• 한국항해항만학회지
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• 제32권5호
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• pp.355-361
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• 2008

스테레오 정합은 스테레오 시각 분야에서 가장 활발히 연구되는 분야이다. 본 논문에서는 물체의 위치 인식을 위한 유전 알고리즘을 이용한 스테레오 정합을 제안한다. 정합 환경을 최적화 문제로 간주하고 진화 전략을 이용하여 최적해를 탐색한다. 따라서, 유전 연산자는 스테레오 정합에 맞게 설계하였고 개체는 변위집단을 대표한다. 영상의 수평화소라인을 염색체로 간주하였다. 비용함수는 스테레오 정합에서 사용하는 일반적인 제약조건들의 조합이다. 비용함수가 명암도, 유사도, 변위 평활성으로 구성되었기 때문에 정합을 시도할 때 매 세대마다 이 모든 요소들을 한번에 다룬다. 염색체를 정의하기 위해 LoG연산자로 경계선을 추출하였으며 실험을 통하여 제안한 방법을 검증하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권1호
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• pp.179-182
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• 2013

Human cytochrome P4502E1 (CYP2E1) is a well-conserved xenobiotic-metabolizing enzyme expressed in liver, kidney, nasal mucosa, brain, lung, and other tissues. CYP2E1 is inducible by ethanol, acetone, and other low-molecular weight substrates and may mediate development of chemically-mediated cancers. CYP2E1 polymorphisms alter the transcriptional activity of the gene. This study was conducted in order to investigate the allele frequency variation in different populations of Andhra Pradesh. Two hundred and twelve subjects belonging to six populations were studied. Genotype and allele frequency were assessed through TaqMan allelic discrimination (rs6413419) and polymerase chain reaction-sequencing (-1295G>C and -1055C>T) after DNA isolation from peripheral leukocytes. The data were compared with other available world populations. The SNP rs6413419 is monomorphic in the present study, -1295G>C and -1055C>T are less polymorphic and followed Hardy-Weinberg equilibrium in all the populations studied. The -1295G>C and -1055C>T frequencies were similar and acted as surrogates in all the populations. Analysis of HapMap populations data revealed no significant LD between these markers in all the populations. Low frequency of $CYP2E1^*c2$ could be useful in the understanding of south Indian population gene composition, alcohol metabolism, and alcoholic liver disease development. However, screening of additional populations and further association studies are necessary. The heterogeneity of Indian population as evidenced by the different distribution of $CYP2E1^*c2$ may help in understanding the population genetic and evolutionary aspects of this gene.

• 복식문화연구
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• 제16권1호
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• pp.88-99
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• 2008

This study aims to analyze product samples of convergence of fashion and technology, as convergence is a major trend in the age of digital paradigm, and to propose desirable directions of development for technology convergence in fashion design in the future. With bibliographic research as well as internet search of the cases of development and commercialization of convergence of fashion and technology from the 1960s, when wearable computers were first introduced as an early development form of convergence, to the present, it becomes possible to classify these cases into three groups: functionally oriented one, aesthetically oriented one, and one that combines function and aesthetics. The pros and cons of each group of these cases are discussed and an evolutionary trend is observed from the earlier stage of convergence with functionally oriented cases which tried to achieve specific purposes of the time to the later stage of convergence with emphasis on both function and aesthetics which reflects the sensitivity of the general wearers. When three groups are plotted in the positioning map with one axis of function-aesthetics and the other of industry-fashion designer, however, all three are located outside of the two axes, suggesting these elements are not very well combined. In order for the products of the fashion-technology convergence in the future to receive favorable response from the consumers, it is necessary for the fashion industry to develop a close collaboration with companies of digital technology and the convergence products not only will have to provide functional benefits of the new technology but also to satisfy the aesthetic demands of the wearers.

• 정보처리학회논문지D
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• 제8D권6호
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• pp.853-860
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• 2001

최근의 객체지향 소프트웨어개발에서는 설계 및 유지보수와 관련된 많은 문제점들을 해결하기 위하여 클래스를 재설계하거나 클래스계층구조를 재구성하는 등 객체지향 소프트웨어에 대한 일련의 재이용 및 재구성기법이 사용되고 있다. 본 논문에서는 클래스계층구조의 재구성에 관한 정형적인 이론을 제공함으로써 클래스계층구조의 재구성에 관하여 보다 수월하게 이해하고 적용할 수 있도록 하였다. 구체적으로 본 논문에서는 객체지향 소프트웨어의 개발에 있어서 주요 골격이 되는 클래스계층구조를 평탄화시킨 형태로 정의한 평탄화된 클래스계층구조를 소개하고, 임의의 클래스계층구조를 평탄화된 형태로 변형시키기 위한 알고리즘을 제안하였다. 클래스계층구조를 평탄화함으로써 클래스계층구조상의 계승 및 집약관계가 각 인스턴스들에게 어떻게 사상되는가를 수월하게 파악할 수 있으며, 주어진 클래스계층구조로부터 생성가능한 객체를 그대로 유지보존할 수 있는 평탄화된 형태의 새로운 클래스계층구조를 구축할 수 있다. 평탄화된 클래스계층구조는 클래스계층구조를 재구성하여 객체지향 소프트웨어를 점증적으로 변화 발전시키거나 재이용함에 있어서 기초를 제공하는 등 중요한 역할을 수행한다.

• Animal Systematics, Evolution and Diversity
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• 제36권4호
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• pp.336-346
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• 2020

Mitochondrial genome is an important molecule for systematic and evolutionary studies in metazoans. The development of next-generation sequencing (NGS) technique has rapidly increased the number of mitogenome sequences. The process of generating mitochondrial genome based on NGS includes different steps, from DNA preparation, sequencing, assembly, and annotation. Despite the effort to improve sequencing, assembly, and annotation methods of mitogenome, the low quality and/or quantity sequence in the final map can still be generated through the work. Therefore, it is necessary to check and curate mitochondrial genome sequence after annotation for proofreading and feedback. In this study, we introduce the pipeline for sequencing and curation for mitogenome based on NGS. For this purpose, two mitogenome sequences of Dermatobranchus otome were sequenced by Illumina Miseq system with different amount of raw read data. Generated reads were targeted for assembly and annotation with commonly used programs. As abnormal repeat regions present in the mitogenomes after annotation, primers covering these regions were designed and conventional PCR followed by Sanger sequencing were performed to curate the mitogenome sequences. The obtained sequences were used to replace the abnormal region. Following the replacement, each mitochondrial genome was compared with the other as well as the sequences of close species available on the Genbank for confirmation. After curation, two mitogenomes of D. otome showed a typically circular molecule with 14,559 bp in size and contained 13 protein-coding genes, 22 tRNA genes, two rRNA genes. The phylogenetic tree revealed a close relationship between D. otome and Tritonia diomea. The finding of this study indicated the importance of caution and curation for the generation of mitogenome from NGS.

• 한국생물정보학회:학술대회논문집
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• 한국생물정보시스템생물학회 2003년도 제2차 연례학술대회 발표논문집
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• pp.26-51
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• 2003

Large scale protein interaction maps provide a new, global perspective with which to analyse protein function. PSIMAP, the Protein Structural Interactome Map, is a database of all the structurally observed interactions between superfamilies of protein domains with known three-dimensional structure in thePDB. PSIMAP incorporates both functional and evolutionary information into a single network. It makes it possible to age protein domains in terms of taxonomic diversity, interaction and function. One consequence of it is to predict the most important protein domain structure in evolution. We present a global analysis of PSIMAP using several distinct network measures relating to centrality, interactivity, fault-tolerance, and taxonomic diversity. We found the following results: ${\bullet}$ Centrality: we show that the center and barycenter of PSIMAP do not coincide, and that the superfamilies forming the barycenter relate to very general functions, while those constituting the center relate to enzymatic activity. ${\bullet}$ Interactivity: we identify the P-loop and immunoglobulin superfamilies as the most highly interactive. We successfully use connectivity and cluster index, which characterise the connectivity of a superfamily's neighbourhood, to discover superfamilies of complex I and II. This is particularly significant as the structure of complex I is not yet solved. ${\bullet}$ Taxonomic diversity: we found that highly interactive superfamilies are in general taxonomically very diverse and are thus amongst the oldest. This led to the prediction of the oldest and most important protein domain in evolution of lift. ${\bullet}$ Fault-tolerance: we found that the network is very robust as for the majority of superfamilies removal from the network will not break up the network. Overall, we can single out the P-loop containing nucleotide triphosphate hydrolases superfamily as it is the most highly connected and has the highest taxonomic diversity. In addition, this superfamily has the highest interaction rank, is the barycenter of the network (it has the shortest average path to every other superfamily in the network), and is an articulation vertex, whose removal will disconnect the network. More generally, we conclude that the graph-theoretic and taxonomic analysis of PSIMAP is an important step towards the understanding of protein function and could be an important tool for tracing the evolution of life at the molecular level.

• 대한약리학회지
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• 제24권1호
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• pp.1-10
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• 1988

카테콜아민 생합성에 관여하는 마지막 효소인 phenylethanolamine N-methyltransferase는 Norepinephrine을 epinephrine으로 전환시키는 중요한 효소이다. PNMT효소의 발현은 epinephrine 신경세포의 발현에 필수적이다. 따라서 PNMT유전자를 크로닝하여 그 구조를 결정하고, 유전자 발현연구를 하는 것은 상당히 중요한 일이다. 그러나 최근에 저자가 bovine cDNA를 처음으로 분리하여 그 구조를 보고한 것 외에는 아직까지 인간 PNMT cDNA나, 전체 genomic DNA의 분리 보고는 없다. 이에 저자들은 인간 PNMT유전자의 전체구조와 여러 종(species) 사이의 진화적인 관계를 규명하기 위해서 human genomic library(Charon 4A)를 만들고, 이 library 이용하여 bovine cDNA를 probe로 13.1 Kb길이의 genomic clone을 분리 크로닝하는데 성공하였다. 이 유전자는 두개의 EcoRI site가 포함되어 있어서, EcoRI제한효소에 의해서 7.5 Kb, 5.0 Kb,0.6 Kb로 분리되었으며, Southern과 dot blot 실험 에서 보면 5.0 Kb와 0.6 Kb에 exon이 흩어져 존재하고 있으며, 7.5 Kb는 flanking sequence로 판명되었다.

• 천문학회지
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• 제51권3호
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• pp.49-63
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• 2018

We carry out a study of Sub-Millimeter Galaxies (SMGs) in the AKARI NEP-Deep field using the James Clerk Maxwell Telescope (JCMT) SCUBA-2 $850{\mu}m$ source catalog, released as part of the SCUBA-2 Cosmology Legacy Survey (S2CLS) program. The SCUBA-2 $850{\mu}m$ map has a root mean square (rms) noise of $1.2mJy\;beam^{-1}$ and covers an area of $0.60degree^2$. We find four SMGs which have counterparts to Herschel sources with spectroscopic redshifts in the literature. In addition, three dust obscured galaxies (DOGs) detected in Herschel bands are selected as a comparison sample. We derive IR luminosities of SMGs using the CIGALE code, which are similar to those of high redshift SMGs from previous studies. The contribution of AGN to the total IR luminosity in SMGs (2%-11%) is smaller than the lower limit for the one in DOGs (19%-35%), which is consistent with the expectation from the evolutionary scenario of massive galaxies. We search for SMGs in overdense regions as protocluster candidates and investigate four regions, including candidates around three DOGs. Finally, we argue that follow-up spectroscopic observation for the NEP-Deep field will provide crucial information to understand the role of SMGs in the evolution of massive galaxies.

• 대한미생물학회지
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• 제35권3호
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• pp.263-271
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• 2000

A clinical isolate of Klebsiella pneumoniae K7746 produced the extended-spectrum ${\beta}$-lactamase (ESBL) SHV-12. A 6.6 kb BamHI fragment containing the $bla_{SHV-12}$ gene of K7746 strain was cloned into pCRScriptCAM vector resulting in the recombinant plasmid p7746-Cl. The restriction map of 3.6 kb inserted DNA and sequences immediately surrounding $bla_{SHV-12}$ of p7746-C1 were homologous to plasmid pMPA2a carrying $bla_{SHV-2a}$. In addition, both $bla_{SHV-12}$ and $bla_{SHV-2a}$ were expressed from a common hybrid promoter made of the -35 region derived from the left inverted repeat of IS26 and the -10 region from the $bla_{SHV}$ promoter itself. The results indicate that $bla_{SHV-12}$ and $bla_{SHV-2a}$ may have evolved from a common ancestor in the sequential order of $bla_{SHV-2a}$ first, followed by $bla_{SHV-12}$. Furthermore, by the PCR mapping method using primers corresponding to the IS26 and $bla_{SHV}$, the association between IS26 and $bla_{SHV}$ was studied in 12 clinical isolates carrying $bla_{SHV-2a}$, 27 clinical isolates carrying $bla_{SHV-12}$, and 5 reference strains carrying $bla_{SHV-1}$ to $bla_{SHV-5}$. All 39 strains carrying $bla_{SHV-2a}$ or $bla_{SHV-12}$ were positive by the PCR, providing confirmative evidence that IS26 has been involved in the evolution and dissemination of $bla_{SHV-2a}$ and $bla_{SHV-12}$. But 5 reference strains carrying $bla_{SHV-1}$ to $bla_{SHV-5}$ were negative by the PCR. Therefore, we concluded that the molecular evolutionary pathway of $bla_{SHV-2a}$ and $bla_{SHV-12}$ may be different from that of other $bla_{SHV-ESBL}$, e.g., $bla_{SHV-2}$, $bla_{SHV-3}$, $bla_{SHV-4}$, and $bla_{SHV-5}$.

• 자원환경지질
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• 제55권6호
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• pp.737-760
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• 2022

고준위 방사성 폐기물(High-level radioactive waste; HLW)의 지질처분을 위해서는 심부 지하 환경에 대한 이해가 선행되어야 하며, 이는 지질학적, 수리지질학적, 지구화학적, 지질공학적 조사를 통해 가능하다. 우리나라는 HLW의 지질처분을 계획하고 있으나, 심부 지하 환경의 지구화학적 특성에 관한 연구가 부족한 편이다. 이에 본 논문에서는 지질처분 부지 선정을 위한 지구화학적 조사를 중심으로 선진국의 심부 지하수 연구 동향을 살펴봄으로써 앞으로 국내 수리지구화학 분야의 연구 과제를 도출하는데 참고하고자 하였다. 해외 8개 국가(미국, 캐나다, 핀란드, 스웨덴, 프랑스, 독일, 일본, 스위스)의 심부 지하 환경 조사 방법 및 결과와 함께 지질처분 부지 결정 과정과 향후 연구 계획을 살펴본 결과, 해외 선진국에서는 심부 지하 환경의 지구화학적 특성화를 위해 지하수 및 난대수층 내 간극수의 수화학과 동위원소(예: SO₄^2-의 ³⁴S, ¹⁸O, DIC의 ¹³C, ¹⁴C, H₂O의 ²H, ¹⁸O), 균열 충전광물(fracture-filling minerals), 유기물, 콜로이드, 산화-환원 지시자(예: Eh, Fe²⁺/Fe³⁺, H₂S/SO₄^2-, NH₄⁺/NO₃^-) 등을 조사하고 있으며, 이들 지구화학 자료의 통합 해석을 통해 해당 심부 환경이 지질처분에 적합한지를 평가하였다. 국내의 경우, 인공신경망을 이용한 Self-Organizing Map(자기조직화 지도), 다변량 통계 기반 M3 모델링(지하수 혼합 모델), 반응-경로 모델(reaction path model) 등을 이용하여 심부 지하수의 수화학적 유형 분류 및 진화 패턴 규명, 천부 지하수 혼합 영향, 균열 충전광물과 지하수화학 사이의 관계를 규명한 바 있다. 그러나 지질처분 부지를 선정하는데 있어 과학적 근거를 확보하기 위해 중요한 기타 지구화학 자료(예: 동위원소, 산화-환원 지시자, 용존유기물)가 매우 부족한 현실이며, 따라서 최적의 지질 처분지를 찾기 위해서는 지역별/유형별 심부 지하수에 대한 지구화학적 자료 구축이 요구된다.