• Title/Summary/Keyword: Examination stress

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Isolation, Characterization, and Use for Plant Growth Promotion Under Salt Stress, of ACC Deaminase-Producing Halotolerant Bacteria Derived from Coastal Soil

  • Siddikee, M.A.;Chauhan, P.S.;Anandham, R.;Han, Gwang-Hyun;Sa, Tong-Min
    • Journal of Microbiology and Biotechnology
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    • v.20 no.11
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    • pp.1577-1584
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    • 2010
  • In total, 140 halotolerant bacterial strains were isolated from both the soil of barren fields and the rhizosphere of six naturally growing halophytic plants in the vicinity of the Yellow Sea, near the city of Incheon in the Republic of Korea. All of these strains were characterized for multiple plant growth promoting traits, such as the production of indole acetic acid (IAA), nitrogen fixation, phosphorus (P) and zinc (Zn) solubilization, thiosulfate ($S_2O_3$) oxidation, the production of ammonia ($NH_3$), and the production of extracellular hydrolytic enzymes such as protease, chitinase, pectinase, cellulase, and lipase under in vitro conditions. From the original 140 strains tested, on the basis of the latter tests for plant growth promotional activity, 36 were selected for further examination. These 36 halotolerant bacterial strains were then tested for 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity. Twenty-five of these were found to be positive, and to be exhibiting significantly varying levels of activity. 16S rRNA gene sequencing analyses of the 36 halotolerant strains showed that they belong to 10 different bacterial genera: Bacillus, Brevibacterium, Planococcus, Zhihengliuella, Halomonas, Exiguobacterium, Oceanimonas, Corynebacterium, Arthrobacter, and Micrococcus. Inoculation of the 14 halotolerant bacterial strains to ameliorate salt stress (150 mM NaCl) in canola plants produced an increase in root length of between 5.2% and 47.8%, and dry weight of between 16.2% and 43%, in comparison with the uninoculated positive controls. In particular, three of the bacteria, Brevibacterium epidermidis RS15, Micrococcus yunnanensis RS222, and Bacillus aryabhattai RS341, all showed more than 40% increase in root elongation and dry weight when compared with uninoculated salt-stressed canola seedlings. These results indicate that certain halotolerant bacteria, isolated from coastal soils, have a real potential to enhance plant growth under saline stress, through the reduction of ethylene production via ACC deaminase activity.

The Development of Automatic Grease Lubricator Driven by Gear Mechanism with Controlled Operating Time (주유시간 조절이 가능한 기어 메커니즘 구동방식의 자동그리스주유기 개발)

  • Wang, Duck-Hyun;Lee, Kyu-Young;Lee, Sang-Hoon
    • Journal of the Korean Society for Precision Engineering
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    • v.23 no.2 s.179
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    • pp.199-206
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    • 2006
  • Automatic grease lubricator is equipment that provides adequate amount of fresh grease constantly to the shaft and the bearings of machines. It minimizes the friction heat and reduces the friction loss of machines to the least. This research is to develop automatic grease lubricator by gear driven mechanism with controlled operation time. The ultimate design of this equipment is to lubricate an adequate amount of grease by a simple switch clicking according to the advanced set cycle. The backlash of the gear was minimized to increase the output power. To increase the power of gear mechanism, the binding frequency and the thickness of the coil were changed. To control the rotating cycles of the main shaft according to its set numbers, different resistance and chips were used to design the circuit to controls electrical signals with pulse. The body of the lubricator was analyzed by stress analysis with different constructed angle. The stress analysis for differing loading pressures applied to the exterior body of grease lubricator due to the setup angle, was found that the maximum stress was distributed over the outlet part where the grease lubricator suddenly narrowed contracts. Digital mock-up was analyzed and the rapid prototyping(RP) trial products were tested with PCB circuit and grease. The evaluation of the outlet capacity for RP trial products was conducted, because the friction caused by the outlet on the wall surface was an important factor in the operation of the equipment. Finally, the finishing process was applied to decrease the roughness of the surface to a comparable level and was able to test the performance examination for the product.

A Study on the Physical Properties and Subjective Evaluation of the PTT[Poly(trimethylene terephthalate)] Fabric (PTT[Poly(trimethylene terephthalate)] 직물(織物)의 물리적(物理的) 특성(特性)및 주관적(主觀的) 평가(評價)에 관(關)한 연구(硏究))

  • Seo, Hyo-Jeong;Kim, Jong-Jun;Jeon, Dong-Won
    • Journal of Fashion Business
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    • v.7 no.4
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    • pp.121-128
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    • 2003
  • A new textile material, poly(trimethylene terephthalate) polymer, has been introduced to the textile industry. The structure of PTT is similar to the PET, while the tensile deformation and subsequent recovery property is better than that of PET. In this study, the physical and mechanical properties of textile woven fabrics made of PTT, PET, and nylon 6 yarns as the filling yarn were determined using the Kawabata Evaluation System (KES), including tensile, bending, shearing, compression, and surface related parameters. On top of these measurements, the subjective ratings by evaluators were performed on the fabric samples. From the examination of the stress-strain behavior of the yarn specimens focused on the recovery mode, it was evident that the PTT specimen developed lower stress at 3% elongation. The subsequent recovery curve showed that the PTT has less stress-decay rate than the other specimens, implying that the recovery behavior of the PTT is recommendable for the end-uses including stretchable textile materials, sports wears, etc. The KES bending rigidity(B) value of the PTT sample fabric was lower than that of the PET sample fabric. Subjective evaluation of the fabric samples by the evaluators on the descriptive word pair "soft - not soft" showed similar tendency with the KES B determination of the fabric samples.

Influence of the number of remaining teeth of on sleep time and stress of adults older than 40 years (40세 이상 성인의 수면시간과 스트레스가 잔존 치아 수에 미치는 영향)

  • Cho, Min-Jeong;Kim, Hae-Kyoung;Oh, Na-Rae
    • Journal of Digital Convergence
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    • v.14 no.7
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    • pp.225-232
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    • 2016
  • This paper aimed to study on the relevance of mental health and oral health. The subjects were recruited total 3,261 elderly of over 40 years old, using the Sixth Korea National Health and Nutrition Examination Survey(KNHANES). All the collected data were analyzed by chi-square test, t-test, and logistic regression using SPSS 23.0 version. Logistic regression was used to analyze the relationship of sleeping time or stress and the number of remaining teeth, and 95% confidence intervals were computed. Sleeping time odds ratio(OR) of the number of remaining teeth was 1.52 (95% confidence interval: 1.074-2.135) and Stress odds ratio(OR) was 2.39 (95% confidence interval: 1.855-3.090), but there is no statistically significant difference after adjusted age, gender and other variables.

Relationship between Health Status and Life Styles and Atopic Dermatitis in Adolescents (청소년 대상의 건강상태, 생활습관과 아토피 피부염의 관계)

  • So, Eun-Sun;Yeo, Ji-Young
    • Child Health Nursing Research
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    • v.18 no.3
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    • pp.143-149
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    • 2012
  • Purpose: The aim was to explore the relationship between health status/life style and prevalence of atopic dermatitis in adolescents. Methods: Data from 1,743 adolescents (aged 13 to 18 years) derived from the fourth Korea National Health and Nutrition Examination Survey (KNHANES) were analyzed using binary logistic regression and the classification and regression tree (CART) model. Results: The prevalence rate of atopic dermatitis was 10.3%. Third quartile income class (OR=2.19, 95% CI=1.20-4.00), fair and unhealthy self-rated health (OR=1.49, 95% CI=1.06-2.11; OR= 2.73, 95% CI=1.64-4.55), past or current smoker (OR=0.41, 95% CI=0.20-0.85; OR=0.44, 95% CI=0.21-0.92), and more than average stress (OR=1.43, 95% CI=1.03-1.99) were related with increased prevalence of atopic dermatitis. The high prevalence risk groups for atopic dermatitis were adolescents who perceived themselves to be healthy on self-rated health and had more than average stress and those who perceived themselves to be unhealthy and who never smoked. Conclusion: The results of the study indicate that health status, smoking, and stress are associated with a prevalence risk for adolescent atopic dermatitis in different degrees. Therefore, for control of atopic dermatitis, various interventions for smoking and stress need to be reinforced for the high prevalence atopic dermatitis groups.

Increased Frontal Gamma and Posterior Delta Powers as Potential Neurophysiological Correlates Differentiating Posttraumatic Stress Disorder from Anxiety Disorders

  • Moon, Sun-Young;Choi, Yoo Bin;Jung, Hee Kyung;Lee, Yoonji Irene;Choi, Soo-Hee
    • Psychiatry investigation
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    • v.15 no.11
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    • pp.1087-1093
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    • 2018
  • Objective Posttraumatic stress disorder (PTSD) is distinct from anxiety disorders in its etiology and clinical symptomatology, and was reclassified into trauma- and stressor-related disorders in DSM-5. This study aimed to find neurophysiological correlates differentiating PTSD from anxiety disorders using resting-state quantitative electroencephalography (qEEG). Methods Thirty-six patients with either PTSD or acute stress disorder and 79 patients with anxiety disorder were included in the analysis. qEEG data of absolute and relative powers and patients' medication status on the day of qEEG examination were obtained. Electrodes were grouped into frontal, central, and posterior regions to analyze for regional differences. General linear models were utilized to test for group differences in absolute and relative powers while controlling for medications. Results PTSD patients differed from those with anxiety disorders in overall absolute powers [F(5,327)=2.601, p=0.025]. Specifically, overall absolute delta powers [F(1,331)=4.363, p=0.037], and overall relative gamma powers [F(1,331)=3.965, p=0.047] were increased in PTSD group compared to anxiety disorder group. Post hoc analysis regarding brain regions showed that the increase in absolute delta powers were localized to the posterior region [F(1,107)=4.001, p=0.048]. Additionally, frontal absolute gamma powers [F(1,107)=4.138, p=0.044] were increased in PTSD group compared to anxiety disorder group. Conclusion Our study suggests increased overall absolute delta powers and relative gamma powers as potential markers that could differentiate PTSD from anxiety disorders. Moreover, increased frontal absolute gamma and posterior delta powers might pose as novel markers of PTSD, which may reflect its distinct symptomatology.

Influencing Factors for the Development of Metabolic Syndrome by the Number of Metabolic Syndrome Diagnostic Components in Korean Adolescents (청소년의 대사증후군 진단개수에 따른 영향요인 분석; 국민건강영양조사(2016) 자료 이용)

  • Oh, Hyunsook;Lee, Wonjae
    • The Journal of Korean Society for School & Community Health Education
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    • v.19 no.3
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    • pp.1-14
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    • 2018
  • Objectives: The purpose of this study was to investigate the prevalence of metabolic syndrome and to find related factors according to the number of metabolic syndrome diagnostic components in Korean adolescents. Methods: The subjects of this study were 469 Korean adolescents aged from 12 to 18 enrolled in the 2016 Korea National Health and Nutrition Examination Survey. Statistical package R 3.4.2 was used for programming to apply diagnostic criterion of adolescent metabolic syndrome and for the analysis of the data such as weighted frequent analysis, weighted mean analysis and complex sampling design logistic regression analysis. Results: For adolescents 12 to 18 years of age, 2.55% had more than 2(${\geq_-}3$), 9.88% had more than 1(${\geq_-}2$) and 33.17% had more than 0(${\geq_-}1$) metabolic syndrome diagnostic components. It has been found that risk factors for no less than 2 metabolic syndrome diagnostic components were higher body mass index and higher stress, and risk factors for no less than 1 were higher body mass index, younger teenager and female. Conclusion: Obesity is the primary risk factor for the development of adolescent metabolic syndrome. Female or younger teenager are more likely to have one or more metabolic syndrome diagnostic components, and higher stress develop to the risk level of having two or more metabolic syndrome diagnostic components. Therefore, it is important to focus on obesity and stress management for the prevention and control of Korean adolescent metabolic syndrome.

Ginsenoside compound K ameliorates palmitate-induced atrophy in C2C12 myotubes via promyogenic effects and AMPK/autophagy-mediated suppression of endoplasmic reticulum stress

  • Kim, Tae Jin;Pyun, Do Hyeon;Kim, Myeong Jun;Jeong, Ji Hoon;Abd El-Aty, A.M.;Jung, Tae Woo
    • Journal of Ginseng Research
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    • v.46 no.3
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    • pp.444-453
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    • 2022
  • Background: Compound K (CK) is among the protopanaxadiol (PPD)-type ginsenoside group, which produces multiple pharmacological effects. Herein, we examined the effects of CK on muscle atrophy under hyperlipidemic conditions along with its pro-myogenic effects. Further, the molecular pathways underlying the effects of CK on skeletal muscle have been justified. Methods: C2C12 myotubes were treated with palmitate and CK. C2C12 myoblasts were differentiated using CK for 4-5 days. For the in vivo experiments, CK was administered to mice fed on a high-fat diet for 8 weeks. The protein expression levels were analyzed using western blotting analysis. Target protein suppression was performed using small interfering (si) RNA transfection. Histological examination was performed using Jenner-Giemsa and H&E staining techniques. Results: CK treatment attenuated ER stress markers, such as eIF2a phosphorylation and CHOP expression and impaired myotube formation in palmitate-treated C2C12 myotubes and skeletal muscle of mice fed on HFD. CK treatment augmented AMPK along with autophagy markers in skeletal muscle cells in vitro and in vivo experiments. AMPK siRNA or 3-MA, an autophagy inhibitor, abrogated the impacts of CK in C2C12 myotubes. CK treatment augmented p38 and Akt phosphorylation, leading to an enhancement of C2C12 myogenesis. However, AMPK siRNA abolished the effects of CK in C2C12 myoblasts. Conclusion: These findings denote that CK prevents lipid-induced skeletal muscle apoptosis via AMPK/autophagy-mediated attenuation of ER stress and induction of myoblast differentiation. Therefore, we may suggest the use of CK as a potential therapeutic approach for treating muscle-wasting conditions associated with obesity.

The Relationship Between Marital Status of Alcohol Drinkers and Mental Health (음주자의 결혼상태에 따른 정신건강과의 관련성)

  • Jung Hyun Jung;Kim Seok Hwan
    • The Journal of Korean Society for School & Community Health Education
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    • v.24 no.3
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    • pp.23-35
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    • 2023
  • Objectives: The purpose of this study is to examine the marital status, drinking behavior, and mental health characteristics of alcohol consumers, and to explore the relationship between marital status and mental health among alcohol consumers. Methods: The research data utilized raw data from the 8th Korea National Health and Nutrition Examination Survey (2020), Second Round. A total of 3,713 participants were included in the final analysis. Data analysis was performed using the statistical software SPSS version 26.0. Results: The relationship between the study participants and mental health revealed that compared to those with a spouse, individuals who were divorced or separated had an odds ratio (OR) of 2.598 for 'depression' and an OR of 2.142 for 'suicidal thoughts'. In the case of 'stress perception', individuals who were divorced or separated had an OR of 3.434. In terms of gender, females had an OR of 4.867 for 'depression', an OR of 2.510 for 'suicidal thoughts', and an OR of 1.847 for 'stress perception', compared to males. Regarding age, the odds of 'depression' were 1.801 times higher in the 60 to 79 age group compared to the 18 to 39 age group. For smoking status, smokers had an OR of 2.812 for 'depression' compared to non-smokers. The OR for 'suicidal thoughts' was 3.047, and the OR for 'stress perception' was 1.463. However, the relationship between mental health and binge drinking frequency and quantity of alcohol consumed in a single session was not significant. Conclusion: The divorced, separated, or bereaved groups may be vulnerable to 'depression,' 'suicidal ideation,' and 'stress.' Therefore, there is an expectation to explore strategies for the development and enhancement of community-based mental counseling and health education support programs, aiming to improve the mental well-being and quality of life of individuals and the local community.

An Examination of the Possibility of Loving-Kindness and Compassion Meditation for Pregnant Women: A Preliminary Study (임부를 위한 자비명상 기반 중재의 가능성 탐색: 예비연구)

  • Kim, Min Jeong;Heo, Jeong Mun;Gim, Wan Suk
    • Stress
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    • v.25 no.2
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    • pp.145-154
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    • 2017
  • Loving-kindness and compassion meditation (LKCM) is known to be effective in improving mental health and interpersonal relationships. An exploratory pilot study was conducted with the aim of examining the feasibility of LKCM which known to be effective in improving mental health and interpersonal relationships for pregnant women. Measures of stress, depression, maternal fetal attachment, mindfulness and self-compassion of subjects (n=8) were obtained at baseline, and after a 4-weeks LKCM program to identify effectiveness and supplements. Based on a focus group interview, phenomenological analysis was also conducted. The results showed that improvement potential of psychological comfort, positive relationship with fetus as well as complementary points for session contents and home training. We hope that this study will broaden understanding for the development of LKCM for pregnant women and promote further research.