• The Plant Pathology Journal
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• 제33권1호
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• pp.9-20
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• 2017

Incidence of leaf blast on nursery plants and pitting disease on maturing banana bunches has been recorded in banana plantations during rainy season in Eastern India during 2014 to 2015. Taxonomical identification as well as DNA sequence analysis of the internal transcribed spacer region of fungus isolated from affected tissue culture derived plantlets and fruits confirmed the pathogen to be Pyricularia angulata Hashioka "in both the cases". Koch's postulates were proved on young plantlets as well as on maturing fruits of cv. Grand Naine under simulated conditions. Evolutionary history was inferred and presented for our P. angulata strain PG9001 with GenBank accession no. KU984740. The analysis indicated that the P. angulata is phylogenitically distinct from other related species related to both Pyricularia and Magnaporthe. Detailed symptoms of blast lesions on young leaves, transition leaves, mid rib, petioles, peduncle, maturing bunches, bunch stalks and cushions were documented. Notably, the distinct small pitting spots on maturing bunches reduced the visual appeal of mature fruits. Appearance of pitting symptoms on fruits in relation with age of fruits and their distribution pattern on bunch and fingers was also documented in detail. Further, the roles of transitory leaves, weed hosts, seasonality on disease occurrence have also been documented.

• Reproductive and Developmental Biology
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• 제39권2호
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• pp.43-47
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• 2015

Sirtuin proteins are evolutionary conserved Sir2-related $NAD^+$-dependent deacetylases and regulate many of cellular processes such as metabolism, inflammation, transcription, and aging. Sirtuin contains activity of either ADP-ribosyltransferase or deacetyltranfease and their activity is dependent on the localization in cells. However, the expression pattern of Sirtuins has not been well studied. To examine the expression levels of Sirtuins, RT-PCR was performed using total RNAs from various tissues including liver, small intestine, heart, brain, kidney, lung, spleen, stomach, uterus, ovary, and testis. Sirtuins were highly expressed in most of tissues including the testis. Immunostaining assay showed that Sirt1 and Sirt6 were mainly located in the nucleus of germ cells, spermatocytes, and spermatids in the seminiferous tubules, whereas Sirt2 and Sirt5 were exclusively present in the cytoplasm of germ cells and spermatocytes. Our results indicate that Sirtuins may function as regulators of spermatogenesis and their activities might be dependent on their location in the seminiferous tubules.

• 한국지능시스템학회논문지
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• 제14권5호
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• pp.610-616
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• 2004

생체 공학이나 신경생리학, 로봇공학에서는 인간의 2족 보행 메커니즘을 알아내는 것이 중요한 연구과제이며 그에 대한 연구 성과는 재활도구나 컴퓨터 애니메이션 및 인간형 로봇과 같은 다양한 응용분야에 있어서의 기초 기술로서 제공되어질 것을 기대하고 있다. 반면에 인간의 2보행 운동은 신경계와 역학계에 의한 복잡한 상호작용으로, 그 실현 메커니즘에 있어 신경계의 구체적인 제어방법에 관해서는 그 복잡성 때문에 아직 많은 부분이 불명확하게 남겨져 있다. 따라서 전문가에 의한, 매번 시행착오를 통해 신경계의 상세한 설계를 할 필요가 있다. 이 논문은 유전자 프로그래밍을 이용하여 신경계의 구조와 Parameter를 자동적으로 최적화하는 모델을 제안하고 시뮬레이션을 통해 타당성을 확인하였다.

• 한국공작기계학회논문집
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• 제16권1호
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• pp.47-52
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• 2007

A material mixing method to obtain an optimal topology for a structure in a thermal environment was suggested. This method is based on Evolutionary Structural Optimization(ESO). The proposed material mixing method extends the ESO method to a mixing several materials for a structure in the multicriteria optimization of thermal flux and thermal stress. To do this, the multiobjective optimization technique was implemented. The overall efficiency of material usage was measured in terms of the combination of thermal stress levels and heat flux densities by using a combination strategy with weighting factors. Also, a smoothing scheme was implemented to suppress the checkerboard pattern in the procedure of topology optimization. It is concluded that ESO method with a smoothing scheme is effectively applied to topology optimization. Optimal topologies having multiple thermal criteria for a printed circuit board(PCB) substrate were presented to illustrate validity of the suggested material mixing method. It was found that the suggested method works very well for the multicriteria topology optimization.

• The Plant Pathology Journal
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• 제36권4호
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• pp.364-377
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• 2020

Sugarcane is an important sugar crop contributes more than 80% of world sugar production. Mosaic, leaf fleck, and yellow leaf (YL) are the major viral diseases affecting sugarcane, amongst YL occurrence is widely reported in all the sugarcane growing countries. It is caused by Sugarcane yellow leaf virus (SCYLV) and detailed works were done on complete genome characterization, transmission, and management. However, in countries like Egypt, South Africa, Cuba, Mauritius and Hawaii, the disease was reported to the cause of sugarcane yellow leaf phytoplasma (SCYP) and/or SCYLV as single/combined infections. Hence, we have investigated in detail to identify the exact Candidatus phytoplasma taxon associated in Indian cultivars affected with YL. The sequencing results and the restriction fragment length polymorphism pattern of the PCR products using the universal phytoplasma primers confirmed presence of sugarcane grassy shoot (SCGS) phytoplasma (16SrXI group) in the YL-affected plants. Mixed infection of SCYLV and SCGS phytoplasma was estimated as 32.8% in YL affected plants. Evolutionary genetic relationship between SCYP and SCGS phytoplasma representatively taken from different countries showed that SCYP from South Africa and Cuba were diverged from others and had a highest similarity with SCGS phytoplasma. Although we wanted to identify SCYP from YL affected Indian sugarcane cultivars, the study clearly indicated a clear absence of SCYP in YL affected plants and we found SCYLV as the primary cause for the disease.

• 아시아태평양코퍼스연구
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• 제1권1호
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• pp.31-60
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• 2020

This paper investigates changes in relativization in Singapore English broadsheet newspapers from 1993 to 2016. One of the first diachronic studies in Singapore English (SgE), it also explores corresponding data from the diachronic Siena-Bologna (SiBol) news corpus. As SgE is in the endonormative stabilization phase in Schneider's (2007) Dynamic Model of postcolonial Englishes, divergence from British English (BrE) is to be expected. In this study, the dataset is a new Singapore English Newspaper (SEN) corpus compiled from local news articles in 1993, 2005 and 2016, and the corpus tool employed is Sketch Engine. The results reveal changes in relativization practices in SEN over the given period, many of which occur in a similar pattern as those identified in SiBol, albeit at varying rates of change. Most significant of these include a sharp decline in the which relativizer in restrictive relative clauses with non-animate antecedents, complemented by a rise in that. The change has been so rapid that although which relative clauses were more common than that clauses in 1993, that has subsequently overtaken which for both the corpora. One shift in SEN that is different from SiBol is the increase in frequency of non-restrictive relative clauses in SgE. The likely motivators for the changes in the two varieties are identified as colloquialization, densification and prescriptivism. The effect each of these factors could have had on the varieties are discussed, as well as the implications that the findings have on our understanding of the evolutionary status of SgE as a postcolonial variety.

• 한국수산과학회지
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• 제28권6호
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• pp.699-707
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• 1995

Eurasian yellow perch (Perca fluviatilis) and American yellow perch (Perca flavescens) are known to be endemic species in Eurasia and North America, respectively. The presence of endemic species on each continent suggests their independent evolutionary history. However, because of the morphological similarity, distribution pattern, and only recent fossil record, their divergence time and speciation of the two Perca species has long been controversial. Here, from the comparison of the entire nucleotide sequences of cytochrome b gene, large genetic divergence between the two Perca species is observed although they are morphologically similar each other. Among 1,140 base pairs, interspecific nucleotide differences are found at 130 sites $(11.4\%)$. The differences varies with codon position, showing 22 sites in the first, 5 sites in the second, and 103 sites in the third codon position. Considering the types of nucleotide changes, transitional differences are much more than transversional differences and its ratio turned out to be 5.19. The estimated divergence time of the two Perca species indicates that they were separated each other approximately in the late Miocene period, which implies the long history of speciation. With comparison of the inferred amino acid sequences, strong structural and functional constraints which seem to be maintained by the highly conservative amino acid residues or protein regions, as found in other taxonomic groups of organisms, are also recognized in the cytochrome b of the fishes examined.

• Asian-Australasian Journal of Animal Sciences
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• 제21권11호
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• pp.1535-1543
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• 2008

Microsatellites (MS) are useful for quantifying genetic variation within and between populations and for describing the evolutionary relationships of closely related populations. The main objectives of this work were to estimate genetic parameters, measure genetic distances and reconstruct phylogenetic relationships between Australian Angora/Angora_Aus/ and Cashmere/Cashmere_Aus/ populations and three Mongolian Cashmere goat (Bayandelger/BD/, Zavkhan Buural/ZB/, and Gobi Gurvan Saikhan/GGS/) populations based on variation at fourteen MS loci. The level and pattern of observed and expected heterozygosity and polymorphic information content of the fourteen loci studied across the populations were quite similar and high. Except for SRCRSP07, all studied microsatellites were in Hardy-Weinberg Equilibrium (p<0.001). Moderate genetic variation (7.5%) was found between the five goat populations with 92.5% of total genetic variation attributable to diversity existing between the individuals within each population. The greatest Nei's genetic distances were found between the Angora and four Cashmere populations (0.201-0.276) and the lowest distances were between the Mongolian Cashmere goat populations (0.026-0.031). Compared with other Cashmere goat populations, the GGS (crossbred with Russian Don Goats) population had the smallest pairwise genetic distance from the Australian Angora population (0.192). According to a three-factorial correspondence analysis (CA), the three different Mongolian Cashmere populations could hardly be distinguished from each other.

• Journal of Plant Biology
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• 제35권3호
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• pp.205-210
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• 1992

한국산 나리속 7종 2변종에 대하여 광학현미경과 주사전자현미경으로 화분의 크기와 형태를 조사하였으며 이들의 진화경향성 및 계통을 고찰하였다. 이상의 조사 결과 한국산 나리속 식물은 2유형으로 구분할 수 있었다. 즉, 소망상형은 화분립의 크기와 망강의 크가가 비교적 작은 유형으로 하늘나리와 땅나리에서만 관찰되었고, 대망상형은 화분립의 크기와 망강의 크기가 비교적 큰 유형으로 그 밖의 모든 분류군에서 관찰되었는네, 소망상형이 대망상형보다 원시적인 것으로 생각된다. 또한 화분형질에서 소망상형으로 관찰된 하늘나리와 땅나리는 개화방향은 다르지만 린편, 화피편의 형태가 유사하므로, 이들을 개화방향에 따라 다른 절로 취급한 Wilson(1925)의 체계보다는 종자, 린편의 형태 등의 형질에 의해 같은 절로 취급한 Comber(1949)의 체계가 보다 타당한 것으로 생각되며, 이들을 같은 아절로서 취급해야 한다고 생각한다.

• 한국동물학회지
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• 제35권2호
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• pp.211-218
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• 1992

한국산 노랑초파리 군에 속하는 8종의 유전적 유연관계를 밝히고자 생식적 격리 그리고 수용성 단백질을 전기영동법으로 분석하였다. 생리적 격리 실험에서 교배전격리 실험결과를 Watanane와 Kawanishi model에 근거하여 보면 D. aurario complex 3종 중 D. triauror물가 원시종이며 남 auraria는 팍생종으로 나타났다. 교배후 격리 실험에서 나. melonogaster와 D. simuions의 교배시, D. melonogoster를 수컷으로 하였을 때는 불임의 수컷만이, 또 D. melonogaster를 암컷으로 곯였을 때는 불임의 암컷만이 출현하였다. 그리고 남 ouraria complex 3종간의 교배에서는 수정 능력이 있는 수컷과 암컷이 출현하였는데, 이는 아직 남 auraria Complex가 semispecies단계에 있음을 나타내는 것이라 할 수 있다. SDS-PAGE로 분석한 노랑초파리 군 8종의 band pattern을 densitometer로 scanning한 결과 남 susu상가 가장 특이하였으며, TDE에 의한 유전적 거리(Aquadro and Avise's)는 남 auror지와 봉 triauror지사이가 0.155로 가장 낮았고, D. melonogaster와 고. mfa 사이가 0.422로 가장 높았다. 본 연구의 결과를 UPGMA법 뜨로 분석하면, 한국산 노랑초파리 군 8종은 4개의 아군으로 나뉘어지며 이들은 2개의 다른 큰무리로 구분되었는데, D. suzu가기 아군, D. lutescens의 아군, D. melanogoster와 봉 simulons의 아군이 속한 큰무리와, D. mfa,0. ouroria, D. biauroria 그리고 남 triourario가 속한 다른 큰무리로 나눌 수 있다.