking space for vehicles at the ground floor level. This floor will generally consist of open frames without any infilled walls and is called an open-storey. From a post disaster damage survey carried out, it was noticed that during the January 26, 2001 Bhuj (Gujarat, India) earthquake, a large number of reinforced concrete framed buildings with open-storey at ground floor level, suffered extensive damage and in some cases catastrophic collapse. This has brought into sharp focus the need to carry out systematic studies on the seismic vulnerability of such buildings. Determination of vulnerability requires realistic structural response estimations taking into account the stochasticity in the loading and the system parameters. The stochastic finite element method can be effectively used to model the random fields while carrying out such studies. This paper presents the details of stochastic finite element analysis of a five-storey three-bay reinforced concrete framed structure with open-storey subjected to standard seismic excitation. In the present study, only the stochasticity in the system parameters is considered. The stochastic finite element method used for carrying out the analysis is based on perturbation technique. Each random field representing the stochastic geometry/material property is discretised into correlated random variables using spatial averaging technique. The uncertainties in geometry and material properties are modelled using the first two moments of the corresponding parameters. In evaluating the stochastic response, the cross-sectional area and Young' modulus are considered as independent random fields. To study the influence of correlation length of random fields, different correlation lengths are considered for random field discretisation. The spatial expectations and covariances for displacement response at any time instant are obtained as the output. The effect of open-storey is modelled by suitably considering the stiffness of infilled walls in the upper storey using cross bracing. In order to account for changes in soil conditions during strong motion earthquakes, both fixed and hinged supports are considered. The results of the stochastic finite element based seismic analysis of reinforced concrete framed structures reported in this paper demonstrate the importance of considering the effect of open-storey with appropriate support conditions to estimate the realistic response of buildings subjected to earthquakes.

테르페나딘 체내동태 연구를 위한 혈청 중 펙소페나딘의 HPLC 정량법 개발 및 검증 (Development and Validation of an HPLC Method for the Pharmacokinetic Study of Fexofenadine in Human)

  • 조혜영;강현아;김윤균;최후균;이용복
    • Journal of Pharmaceutical Investigation
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    • 제35권6호
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    • pp.437-443
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    • 2005
  • A rapid, selective and sensitive reversed-phase HPLC method for the determination of a major metabolite of terfenadine, fexofenadine, in human serum was developed, validated, and applied to the pharmacokinetic study of terfenadine. Fexofenadine and internal standard, haloperidol were extracted from human serum by liquid-liquid extraction with acetonitrile and analyzed on a $Symmetry^{TM}$ C8 column with the mobile phase of 1% triethylamine phosphate (pH 3.7)-acetonitrile (67:33, v/v, adjusted to pH 5.6 with triethylamine). Detection wavelength of 230 nm for excitation, 280 nm for emission and flow rate of 1.0 mL/min were fixed for the study. The assay robustness for the changes of mobile phase pH, organic solvent content, and flow rate was confirmed by $3^{3}$ factorial design using a fixed fexofenadine concentration (50 ng/mL) with respect to its peak area and retention time. In addition, the ruggedness of this method was investigated at three different laboratories using same quality control (QC) samples. This method showed linear response over the concentration range of 10-500 ng/mL with correlation coefficients greater than 0.999. The lower limit of quantification using 0.5 mL of serum was 10 ng/mL, which was sensitive enough for the pharmacokinetic studies of terfenadine. The overall accuracy of the quality control samples ranged from 95.70 to 114.58% for fexofenadine with overall precision (% C.V.) being 3.53-14.39%. The relative mean recovery of fexofenadine for human serum was 90.17%. Stability studies (freeze-thaw, short-term, extracted serum sample and stock solution) showed that fexofenadine was stable during storage, or during the assay procedure in human serum. However, the storage at $-70^{\circ}C$ for 4 weeks showed that fexofenadine was not stable. The peak area and retention time of fexofenadine were not significantly affected by the changes of mobile phase pH, organic solvent content, and flow rate under the conditions studied. This method showed good ruggedness (within 15% C.V.) and was successfully used for the analysis of fexofenadine in human serum samples for the pharmacokinetic studies of orally administered Tafedine tablet (60 mg as terfenadine) at three different laboratories, demonstrating the suitability of the method.

초고층건물의 성능평가를 위한 응답의존 시스템판별 및 모델향상 (Output-Only System Identification and Model Updating for Performance Evaluation of Tall Buildings)

  • 조순호
    • 한국지진공학회논문집
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    • 제12권4호
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    • pp.19-33
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    • 2008
  • 구조물에 고유한 진동수,모드형태 및 감쇠비 등과 같은 동적성능치를 추출하기 위하여 25층 및 42층 건물에 대하여 자연진동에 의한 동적계측실험을 수행하였다. 고려된 건물은 주요 횡하중 지지기구로서 코아벽체 혹은 전단벽체가 추가된 철근콘크리트건물이며, 입면 혹은 평면상으로 골조가 혼합된 구조형식을 나타낸다. 특히, 25층 건물은 측면에 위치한 코아벽체 이외에 상부로부터 내려오는 전단벽 구조가 4층 바닥이하에서 골조형식으로 전환되는 복잡한 구조이다. 이와 같은 이유 및 건물 주방향의 유사한 강성배치로 매우 근접하고 혼합된 모드형태가 예상되어 시스템판별 시 어려움이 예상된다. 현재까지 개발된 다양한 시스템판별법을 대상건물의 자연진동 실측기록에 적용하여 모달계수를 유도하였으며, 그 결과를 비교 분석하였다. 3개의 주파수영역 및 4개의 시간영역에 근거한 응답의존 시스템판별법이 고려되었다. 서로 다른 시스템판별법에 의하여 추출된 고유진동수 및 감쇠비는 대체로 상당한 일치를 보였으나, 모드형태는 사용된 방법에 따라 정도가 다르게 불일치를 나타냈다. 실험으로부터 추출한 성능치와 초기 유한요소해석 값을 비교해 본 결과 대상건물 모두 적어도 저차 3개의 고유진동수에서 2배 정도의 차이를 나타냈다. 실험과 해석결과의 일치를 위하여 몇몇 수동모델향상이 시도되었으며, 허용할 만한 결과를 획득하였다. 사용된 시스템판별법에 대하여 각자의 장, 단점에 대하여 기술하였으며, 본 연구와 같은 실제 대형구조물에 대하여 자동모델향상기법을 적용할 시 예상되는 문제점에 대하여 토의하였다.